A de novo approach to disentangle partner identity and function in holobiont systems

Microbiome

Table 3 De novo assembly metrics and downstream analysis of SRC_c resulting subsets for holobiont models M1a, M2 and M3

		# contigs	% contigs in holobiont	Smallest	Longest	N50	Mean length	% GC	Remapping rate (%)	# with ORFs	% of contigs with ORFs	Remapping rate of holobiont reads (%)	# predicted cds	% contigs with predicted cds	# annotated cds	% cds with functional annotations
Cnidaria-Dinophyta holobiont (M1a)	Host	90,558	15.66	201	29,214	1840	949	42	97.8	31,105	34.3	71.6	42,992	47.5	35,358	39
	Symbiont	127,212	22	201	13,093	1091	719	57	90.4	58,286	45.8	72.3	84,151	66.2	53,011	41.7
	Shared	46,017	7.96	201	7727	1067	796	55	82.3	28,075	61	41.4	38,547	83.8	25,382	55.2
	Unassigned	314,546	54.39	201	19,174	732	558	46	83.6	67,509	21.5	25.9	89,533	28.5	58,188	18.5
	Total	578,333								184,975			255,223		171,939
Porifera-Bacteria holobiont (M2)	Host	2654	2.33	201	1921	299	311	42	44.4	215	8.1	17.6	707	26.6	593	83.9
	Symbiont	2431	2.14	201	5001	406	396	46	25	411	16.9	4.7	1072	44.1	988	92.2
	Shared	2324	2.04	201	751	301	299	54	86.4	8	0.3	22.3	163	7	30	18.4
	Unassigned	106,377	93.49	201	8811	748	572	39	73.2	29,520	27.8	59.1	43,150	40.6	23,127	53.6
	Total	113,786								30,154			45,092		24,738	54.9
Radiolaria-Dinophyta holobiont (M3)	Host	693	0.41	201	1209	277	303	42	65.2	44	6.3	10.6	123	17.7	49	7.1
	Symbiont	5207	3.08	201	1777	324	328	54	76.2	618	11.9	32	1468	28.2	942	18.1
	Shared	52	0.03	201	639	298	308	39	81.3	0	0	18.6	6	11.5	5	9.6
	Unassigned	162,947	96.48	201	10,569	714	580	41	89.7	49,032	30.1	73.2	72,420	44.4	44,772	27.5
	Total	168,899								49,694			74,017		45,768

ISSN: 2049-2618