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Fig. 5 | Microbiome

Fig. 5

From: Transductomics: sequencing-based detection and analysis of transduced DNA in pure cultures and microbial communities

Fig. 5

Example of transduction patterns in the mouse intestinal microbiome. The taxonomic classification for each contig specified in parentheses after each contig name is the lowest taxonomic level successfully classified by CAT [43] (Suppl. Table S2). The complete metagenome reads and the purified VLP reads were mapped to the same exact set of contigs assembled from the complete metagenome reads. The read coverage pattern of the complete metagenome reads provides evidence for the correct assembly of the contigs and allows to distinguish potential transduction-derived VLP read coverage patterns from VLP coverage patterns due to contamination with microbial DNA. With the exception of a, all shown contigs have the same or a lower abundance rank for VLP read coverage as compared to complete metagenome read coverage indicating that their overall read coverage was enriched in the VLP samples. Read coverage due to VLP sample contamination with cellular DNA is expected to result in a higher abundance rank for VLP read coverage, as compared to complete metagenome read coverage

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