- Open Access
Transductomics: sequencing-based detection and analysis of transduced DNA in pure cultures and microbial communities
Microbiome volume 8, Article number: 158 (2020)
Horizontal gene transfer (HGT) plays a central role in microbial evolution. Our understanding of the mechanisms, frequency, and taxonomic range of HGT in polymicrobial environments is limited, as we currently rely on historical HGT events inferred from genome sequencing and studies involving cultured microorganisms. We lack approaches to observe ongoing HGT in microbial communities.
To address this knowledge gap, we developed a DNA sequencing-based “transductomics” approach that detects and characterizes microbial DNA transferred via transduction. We validated our approach using model systems representing a range of transduction modes and show that we can detect numerous classes of transducing DNA. Additionally, we show that we can use this methodology to obtain insights into DNA transduction among all major taxonomic groups of the intestinal microbiome.
The transductomics approach that we present here allows for the detection and characterization of genes that are potentially transferred between microbes in complex microbial communities at the time of measurement and thus provides insights into real-time ongoing horizontal gene transfer. This work extends the genomic toolkit for the broader study of mobile DNA within microbial communities and could be used to understand how phenotypes spread within microbiomes.
The importance of horizontal gene transfer (HGT) as a driver of rapid evolution and adaptation in microbial communities and host-associated microbiomes has become increasingly recognized [1, 2]. Publicly available genomes and metagenomes have revealed pervasive horizontally acquired genes in almost all available genomes. A study of HGT in the human microbiome, for example, showed > 10,000 recently transferred genes in 2235 analyzed genomes . HGT has been implicated in the spread of antibiotic resistance genes , toxin and other virulence genes [5, 6], as well as genes that enable digestion of dietary compounds by microbes in the intestine , and metabolic genes that augment microbial metabolism with critical functions in environmental populations . Despite its recognized importance, our understanding of the taxonomic range, frequency, and mechanisms of HGT are still limited. Most studies of HGT in microbiomes rely on analysis of microbial genomes [3, 9] and as such these methods attempt to reconstruct historical HGT. What we currently lack are methods that measure ongoing HGT and identify the mechanism of DNA transfer. Here, we present a novel method that specifically determines the sequence of DNA that is transferred between cells via one of the major known pathways for DNA transfer—transduction.
Currently, there are three major ways that genetic material is known to be exchanged between microbial cells: (1) transformation—uptake of DNA by naturally competent cells, (2) conjugation—exchange of genetic material (e.g., plasmids) using direct contact between donor and recipient cells, and (3) transduction—transfer of genetic material by viruses or virus-like particles (VLPs) . Here, we focus on transduction only. There are several known types of transduction including classic specialized and generalized transduction, and more recently discovered types, including gene transfer agents (GTAs), lateral transduction, and hijacking of bacteriophage (phage) particles by genomic islands [10,11,12]. During specialized transduction, DNA adjacent to prophage integration sites in the bacterial genome are co-excised at a low frequency and packaged into phage heads after prophage genome replication. In generalized transduction, non-random pieces of the host bacterial genomic DNA or plasmids get packaged at low frequency into phage particles when a lytic phage infects and replicates in a bacterial cell or upon induction of lysogens. This non-random packaging is mediated by genomic features that resemble the packaging site (pac site) on the phage genome, which is used by the phage particle packaging machinery as the start site of phage DNA packaging into the capsid . In lateral transduction, prophages replicate while still integrated in the host genome and prophage packaging initiates in situ ultimately leading to high frequency packaging of host DNA in a unidirectional fashion away from the prophage integration site . GTAs are phage-like particles encoded in bacterial genomes that package random pieces of the genomic DNA upon production and can transfer these pieces to other cells . In contrast to phages, GTAs do not carry the DNA content sufficient to support their reproduction in the target cells. Lastly, some genomic islands, including pathogenicity islands, can hijack phage capsids in an act of molecular piracy that enables their transduction [14, 15].
The unifying characteristic of all types of transduction is that virus or VLPs serve as the vector for transfer of genetic material between cells. Evidence so far indicates that these particles are abundant in most environments and that transduction occurs with a high frequency [16, 17]. However, approaches for measuring the abundance of transducing particles and transduction frequencies in microbiome samples are limited. These approaches usually rely on the application of cultured phage to environmental samples [16, 17] or sequencing of bacterial 16S rRNA genes from purified VLPs . The latter approach can determine which bacterial taxa’s DNA is carried in a VLP. However, the approach is limited to marker genes for which conserved PCR primer pairs exist and thus the majority of transduced DNA cannot be detected.
Here, we describe an unbiased approach, termed “transductomics,” which uses DNA sequencing to identify and characterize DNA originating from microbial cells that is carried in VLPs. This DNA is thus part of the pool of potentially transduced DNA termed the “transductome.” Our approach is based on two observations of transduced DNA in VLPs. First, transduced DNA often represents the genome of hosts that are present in the same sample as the VLPs. Therefore, if DNA from a microbe is found within VLPs purified from the same sample, this indicates a potential transduction event. Second, unique regions of the microbial host’s genome are unevenly enriched in the VLPs, as most mechanisms of transduction do not lead to random packaging of the host’s genome. In recent years, the uneven sequence coverage patterns produced by phages or GTAs carrying microbial host DNA have been used to characterize the genome biology and mechanisms of DNA packaging of specific host-phage/GTA systems [19,20,21,22]. Our transductomics approach exploits these sequence coverage patterns to identify and characterize transduced DNA in microbial communities. In the past, host DNA carried by VLPs may have been sequenced during metagenomic sequencing of purified VLPs, but without appropriate analysis tools these host-derived sequences were classified as host contamination of the VLP sample rather than being recognized as transduced DNA .
The transductomics approach that we present requires the sequencing of both the complete microbial community sample and VLPs that are ultra-purified using CsCl density gradient centrifugation from the same sample (Fig. 1). The VLP and complete sample sequencing reads are mapped to long genome contigs assembled from the complete sample metagenome. These contigs represent both microbial and viral genomes. Visualization of the read mapping coverages along the contigs comparing VLP and complete metagenome read coverages reveals patterns that can be associated with host DNA transport via VLPs. We demonstrate this method first using pure culture models of different transducing phages and other transducing particles. This is followed by the application of the approach to a murine intestinal microbiome community.
Results and discussion
Characterization of sequence coverage patterns associated with different transduction modes in model systems
Specialized transduction by Escherichia coli prophage λ
We used the well-studied specialized transducing bacteriophage λ to analyze the sequencing coverage patterns produced by specialized transduction. In specialized transduction, a prophage, which is integrated in the chromosome of the bacterial host, packages host genome-derived DNA with low frequency due to imprecise excision from the genome upon prophage induction. Prophage λ integrates between the gal (galactose metabolism) and bio (biotin metabolism) operons in the E. coli genome. In rare cases, λ excision is imprecise and either the gal or the bio operon is excised and packaged in the phage particle (Fig. 2a) . This packaging of adjacent E. coli host-derived DNA can lead to the transduction of the bio and the gal operons. Transduction of recipient cells can be temporary or permanent, depending on if the DNA gets recombined into the chromosome or remains as an extrachromosomal element, which is diluted out in the population during cell divisions .
Using the transductomics approach, we found that coverage of the E. coli genome with sequencing reads derived from purified λ phage particles is almost exclusively restricted to the λ phage integration site and two ~ 25 kbp regions on the left and right of the λ integration site (Fig. 2b, c). These flanking regions with read coverage represent the regions that are transduced by λ phage as indicated by the presence of the bio and the gal operons in these flanking regions (Fig. 2c). The coverage of the λ prophage region is roughly 10,000-fold greater than the coverage of the flanking transduced regions indicating that only a small number of phage particles actually carry transduced DNA and thus are specialized transducing particles.
Using the E. coli-prophage λ model, we show that specialized transduction by a prophage produces a unique read coverage pattern. Furthermore, analysis of the read coverage pattern of the transduced DNA region adjacent to the prophage DNA allows determination of both the size and content of the transduced host genome region (~ 50 kbp in total in case of λ), as well as estimation of the frequency with which transducing particles are produced (1:10,000 in case of λ). The number of transducing particles produced based on our data is roughly 100-fold higher than previously reported values for successful transduction of the gal operon by phage λ (1:1,000,000 successful transductions per λ particles) , which indicates that only a small fraction of λ carrying host DNA ultimately leads to successful transduction.
Generalized transduction of the Salmonella enterica serovar typhimurium LT2 genome by phage P22 and the E. coli genome by phage P1
We used two well-studied generalized transducing bacteriophages P22 and P1 to analyze the sequencing coverage patterns produced during generalized transducing events. In generalized transduction, nonspecific host chromosomal DNA is packaged into phage particles during lytic infection and can then be injected into a new host cell (Fig. 3a). The DNA can then recombine into the host chromosome by homologous recombination.
98.2% of the sequencing reads from purified P22 particles mapped to the P22 genome leaving 1.8% of reads that map to the S. enterica genome. The percentage of P22 particles mapping to the S. enterica genome corresponds to the reported percentage of 1.5% transducing P22 particles (i.e., carry host DNA) previously reported . The mapped P22-derived reads covered the S. enterica genome unevenly, while whole genome sequencing of S. enterica yielded even coverage (Fig. 3b). Regions of high or low P22 read coverage corresponded in 23 out of 28 previously reported transduced chromosomal markers  (Fig. 3b). Only one region at around 4 Mbp, for which high transduction frequencies had been reported, did not show high coverage (Fig. 3b), which might be due to differences in pac sites within this region between the S. enterica strain used in our study and the strain used in 1982.
The coverage of P22-derived reads showed a distinct pattern of peaks that rise vertically on one side and decline slowly over several 100 kbp increments on the other side. We speculate that the vertical edge of the peak corresponds to the location of the pac site at which the packaging of DNA into phage heads is initiated and that the slope of the peak indicates the range of processivity of the headful packaging mechanism (i.e., how many headfuls are packaged into particles before the packaging apparatus dissociates from the chromosome). This speculation is based on several facts: (1) the size of host DNA carried by transducing particles corresponds to the size of the P22 genome (~ 44 kbp) ; (2) the P22 genome is replicated by rolling circle replication, which produces long concatemers of P22 DNA. A specific sequence on the phage DNA (pac site) initiates the packaging of these concatemers into phage heads using a headful mechanism ; (3) the packaging of phage DNA continues sequentially along the P22 genome concatemer with a decreasing probability for each next headful to be encapsulated in a phage particle ; (4) there are five to six sequences on the S. enterica genome that are similar to the pac site, which leads to packaging of Salmonella DNA into P22 particles upon P22 infection, albeit with much lower frequency as compared to P22 DNA .
For E. coli phage P1, the majority of sequencing reads from purified P1 particles mapped to the P1 genome and only 4.5% of the reads mapped to the E. coli genome. The percentage of transducing P1 phages was previously reported to be 6% . We also observed that the P1-derived reads mapping to the E. coli genome covered the genome unevenly. However, the pattern was less pronounced as compared to P22 and S. enterica (Fig. 3c). This low unevenness in sequencing read coverage corresponds to previous data on transduction frequencies of chromosomal markers, which found a maximum transduction frequency across the E. coli genome of 10-fold .
Sequencing host DNA carried in generalized transducing phages reveals uneven read coverage patterns along the host genome indicative of transduction. These patterns vary in magnitude depending on the transducing phage and they can only be observed if read coverage is analyzed along long stretches of the host genome covering multiples of the length of the DNA carried by the transducing phage, e.g., in case of P22 44 kbp. Additionally, the patterns also provide an indication of the frequency with which different regions of the host genome are transduced, as well as the locations of the pac sites.
Hijacking of helper prophage by a phage-related chromosomal island in Enterococcus faecalis and lateral transduction by prophages
Certain chromosomal islands, including pathogenicity islands and integrative plasmids, are mobilized using helper phages [14, 34]. This is a form of molecular piracy in which structural proteins of the helper phage are hijacked by the chromosomal island and used as a vehicle for the transfer of the island to other cells. We used E. faecalis VE14089, which is a natural resident of the human intestine and causes opportunistic infections, to study the sequencing coverage patterns produced by chromosomal island transfer by way of a helper phage. E. faecalis VE14089 is host to a chromosomal island (EfCIV583) that uses structural proteins from a helper phage (φ1) for transfer [15, 34, 35] (Fig. 4a). E. faecalis VE14089 possesses five additional prophage-like elements (pp2 to pp6). Some of these prophages contribute to E. faecalis pathogenicity and confer an advantage during competition with other E. faecalis strains in the intestine [15, 36].
Read coverage differs widely between the different prophage like elements with the coverage of EfCIV583 exceeding the coverage of all other elements by almost an order of magnitude (Fig. 4b). This finding is in line with previous results that showed that EfCIV583 DNA is more abundant than all other prophage DNA in purified VLPs from E. faecalis V583 , an isogenic strain of VE14089. Interestingly, pp2, pp4, and pp6 did not yield coverage peaks in the VLP fraction, which confirms previous observations that these prophage elements are not excised under the conditions that we used for prophage induction .
For φ1, pp5 and EfCIV583 we see patterns (coverage slopes visible in the log-scale coverage plot) that indicate that not only the chromosomal island is transduced but also regions adjacent to these prophages and the chromosomal island. Based on the maximal coverage of the transduced regions versus the coverage of the prophage regions (Fig. 4b), we estimate the maximal frequencies of transduction to be 1:500 for φ1, 1:240 for pp5, and 1:43 for the left side of EfCIV583 and 1:3780 for the right side of EfCIV583. These relatively high transduction frequencies and the fact that transduced regions span several hundred kbp facing unidirectional from the integration site of the prophages and EfCIV583 suggest a lateral transduction mechanism as described by Chen et al. . For pp3, read coverage was overall much lower and no obvious transduction pattern was observed.
Our data also revealed that there are several additional regions in the E. faecalis VE14089 genome that had an elevated sequencing coverage in the purified phage sample suggesting that these regions encode additional elements that are transported in VLPs. These elements consist of IS-Elements that carry a transposase and surprisingly the three rRNA operons. For the rRNA operons, the coverage has a deep valley between the 16S and the 23S rRNA gene suggesting that a specific mechanism for rRNA gene transport is present or that the processed rRNAs were sequenced. We can currently think of four explanations for this intriguing pattern. First, potentially ribosomes are enriched alongside the VLPs in our VLP purification method. However, if this were the case we should have observed similar patterns in VLP fractions of other pure culture organisms, which we did not. Second, intact ribosomes are packaged by VLPs produced in E. faecalis. However, this leaves open the question of why the rRNA from these ribosomes was amenable to sequencing by the Illumina method used, which should not enable direct sequencing of RNA. Third, the E. faecalis genome contains a gene for a group II intron reverse transcriptase, which could potentially reverse transcribe rRNA into short DNA fragments that would be packaged into VLPs. This explanation is highly speculative, however, as the enzyme has not been experimentally characterized and it would require broad off-target activity to reversely transcribe a significant amount of rRNA. Fourth, genomic DNA with rRNA genes are packaged with high specificity into one or several types of VLPs from E. faecalis.
Our results show that the transduction of a chromosomal island by a prophage can produce a similar coverage pattern as an induced prophage, indicating that chromosomal islands are easy to detect based on read mapping coverage. However, they can only be distinguished from prophage by annotation of the genes and genomic regions and potentially based on their often times smaller size.
Transport of Bacillus subtilis genome by gene transfer agent-like element PBSX
We induced the gene transfer agent (GTA)-like element PBSX from the B. subtilis ATCC 6051 genome to study the sequencing coverage pattern produced by the supposedly randomized incorporation of fragments from the whole genome into GTA type VLPs. PBSX is a defective prophage that randomly packages 13 kbp DNA fragments of the B. subtilis genome in a GTA-like fashion (Fig. 4c) [10, 38, 39]. In contrast to other GTAs, it does not transfer the packaged DNA between cells but rather acts similar to a bacteriocin against B. subtilis cells that do not carry the PBSX gene cluster .
DNA sequencing reads derived from purified PBSX particles covered the B. subtilis genome unevenly with a maximum 30-fold difference between the lowest and highest covered regions (Fig. 4d). Reads from whole genome sequencing of B. subtilis covered the genome evenly slightly increasing toward the origin of replication, as expected . The genomic region containing PBSX had a lower read coverage in VLP particle-derived reads as compared to neighboring genomic regions. This is consistent with results from a previous study where it was found that a genetic marker integrated in the PBSX region was less frequently packaged into particles as compared to a marker in a neighboring region . Interestingly, the genomic region containing the prophage SPbeta, which gets excised upon mitomycin C treatment , did not show any higher or lower coverage in the VLP particle-derived sequencing reads as compared to neighboring genomic regions (Fig. 4d).
Our results show that packaging of host DNA by the GTA-like PBSX element of B. subtilis produces a distinct and non-random sequencing coverage pattern that bears similarities to the read coverage pattern produced by the generalized transducing phage P1 (Fig. 3c).
Detection limits of the approach
The patterns for different transduction modes have distinct characteristics that will impact sensitivity of detection and the false positive rate. For prophage induction and specialized transduction pattern detection, there are three potential challenges: (1) the length of the genome sequence fragment (contig) used for read mapping needs to be sufficiently long to encompass both the prophage genome, as well as a portion of the host genome; (2) potential assembly artifacts (chimeric contigs consisting of multiple source genomes) can lead to highly uneven read coverage that could look similar to an induced prophage pattern. In the case of our approach, this is mitigated by the fact that we map whole metagenome and VLP reads to the same contigs and thus we expect to obtain even read coverage for the whole metagenome read mapping, which is indicative of correct assembly; and (3) if read coverage is too low, patterns will not be sufficiently distinct. It can be expected that frequency of specialized transduction is specific to host species and prophages. Nevertheless, we tested the lower limit of read coverage levels needed for detection of prophage induction and specialized/lateral transduction by down sampling read numbers for the VLP reads from E. coli prophage λ and E. faecalis prophages (Figs. 2 and 4b) to achieve coverage levels similar to what we observed for our mouse case study below, which ranged from several tenfold to several thousand fold. For E. coli prophage λ, we found that at ~ 6000× maximum read coverage (5% of total reads), the specialized transduction pattern was still weakly visible, but disappeared at lower coverages, while the induction of the prophage itself was still identifiable at read coverages of 20× (0.01% of total reads) and less (Fig. S1a). For the E. faecalis prophages, specialized/lateral transduction patterns were still visible at ~ 500× coverage for the pp1 region and at ~ 150× for the pp5 region. Prophage induction was detectable at coverages well be low 40× (Fig. S1b). These results indicate that specialized and lateral transduction, as well as prophage induction, can sufficiently be detected with read coverages obtained in shotgun metagenomic sequencing of VLPs.
For generalized transduction and GTA-mediated DNA transfer pattern detection, the two main challenges are (1) potential generation of similar patterns by contamination of the ultra-purified VLPs with DNA from microbial cells, which can for example be addressed by comparing contig rank abundances between whole metagenome and VLP read coverage (see below in case study) and (2) difficulty to recognize the pattern on short contigs, because sloping can extend across 100s of kbp. To test if generalized transduction or GTA-like patterns can be detected on shorter contigs we used the P22, P1 and PBSX data to simulate how contig length impacts pattern visibility. For this, we looked at the coverage patterns of 200 kbp long stretches in the genome (Fig. S2). We found that detectability of generalized and GTA-like patterns in 200 kbp sequence stretches depended on where the 200 kbp stretch was located within the overall read coverage pattern. In some cases, distinguishable coverage sloping was observed (e.g., #2 in Fig. S2a and #2 in S2c); in other cases, coverage looked even or irregular (e.g., #4 in S2b and #4 in S2c). These results indicate that generalized transduction and GTA-mediated DNA transfer can be detected from contig lengths produced using short read shotgun metagenomics of microbiome samples; however, some DNA transfer events are likely missed if the longer contigs do not cover regions that show the characteristic coverage sloping associated with these transfer events.
Case study: high occurrence of transduction in the intestinal microbiome
We next assessed the power and application of our transductomics approach for detecting transduced DNA in VLPs from complex microbiomes. We sequenced the whole metagenome (~ 390 mio reads) and VLPs (~ 360 mio reads) from a fecal sample of one mouse to high coverage. The VLPs were ultra-purified using the multi-step procedure shown in Fig. 1, for which we previously showed that it efficiently removes DNA from microbial cells and the mouse present in fecal samples . We were able to assemble 2143 contigs > 40 kbp from the whole metagenome reads with the largest contig being 813 kbp (ENA accession for assembly: ERZ1273841). We discarded contigs < 40 kbp because detection of transduction patterns requires coverage analysis of a sufficiently large genomic region. We mapped the metagenomic and VLP reads to the contigs > 40 kbp to obtain the read coverage patterns. For complete metagenome, 44% of all reads mapped to the contigs > 40 kbp and for the VLPs 10% of all reads indicating that a large portion of DNA carried in VLPs is derived from prophages and microbial hosts. Of the 2143 contigs, 1957 showed a “standard” read coverage pattern (Fig. 5a, Suppl. Table S1), i.e., high even coverage of the contigs with metagenomic reads and low even or no coverage with VLP reads, indicating no mobilization of host DNA in VLPs. The remaining 186 contigs (8.6% of all contigs > 40 kbp) showed a read coverage pattern that indicates potential mobilization of DNA in VLPs (Fig. 5b-f, Suppl. Table S1).
To verify that the multi-step VLP ultra-purification procedure employed for this study efficiently removes DNA from microbial cells, ruling out potential microbial host DNA contamination, we further assessed read coverage patterns for the 186 contigs in comparison to all contigs. We ranked all 2143 contigs by their normalized coverage for both the whole metagenome and the purified VLP samples (i.e., average × fold read coverage/sum of average × fold read coverage for the sample) with the highest normalized coverage being assigned rank 1 (Table S1). The expectation is that contigs from which DNA is carried in VLPs have the same or lower rank for the VLP sample as compared to the whole metagenome sample, while the rank for contigs for which VLP reads are derived from microbial contamination should have a higher rank as compared to the whole metagenome reads, because randomly contaminating DNA would be depleted in the purified VLP sample. We found that 26 out of the 186 contigs with coverage patterns suggesting DNA mobilization had a normalized coverage based rank that was higher for VLP read coverage than for whole metagenome read coverage indicating that these 26 patterns are potentially due to contamination or alternatively due to very low efficiency of mobilization.
We classified all contigs taxonomically using CAT  (Suppl. Tables S2 and S3). The majority of contigs were classified as Bacteroidetes (all contigs: 805, transduction pattern contigs: 83), Firmicutes (all: 586, transduction pattern: 42), Proteobacteria (all: 89, transduction pattern: 3), or not classified at the phylum level (all: 527, transduction pattern: 34). The distribution of assigned contig taxonomy corresponds to what is expected for the microbiota of a healthy mouse . We found that with a few exceptions the relative abundance of contigs assigned to specific phyla was similar between the set of all contigs > 40 kbp and the subset of contigs with transduction patterns. The phyla that differed in relative contig abundance were Proteobacteria with less than half the relative abundance in the contigs with transduction patterns, Verrucomicrobia with 3.5× and Candidatus Saccharibacteria with 11.5× the contig abundance in the contigs with transduction patterns. Since members of Cand. Saccharibacteria have been shown to be extremely small (200 to 300 nm) , it is likely that they share similar properties with bacteriophages in terms of size and density and thus might get enriched in the VLP fraction. In fact, all transduction patterns of Cand. Saccharibacteria contigs were classified as “unknown” or “unknown, potentially a small bacterium” prior to knowing the taxonomic identity of the contigs.
We classified the type of DNA mobilization/transduction in the 186 contigs with a mobilization pattern based on the visual characteristics of the mobilized region in the VLP read coverage, as well as based on annotated genes within the mobilized region. For example, we classified mobilization patterns as prophage if the characteristic pattern showed high coverage with sharp edges on both sides (compare Fig. 2) and the presence of characteristic phage genes (e.g., capsid proteins) as an additional but not required criterion.
We observed 74 contigs that indicated induced prophages. Of these, 12 (16%) prophages showed indications of specialized transduction, i.e., read coverage above the base level of the contig in regions adjacent to the prophage (Fig. 5b and d). Additionally, we classified 8 patterns as potential prophages or chromosomal islands, as they showed the same pattern as other prophages, but we were unable to find recognizable phage genes in the annotations.
We found patterns of potential generalized transduction or GTA-carried DNA in 46 contigs; however, some patterns were observed for shorter contigs and could thus potentially be incorrect classifications (Fig. 5c). One of the contigs (NODE_5, classified as Bacteria) with a generalized transduction or GTA pattern additionally showed a sharp coverage drop in a ~ 15 kbp region only in the VLP reads (Fig. 5c). This region is flanked by a tRNA gene and carries one gene annotated as a potential virulence factor, internalin used by Listeria monocytogenes for host cell entry . This region might represent a chromosomal island that was excised from the bacterial chromosome prior to or during production of the unknown VLP and that did not get encapsulated in the VLP. Alternatively, similar strains might be present in the sample, but only some carry the chromosomal island and strains carrying the chromosomal island are less prone to producing the VLPs, e.g., by superinfection resistance provided by the chromosomal island against a generalized transducing phage.
We observed 9 patterns that showed strong differences between whole metagenome read coverage and VLP read coverage, but that did not correspond to any of the patterns we analyzed in our proof-of-principle work. However, based on gene annotations, we determined that these patterns likely represent retrotransposons or other transposable elements. For example, on contig NODE_1640 (classified as Bacteria by CAT), we observed high coverage with VLP reads on one part of the contig, which carries a gene annotated as a retrotransposon (Fig. 5e). Interestingly, the retrotransposon region is flanked by a ltrA gene which is encoded on bacterial group II intron and encodes maturase, an enzyme with reverse transcriptase and endonuclease activity . Surprisingly, the region containing the ltrA gene had above average coverage in the whole metagenome reads, but no coverage in the VLP reads. This suggests that the intron actively reverse splices into expressed RNA with subsequent formation of cDNA  leading to increased copy number of this genomic region. As another example, on contig NODE_1223 (classified as Bacteria by CAT) a region containing a transposase gene is strongly overrepresented in the VLP reads suggesting that this region is a transposable element that is packaged into a VLP (Fig. 5e).
Finally, we determined that two patterns are likely lytic phages and 47 patterns are classified as “unknown” transduced DNA, as the coverage pattern is uneven indicating transport in VLPs but we could not determine the type of transport. To provide an example, in contig NODE_646 (classified as Clostridiales by CAT), we observed a potential prophage pattern in which we found some of the main phage relevant genes such as major capsid protein; however, within the prophage pattern, we observed high coverage spikes for which we currently have no good explanation (Fig. 5f).
Conclusions and outlook
The transductomics approach that we developed should be applicable to a broad range of environments ranging from host-associated microbiomes to soils and aqueous environments. For some environments such as open ocean water samples, the approach would need only minor modifications. For example, concentration of VLPs by tangential flow filtration prior to density gradient centrifugation using well established protocols . Thus, this approach will allow addressing key questions about microbial evolution via HGT in a diversity of microbial communities, including what kind of genes and with what frequency are carried by VLPs. Among these questions, one of the most pressing ones is what the role of transducing particles is in the transfer of antibiotic resistance genes, which is a topic of current debate [48,49,50,51]. Apart from its application in studying transduction in microbial communities, this approach can also be used to increase our understanding of the molecular mechanisms of different transduction mechanisms by careful analysis of read coverage data from pure cultures that shows exact transduction frequencies of each genomic location without tedious analysis of multiple genomic markers. Mechanisms that can be analyzed include, for example, the identification of pac sites in generalized transducers, the size range of transduced genomic loci in specialized transducers , and the analysis of how random DNA packaging by GTAs really is .
One of the major surprises for us when analyzing the mouse intestinal transductome data was that around one quarter of the transduction patterns that we identified are unknown. These patterns showed even coverage in the whole metagenome reads and strong uneven coverage in the VLP reads (e.g., Fig. 4e); however, we were unable to associate them clearly with any of transduction modes that we have investigated with pure cultures. We foresee two types of future studies to characterize the nature of the transducing particles that lead to these unknown patterns and to exclude that they are some kind of artifact. First, read coverage patterns of newly discovered modes of transduction have to be analyzed with the “transductomics” approach to correlate their patterns to patterns observed in microbiomes and microbial communities. While we investigated the transduction patterns associated with both major known transduction pathways, as well as more recently discovered transduction pathways, novel modes of transduction are continuously discovered. These novel transduction modes that need to be characterized with our approach include new types of GTAs , lateral transduction  and DNA transfer in outer membrane vesicles [52, 53]. Second, approaches that allow linking specific transduced DNA sequences to the identity of transducing particles in microbial community samples can be developed. We envision, for example, that high resolution filtration and density gradient based separation of individual VLPs will allow linking the transduced DNA (by sequencing) to the identity of the transducing VLPs using proteomics to identify VLP proteins. Using and developing these approaches further will allow us to increase the range of transduction modes that can be detected in microbial communities, as well as potentially reveal currently unknown types of transduction that are not known from pure culture studies yet.
We see several pathways for improving the sensitivity, accuracy, and throughput of the transductomics approach in the future. Currently, our ability to detect generalized transduction patterns is limited by the fact that detection of these patterns requires long stretches of the microbial host genome to be assembled. Our P22 and P1 data shows these patterns stretch across genomic regions > 500 kbp. Additionally, high sequencing coverage is needed for the detection of these patterns. Assembly of long contigs in metagenomes of high-diversity communities is currently hampered by the relatively short read lengths of sequencers that allow for high coverage. We expect that increasing read numbers of long-read sequencing technologies such as PacBio and Oxford Nanopore in the future will allow us to sequence complex microbiomes to sufficient depth for the assembly of long metagenomic contigs. A combination of long-read sequencing for the whole community metagenomes in combination with a short-read, high-coverage approach for the VLP fraction will in the future provide more sensitive and accurate detection of generalized transduction patterns. In addition to improvements in the realm of long-read sequencing, we expect the development of computational tools for the automatic or semi-automatic detection of transduction patterns in read coverage data from paired whole metagenome and VLP metagenome sequencing. There is a large number of possible parameters that could be used to train a machine learning algorithm to detect transduction patterns. These parameters include differences between average read coverage and maximal read coverage for VLP reads (Table S1) and the comparison of contig rank abundance based on coverage, which we used to cross check transduction patterns for signatures of microbial DNA contamination. Such computational tools will enable the high-throughput detection of transduction patterns in many samples, which is currently limited by the need for visual inspection of patterns.
Materials and methods
In vitro bacteriophage propagation and induction of transducing prophages and other elements
E. coli KL740 was inoculated into 300 ml of LB and grown to on OD600 of 0.7 at 28° C with aeration. The culture flask was transferred to a 42° C water bath for 10 min and then incubated at 42° C for 30 min with shaking. The temperature was reduced to 28° C and cell lysis was allowed to proceed for 2 h. The remaining cells and debris were removed by centrifugation at 2750 × g for 10 min and the phage containing culture fluid was filtered through a 0.45 μm membrane.
The data set used to analyze generalized transduction by Salmonella phage P22 was taken from a previous study assessing methods for phage particle purification from intestinal contents . For a detailed description of P22 propagation and purification, please refer to our previous publication.
Lyophilized phage P1 was purchased from ATCC and resuspended in 1 ml of Lennox broth (LB) at room temperature (RT). Two hundred microliters of the phage suspension was added to 10 ml of mid logarithmic phase (OD600 ~0.5) E. coli ATCC 25922 and incubated for 3 h at 37° C with shaking. The bacteria were pelleted at 2750 × g for 10 min and the culture fluid was filtered through a 0.45 μm syringe filter. One hundred microliters of stationary phase E. coli ATCC 25922 was distributed to 15 separate tubes each containing 200 μl of the P1 culture filtrate. The bacteria/phage mixtures were immediately added to molten LB top agar (0.5% agar), poured over LB agar (1.5% agar) plates, and incubated overnight (O/N) at 37° C. 2.5 ml of SM-plus buffer (100 mM NaCl, 50 mM Tris·HCl, 8 mM MgSO4, 5 mM CaCl2·6H2O, pH 7.4) was added to the surface each plate and the top agar was scraped off and pooled. The phages were eluted from the top agar by rotation for 1 h at RT. The top agar suspension was centrifuged at 2750 × g for 10 min, the supernatant was collected, and the top agar was washed once with ~ 30 ml of SM-plus and incubated at RT for an additional 1 h. Following the wash step centrifugation was repeated, and the resulting supernatant was collected. The phage containing supernatants were combined.
To induce the prophage-like element PBSX from the B. subtilis ATCC 6051 genome, a 100 ml culture of B. subtilis was grown in LB at 37° C with shaking to an OD600 of ~0.5. Mitomycin C was added at a final concentration of 0.5 μg/ml and the culture was incubated at 37° C for 10 min. The culture was centrifuged at 2750 × g for 10 min and the pellet was washed with 50 ml of fresh LB and centrifuged a second time. The cell pellet was resuspended in 100 ml of LB and grown for an additional 3 h at 37° C with shaking. The cells and debris were removed by centrifugation and the phage containing culture fluid was filtered through a 0.45 μm membrane.
E. faecalis strain VE14089, a derivative of E. faecalis V583 that has been cured of its three endogenous plasmids , was subcultured to an OD600 of 0.025 in 1 L of pre warmed brain heart infusion broth (BHI) and grown statically at 37° C to an OD600 of 0.5. To induce excision of integrated prophages, ciprofloxacin was added to the culture at a final concentration of 2 μg/ml and the bacteria were grown for an additional 4 h at 37° C. The bacterial cells and debris were centrifuged at 2750 × g for 10 min and the culture fluid was filtered through a 0.45 μm membrane.
Purification of phage particles from culture fluid
All phage containing culture fluid was treated with 10 U of DNase and 2.5 U of RNase for 1 h at RT. 1 M solid NaCl and 10 % wt/vol polyethylene glycol (PEG) 8000 was added and the phages were precipitated O/N on ice at 4° C. The precipitated phages were resuspended in 2 ml of SM-plus and loaded directly onto CsCl step gradients (1.35, 1.5, and 1.7 g/ml fractions) and centrifuged for 16 h at 83,000 × g. The phage bands were extracted from the CsCl gradients using a 23-gauge needle and syringe, brought up to 4 ml with SM-plus buffer and loaded onto a 10,000 Da molecular weight cutoff Amicon centrifugal filter (EMD Millipore) to remove excess CsCl. The phages were washed 3 times with ~4 ml of SM-plus and then stored at 4° C.
Isolation of phage and host bacterial DNA from pure cultures
Following CsCl purification of phages and phage-like elements, DNA was isolated by adding 0.5% SDS, 20 mM EDTA (pH = 8), and 50 μg/ml Proteinase K (New England Biolabs) and incubating at 56° C for 1 h. Samples were cooled to RT and extracted with an equal volume of phenol to chloroform to isoamyl alcohol. The samples were centrifuged at 12,000 × g for 1 min, and the aqueous phase containing the DNA was extracted with an equal volume of chloroform. Following centrifugation at ~16,000×g for 1 min, 0.3 M NaOAc (pH = 7) was added followed by an equal volume of 100% isopropanol to precipitate the DNA. The DNA was pelleted at 12,000 × g for 30 min and washed once with 500 μl of 70% ethanol. The samples were decanted and the pellets were air dried for 10 min and resuspended in 100 μl of sterile water.
For the isolation of bacterial genomic DNA, we used the Gentra Puregene Yeast/Bacteria Kit (Qiagen) according to the manufacturer’s instructions.
Isolation and purification of bacteria and VLPs from mouse fecal pellets for metagenomic sequencing
The entire colon contents of one male C57BL6/J mouse were added to 1.2 ml of SM-plus buffer and homogenized manually with the handle of a sterile disposable inoculating loop. After homogenization, the sample was brought up to 2 ml with SM-plus. One third of the sample volume was added to a fresh tube containing 100 mM EDTA and set aside on ice. This represented the unprocessed whole metagenome sample. The remaining two thirds of the sample volume were used to isolate VLPs.
VLPs from the homogenized feces were ultra-purified as described previously . Briefly, the sample was centrifuged at 2500 × g for 5 min; the supernatant was transferred to a clean tube and centrifuged a second time at 5000 × g to pellet any residual bacteria and debris. The supernatant was transferred to a sterile 1 ml syringe and filtered through a 0.45 μm syringe filter. The clarified supernatant was treated with 100 U of DNase and 15 U of RNase for 1 h at 37° C. The sample was loaded onto a CsCl step gradient (1.35, 1.5, and 1.7 g/ml fractions) and centrifuged for 16 h at 83,000 × g. The VLPs residing at the interface of the 1.35 and 1.5 g/ml fractions were collected (~2 ml), and the CsCl was removed by centrifugal filtration as described above. The purified VLPs were disrupted by the addition of 50 μg/ml proteinase K and 0.5% sodium dodecyl sulfate (SDS) at 56° C for 1 h. The samples were cooled to room temperature, and total DNA was extracted by the addition of an equal volume of phenol to chloroform to isoamyl alcohol. The organic phase was separated by centrifugation at 12,000 × g for 2 min, and the aqueous phase was extracted with an equal volume of chloroform. The DNA was precipitated by the addition of 0.3 M NaOAc, pH 7, and an equal volume of isopropanol. The DNA pellet was washed once with ice cold 70% ethanol and resuspended in 100 μl of sterile water. The DNA was further cleaned on a MinElute spin column (Qiagen) and eluted into 12 μl of elution buffer (Qiagen).
To purify total metagenomic DNA, unclarified fecal homogenate was treated with 5 mg/ml lysozyme for 30 min at 37° C. The sample was transferred to 2 ml Lysing Matrix B tubes (MP Biomedical) and bead beat in a Bullet Blender BBX24B (Next Advance) at top speed for 1 min followed by placing on ice for 2 min. This was repeated a total of 4 times. The samples were centrifuged at 12,000 × g for 1 min, and the DNA from the supernatant was extracted, precipitated, and purified as described above.
The concentration of purified microbial DNA was determined using a Qubit 3.0 fluorometer (Thermo-Fisher). Prior to library preparation total microbial DNA was sheared using a Covaris S2 sonicator with a duty cycle of 10%, intensity setting of 5.0, and a duration of 2 × 60 s at 4° C. Sequencing libraries were generated using the KAPA HTP library preparation kit KR0426 – v3.13 (KAPA Biosystems) with Illumina TruSeq ligation adapters. Library quality was determined using a 2100 Bioanalyzer system (Agilent). Libraries were size selected and purified in the range of 300–900 bp fragments and subjected to Illumia deep sequencing. For DNA sequencing of pure phage cultures and the E. coli KL740 genome, we used an Illumina NextSeq 500 desktop sequencer. Illumina Hiseq 2500 v3 Sequencing System in rapid run mode was used to sequence the metagenomes and viromes from the feces of the C57BL6/J mouse. All sequencing was performed in paired end mode acquiring 150 bp reads. Per fragment end, the following number of reads were obtained: C57BL/6 mouse feces—76 M reads for the whole metagenome and 97 M reads for the virome, 45 M reads for phage P1, 21 M reads for lambda phage and 27 M reads for the E. coli KL740 genome, 29 M reads for phage PBSX and 28 M reads for the enterococcal prophages. For the C57BL/6 mouse microbiome, we sequenced an additional 75 bp single-end read run to increase coverage. We obtained 313 M 75 bp reads for the whole metagenome and 262 M 75 bp reads for the virome. All DNA sequencing was performed by the Eugene McDermott Center for Human Growth and Development Next Generation Sequencing Core Facility at the University of Texas Southwestern.
Assembly of mouse fecal metagenome
Read decontamination and trimming of the mouse metagenome 75 and 150 bp reads were performed using the BBMap short read aligner (v. 36.19)  as previously described . Briefly, for decontamination, raw reads were mapped to the internal Illumina control phiX174 (J02482.1), the mouse (mm10) and human (hg38) reference genomes using the bbsplit algorithm with default settings. The resulting unmapped reads were adapter trimmed, and low-quality reads and reads of insufficient length were removed using the bbduk algorithm with the following parameters: ktrim=lr, k=20, mink=4, minlength=20, qtrim=f. The reads were assembled using SPAdes version 3.6.1  with the following parameters: --only-assembler -k 21,33,55,77,99. Assembled contigs < 40 kbp were discarded. The assembly resulted in 2143 contigs > 40 kbp.
Taxonomic classification and annotation of metagenomic contigs
The 2143 contigs > 40 kbp from the assembly of the mouse fecal metagenome were taxonomically classified using the Contig Annotation Tool (CAT)  (version 2019-07-19). Genes were predicted and annotated using the automated PROKKA pipeline (v1.11) .
Read mapping and read coverage visualization
The whole (meta)genome and purified VLP read sets were mapped onto the corresponding reference genomes of pure culture organisms or the mouse fecal metagenome contigs using BBmap  with the following parameters: ambiguous=random qtrim=lr minid=0.97. For testing detection limits, reads were randomly subsampled using the reformat.sh tool, which is part of the BBmap tools, prior to read mapping. The generated read mapping files (.bam) were sorted and indexed using SAMtools (v. 1.7) . Integrative Genomics Viewer (IGV, v. 2.3.67) tools were used to generate tiled data files (.tdf) from the read mapping (.bam) files for data compression and faster access in IGV using the following parameters: count command, zoom levels: 9, using the mean, window size: 25 or 100 . Read coverage patterns were displayed and visually assessed in IGV using a linear or if needed log scale.
Availability of data and materials
All sequencing read data generated for this study is available from the European Nucleotide Archive (ENA) through study PRJEB33536 (https://www.ebi.ac.uk/ena/data/view/PRJEB33536). This data includes the reads for the mouse whole metagenomes and VLP fraction, E. faecalis VLPs, B. subtilis VLPs, E. coli phage P1, E. coli phage λ, the whole genome sequencing of E. coli KL740 (E. coli with lambda phage integrated) and the contigs > 40 kbp from the mouse whole metagenome assembly (contig file accession number ERZ1273841).
In addition to the de novo generated data we used existing genome assemblies and sequencing read sets for individual bacteria including Bacillus subtilis subsp. subtilis str. 168 complete genome from NCBI RefSeq (NC_000964.3), B. subtilis 168 genome sequencing read set from the ENA (Study: PRJDB1076, Sample: SAMD00008600), Enterococcus faecalis V583 complete genome from NCBI RefSeq (NC_004668.1), E. faecalis V583 sequencing read set from the ENA (Study: PRJEB13005, Sample: ERS1085927), Escherichia coli K12 complete genome from NCBI RefSeq (NC_000913.3), E. coli NCM3722 (E. coli K12 with Lambda phage integrated) complete genome sequence from NCBI GenBank (CP011495.1), phage P1 complete genome sequence from NCBI RefSeq (NC_005856.1), E. coli K12 genome sequencing read set from the ENA (Study: PRJNA251794, Sample: SAMN02840692), Salmonella enterica subsp. enterica serovar typhimurium str. LT2 complete genome sequence from NCBI RefSeq (NC_003197.1), S. typhimurium LT2 genome sequencing read set from ENA (Study: PRJNA203445, Sample: SAMN02367645), and a read set of CsCl density gradient purified P22 phage (Study: PRJEB6941, Sample: SAME2690949) .
Zhaxybayeva O, Doolittle WF. Lateral gene transfer. Curr Biol. 2011;21:R242–6.
Soucy SM, Huang J, Gogarten JP. Horizontal gene transfer: building the web of life. Nat Rev Genet. 2015;16:472–82.
Smillie CS, et al. Ecology drives a global network of gene exchange connecting the human microbiome. Nature. 2011;480:241–4.
A. Oladeinde, et al., Horizontal gene transfer and acquired antibiotic resistance in Salmonella enterica Serovar Heidelberg following in vitro incubation in broiler ceca. Appl. Environ. Microbiol. 85 (2019).
Borgeaud S, Metzger LC, Scrignari T, Blokesch M. The type VI secretion system of vibrio cholerae fosters horizontal gene transfer. Science. 2015;347:63–7.
Chen J, Novick RP. Phage-mediated intergeneric transfer of toxin genes. Science. 2009;323:139–41.
Hehemann J-H, et al. Transfer of carbohydrate-active enzymes from marine bacteria to Japanese gut microbiota. Nature. 2010;464:908–12.
Mongodin EF, et al. The genome of Salinibacter ruber: convergence and gene exchange among hyperhalophilic bacteria and archaea. Proc Natl Acad Sci. 2005;102:18147–52.
Popa O, Landan G, Dagan T. Phylogenomic networks reveal limited phylogenetic range of lateral gene transfer by transduction. ISME J. 2017;11:543–54.
Lang AS, Zhaxybayeva O, Beatty JT. Gene transfer agents: phage-like elements of genetic exchange. Nat Rev Microbiol. 2012;10:472–82.
Chiang YN, Penadés JR, Chen J. Genetic transduction by phages and chromosomal islands: the new and noncanonical. PLoS Pathog. 2019;15:e1007878.
Chen J, et al. Genome hypermobility by lateral transduction. Science. 2018;362:207–12.
A. Thierauf, G. Perez, and S. Maloy, “Generalized transduction” in Bacteriophages: Methods and Protocols, Volume 1: Isolation, Characterization, and Interactions, Methods in Molecular BiologyTM., M. R. J. Clokie, A. M. Kropinski, Eds. (Humana Press, 2009), pp. 267–286.
Christie GE, Dokland T. Pirates of the Caudovirales. Virology. 2012;434:210–21.
Matos RC, et al. Enterococcus faecalis prophage dynamics and contributions to pathogenic traits. PLoS Genet. 2013;9:e1003539.
Jiang SC, Paul JH. Gene transfer by transduction in the marine environment. Appl Environ Microbiol. 1998;64:2780–7.
Kenzaka T, Tani K, Nasu M. High-frequency phage-mediated gene transfer in freshwater environments determined at single-cell level. ISME J. 2010;4:648–59.
Sander M, Schmieger H. Method for host-independent detection of generalized transducing bacteriophages in natural habitats. Appl Environ Microbiol. 2001;67:1490–3.
Tomasch J, et al. Packaging of Dinoroseobacter shibae DNA into gene transfer agent particles is not random. Genome Biol Evol. 2018;10:359–69.
Pourcel C, Midoux C, Hauck Y, Vergnaud G, Latino L. Large preferred region for packaging of bacterial DNA by phiC725A, a novel Pseudomonas aeruginosa F116-like bacteriophage. PLoS One. 2017;12:e0169684.
Garneau JR, Depardieu F, Fortier L-C, Bikard D, Monot M. PhageTerm: a tool for fast and accurate determination of phage termini and packaging mechanism using next-generation sequencing data. Sci Rep. 2017;7:1–10.
Reyes A, Wu M, McNulty NP, Rohwer FL, Gordon JI. Gnotobiotic mouse model of phage–bacterial host dynamics in the human gut. Proc Natl Acad Sci U S A. 2013;110:20236–41.
Thurber RV, Haynes M, Breitbart M, Wegley L, Rohwer F. Laboratory procedures to generate viral metagenomes. Nat Protoc. 2009;4:470–83.
Kleiner M, Hooper LV, Duerkop BA. Evaluation of methods to purify virus-like particles for metagenomic sequencing of intestinal viromes. BMC Genomics. 2015;16:7.
B. Bushnell, BBMap. SourceForge (December 29, 2019).
Thorvaldsdóttir H, Robinson JT, Mesirov JP. Integrative genomics viewer (IGV): high-performance genomics data visualization and exploration. Brief Bioinform. 2013;14:178–92.
Gottesman ME, Weisberg RA. Little lambda, who made thee? Microbiol Mol Biol Rev. 2004;68:796–813.
Morse ML, Lederberg EM, Lederberg J. Transduction in Escherichia coli K-12. Genetics. 1956;41:142–56.
Ebel-Tsipis J, Botstein D, Fox MS. Generalized transduction by phage P22 in salmonella typhimurium: I. Molecular origin of transducing DNA. J Mol Biol. 1972;71:433–48.
Schmieger H. Packaging signals for phage P22 on the chromosome of salmonella typhimurium. Mol Gen Genet MGG. 1982;187:516–8.
Casjens S, Hayden M. Analysis in vivo of bacteriophage P22 headful nuclease. J Mol Biol. 1988;199:467–74.
Hanks MC, Newman B, Oliver IR, Masters M. Packaging of transducing DNA by bacteriophage P1. Mol Gen Genet MGG. 1988;214:523–32.
M. Masters, “Generalized transduction” in Escherichia Coli and Salmonella: Cellular and Molecular Biology, (American Society for Microbiology, 1996), pp. 2421–441.
Penadés JR, Christie GE. The phage-inducible chromosomal islands: a family of highly evolved molecular parasites. Annu Rev Virol. 2015;2(181–201).
Martínez-Rubio R, et al. Phage-inducible islands in the gram-positive cocci. ISME J. 2017;11:1029–42.
Duerkop BA, Clements CV, Rollins D, Rodrigues JLM, Hooper LV. A composite bacteriophage alters colonization by an intestinal commensal bacterium. Proc Natl Acad Sci. 2012;109:17621–6.
Kunst F, et al. The complete genome sequence of the gram-positive bacterium Bacillus subtilis. Nature. 1997;390:249–56.
Wood HE, Dawson MT, Devine KM, McConnell DJ. Characterization of PBSX, a defective prophage of Bacillus subtilis. J Bacteriol. 1990;172:2667.
Canchaya C, Fournous G, Chibani-Chennoufi S, Dillmann M-L, Brüssow H. Phage as agents of lateral gene transfer. Curr Opin Microbiol. 2003;6:417–24.
Korem T, et al. Growth dynamics of gut microbiota in health and disease inferred from single metagenomic samples. Science. 2015;349:1101.
Anderson LM, Bott KF. DNA packaging by the Bacillus subtilis defective bacteriophage PBSX. J Virol. 1985;54:773.
Abe K, et al. Developmentally-regulated excision of the SPβ prophage reconstitutes a gene required for spore envelope maturation in Bacillus subtilis. PLoS Genet. 2014;10:e1004636.
von Meijenfeldt FAB, Arkhipova K, Cambuy DD, Coutinho FH, Dutilh BE. Robust taxonomic classification of uncharted microbial sequences and bins with CAT and BAT. Genome Biol. 2019;20:217.
Ley RE, et al. Obesity alters gut microbial ecology. Proc Natl Acad Sci. 2005;102:11070–5.
He X, et al. Cultivation of a human-associated TM7 phylotype reveals a reduced genome and epibiotic parasitic lifestyle. Proc Natl Acad Sci. 2015;112:244–9.
M. Bonazzi, M. Lecuit, P. Cossart, Listeria monocytogenes internalin and E-cadherin: from bench to bedside. Cold Spring Harb. Perspect. Biol. 1 (2009).
Cousineau B, Lawrence S, Smith D, Belfort M. Retrotransposition of a bacterial group II intron. Nature. 2000;404:1018–21.
Haaber J, et al. Bacterial viruses enable their host to acquire antibiotic resistance genes from neighbouring cells. Nat Commun. 2016;7:1–8.
Enault F, et al. Phages rarely encode antibiotic resistance genes: a cautionary tale for virome analyses. ISME J. 2017;11:237–47.
Modi SR, Lee HH, Spina CS, Collins JJ. Antibiotic treatment expands the resistance reservoir and ecological network of the phage metagenome. Nature. 2013;499:219–22.
Calero-Cáceres W, Ye M, Balcázar JL. Bacteriophages as environmental reservoirs of antibiotic resistance. Trends Microbiol. 2019;27:570–7.
Bitto NJ, et al. Bacterial membrane vesicles transport their DNA cargo into host cells. Sci Rep. 2017;7:1–11.
Fulsundar S, et al. Gene transfer potential of outer membrane vesicles of Acinetobacter baylyi and effects of stress on vesiculation. Appl Environ Microbiol. 2014;80:3469–83.
Duerkop BA, et al. Murine colitis reveals a disease-associated bacteriophage community. Nat Microbiol. 2018;3:1023–31.
Bankevich A, et al. SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing. J Comput Biol. 2012;19:455–77.
Seemann T. Prokka: rapid prokaryotic genome annotation. Bioinformatics. 2014;30:2068–9.
Li H, et al. The sequence alignment/map format and SAMtools. Bioinformatics. 2009;25:2078–9.
We would like to thank Kelly Ruhn for assistance with animals and Vanessa Schmid and Rachel Bruce of the University of Texas Southwestern Medical Center’s Next Generation Sequencing Core for assistance with Illumina library construction and sequencing support, and Alejandro Reyes Muñoz for helpful comments on a previous version of the manuscript, and two anonymous reviewers for their feedback.
This work was supported in part by the USDA National Institute of Food and Agriculture Hatch project 1014212 (M.K.), the Foundation for Food and Agriculture Research Grant ID: 593607 (M.K.), the NC State Chancellor’s Faculty Excellence Program Cluster on Microbiomes and Complex Microbial Communities (M.K.), National Institutes of Health Grants R01AI141479 (B.A.D.), K01DK102436 (B.A.D), and R01DK070855 (L.V.H.), and the Howard Hughes Medical Institute (L.V.H.). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Ethics approval and consent to participate
The Institutional Animal Care and Use Committee of University of Texas Southwestern Medical Center approved all animal protocols.
Consent for publication
The authors declare no competing interests.
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
About this article
Cite this article
Kleiner, M., Bushnell, B., Sanderson, K.E. et al. Transductomics: sequencing-based detection and analysis of transduced DNA in pure cultures and microbial communities. Microbiome 8, 158 (2020). https://doi.org/10.1186/s40168-020-00935-5