- Open Access
A de novo approach to disentangle partner identity and function in holobiont systems
- Arnaud Meng†1Email authorView ORCID ID profile,
- Camille Marchet†2,
- Erwan Corre3,
- Pierre Peterlongo2,
- Adriana Alberti4,
- Corinne Da Silva4,
- Patrick Wincker4, 5,
- Eric Pelletier4, 5,
- Ian Probert6,
- Johan Decelle7,
- Stéphane Le Crom1,
- Fabrice Not8 and
- Lucie Bittner1Email author
© The Author(s). 2018
- Received: 15 November 2017
- Accepted: 13 May 2018
- Published: 9 June 2018
Study of meta-transcriptomic datasets involving non-model organisms represents bioinformatic challenges. The production of chimeric sequences and our inability to distinguish the taxonomic origins of the sequences produced are inherent and recurrent difficulties in de novo assembly analyses. As the study of holobiont meta-transcriptomes is affected by challenges invoked above, we propose an innovative bioinformatic approach to tackle such difficulties and tested it on marine models as a proof of concept.
We considered three holobiont models, of which two transcriptomes were previously published and a yet unpublished transcriptome, to analyze and sort their raw reads using Short Read Connector, a k-mer based similarity method. Before assembly, we thus defined four distinct categories for each holobiont meta-transcriptome: host reads, symbiont reads, shared reads, and unassigned reads. Afterwards, we observed that independent de novo assemblies for each category led to a diminution of the number of chimeras compared to classical assembly methods. Moreover, the separation of each partner’s transcriptome offered the independent and comparative exploration of their functional diversity in the holobiont. Finally, our strategy allowed to propose new functional annotations for two well-studied holobionts (a Cnidaria-Dinophyta, a Porifera-Bacteria) and a first meta-transcriptome from a planktonic Radiolaria-Dinophyta system forming widespread symbiotic association for which our knowledge is considerably limited.
In contrast to classical assembly approaches, our bioinformatic strategy generates less de novo assembled chimera and allows biologists to study separately host and symbiont data from a holobiont mixture. The pre-assembly separation of reads using an efficient tool as Short Read Connector is an effective way to tackle meta-transcriptomic challenges and offers bright perpectives to study holobiont systems composed of either well-studied or poorly characterized symbiotic lineages and ultimately expand our knowledge about these associations.
- De novo assembly
- k-mer based similarity
In its scientific acceptation, symbiosis is defined as the living together of unlike organisms whatever the nature of their relationship , ranging from parasitism to mutualism. Symbiosis is a widespread phenomenon in the biosphere and plays crucial roles in evolution and ecology. One of the most popular examples of mutualism is the interaction between fungi and land plants, where fungi form mycorrhizae that help land plants to retrieve nutrients from soil . In the ocean, benthic coastal ecosystems are structured and supported by symbiotic associations involving multipartners such as corals (Cnidaria, i.e., multicellular eukaryotes), microalgae (Dinophyceae, Symbiodinium spp., i.e. unicellular eukaryotes), and bacteria. Breakdown of this symbiosis ultimately leads to coral bleaching (the loss of photosynthetic symbionts), dramatically affecting the whole reef ecosystems . While coral bleaching has been largely studied, there is a growing evidence that partners involved in this holobiont system contribute to make coral reef persistent in oligotrophic seas . Symbiotic association between sponges (Porifera, i.e., multicellular eukaryotes) and bacteria (prokaryotes) allows bacteria to grow within the mesohyl matrix of the sponge where they can be metabolically active and persist in a highly oligotrophic habitat. The symbiotic interactions between sponges and bacteria are currently poorly understood from the genomic point of view . Symbiotic associations involving two unicellular eukaryotes are also widespread in the oceanic plankton [5–8]. For instance, the cosmopolitan mutualistic associations between heterotroph Radiolaria (host) and endosymbiotic microalgae play significant ecological and biogeochemical roles in the oceans , but the underlying genomic basis of such associations remains uncharacterized. Although not cultivable in vitro, extraction of nucleic acids is nevertheless possible on such symbiotic partnerships, and this has recently allowed shedding light on the identity of the partners and their co-evolutionary history [6, 7]. Several symbiotic microalgae have been identified using such molecular approaches, and many of them belong to the Dinophyta . However, mainly because of their highly complex and large genomes, the lack of reference genomes for both Dinophyta and Radiolaria make their study challenging for de novo assembly and functional annotation [10, 11].
As a proof of concept, we thus sorted the transcriptomic reads of these three holobiont models considering two major partners (i.e., using two reference libraries: one for the host and one for the symbiont), and then de novo assembled each of the subsets. We finally compared qualitatively and quantitatively the results (i.e., assembly metrics, functional and taxonomical annotations, presence of chimera) obtained when using SRC_c or not (Fig. 1; Additional file 1).
Disentangling the holobiont sequences
Performances of SRC_c
Cnidaria-Dinophyta holobiont (M1)
All symbionts library (M1a)
Symbiodinium spp. library (M1b)
Other symbionts library (M1c)
Porifera-Bacteria holobiont (M2)
Radiolaria-Dinophyta holobiont (M3)
SRC_c assignment results for the holobiont models M1, M2, and M3
% Reads from holobiont
Orbicella faveolata holobiont (M1a)
Assigned to host library
Assigned to symbiont library
Orbicella faveolata holobiont (M1b)
Assigned to host library
Assigned to symbiont library
Orbicella faveolata holobiont (M1c)
Assigned to host library
Assigned to symbiont library
Xestospongia muta holobiont (M2)
Assigned to host library
Assigned to symbiont library
Collozoum sp. holobiont (M3)
Assigned to host library
Assigned to symbiont library
With M1, SRC_c assigned 64.3% of the holobiont reads to the cnidarian host and 7.2% to the Dinophyta symbiont full library (analysis M1a, Table 2). Restricting the symbiont library to the genus Symbiodinium spp. sequences allowed obtaining similar results with 64.5% of the reads identified as specific to the host library and 7.1% as specific to the symbiont library (analysis M1b, Table 2). On the contrary, when Symbiodinium spp. is removed from the library, only 0.6% of the holobiont reads could be assigned to the symbionts, and the proportion of reads assigned to the host increases up to 67.3% (analysis M1c, Table 2). Our tests on the symbionts library showed that the library content affected drastically the reads retrieval by SRC_c and demonstrated the sensitivity of the strategy. Considering these results, we focused on the M1a dataset for downstream analyses. We also noticed that shared reads (i.e., found in both host and symbiont libraries) always represent the lowest proportion of holobiont reads (M1a, M2, and M3).
De novo assembly, contigs evaluation, and downstream analyses for M1 and M2
De novo assembly metrics and downstream analysis of SRC_c resulting subsets for holobiont models M1a, M2 and M3
% contigs in holobiont
Remapping rate (%)
# with ORFs
% of contigs with ORFs
Remapping rate of holobiont reads (%)
# predicted cds
% contigs with predicted cds
# annotated cds
% cds with functional annotations
Cnidaria-Dinophyta holobiont (M1a)
Porifera-Bacteria holobiont (M2)
Radiolaria-Dinophyta holobiont (M3)
To further test the usefulness of the reads sorting before the de novo assembly step, we compared the contigs assignment of M1a and M2 (column 1 in Table 3) with a usual taxonomic assignment performed with MEGAN6  (Additional file 1). For M1a, MEGAN6 assigned 71,143 contigs to the host Orbicella faveolata and 148,409 contigs to the symbiont Symbiodinium spp. (Additional file 2). All the contigs assigned to Orbicella faveolata with MEGAN6 were also found with the SRC_c strategy (Table 3), but our method assigned 19,415 more contigs to the host category. On the contrary, MEGAN6 assigned 21,197 additional contigs to Symbiodinium spp. compared to our categorization strategy (Table 3, Additional file 2). With M2, MEGAN6 assigned 11 contigs to the host Xestospongia muta (Additional file 2) which is far less than the 2654 contigs defined with the SRC_c strategy (Table 3). However, MEGAN6 assigned also 33,810 contigs to Amphimedon queenslandica, a distinct sponge species which is not supposed to be the host in this holobiont system. MEGAN6 also succeeded to assign more contigs to bacteria (21,318 contigs) than the SRC strategy (2431 contigs) (Table 3).
Our functional annotations were compared to the one from the original studies [24, 29] (Additional file 1), but as previous publications do not provide exhaustive lists of the functional annotations and their corresponding abundance, these comparisons are essentially qualitative. Moreover, to minimize the biases, we focused on the more abundant annotations. For the O. faveolata host (M1), similarities were found in the top 15 of the most abundant annotations (Additional file 3). At the biological processes level, both our study and  found abundant metabolic process GO term (GO:0008152; 819 CDs (coding sequences) and 5278 genes, respectively). At the molecular function level, our host contigs mainly corresponded to binding protein (GO:0005515; 36,349 CDs) while authors of  mainly found catalytic activity functions (GO:0003824; 3361 genes). For M2, rare overlaps are found between the study in  and our annotations (Additional file 3): at the biological processes level, 1 of the top 15 host annotations is identical (signal transduction (GO:0007165)), and 3 of the top 15 symbiont annotations are in common (metabolic process (GO:0008152); proton transport (GO:0015992) and protein folding (GO:0006457)).
Benchmark comparisons on M3: what difference does it make to use SRC_c?
For the holobiont model M3, we performed an entire assembly study of the yet unpublished meta-transcriptome, using the same assembly and annotation tools in order to compare, step by step, quantitatively and qualitatively the impact of using SRC_c (Additional file 1). In this way, assembly metrics, abundance of chimera, and functional contents were compared between the SRC_c contig sets (host, symbiont, shared, and unassigned) and a direct de novo assembled meta-transcriptome obtained from all holobiont reads (this strategy is hereafter called noSRC).
SRC_c impact on Radiolaria-Dinophyta holobiont model (M3)
# reads used in assembly
# assembled contigs
# predicted cds
# annotated cds
remapping rates (%)
Calculation time (min)
To test the usefulness of the categorization step, the M3 contigs from the noSRC strategy were taxonomically assigned using MEGAN6 (Additional files 1 and 5). MEGAN6 assigned 10 contigs to Collodaria, whereas 693 contigs were assigned to the host category by the SRC strategy. MEGAN6 assigned 1383 contigs to Dinophyceae compared to the 5207 contigs categorized as symbionts by the SRC strategy. The leftover MEGAN6 contigs were assigned to bacteria and Archeae (3799 contigs), viruses (76 contigs), and other-eukaryotes (29,524 contigs), and 127,447 contigs remained unassigned (162,947 unassigned contigs with the categorization using the SRC strategy).
The use of SRC_c to tackle meta-transcriptomic challenges
The strategy proposed here is a practical and scalable solution for transcriptomic assembly of non-model holobiont organisms, from which no or limited genomic information is available.
The present implementation of SRC_c  based on reference databases of putative partners involved in the holobiont consortium, and our analysis strategy, enabled the categorization of holobiont reads into four subsets. With respect to the reference libraries, as exemplified in M1, when the expected symbiotic partner (i.e., Symbiodinium spp.) is missing from the reference library, the number of reads assigned to the symbiont category decreases drastically from 50M reads to nearly 5M reads (Table 2). The M2 and M3 libraries do not contain reference data for the expected host partner, and consequently, only a low proportion of the holobiont reads are assigned to the host (19 and 3%, respectively). Accordingly, the proportion of unassigned reads is directly linked to both host and symbiont libraries content with respect to the studied holobiont. Overall, less unassigned reads were observed when the “correct” actors are involved (M1a: 24.4%) compared to the poorly studied models (M2: 61.6% and M3: 72.5%). These results highlight the sensitivity and specificity of the SRC_c requests that relies on the completeness of the database to accurately sort the reads of the holobiont. The SRC_c assignation step could be further improved by adding more sequences (i.e., reads, assembled genes, or transcripts) from taxonomically close species (from existing databases or newly produced) to the host and symbiont reference libraries, but also from multiple actors such as parasites and viruses that are common in multicellular and unicellular host cells. In this way, as SRC_c is a highly scalable tool, which has been improved since its first release  (Additional file 6), it is now possible and it will be relevant for future transcriptomic symbiosis studies to involve more than two reference libraries because symbiotic associations are often more intricate [27–31]. Involving more actors in the reference libraries will thus help to reduce step by step the proportion of unassigned holobiont reads.
We compared the SRC_c contigs metrics to those from previous studies (M1a and M2) [24, 29]. We found that not only our strategy allowed defining a new category of contigs (the “shared” contigs), but also allowed assembling more contigs than previous studies (Fig. 3). Our contigs metrics showed lower N50 for both models compared to previous studies but showed higher remapping rates overall for M1a (up to 90%, (Table 3)).
For M2, differences in the number of contigs as well as contigs metrics could be the results of the following: (i) the considered read set (we used the complete read set, whereas  used a reduced one, cf. details in the “Methods” section) and/or (ii) the use of distinct de novo assembly software (we used Trinity , whereas authors of  used the CLC workbench [CLC bio, Boston, MA, USA; (https://www.qiagenbioinformatics.com/)]. Previous studies had shown that Trinity is able to generate more assembled contigs than the CLC assembler when applied on the same dataset . It is also known that assembled contigs from Trinity are shorter than those assembled by CLC but provided similar proportion of significant hits against the nr database .
With M1a, our strategy produced 1.5 times more CDs with a functional annotation (Fig. 3). At that point, we are unable to tell whether this observation can be the consequence of a better suited assembly strategy (SRC_c treatment and/or assembly software), and/or the use of a different annotation pipeline, and/or the supplementation of reference annotation databases between 2015  and 2017.
With M3 analyses, we could estimate how SRC_c impacts the de novo assembly step and downstream analyses compared to a more conventional protocol (i.e., the noSRC strategy) (Table 4, Fig. 3, Additional file 1). The SRC strategy increased the total calculation time; however, compared to classic strategies, it allowed to create directly two subsets directly assigned to holobionts partners. Minimal differences were found between the SRC and noSRC strategies concerning the number of assembled contigs and, as for M1a and M2, the SRC strategy produced shorter contigs sequences with higher remapping rates. The SRC strategy helped to reduce importantly the number of potential chimeras. We conclude that the read sorting and assignation performed before the assembly step largely contributes to address one of the most delicate de novo assembly challenges . Overall, the use of SRC_c for M3 might not be indeed so impressive in terms of metrics improvement, but it ensures the production of high-quality de novo assembled sequences (i.e., with high remapping rate and without chimera), which is crucial when studying non-model organisms, and which is a real gain for biologists who plan to perform molecular experiment based on these newly produced sequences.
The SRC strategy offers new perspectives in functional annotations of holobiont partners
For all models, the SRC strategy led to a higher number of annotated contigs; however, as only partial information on the annotation content were provided separately for the host or the symbionts in previous publications [24, 29], we were mainly restricted to qualitative comparisons.
The comparison of the M1a host transcriptomes with the previous study meta-transcriptome  showed very few similarities for the most occurring functions, even if the most annotated function is common (i.e., metabolic process GO). Our 20 most occurring functions include signal transduction functions (14% of the total annotations) and molecule transport functions (8% of the total annotations) that do not appear in the most occurring function from . These newly highlighted functions could help better understand the Orbicella faveolata host with respect to communication and cellular exchanges with its partners. We were not able to perform a similar analysis for the symbiont transcriptome since authors from  only focused on the host transcriptome. For M2, only 1/15 and 3/15 common annotations were found for host and symbiont, respectively. We suggest that the divergences in the analytical pipeline used, here Trinity versus CLC for de novo assembly followed by InterProScan versus FastAnnotator for functional annotation, make the functional annotation contents hardly comparable between studies. Despite these discrepancies, results from both analyses must be considered as potentially valuable and have to be checked with genome alignment when available or through in vitro validation when considering restricted group of functions (e.g., PCR).
Symbioses involving single cell heterotrophic hosts and photosynthetic symbionts have been described in the oceanic plankton using morphological and molecular data [5–7, 15]. Radiolarians and their symbiotic microalgae (e.g., Haptophytes, Dinoflagellates) have an ecological and biogeochemical significance [42–45], but little is known about symbiosis establishment and maintenance. If most microalgal symbionts can be grown in the laboratory as free-living stage , the study of radiolarian host only relies on single-cell isolation from the field [35, 47]. In this study, the radiolarian host belongs to the Collodaria order which is ubiquitous and abundant in the open ocean [35, 48]. Our knowledge about their ecology and evolution is limited, and hence, our analyses represent an opportunity to learn more about the genetic repertoire of such uncultivable, non-model lineage. Regarding functional annotations, the SRC and the noSRC strategies provided very similar results but the SRC strategy categorized the functional annotations among four subsets (host, symbiont, shared, and unassigned) (Additional file 5), which can be explored independently, allowing group-specific interpretations and biological hypothesis building for each partner from the holobiont. For instance, symbiont CDs linked to the photosystem I and II were detected, confirming that SRC_c succeeded to assign reads to photosynthetic actors, as expected here for the symbiotic partner (Additional file 4).
Strategies regarding the use of SRC_c and future perspectives
SRC_c successfully compared different holobiont read sets to large reference libraries in less than 24 h, with reasonable computational resources (i.e., 10 CPUs and less than 20Go of RAM). By setting parameters (i.e., solidity threshold, k-mer size, similarity threshold), we adapted SRC_c to heterogeneous nature of sequences in libraries (i.e., length, raw reads or assembled genes/transcripts, data volume, k-mers distribution) and to poorly studied systems. When studying meta-transcriptome reads, selecting abundant k-mers helps to remove the one corresponding potentially to sequencing errors; however, rare sequence k-mers are consequently lost. On the contrary, when indexing already assembled sequences from genomes or transcriptomes, we do not expect a redundancy of the k-mers such as in high-throughput sequencing experiments, and we thus assume that any k-mer is relevant when it comes from a reference sequence.
Contrary to BLAST-like methods, SRC_c relies uniquely on shared k-mers for its similarity computation. It means that a certain amount of error-free k-mers (i.e., k-mers that do not contain sequencing errors) must be found in common in order to output sequences, which can make SRC_c less sensitive compared to alignment methods which authorize mismatches. However, contrary to alignment methods, SRC_c was tailored to scale to very high volume datasets, and comparisons presented in  showed that SRC_c could handle sets of orders of magnitudes higher volumes than BLAST (Additional file 6). SRC_c’s efficiency relies on its particular probabilistic data structure. The lightweight indexing and query of k-mers is made at the price of rare false positives. In our case, false positives correspond to k-mers that are not contained in the original indexed library. As in this work, the k-mer size was relatively low (i.e., 25), the default value for this parameter was kept ensuring a low false positives rate (Additional file 6). For longer k-mers (i.e., size > 31), we recommend to increase the size of the fingerprint if more precision is needed. SRC_c can also be used in a no-false positive mode that requires more memory, but that is still less costly than a hash table as demonstrated in .
In our tests, SRC_c helps to retrieve holobiont reads similar to host or symbiont close species. Previous tools like COMMET  already proposed such computation, although their data structure makes difficult the use of k-mers of small size, as computation time would be drastically impacted. SRC_c was thus chosen for its simple output and its adaptability to the heterogeneous nature of the libraries studied, notably by adapting the k-mer lowest occurrence and size parameters.
Future works on SRC_c parameters settings could include more extensive exploration of the impact of the similarity threshold parameter on the sensitivity of our approach. In this regard, if the reads similarity rate to the libraries could be relaxed, it may decrease the number of unassigned reads in particular for poorly studied models. A second strategy would be to implement an iterative enriching strategy to maximize the proportion of holobiont reads assigned to the host or to the symbiont. This strategy can allow to assign more sequences in the case of non-model organisms. After a first assignment round with SRC_c, holobiont reads linked to an identified group (host/symbiont) can be added to the reference libraries. Then, based on these new enriched libraries, a second run of SRC_c can be performed on the holobiont reads. This can be implemented as an iterative pipeline: at each round, more reads will be assigned to the host or symbiont categories and will then be used as reference libraries. Finally, the approach proposed here has been applied to holobiont systems (between two partners), but it could be used to address larger meta-transcriptomic datasets composed of more complex assemblages. Depending on the SRC_c library content, the user can choose to target either one or more specific species among the variety that composed such meta-transcriptomic datasets. Coupled to our assembly and downstream analysis strategy, the subsets resulting of the used of SRC_c are processed de novo allowing the potential discovery of newly assembled transcripts and the exploration of the functional and metabolic role for the first time of each partner without reference genome.
SRC_c successfully processed a variety of large-scale datasets and offered a pragmatic way to classify sequences from different holobiont partners before assembly. We showed that our strategy allows improving assembly metrics in some cases and, in any cases, ensured to reduce the number of chimeras and to produce high-quality newly de novo assembled sequences. Our approach offers an efficient strategy to assemble and study holobionts involving non-model organisms. Overall, this de novo approach, allowing a taxonomic categorization of functionalities, can reveal the link between identity and function, which is necessary to better understand the functioning and contribution of each partner in holobiont systems. Applying our strategy will thus provide new insights into future adaptative and evolutionary studies of the symbioses.
Radiolaria-Dinophyta holobiont model (M3) sampling, RNA-seq library and sequencing
The Collodaria colony was sampled in the South Pacific Ocean at the station 112.01 (coordinates in decimal degrees: latitude − 23.3, longitude − 133.9) during the Tara Oceans expedition in 2011 . The radiolarian colony of few centimeters in diameter was collected in situ at the subsurface (1 m deep) with a plastic jar, preventing disruption of the colony and aggregation of other planktonic organisms. Live observations through the binocular were performed to verify that no organisms were accidentally attached to the colony before preservation. The collected colony was directly isolated in 15 mL of RNAlater (ThermoFisher Scientific, Waltham, MA) and preserved at − 20 °C. Total RNA extraction was performed using NucleoSpin RNA kit (Macherey-Nagel, Düren, Germany) starting from a slice (about 1 cm diameter) of Collodaria PAC 37 colony. Briefly, frozen cells were transferred in a 1.5 mL tube containing 100 μL RA1 lysis buffer and grinded for 1 min with a motor driven pellet pestle previously refrigerated in liquid nitrogen. Then, 250 μL RA1 lysis buffer, previously mixed with 3.5 μL β-mercaptoethanol (1% of total RA1 volume), was added to the lysed cells, and the total volume was transferred to a Nucleospin filter. After centrifugation and addition of an equal volume of 70% ethanol, the RNA was purified following the manufacturer’s instructions and finally eluted in 40 μL nuclease-free water. Quantity and quality of extracted RNA were assessed by capillary electrophoresis on an Agilent Bioanalyzer (Agilent Technologies, Santa Clara, CA).
Finally, in order to reduce as far as possible the risk of residual genomic DNA, a further DNase treatment was applied on the total RNA using Turbo DNA-free kit (Thermo Fisher Scientific), according to the manufacturer’s protocol. After purification with the RNA Clean and Concentrator-5 kit (ZymoResearch, Irvine, CA), RNA was eluted in 10 μL nuclease-free water and used to synthetize cDNA with the Ovation RNA-seq System Version 2 (NuGEN, San Carlos, CA), following the manufacturer’s protocol. After cDNA shearing by Covaris E210 instrument (Covaris, Woburn, MA), Illumina library was prepared using the SPRIWorks Library Preparation System on a SPRI TE instrument (Beckmann Coulter Genomics, Danvers, MA), according to the manufacturer’s protocol without size selection. Ligation products were PCR-amplified using Illumina adapter-specific primers and Platinum Pfx DNA polymerase (ThermoFisher Scientific). After library profile analysis by Agilent 2100 Bioanalyzer and qPCR quantification (MxPro, Agilent Technologies), the library was sequenced using 101 base-length read chemistry in a paired-end flow cell on HiSeq2000 Illumina sequencer (Illumina, San Diego, CA), in order to obtain nearly 50 million paired end reads. Raw reads were deposited on the ENA database: https://www.ebi.ac.uk/ena/data/view/ERX2094044.
Data retrieval and sequence libraries construction
For each of the three holobiont models (Fig. 2), we built reference sequences libraries representing host and symbiont(s) by selecting the taxonomically closest organisms available in public datasets (Additional file 7).
For the Cnidaria-Dinophyta holobiont model (M1), the host library includes 20 assembled transcriptomes (466,582 contigs) of cnidarian organisms  (including data from the host species Orbicella faveolata itself) and 2 genome-derived ESTs (201,677 ESTs) of Nematostella vectensis and Orbicella faveolata . The symbiont library is composed of 123 RNA-seq reads datasets (a total of 5,563,498,607 reads) of Dinophyta (including the presumed major symbiont Symbiodinium spp. ) from the MMETSP project . We built three versions of the symbiont reference library, one composed of all Dinophyta (M1a), the second exclusively composed of Symbiodinium spp. (15 RNA-seq datasets, a total of 123,122,726 reads) (M1b), and the third composed of all Dinophyta except Symbiodinium spp. (108 RNA-seq datasets, a total of 5,440,375,881 reads) (M1c).
For the Porifera-Bacteria holobiont model (M2), four RNA-seq datasets of poriferan species were included in the host library (642,229,924 total reads): Amphimedon queenslandica , Crella elegans , and both Haliclona amboinensis and Haliclona tubifera . The M2 symbiont reference library corresponds to the Tara Oceans meta-genomic gene catalogue (OM-RGC) assembled from the pico-planktonic fractions (< 3 μm) including Eubacteria or Archaea . It is composed of the bacterial gene catalog (40,154,822 assembled gene sequences) which has been downloaded from the OM-RGC website (http://ocean-microbiome.embl.de/companion.html).
For the Radiolaria-Dinophyta holobiont model (M3), we gathered Rhizaria sequences from four de novo assembled holobionts: 7215 presumed host transcripts were extracted among a total of 15,404 de novo assembled transcripts . Host specific sequences were extracted from holobionts assemblies removing first sequences from prokaryotic origin with a BLASTn (e-value 1e-3) against the OM-RGC database, and second, removing symbionts sequences with a BLASTx (e-value 1e-3) against Dinophyta de novo assembled transcriptomes . The exhaustive Dinophyta library created for the M1a was used for the reference symbiont library.
All reference libraries described above include assembled transcriptomes, genomes, or RNA-seq raw read datasets for eukaryotic or prokaryotic holobiont partners (Additional file 7). Their sizes vary from 4.5 Mbp to 25 Gbp with sequences length from 100 bp to 84 Kbp (Additional file 7).
Comparing meta-transcriptomes (i.e., holobiont reads) to reference libraries using short read counter
Presentation of SRC_c
Short Read Connector Counter (SRC_c)  relies on a very lightweight data structure called a quasi-dictionary that enables to work with voluminous sequence sets. The quasi-dictionary enables to associate a piece of information to any element from a static set composed of N distinct elements. It is composed of two parts: a minimal perfect hash function (MPHF)  and a fingerprint table. The MPHF allows to index very efficiently the elements of the set in memory, such that each element can be associated to any piece of information (i.e., k-mer coverage, location in reads). The fingerprint table is used to verify the membership of an element to the indexed set of elements using the MPHF. This way, stranger elements to the MPHF can be filtered out. The quasi-dictionary is a probabilistic structure with a controlled false positive rate that depends on the size of the fingerprint. SRC_c needs as input two sets of sequences (that can be identical). To compare sequences from a query set Q to those from a target set T, the set indexed in the quasi-dictionary is a set of k-mers from T. Finally, for each sequence S from Q, the number of k-mers of S shared with T provides a similarity measure of S with the set T. This implies that the similarity measure given is asymmetrical: it depends on the placement of the k-mers on the reads of Q, not of those of T. SRC_c is available at https://github.com/GATB/short_read_connector, the commit 94aa6a65b5ddf61eba95108069fae29c41e51fb0 was used for this study.
Application on data
In this study, SRC_c is used to assign reads from a holobiont meta-transcriptome either to the host or to the symbionts. We divided the query of the holobiont dataset Q in two parts, one that consists in the comparison of Q reads to a bank (i.e., reference library) of host sequences and another that performs the comparison to a bank of symbiont sequences. The sets to index are composed of k-mers from the sequences. In each comparison, two sequence sets are considered: the whole holobiont set Q and the target bank set B. First, the set B, which contains reads or assembled sequences and represents sequences close to the host (resp. symbiont), is indexed. During the indexation phase, the solid set of k-mers (i.e., the set composed of any k-mer which occurrence is above a user-fixed threshold (the solidity threshold) in the data set) from T is computed using the DSK  method. This set is next indexed in the quasi-dictionary previously described. Then, the reads from the holobiont data set (Q) are queried. For each read, the query phase reports the abundance of its indexed k-mers. In the meantime, reads are checked to have enough positions (i.e., more than a given threshold which can be parameterized) for which an indexed k-mer starts over their length. This enables to add stringency to the query: a read that shares only a few k-mers with the index is considered not enough similar to the index. Finally, each read from Q (the holobiont) which was found similar to T (the host or the symbionts) during the query are returned in a binary vector and can be extracted to a FASTA format.
Parameters from SRC_c were carefully chosen. First, the solidity k-mer solidity threshold was adapted according to the nature of the sequences in the bank data set. For libraries for which sequences were shorter than 300 bp with a relatively high coverage (e.g., M1 symbiont library involved only reads), the default value was kept (solidity threshold = 2). For longer sequences (e.g., M1 host library was composed of ESTs and M2 symbiont library was composed of de novo assembled genes), the threshold was adapted and set to 1. Due to the presence of small reads (50 bp) in our holobiont datasets, we also modified the default k-mer size value of 31 to a value of 25, so that any read contains at least a few k-mers. Usually, the k-mer size is higher ; however, 25 base pairs correspond to a decent value to ensure the uniqueness of the read . During the query phase of SRC_c, a query sequence (from a dataset Q) must contain at least s% positions covered by at least one indexed k-mers (from a dataset B), to be considered similar to data from the set B . As the s default value is set to 50%, it means that a read of size l should have at least l × s positions covered by (overlapping or non-overlapping) indexed k-mers. Consequently, when a large majority of the reads could not be assigned, our strategy was to decrease the s parameter from 50 to 40 in order to increase the quantity of recalled reads. We set the similarity value s to 50% for M1 and M3 and decreased it to 40% for M2. Both query and indexation phases are parallelized in SRC_c. For this study, analyses were performed on a Linux system with 40 cores, with the option -t 0 (maximal number of available threads is used), and 250 GB of memory.
Read filtering, de novo assembly, and downstream analysis
For M1, M2, and M3 datasets, SortMeRNA  has been used with default parameters with the Silva 104 SSU and LSU nr reference databases, in order to estimate the proportion of reads corresponding to rRNA sequences. For M2, in comparison to the original publication in which the CLC workbench has been used and 41% of rRNA reads has been detected , we finally chose to consider the total read set (i.e., 16,818,599 more reads than ) in the assembly step as the rRNA detection with SortMeRNA detected only 8% or rRNA reads.
All read subsets resulting from the SRC_c step were first filtered (sequences trimming and cleaning) with the Trimmomatic program  (v0.36) and custom parameter SLIDINGWINDOW:10:20. Filtered reads were assembled using the de novo transcriptome assembly program Trinity  (v2.4.0) with default parameters. The newly assembled contigs metrics were calculated with the Transrate program  (v1.0.3). Additional downstream analyses include protein coding domain prediction using Transdecoder  (v3.0.1) and functional annotation with InterProScan 5  (v5.24-63), both with default parameters. The pipeline used for the steps described above is publicly available on a GitHub repository https://github.com/arnaudmeng/dntap .
Taxonomic assignment with MEGAN6
The contigs sequences were compared to the nr database (August 2017 version) with the DIAMOND software  (v0.28.22.84) using default parameters for BLASTx comparison and a e-value of 1e-3. The resulting alignments were processed with the daa2rma tool script provided with MEGAN6, and GeneInfo Identifier (GI) was mapped to alignments using the gi_taxid.bin file (version of May 2017). Finally, taxonomic assignment has been calculated with default parameters using the MEGAN LCA (Last Common Ancestor) algorithm and was visualized through the MEGAN6 software.
We followed the protocol described in . Fifty thousand randomly sampled de novo assembled contigs for the M3 (with the SRC strategy and without SRC strategy) were compared to the 7215 Rhizaria presumed contigs from  and 3,494,295 coding domains from de novo assembled contigs of 54 dinoflagellates transcriptomes . The comparison was made using the BLASTx program  (e-value 1e-3). The tools scripts detect_chimera_from_blastx.py from  was applied to resulting alignments to detect potential chimeras.
We thank the RCC staff for providing the dinoflagellates cultures as well as ABIMS staff for the help on computational facilities. This work was supported by a 3-year Ph.D. grant from the “Interface pour le Vivant” (IPV) program at the University of Pierre et Marie Curie (UPMC), Paris, France. This project was supported by Région Ile-de-France and benefited from the support of the project IMPEKAB ANR-15-CE02-0011.
Raw reads from the Collozoum sp. holobiont (M3) were deposited on the European Nucleotide Archive: https://www.ebi.ac.uk/ena/data/view/ERX2094044
LB and AM designed the analysis, and LB guided the study. IP, JD, and FN performed sampling and culture steps. AA and CDS optimized the molecular protocols and performed the sequencing analysis. AM and CM performed the computational analyses, with the help of PP and EC. AM, CM, PP, SLC, FN, and LB wrote the manuscript. EP and PW provided critical discussions. All authors read and approved the final manuscript.
The authors declare that they have no competing interests.
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
- De Bary A. De la symbiose. Rev Int Sci. 1879;3:301–9.Google Scholar
- Selosse M-A, Strullu-Derrien C. Origins of the terrestrial flora: a symbiosis with fungi? BIO Web Conf. 2015;4:00009.View ArticleGoogle Scholar
- Davy SK, Allemand D, Weis VM. Cell biology of cnidarian-dinoflagellate symbiosis. Microbiol Mol Biol Rev MMBR. 2012;76:229–61.View ArticlePubMedGoogle Scholar
- Nielsen DA, Petrou K, Gates RD. Coral bleaching from a single cell perspective. The ISME Journal. 2018;12:1558–67.Google Scholar
- Decelle J, Probert I, Bittner L, Desdevises Y, Colin S, de Vargas C, et al. An original mode of symbiosis in open ocean plankton. Proc Natl Acad Sci. 2012;109:18000–5.View ArticlePubMedPubMed CentralGoogle Scholar
- Probert I, Siano R, Poirier C, Decelle J, Biard T, Tuji A, et al. Brandtodinium gen. nov. and B. nutricula comb. Nov. (Dinophyceae), a dinoflagellate commonly found in symbiosis with polycystine radiolarians. J Phycol. 2014;50:388–99.View ArticlePubMedGoogle Scholar
- Mordret S, Romac S, Henry N, Colin S, Carmichael M, Berney C, et al. The symbiotic life of Symbiodinium in the open ocean within a new species of calcifying ciliate (Tiarina sp.). ISME J. 2016;10:1424–36.View ArticlePubMedGoogle Scholar
- Decelle J, Colin S, Foster RA. Photosymbiosis in marine planktonic protists. In: Marine protists. Tokyo: Springer; 2015. p. 465–500. https://doi.org/10.1007/978-4-431-55130-0_19.View ArticleGoogle Scholar
- Decelle J, Siano R, Probert I, Poirier C, Not F. Multiple microalgal partners in symbiosis with the acantharian Acanthochiasma sp. (Radiolaria). Symbiosis. 2012;58:233–44.View ArticleGoogle Scholar
- Sibbald SJ, Archibald JM. More protist genomes needed. Nat Ecol Evol. 2017;1:0145.View ArticleGoogle Scholar
- Hug LA, Baker BJ, Anantharaman K, Brown CT, Probst AJ, Castelle CJ, et al. A new view of the tree of life. Nat Microbiol. 2016;1:nmicrobiol201648.View ArticleGoogle Scholar
- Reuter JA, Spacek DV, Snyder MP. High-throughput sequencing technologies. Mol Cell. 2015;58:586–97.View ArticlePubMedPubMed CentralGoogle Scholar
- Muir P, Li S, Lou S, Wang D, Spakowicz DJ, Salichos L, et al. The real cost of sequencing: scaling computation to keep pace with data generation. Genome Biol. 2016;17:53.View ArticlePubMedPubMed CentralGoogle Scholar
- Shinzato C, Inoue M, Kusakabe M. A snapshot of a coral “holobiont”: a transcriptome assembly of the scleractinian coral, porites, captures a wide variety of genes from both the host and symbiotic zooxanthellae. PLoS One. 2014;9:e85182.View ArticlePubMedPubMed CentralGoogle Scholar
- Balzano S, Corre E, Decelle J, Sierra R, Wincker P, Da Silva C, et al. Transcriptome analyses to investigate symbiotic relationships between marine protists. Microb Physiol Metab. 2015;6:98.Google Scholar
- Daniels C, Baumgarten S, Yum LK, MIchell CT, Bayer T, Arif C, et al. Metatranscriptome analysis of the reef-building coral Orbicella faveolata indicates holobiont response to coral disease. Front Mar Sci. 2015;2. https://doi.org/10.3389/fmars.2015.00062.
- Bashiardes S, Zilberman-Schapira G, Elinav E. Use of metatranscriptomics in microbiome research. Bioinforma Biol Insights. 2016;10:19–25.View ArticleGoogle Scholar
- Li B, Fillmore N, Bai Y, Collins M, Thomson JA, Stewart R, et al. Evaluation of de novo transcriptome assemblies from RNA-Seq data. Genome Biol. 2014;15:553.View ArticlePubMedPubMed CentralGoogle Scholar
- Sangwan N, Xia F, Gilbert JA. Recovering complete and draft population genomes from metagenome datasets. Microbiome. 2016;4:8.View ArticlePubMedPubMed CentralGoogle Scholar
- Westreich ST, Korf I, Mills DA, Lemay DG. SAMSA: a comprehensive metatranscriptome analysis pipeline. BMC Bioinformatics. 2016;17:399.View ArticlePubMedPubMed CentralGoogle Scholar
- Martinez X, Pozuelo M, Pascal V, Campos D, Gut I, Gut M, et al. MetaTrans: an open-source pipeline for metatranscriptomics. Sci Rep. 2016;6:srep26447.View ArticleGoogle Scholar
- Mohsen H, Tang H, Ye Y. Improving de novo metatranscriptome assembly via machine learning algorithms. Int J Comput Biol Drug Des. 2017;10:91–107.View ArticleGoogle Scholar
- Marchet C, Limasset A, Bittner L, Peterlongo P. A resource-frugal probabilistic dictionary and applications in (meta)genomics. ArXiv160508319 Cs Q-Bio. 2016; http://arxiv.org/abs/1605.08319. Accessed 27 Jul 2017
- Pinzón JH, Kamel B, Burge CA, Harvell CD, Medina M, Weil E, et al. Whole transcriptome analysis reveals changes in expression of immune-related genes during and after bleaching in a reef-building coral. R Soc Open Sci. 2015;2 https://doi.org/10.1098/rsos.140214.
- Hoegh-Guldberg O. Climate change, coral bleaching and the future of the world’s coral reefs. Mar Freshw Res. 1999;50:839–66.View ArticleGoogle Scholar
- Muller-Parker G, D’Elia CF, Cook CB. Interactions between corals and their symbiotic algae. In: Coral reefs in the Anthropocene. Springer, Dordrecht; 2015. p. 99–116. doi:https://doi.org/10.1007/978-94-017-7249-5_5.
- Rohwer F, Seguritan V, Azam F, Knowlton N. Diversity and distribution of coral-associated bacteria. Mar Ecol Prog Ser. 2002;243:1–10.View ArticleGoogle Scholar
- Thompson JR, Rivera HE, Closek CJ, Medina M. Microbes in the coral holobiont: partners through evolution, development, and ecological interactions. Front Cell Infect Microbiol. 2015;4 https://doi.org/10.3389/fcimb.2014.00176.
- Fiore CL, Labrie M, Jarett JK, Lesser MP. Transcriptional activity of the giant barrel sponge, Xestospongia muta Holobiont: molecular evidence for metabolic interchange. Front Microbiol. 2015;6 https://doi.org/10.3389/fmicb.2015.00364.
- Webster NS, Taylor MW. Marine sponges and their microbial symbionts: love and other relationships. Environ Microbiol. 2012;14:335–46.View ArticlePubMedGoogle Scholar
- Simister RL, Deines P, Botté ES, Webster NS, Taylor MW. Sponge-specific clusters revisited: a comprehensive phylogeny of sponge-associated microorganisms. Environ Microbiol. 2012;14:517–24.View ArticlePubMedGoogle Scholar
- Siegl A, Kamke J, Hochmuth T, Piel J, Richter M, Liang C, et al. Single-cell genomics reveals the lifestyle of Poribacteria, a candidate phylum symbiotically associated with marine sponges. ISME J. 2011;5:61–70.View ArticlePubMedGoogle Scholar
- Webster NS, Luter HM, Soo RM, Botté ES, Simister RL, Abdo D, et al. Same, same but different: symbiotic bacterial associations in GBR sponges. Front Microbiol. 2013;3 https://doi.org/10.3389/fmicb.2012.00444.
- Hentschel U, Piel J, Degnan SM, Taylor MW. Genomic insights into the marine sponge microbiome. Nat Rev Microbiol. 2012;10:641–54.View ArticlePubMedGoogle Scholar
- Biard T, Pillet L, Decelle J, Poirier C, Suzuki N, Not F. Towards an integrative morpho-molecular classification of the Collodaria (Polycystinea, Radiolaria). Protist. 2015;166:374–88.View ArticlePubMedGoogle Scholar
- Guidi L, Chaffron S, Bittner L, Eveillard D, Larhlimi A, Roux S, et al. Plankton networks driving carbon export in the oligotrophic ocean. Nature. 2016;532:465–70.View ArticlePubMedPubMed CentralGoogle Scholar
- Grabherr MG, Haas BJ, Yassour M, Levin JZ, Thompson DA, Amit I, et al. Full-length transcriptome assembly from RNA-Seq data without a reference genome (Trinity). Nat Biotechnol. 2011;29:644–52.View ArticlePubMedPubMed CentralGoogle Scholar
- Huson DH, Auch AF, Qi J, Schuster SC. MEGAN analysis of metagenomic data. Genome Res. 2007;17:377–86.View ArticlePubMedPubMed CentralGoogle Scholar
- Marchet C, Lecompte L, Silva CD, Cruaud C, Aury JM, Nicolas J, et al. Clustering de novo by gene of long reads from transcriptomics data. bioRxiv. 2018:170035.Google Scholar
- Thanh NM, Jung H, Lyons RE, Njaci I, Yoon B-H, Chand V, et al. Optimizing de novo transcriptome assembly and extending genomic resources for striped catfish (Pangasianodon hypophthalmus). Mar Genomics. 2015;23:87–97.View ArticlePubMedGoogle Scholar
- Ungaro A, Pech N, Martin J-F, McCairns SR, Mevy J-P, Chappaz R, et al. Challenges and advances for transcriptome assembly in non-model species. bioRxiv. 2017:084145.Google Scholar
- Anderson OR. Radiolaria. Springer Science & Business Media; 2012.Google Scholar
- Murray SA, Suggett DJ, Doblin MA, Kohli GS, Seymour JR, Fabris M, et al. Unravelling the functional genetics of dinoflagellates: a review of approaches and opportunities. Perspectives in Phycology. 2016;3:37–52.Google Scholar
- Le Bescot N, Mahé F, Audic S, Dimier C, Garet M-J, Poulain J, et al. Global patterns of pelagic dinoflagellate diversity across protist size classes unveiled by metabarcoding. Environ Microbiol. 2016;18:609–26.View ArticlePubMedGoogle Scholar
- Biard T. Diversité, biogéographie et écologie des Collodaires (Radiolaires) dans l’océan mondial. 2016.Google Scholar
- Meng A, Corre E, Probert I, Gutierrez-Rodriguez A, Siano R, Annamale A, et al. Analysis of the genomic basis of functional diversity in dinoflagellates using a transcriptome-based sequence similarity network. Mol Ecol. https://doi.org/10.1111/mec.14579.
- Decelle J, Suzuki N, Mahé F, de Vargas C, Not F. Molecular phylogeny and morphological evolution of the Acantharia (Radiolaria). Protist. 2012;163:435–50.View ArticlePubMedGoogle Scholar
- Biard T, Stemmann L, Picheral M, Mayot N, Vandromme P, Hauss H, et al. In situ imaging reveals the biomass of giant protists in the global ocean. Nature. 2016;advance online publication; https://doi.org/10.1038/nature17652.
- Maillet N, Collet G, Vannier T, Lavenier D, Peterlongo P. Commet: comparing and combining multiple metagenomic datasets. In: 2014 IEEE international conference on bioinformatics and biomedicine (BIBM); 2014. p. 94–8.View ArticleGoogle Scholar
- Pesant S, Not F, Picheral M, Kandels-Lewis S, Bescot NL, Gorsky G, et al. Open science resources for the discovery and analysis of Tara Oceans data. Sci Data. 2015;2:sdata201523.View ArticleGoogle Scholar
- Bhattacharya D, Agrawal S, Aranda M, Baumgarten S, Belcaid M, Drake JL, et al. Comparative genomics explains the evolutionary success of reef-forming corals. elife. 2016;5Google Scholar
- Shinzato C, Shoguchi E, Kawashima T, Hamada M, Hisata K, Tanaka M, et al. Using the Acropora digitifera genome to understand coral responses to environmental change. Nature. 2011;476:320–3.View ArticlePubMedGoogle Scholar
- Schwarz JA, Brokstein PB, Voolstra C, Terry AY, Miller DJ, Szmant AM, et al. Coral life history and symbiosis: functional genomic resources for two reef building Caribbean corals, Acropora palmata and Montastraea faveolata. BMC Genomics. 2008;9:97.View ArticlePubMedPubMed CentralGoogle Scholar
- Keeling PJ, Burki F, Wilcox HM, Allam B, Allen EE, Amaral-Zettler LA, et al. The Marine Microbial Eukaryote Transcriptome Sequencing Project (MMETSP): illuminating the functional diversity of eukaryotic life in the oceans through transcriptome sequencing. PLoS Biol. 2014;12:e1001889.View ArticlePubMedPubMed CentralGoogle Scholar
- Fernandez-Valverde SL, Calcino AD, Degnan BM. Deep developmental transcriptome sequencing uncovers numerous new genes and enhances gene annotation in the sponge Amphimedon queenslandica. BMC Genomics. 2015;16:387.View ArticlePubMedPubMed CentralGoogle Scholar
- Pérez-Porro AR, Navarro-Gómez D, Uriz MJ, Giribet G. A NGS approach to the encrusting Mediterranean sponge Crella elegans (Porifera, Demospongiae, Poecilosclerida): transcriptome sequencing, characterization and overview of the gene expression along three life cycle stages. Mol Ecol Resour. 2013;13:494–509.View ArticlePubMedGoogle Scholar
- Guzman C, Conaco C. Comparative transcriptome analysis reveals insights into the streamlined genomes of haplosclerid demosponges. Sci Rep. 2016;6 https://doi.org/10.1038/srep18774.
- Sunagawa S, Coelho LP, Chaffron S, Kultima JR, Labadie K, Salazar G, et al. Structure and function of the global ocean microbiome. Science. 2015;348:1261359.View ArticlePubMedGoogle Scholar
- Limasset A, Rizk G, Chikhi R, Peterlongo P. Fast and scalable minimal perfect hashing for massive key sets. ArXiv170203154 Cs. 2017; http://arxiv.org/abs/1702.03154
- Rizk G, Lavenier D, Chikhi R. DSK: k-mer counting with very low memory usage. Bioinformatics. 2013;29:652–3.View ArticlePubMedGoogle Scholar
- Fofanov Y, Pettitt B, Li T, Tchoumakov S. Process and apparatus for using the sets of pseudo random subsequences present in genomes for identification of species. 2005. http://www.google.ch/patents/US20050255459.Google Scholar
- Kopylova E, Noé L, Touzet H. SortMeRNA: fast and accurate filtering of ribosomal RNAs in metatranscriptomic data. Bioinformatics. 2012;28:3211–7.View ArticlePubMedGoogle Scholar
- Bolger AM, Lohse M, Usadel B. Trimmomatic: a flexible trimmer for Illumina sequence data. Bioinformatics. 2014;30:2114–20.Google Scholar
- Smith-Unna R, Boursnell C, Patro R, Hibberd JM, Kelly S. TransRate: reference-free quality assessment of de novo transcriptome assemblies. Genome Res. 2016;26:1134–44.Google Scholar
- Haas BJ, Papanicolaou A, Yassour M, Grabherr M, Blood PD, Bowden J, et al. De novo transcript sequence reconstruction from RNA-seq using the Trinity platform for reference generation and analysis. Nat Protoc. 2013;8:1494–512.View ArticlePubMedGoogle Scholar
- Jones P, Binns D, Chang H-Y, Fraser M, Li W, McAnulla C, et al. InterProScan 5: genome-scale protein function classification. Bioinforma Oxf Engl. 2014;30:1236–40.View ArticleGoogle Scholar
- Buchfink B, Xie C, Huson DH. Fast and sensitive protein alignment using DIAMOND. Nat Methods. 2015;12:59–60.View ArticlePubMedGoogle Scholar
- Yang Y, Smith SA. Optimizing de novo assembly of short-read RNA-seq data for phylogenomics. BMC Genomics. 2013;14:328.View ArticlePubMedPubMed CentralGoogle Scholar
- Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. Basic local alignment search tool. J Mol Biol. 1990;215:403–10.View ArticlePubMedGoogle Scholar