Articles

Clean room microbiome complexity impacts planetary protection bioburden

The Spacecraft Assembly Facility (SAF) at the NASA’s Jet Propulsion Laboratory is the primary cleanroom facility used in the construction of some of the planetary protection (PP)-sensitive missions developed b...

Authors: Ryan Hendrickson, Camilla Urbaniak, Jeremiah J. Minich, Heidi S. Aronson, Cameron Martino, Ramunas Stepanauskas, Rob Knight and Kasthuri Venkateswaran

Cognitive behavioral therapy for irritable bowel syndrome induces bidirectional alterations in the brain-gut-microbiome axis associated with gastrointestinal symptom improvement

There is growing recognition that bidirectional signaling between the digestive tract and the brain contributes to irritable bowel syndrome (IBS). We recently showed in a large randomized controlled trial that...

Authors: Jonathan P. Jacobs, Arpana Gupta, Ravi R. Bhatt, Jacob Brawer, Kan Gao, Kirsten Tillisch, Venu Lagishetty, Rebecca Firth, Gregory D. Gudleski, Benjamin M. Ellingson, Jennifer S. Labus, Bruce D. Naliboff, Jeffrey M. Lackner and Emeran A. Mayer

Regulation of blood–brain barrier integrity by microbiome-associated methylamines and cognition by trimethylamine N-oxide

Communication between the gut microbiota and the brain is primarily mediated via soluble microbe-derived metabolites, but the details of this pathway remain poorly defined. Methylamines produced by microbial m...

Authors: Lesley Hoyles, Matthew G. Pontifex, Ildefonso Rodriguez-Ramiro, M. Areeb Anis-Alavi, Khadija S. Jelane, Tom Snelling, Egle Solito, Sonia Fonseca, Ana L. Carvalho, Simon R. Carding, Michael Müller, Robert C. Glen, David Vauzour and Simon McArthur

Leave no stone unturned: individually adapted xerotolerant Thaumarchaeota sheltered below the boulders of the Atacama Desert hyperarid core

The hyperarid core of the Atacama Desert is an extremely harsh environment thought to be colonized by only a few heterotrophic bacterial species. Current concepts for understanding this extreme ecosystem are m...

Authors: Yunha Hwang, Dirk Schulze-Makuch, Felix L. Arens, Johan S. Saenz, Panagiotis S. Adam, Christof Sager, Till L. V. Bornemann, Weishu Zhao, Ying Zhang, Alessandro Airo, Michael Schloter and Alexander J. Probst

Minnesota peat viromes reveal terrestrial and aquatic niche partitioning for local and global viral populations

Peatlands are expected to experience sustained yet fluctuating higher temperatures due to climate change, leading to increased microbial activity and greenhouse gas emissions. Despite mounting evidence for vir...

Authors: Anneliek M. ter Horst, Christian Santos-Medellín, Jackson W. Sorensen, Laura A. Zinke, Rachel M. Wilson, Eric R. Johnston, Gareth G. Trubl, Jennifer Pett-Ridge, Steven J. Blazewicz, Paul J. Hanson, Jeffrey P. Chanton, Christopher W. Schadt, Joel E. Kostka and Joanne B. Emerson

Disentangling environmental effects in microbial association networks

Ecological interactions among microorganisms are fundamental for ecosystem function, yet they are mostly unknown or poorly understood. High-throughput-omics can indicate microbial interactions through associat...

Authors: Ina Maria Deutschmann, Gipsi Lima-Mendez, Anders K. Krabberød, Jeroen Raes, Sergio M. Vallina, Karoline Faust and Ramiro Logares

Remarkably coherent population structure for a dominant Antarctic Chlorobium species

In Antarctica, summer sunlight enables phototrophic microorganisms to drive primary production, thereby “feeding” ecosystems to enable their persistence through the long, dark winter months. In Ace Lake, a str...

Authors: Pratibha Panwar, Michelle A. Allen, Timothy J. Williams, Sabrina Haque, Sarah Brazendale, Alyce M. Hancock, David Paez-Espino and Ricardo Cavicchioli

Revealing the composition of the eukaryotic microbiome of oyster spat by CRISPR-Cas Selective Amplicon Sequencing (CCSAS)

The microbiome affects the health of plants and animals, including humans, and has many biological, ecological, and evolutionary consequences. Microbiome studies typically rely on sequencing ribosomal 16S RNA ...

Authors: Kevin Xu Zhong, Anna Cho, Christoph M. Deeg, Amy M. Chan and Curtis A. Suttle

Accelerated discovery of novel glycoside hydrolases using targeted functional profiling and selective pressure on the rumen microbiome

Carbohydrate-active enzymes (CAZymes) form the most widespread and structurally diverse set of enzymes involved in the breakdown, biosynthesis, or modification of lignocellulose that can be found in living org...

Authors: André L. A. Neves, Jiangkun Yu, Yutaka Suzuki, Marisol Baez-Magana, Elena Arutyunova, Eóin O’Hara, Tim McAllister, Kim H. Ominski, M. Joanne Lemieux and Le Luo Guan

Microbiota in mesenteric adipose tissue from Crohn’s disease promote colitis in mice

Mesenteric adipose tissue (mAT) hyperplasia, known as creeping fat is a pathologic characteristic of Crohn’s disease (CD). The reserve of creeping fat in surgery is associated with poor prognosis of CD patient...

Authors: Zhen He, Jinjie Wu, Junli Gong, Jia Ke, Tao Ding, Wenjing Zhao, Wai Ming Cheng, Zhanhao Luo, Qilang He, Wanyi Zeng, Jing Yu, Na Jiao, Yanmin Liu, Bin Zheng, Lei Dai, Min Zhi…

Xylan alleviates dietary fiber deprivation-induced dysbiosis by selectively promoting Bifidobacterium pseudocatenulatum in pigs

Low dietary fiber intake has been shown to disturb the gut microbiome community, damage the mucus barrier, and promote pathogen susceptibility. However, little is known about the temporal response of the gut m...

Authors: Zhenyu Wang, Yu Bai, Yu Pi, Walter J. J. Gerrits, Sonja de Vries, Lijun Shang, Shiyu Tao, Shiyi Zhang, Dandan Han, Zhengpeng Zhu and Junjun Wang

Fecal microbiota transplantation protects rotenone-induced Parkinson’s disease mice via suppressing inflammation mediated by the lipopolysaccharide-TLR4 signaling pathway through the microbiota-gut-brain axis

Parkinson’s disease (PD) is a prevalent neurodegenerative disorder, displaying not only well-known motor deficits but also gastrointestinal dysfunctions. Consistently, it has been increasingly evident that gut...

Authors: Zhe Zhao, Jingwen Ning, Xiu-qi Bao, Meiyu Shang, Jingwei Ma, Gen Li and Dan Zhang

Honey bee genetics shape the strain-level structure of gut microbiota in social transmission

Honey bee gut microbiota transmitted via social interactions are beneficial to the host health. Although the microbial community is relatively stable, individual variations and high strain-level diversity have...

Authors: Jiaqiang Wu, Haoyu Lang, Xiaohuan Mu, Zijing Zhang, Qinzhi Su, Xiaosong Hu and Hao Zheng

Circadian dynamics of the teleost skin immune-microbiome interface

Circadian rhythms of host immune activity and their microbiomes are likely pivotal to health and disease resistance. The integration of chronotherapeutic approaches to disease mitigation in managed animals, ho...

Authors: Amy R. Ellison, David Wilcockson and Jo Cable

Polyamine metabolism links gut microbiota and testicular dysfunction

Male fertility impaired by exogenous toxins is a serious worldwide issue threatening the health of the new-born and causing infertility. However, the metabolic connection between toxic exposures and testicular...

Authors: Qi Zhao, Jian-Feng Huang, Yan Cheng, Man-Yun Dai, Wei-Feng Zhu, Xiu-Wei Yang, Frank J. Gonzalez and Fei Li

A fiber-deprived diet causes cognitive impairment and hippocampal microglia-mediated synaptic loss through the gut microbiota and metabolites

Cognitive impairment, an increasing mental health issue, is a core feature of the aging brain and neurodegenerative diseases. Industrialized nations especially, have experienced a marked decrease in dietary fi...

Authors: Hongli Shi, Xing Ge, Xi Ma, Mingxuan Zheng, Xiaoying Cui, Wei Pan, Peng Zheng, Xiaoying Yang, Peng Zhang, Minmin Hu, Tao Hu, Renxian Tang, Kuiyang Zheng, Xu-Feng Huang and Yinghua Yu

Correction to: Biogeographic traits of dimethyl sulfide and dimethylsulfoniopropionate cycling in polar oceans

Authors: Zhao-Jie Teng, Qi-Long Qin, Weipeng Zhang, Jian Li, Hui-Hui Fu, Peng Wang, Musheng Lan, Guangfu Luo, Jianfeng He, Andrew McMinn, Min Wang, Xiu-Lan Chen, Yu-Zhong Zhang, Yin Chen and Chun-Yang Li

Spatiotemporal variation of the indoor mycobiome in daycare centers

Children spend considerable time in daycare centers in parts of the world and are exposed to the indoor micro- and mycobiomes of these facilities. The level of exposure to microorganisms varies within and betw...

Authors: Eva Lena F. Estensmo, Luis Morgado, Sundy Maurice, Pedro M. Martin-Sanchez, Ingeborg B. Engh, Johan Mattsson, Håvard Kauserud and Inger Skrede

Correction to: Microbial growth and volatile organic compound (VOC) emissions from carpet and drywall under elevated relative humidity conditions

Authors: Sarah R. Haines, Emma C. Hall, Katarzyna Marciniak, Pawel K. Misztal, Allen H. Goldstein, Rachel I. Adams and Karen C. Dannemiller

Dietary calcium phosphate strongly impacts gut microbiome changes elicited by inulin and galacto-oligosaccharides consumption

Fructo-oligosaccharides (FOS), inulin, and galacto-oligosaccharides (GOS) are widely recognized prebiotics that profoundly affect the intestinal microbiota, including stimulation of bifidobacteria and lactobac...

Authors: Jori Fuhren, Markus Schwalbe, Jos Boekhorst, Christiane Rösch, Henk A. Schols and Michiel Kleerebezem

A simplified synthetic community rescues Astragalus mongholicus from root rot disease by activating plant-induced systemic resistance

Plant health and growth are negatively affected by pathogen invasion; however, plants can dynamically modulate their rhizosphere microbiome and adapt to such biotic stresses. Although plant-recruited protectiv...

Authors: Zhefei Li, Xiaoli Bai, Shuo Jiao, Yanmei Li, Peirong Li, Yan Yang, Hui Zhang and Gehong Wei

Strain-level analysis reveals the vertical microbial transmission during the life cycle of bumblebee

Microbial acquisition and development of the gut microbiota impact the establishment of a healthy host-microbes symbiosis. Compared with other animals, the eusocial bumblebees and honeybees possess a simple, r...

Authors: Qinzhi Su, Qinglin Wang, Xiaohuan Mu, Hao Chen, Yujie Meng, Xue Zhang, Li Zheng, Xiaosong Hu, Yifan Zhai and Hao Zheng

Utilizing a reductionist model to study host-microbe interactions in intestinal inflammation

The gut microbiome is altered in patients with inflammatory bowel disease, yet how these alterations contribute to intestinal inflammation is poorly understood. Murine models have demonstrated the importance o...

Authors: Amy M. Tsou, Jeremy A. Goettel, Bin Bao, Amlan Biswas, Yu Hui Kang, Naresh S. Redhu, Kaiyue Peng, Gregory G. Putzel, Jeffrey Saltzman, Ryan Kelly, Jordan Gringauz, Jared Barends, Mai Hatazaki, Sandra M. Frei, Rohini Emani, Ying Huang…

Quantitative sequencing clarifies the role of disruptor taxa, oral microbiota, and strict anaerobes in the human small-intestine microbiome

Upper gastrointestinal (GI) disorders and abdominal pain afflict between 12 and 30% of the worldwide population and research suggests these conditions are linked to the gut microbiome. Although large-intestine...

Authors: Jacob T. Barlow, Gabriela Leite, Anna E. Romano, Rashin Sedighi, Christine Chang, Shreya Celly, Ali Rezaie, Ruchi Mathur, Mark Pimentel and Rustem F. Ismagilov

Metagenomic insights into the microbial communities of inert and oligotrophic outdoor pier surfaces of a coastal city

Studies of the microbiomes on surfaces in built environment have largely focused on indoor spaces, while outdoor spaces have received far less attention. Piers are engineered infrastructures commonly found in ...

Authors: Xinzhao Tong, Marcus H. Y. Leung, Zhiyong Shen, Justin Y. Y. Lee, Christopher E. Mason and Patrick K. H. Lee

Depth-dependent variability of biological nitrogen fixation and diazotrophic communities in mangrove sediments

Nitrogen-fixing prokaryotes (diazotrophs) contribute substantially to nitrogen input in mangrove sediments, and their structure and nitrogen fixation rate (NFR) are significantly controlled by environmental co...

Authors: Zhiwen Luo, Qiuping Zhong, Xingguo Han, Ruiwen Hu, Xingyu Liu, Wenjun Xu, Yongjie Wu, Weiming Huang, Zhengyuan Zhou, Wei Zhuang, Qingyun Yan, Zhili He and Cheng Wang

Gut microbiota response to antibiotics is personalized and depends on baseline microbiota

The magnitude of microbiota perturbations after exposure to antibiotics varies among individuals. It has been suggested that the composition of pre-treatment microbiota underpins personalized responses to anti...

Authors: Armin Rashidi, Maryam Ebadi, Tauseef Ur Rehman, Heba Elhusseini, Harika Nalluri, Thomas Kaiser, Shernan G. Holtan, Alexander Khoruts, Daniel J. Weisdorf and Christopher Staley

Intestinal microbiota shapes gut physiology and regulates enteric neurons and glia

The intestinal microbiota plays an important role in regulating gastrointestinal (GI) physiology in part through interactions with the enteric nervous system (ENS). Alterations in the gut microbiome frequently...

Authors: Fernando A. Vicentini, Catherine M. Keenan, Laurie E. Wallace, Crystal Woods, Jean-Baptiste Cavin, Amanda R. Flockton, Wendy B. Macklin, Jaime Belkind-Gerson, Simon A. Hirota and Keith A. Sharkey

Microbial growth and volatile organic compound (VOC) emissions from carpet and drywall under elevated relative humidity conditions

Microbes can grow in indoor environments if moisture is available, and we need an improved understanding of how this growth contributes to emissions of microbial volatile organic compounds (mVOCs). The goal of...

Authors: Sarah R. Haines, Emma C. Hall, Katarzyna Marciniak, Pawel K. Misztal, Allen H. Goldstein, Rachel I. Adams and Karen C. Dannemiller

Active virus-host interactions at sub-freezing temperatures in Arctic peat soil

Winter carbon loss in northern ecosystems is estimated to be greater than the average growing season carbon uptake and is primarily driven by microbial decomposers. Viruses modulate microbial carbon cycling vi...

Authors: Gareth Trubl, Jeffrey A. Kimbrel, Jose Liquet-Gonzalez, Erin E. Nuccio, Peter K. Weber, Jennifer Pett-Ridge, Janet K. Jansson, Mark P. Waldrop and Steven J. Blazewicz

Biogeographic traits of dimethyl sulfide and dimethylsulfoniopropionate cycling in polar oceans

Dimethyl sulfide (DMS) is the dominant volatile organic sulfur in global oceans. The predominant source of oceanic DMS is the cleavage of dimethylsulfoniopropionate (DMSP), which can be produced by marine bact...

Authors: Zhao-Jie Teng, Qi-Long Qin, Weipeng Zhang, Jian Li, Hui-Hui Fu, Peng Wang, Musheng Lan, Guangfu Luo, Jianfeng He, Andrew McMinn, Min Wang, Xiu-Lan Chen, Yu-Zhong Zhang, Yin Chen and Chun-Yang Li

Correction to: Modeling the temporal dynamics of cervicovaginal microbiota identifies targets that may promote reproductive health

Authors: Alexander Munoz, Matthew R. Hayward, Seth M. Bloom, Muntsa Rocafort, Sinaye Ngcapu, Nomfuneko A. Mafunda, Jiawu Xu, Nondumiso Xulu, Mary Dong, Krista L. Dong, Nasreen Ismail, Thumbi Ndung’u, Musie S. Ghebremichael and Douglas S. Kwon

Charting the complexity of the activated sludge microbiome through a hybrid sequencing strategy

Long-read sequencing has shown its tremendous potential to address genome assembly challenges, e.g., achieving the first telomere-to-telomere assembly of a gapless human chromosome. However, many issues remain...

Authors: Lei Liu, Yulin Wang, Yu Yang, Depeng Wang, Suk Hang Cheng, Chunmiao Zheng and Tong Zhang

Microbial colonization and resistome dynamics in food processing environments of a newly opened pork cutting industry during 1.5 years of activity

The microorganisms that inhabit food processing environments (FPE) can strongly influence the associated food quality and safety. In particular, the possibility that FPE may act as a reservoir of antibiotic-re...

Authors: José F. Cobo-Díaz, Adrián Alvarez-Molina, Elena A. Alexa, Calum J. Walsh, Oscar Mencía-Ares, Paula Puente-Gómez, Eleni Likotrafiti, Paula Fernández-Gómez, Bernardo Prieto, Fiona Crispie, Lorena Ruiz, Montserrat González-Raurich, Mercedes López, Miguel Prieto, Paul Cotter and Avelino Alvarez-Ordóñez

A meta-analysis study of the robustness and universality of gut microbiome-metabolome associations

Microbiome-metabolome studies of the human gut have been gaining popularity in recent years, mostly due to accumulating evidence of the interplay between gut microbes, metabolites, and host health. Statistical...

Authors: Efrat Muller, Yadid M. Algavi and Elhanan Borenstein

Recovery of strain-resolved genomes from human microbiome through an integration framework of single-cell genomics and metagenomics

Obtaining high-quality (HQ) reference genomes from microbial communities is crucial for understanding the phylogeny and function of uncultured microbes in complex microbial ecosystems. Despite improvements in ...

Authors: Koji Arikawa, Keigo Ide, Masato Kogawa, Tatsuya Saeki, Takuya Yoda, Taruho Endoh, Ayumi Matsuhashi, Haruko Takeyama and Masahito Hosokawa

Integration of spatio-temporal variations of surface metabolomes and epibacterial communities highlights the importance of copper stress as a major factor shaping host-microbiota interactions within a Mediterranean seaweed holobiont

Although considered as holobionts, macroalgae and their surface microbiota share intimate interactions that are still poorly understood. Little is known on the effect of environmental parameters on the close r...

Authors: Benoît Paix, Nicolas Layglon, Christophe Le Poupon, Sébastien D’Onofrio, Benjamin Misson, Cédric Garnier, Gérald Culioli and Jean-François Briand

Development of fungal-mediated soil suppressiveness against Fusarium wilt disease via plant residue manipulation

The development of suppressive soils is a promising strategy to protect plants against soil-borne diseases in a sustainable and viable manner. The use of crop rotation and the incorporation of plant residues i...

Authors: Xianfu Yuan, Shan Hong, Wu Xiong, Waseem Raza, Zongzhuan Shen, Beibei Wang, Rong Li, Yunze Ruan, Qirong Shen and Francisco Dini-Andreote

Successional dynamics and alternative stable states in a saline activated sludge microbial community over 9 years

Microbial communities in both natural and applied settings reliably carry out myriads of functions, yet how stable these taxonomically diverse assemblages can be and what causes them to transition between stat...

Authors: Yulin Wang, Jun Ye, Feng Ju, Lei Liu, Joel A. Boyd, Yu Deng, Donovan H. Parks, Xiaotao Jiang, Xiaole Yin, Ben J. Woodcroft, Gene W. Tyson, Philip Hugenholtz, Martin F. Polz and Tong Zhang

Distinct B cell subsets in Peyer’s patches convey probiotic effects by Limosilactobacillus reuteri

Intestinal Peyer’s patches (PPs) form unique niches for bacteria-immune cell interactions that direct host immunity and shape the microbiome. Here we investigate how peroral administration of probiotic bacterium

Authors: Hao-Yu Liu, Antoine Giraud, Cedric Seignez, David Ahl, Feilong Guo, John Sedin, Tomas Walden, Jee-Hwan Oh, Jan Peter van Pijkeren, Lena Holm, Stefan Roos, Stefan Bertilsson and Mia Phillipson

Metagenomic analysis of ancient dental calculus reveals unexplored diversity of oral archaeal Methanobrevibacter

Dental calculus (mineralised dental plaque) preserves many types of microfossils and biomolecules, including microbial and host DNA, and ancient calculus are thus an important source of information regarding o...

Authors: Lena Granehäll, Kun D. Huang, Adrian Tett, Paolo Manghi, Alice Paladin, Niall O’Sullivan, Omar Rota-Stabelli, Nicola Segata, Albert Zink and Frank Maixner

Gammaproteobacteria, a core taxon in the guts of soil fauna, are potential responders to environmental concentrations of soil pollutants

The ubiquitous gut microbiotas acquired from the environment contribute to host health. The gut microbiotas of soil invertebrates are gradually assembled from the microecological region of the soil ecosystem w...

Authors: Qi Zhang, Zhenyan Zhang, Tao Lu, Yitian Yu, Josep Penuelas, Yong-Guan Zhu and Haifeng Qian

Increasing the power of interpretation for soil metaproteomics data

Soil and sediment microorganisms are highly phylogenetically diverse but are currently largely under-represented in public molecular databases. Their functional characterization by means of metaproteomics is u...

Authors: Virginie Jouffret, Guylaine Miotello, Karen Culotta, Sophie Ayrault, Olivier Pible and Jean Armengaud

Single-colony sequencing reveals microbe-by-microbiome phylosymbiosis between the cyanobacterium Microcystis and its associated bacteria

Cyanobacteria from the genus Microcystis can form large mucilaginous colonies with attached heterotrophic bacteria—their microbiome. However, the nature of the relationship between Microcystis and its microbiome ...

Authors: Olga M. Pérez-Carrascal, Nicolas Tromas, Yves Terrat, Elisa Moreno, Alessandra Giani, Laisa Corrêa Braga Marques, Nathalie Fortin and B. Jesse Shapiro

Reduced B12 uptake and increased gastrointestinal formate are associated with archaeome-mediated breath methane emission in humans

Methane is an end product of microbial fermentation in the human gastrointestinal tract. This gas is solely produced by an archaeal subpopulation of the human microbiome. Increased methane production has been ...

Authors: Christina Kumpitsch, Florian Ph. S. Fischmeister, Alexander Mahnert, Sonja Lackner, Marilena Wilding, Corina Sturm, Anna Springer, Tobias Madl, Sandra Holasek, Christoph Högenauer, Ivan A. Berg, Veronika Schoepf and Christine Moissl-Eichinger

Multi-omics reveals the positive leverage of plant secondary metabolites on the gut microbiota in a non-model mammal

Flavonoids are important plant secondary metabolites (PSMs) that have been widely used for their health-promoting effects. However, little is known about overall flavonoid metabolism and the interactive effect...

Authors: Le Wang, Guangping Huang, Rong Hou, Dunwu Qi, Qi Wu, Yonggang Nie, Zhenqiang Zuo, Rui Ma, Wenliang Zhou, Yingjie Ma, Yibo Hu, Zhisong Yang, Li Yan and Fuwen Wei

Response and oil degradation activities of a northeast Atlantic bacterial community to biogenic and synthetic surfactants

Biosurfactants are naturally derived products that play a similar role to synthetic dispersants in oil spill response but are easily biodegradable and less toxic. Using a combination of analytical chemistry, 1...

Authors: Christina N. Nikolova, Umer Zeeshan Ijaz, Clayton Magill, Sara Kleindienst, Samantha B. Joye and Tony Gutierrez

Microbial river-to-sea continuum: gradients in benthic and planktonic diversity, osmoregulation and nutrient cycling

Coastal aquatic ecosystems include chemically distinct, but highly interconnected environments. Across a freshwater-to-marine transect, aquatic communities are exposed to large variations in salinity and nutri...

Authors: Hwee Sze Tee, David Waite, Gavin Lear and Kim Marie Handley

Trophic level drives the host microbiome of soil invertebrates at a continental scale

Increasing our knowledge of soil biodiversity is fundamental to forecast changes in ecosystem functions under global change scenarios. All multicellular organisms are now known to be holobionts, containing lar...

Authors: Dong Zhu, Manuel Delgado-Baquerizo, Jing Ding, Michael R. Gillings and Yong-Guan Zhu

Commensal microbe-derived acetate suppresses NAFLD/NASH development via hepatic FFAR2 signalling in mice

Non-alcoholic liver disease (NAFLD) is the hepatic manifestation of metabolic syndrome, and it can progress to non-alcoholic steatohepatitis (NASH). Alterations in the gut microbiome have been implicated in th...

Authors: Ryo Aoki, Masayoshi Onuki, Koya Hattori, Masato Ito, Takahiro Yamada, Kohei Kamikado, Yun-Gi Kim, Nobuhiro Nakamoto, Ikuo Kimura, Julie M. Clarke, Takanori Kanai and Koji Hase