Uncovering the trimethylamine-producing bacteria of the human gut microbiota
© The Author(s). 2017
Received: 18 October 2016
Accepted: 25 April 2017
Published: 15 May 2017
Trimethylamine (TMA), produced by the gut microbiota from dietary quaternary amines (mainly choline and carnitine), is associated with atherosclerosis and severe cardiovascular disease. Currently, little information on the composition of TMA producers in the gut is available due to their low abundance and the requirement of specific functional-based detection methods as many taxa show disparate abilities to produce that compound.
In order to examine the TMA-forming potential of microbial communities, we established databases for the key genes of the main TMA-synthesis pathways, encoding choline TMA-lyase (cutC) and carnitine oxygenase (cntA), using a multi-level screening approach on 67,134 genomes revealing 1107 and 6738 candidates to exhibit cutC and cntA, respectively. Gene-targeted assays enumerating the TMA-producing community by quantitative PCR and characterizing its composition via Illumina sequencing were developed and applied on human fecal samples (n = 50) where all samples contained potential TMA producers (cutC was detected in all individuals, whereas only 26% harbored cntA) constituting, however, only a minor part of the total community (below 1% in most samples). Obtained cutC amplicons were associated with various taxa, in particular with Clostridium XIVa strains and Eubacterium sp. strain AB3007, though a bulk of sequences displayed low nucleotide identities to references (average 86% ± 7%) indicating that key human TMA producers are yet to be isolated. Co-occurrence analysis revealed specific groups governing the community structure of cutC-exhibiting taxa across samples. CntA amplicons displayed high identities (~99%) to Gammaproteobacteria-derived references, primarily from Escherichia coli. Metagenomic analysis of samples provided by the Human Microbiome Project (n = 154) confirmed the abundance patterns as well as overall taxonomic compositions obtained with our assays, though at much lower resolution, whereas 16S ribosomal RNA gene sequence analysis could not adequately uncover the TMA-producing potential.
In this study, we developed a diagnostic framework that enabled the quantification and comprehensive characterization of the TMA-producing potential in human fecal samples. The key players were identified, and together with predictions on their environmental niches using functional genomics on most closely related reference strains, we provide crucial information for the development of specific treatment strategies to restrain TMA producers and limit their proliferation.
The human body hosts a myriad of bacteria that play important roles in host health and disease. It is becoming evident that besides particular pathogens, whole microbial consortia are involved in the development of certain diseases. This is exemplified by trimethylamine (TMA), a microbial metabolite that is produced by various taxa of the gut microbiota from dietary quaternary amines, mainly choline and carnitine. TMA is absorbed via the intestinal epithelium and further oxidized to trimethylamine N-oxide (TMAO), which was associated with atherosclerosis and severe cardiovascular disease in several independent studies [1–4]. A mechanistic model has been formulated proposing that TMAO promotes atherogenesis through the formation of foam cells (lipid-laden macrophages) and a reduction of the reverse cholesterol transport from the atherosclerotic plaque .
Two major TMA-synthesis pathways have been described with a specific glycyl radical enzyme, the choline TMA-lyase (CutC) and its activator CutD , that takes choline as a substrate and a two-component Rieske-type oxygenase/reductase (CntA/B) , which acts on carnitine and its derivative gamma-butyrobetaine, as the key enzymes. An additional enzyme complex termed YeaW/X, which shows close sequence similarity to CntA/B, was recently proposed as the key component of a third major pathway . Campo and colleagues have shown that the so-called choline utilization cluster, which includes cutC/D among other genes involved in anaerobic choline metabolism, is widely, but discontinuously, distributed across various taxa belonging to Firmicutes, Actinobacteria, and Proteobacteria . The authors biochemically verified TMA synthesis from choline for a multitude of the revealed taxa and demonstrated that both cutC and cutD are required for the TMA-generating cleavage reaction. Another recent study  demonstrated that colonization of the mouse gut with a specific consortium of CutC-encoding human isolates results in TMA synthesis and subsequent TMAO accumulation in the serum of animals where even minute concentrations of TMA producers (0.15% of the total community) were sufficient for substantial TMA production from choline. Genes encoding CntA/B and YeaX/Y, respectively, have been described in several taxa from the Gamma- and Betaproteobacteria as well as from a few Firmicutes . Functionality has been shown for Acinetobacter baumannii and Escherichia coli where both genes cntA/yeaX and cntB/yeaW are required for the formation of TMA.
Despite this multitude of knowledge on TMA-producing bacteria and their importance for human health, little information on their abundance and taxonomic composition in vivo is available. So far, only one study estimates the overall potential of intestinal communities to produce TMA , and specific structural insights into this functional community are largely lacking. This can partly be explained by the low abundance of TMA producers and the requirement of specific methods for their quantification, as phylogenetic markers are expected to be poor predictors for that function, at least for the broadly polyphyletic bacteria exhibiting cutC/D . Thus, the aim of this study was to build a comprehensive framework in order to quantify the TMA-production potential of intestinal communities and to gain detailed compositional insights into this important functional group. To this end, comprehensive databases for the key genes of all major pathways were constructed and gene-targeted assays were designed for quantitative PCR (qPCR) coupled to sequencing of PCR products on the Illumina MiSeq platform. The developed assays were used to characterize the TMA-producing communities in the fecal samples of 50 individuals.
Establishing databases for cutC and cntA
Since any gene-based investigations depend on reference sequences, the entire diversity spectrum of the key genes from major pathways involved in the formation of TMA was identified. Comprehensive databases for cutC and cntA were established applying a multi-level screening approach on 67,134 genomes provided by the Pathosystems Resource Integration Center (PATRIC). CntA and yeaW show high similarity, and sequences from both genes were included in our reference set used to construct a single hidden Markov model (HMM) for this gene group. All genes comprised in this database will be referred to as cntA throughout the study (see also the “Discussion” section).
Putative cntA genes were identified in 6738 genomes (491 dereplicated protein sequences) where the majority was observed in Proteobacteria, especially Gammaproteobacteria (mostly derived from Escherichia and Acinetobacter), and some entries belonging to Betaproteobacteria and Epsilonproteobacteria (Fig. 1b, Additional file 1B, Additional file 2B). Two sequences were obtained from Enterococcus sp. Similar to cutC, the chosen HMM similarity cutoff was set at an obvious HMM score drop that, however, correlated less strongly with other parameters investigated in comparison with the cutC analysis shown above. Specifically, 34 lower-scoring sequences from Bacilli are neither in close phylogenetic relation to the top-scoring sequences nor do they exhibit syntenic cntB sequences and were, hence, not considered as true cntA (see also Additional file 1D). All sequences (with one exception) display a conserved “bridging” glutamate instead of aspartate (E205D), which was previously suggested as a signature for that enzyme . Revealed candidates exhibit cntA as a single copy gene, except for 31 genomes, mainly A. baumannii and E. coli strains, where more than one target was detected. However, the majority of those sequences were identical to other cntA genes in the respective genomes, and only two candidates (Klebsiella pneumoniae BIDMC 18C and Cupriavidus taiwanensis STM 6070) exhibited two non-identical cntA sequences.
Gene-targeted assays to investigate the TMA-producing community
Gene-targeted assays for cutC and cntA were developed in order to determine the potential of the gut microbiota to produce TMA and to characterize the composition of this functional community in detail. In silico analysis on coverage of developed primers suggested that a broad range of cutC and cntA gene sequences are targeted, namely 93 and 96% of all unique cutC and cntA genes, respectively (allowing for one mismatch in each primer). To test actual primer performance on various targets in vitro, short synthetic sequences that contained the target sequences from distinct taxa covering a broad thermodynamic range were used as a template in qPCR reactions. For both genes, all tested synthetic sequences were amplified and, with a few exceptions, quantified at similar abundance levels (Additional file 3C, D ). Based on genomic sequences that were used as standards in the developed assays (see the “Methods” section), a quantification limit of 5 × 103 genome copies per reaction for both genes was determined. Below those concentrations, the formation of primer dimers did not allow for accurate target enumeration (Additional file 3A, B).
In order to characterize the TMA-producing community in detail, amplified products were sequenced on the Illumina MiSeq platform. CutC genes similar to those previously observed in all three phyla presented in the database, namely Actinobacteria, Firmicutes, and Proteobacteria, were detected. On average, 76 ± 18 clusters per sample (90% nucleotide identity) were observed, where the majority was similar to genes previously observed in Firmicutes, in particular to those of Clostridium XIVa strains and Eubacterium sp. strain AB3007 (Fig. 2b). However, a major part of sequences, especially those of clusters a–k (Fig. 2b), were only distantly related to reference sequences and formed separate clades on the tree. Consequently, overall nucleotide similarity of cutC amplicons to reference sequences was low (86% ± 7%). Detailed analysis applying high clustering identity cutoffs (99 and 98% nucleotide similarity) revealed high diversities of the cutC gene with several thousand distinct sequence types that are shared between a few samples (<10%; Additional file 6A). At a cutoff level of 95% 1310 distinct sequence types could be differentiated. Only a few of them (41) were detected in the majority of samples; however, they comprised the bulk of obtained sequences (≥67%). Even at the low identity cutoffs of 90%, the vast amount of clusters was only detected in a minority of samples with many being sample specific.
In contrast, all cntA sequences obtained were similar to those previously observed in Gammaproteobacteria, in particular from Escherichia/Shigella (Fig. 2c); closest match analysis revealed 98.1% being closely related to sequences from E. coli (data not shown). A few communities additionally contained cntA amplicons similar to those previously detected in Citrobacter and Klebsiella. Overall, the identity of amplicons to reference sequences was high (99.5% ± 0.5%), and with 3 ± 2 clusters (90% nucleotide identity) detected per sample, the gene diversity was much lower compared to that observed for cutC. At higher cutoffs (99 and 98% identity), several thousand clusters were detected and richness drastically declined with decreasing identity cutoffs. For example, only 55 and 14 total clusters were detected at 95 and 90% cutoff values, respectively. Of those, a few clusters (nine and one, respectively) were observed in >90% of all samples comprising the majority of obtained sequences (Additional file 6B).
To assess whether taxonomic markers can predict the TMA-production potential of intestinal communities, results from the functional assays were compared to data derived from 16S ribosomal RNA (rRNA) gene sequence analysis. No significant correlations between gene abundances of cutC with relative abundances of respective taxa previously described to contain those genes were observed (Additional file 7). Specifically, Clostridium XIVa and Desulfovibrio relative abundances based on 16S rRNA gene sequence analysis were dissimilar to cutC gene abundances obtained with our assay. Eubacterium was only detected at very low relative abundances in a few samples. Conversely, in most cntA-positive samples (based on qPCR), Escherichia/Shigella and Enterobacteriaceae were detected by 16S rRNA gene sequence analysis.
Screening of metagenomes for TMA-producing catabolic genes
In order to gain insights into TMA-producing communities of atherosclerotic individuals, we reanalyzed metagenomes provided by Karlsson and colleagues , who specifically investigated the microbial composition of symptomatic atherosclerosis patients (n = 12) and compared them to healthy control samples (n = 13). Our results indicated increased abundances of bacteria exhibiting the potential to produce TMA (by about a factor of 2) in the patient group where the abundances of key genes of both pathways were elevated (median values of 0.39 and 0.11% (patient group) versus 0.22 and 0% (controls) for cutC and cntA, respectively, as a percentage of bacteria calculated to exhibit the genes) (Additional file 8). However, these observed differences were statistically not significant. Sequencing depth was roughly 1 order of magnitude lower compared to HMP datasets hampering accurate analyses.
Genomic analyses of Clostridium XIVa strains
Ecophysiological insights on key TMA producers and specification of their niches in vivo assist to understand factors that control their abundance and might, eventually, help to design specific treatment strategies for containment of those bacteria. Whereas a wealth of information is available for the main cntA/B-exhibiting taxon, E. coli, much less is known for members of the diverse genus Clostridium XIVa whose cutC sequences represent, together with that of Eubacterium sp. strain AB3007, the closest known relatives of the majority of obtained amplicons as well as metagenomic reads associated with the gene. Phylogenetic analyses based on 16S rRNA gene sequence similarities demonstrated CutC-encoding Clostridium XIVa strains as a polyphyletic group spanning the entire genus intermitted by strains lacking that gene (Additional file 9A). Overall, Clostridium XIVa cutC protein sequences form a monophyletic clade (Additional file 1), where phylogenetic relations, with a few exceptions such as Clostridium phytofermentans, Clostridium sp. AT5, and Dorea sp. 5-2, resemble those derived from 16S rRNA gene sequence analysis (Additional file 9B) suggesting a close linkage between host phylogeny and cutC gene evolution. This indicates that gene loss rather than horizontal gene transfer is driving the disparate ability to transform choline into TMA within strains of this genus.
Results of global feature analysis based on FIGfams largely reflected 16S rRNA gene phylogeny where cutC-containing strains span the entire dendrogram and are dominated by three separate groups (Additional file 10A). C. phytofermentans, Clostridium sp. AT5, and Dorea sp. 5-2 are clustering apart displaying ~55% functional similarity with members of the three main groups, while Eubacterium sp. strain AB3007, together with three additional strains, forms the outgroup sharing less than half of FIGfams with other strains. More specific functional predictions were performed based on IMG’s phenotype analysis as well as by screening genomes for carbohydrate-active enzymes (CAZymes). The former analysis suggested that strains are auxotrophic for several amino acids (on average, 11 ± 2; Additional file 10B). Since complex polysaccharides that resist adsorption in the upper gastrointestinal tract form the major energy source for colon-living bacteria and several Clostridium XIVa strains are reported to grow on such compounds, their functional repertoire of CAZymes was of specific interest. All strains are predicted to exhibit CAZymes linked to the break-down of various diet and host-derived substrates (panel C in Additional file 10). Overall, strains are predicted to contain 101 ± 79 glycoside hydrolases (GHs), 41 ± 13 glycosyltransferases (GTs), 25 ± 17 carbohydrate-binding modules (CBMs), 26 ± 13 carbohydrate esterases (CEs), and 4 ± 4 polysaccharide lyases (PLs).
To analyze the human intestinal TMA-producing bacteria, we established a framework consisting of databases for the key genes of the major TMA-forming pathways and gene-targeted assays for their enumeration. Given that 16S rRNA gene sequence analysis cannot adequately predict the TMA-producing potential (Additional file 7) and metagenomic data lack depth to satisfactorily resolve this low abundant functional community, the assays developed here represent the most proper technique to investigate intestinal TMA-producing communities. With this in hand, we were able to quantify potential colonic TMA producers and gain detailed insights into their diversity.
Any comprehensive functional analysis of entire communities requires accurate definition of biochemical pathways and associated genes. Two distinct enzyme clusters involved in TMA production with the key genes cutC  and cntA  were previously described. A third enzyme, namely YeaW that is widespread in E. coli, was recently proposed as the central enzyme of a third major pathway for the conversion of carnitine to TMA . However, the yeaW gene displays close sequence identity (74%) to the cntA model sequence from A. baumannii and is arranged in similar operons that additionally encode a reductase (yeaX and cntB, respectively) as well as a betaine/carnitine/choline transporter, and for both enzymes, promiscuity to transform carnitine and butyrobetaine at similar rates was shown (yeaW/X was reported to act on choline and to a lesser extent on betaine as well). In this study, we, hence, considered YeaW as belonging to the CntA cluster of enzymes as originally proposed by Zhu and colleagues . Importantly, a recent report based on a genome-screening approach suggesting that numerous taxa contain both cntA/B and yeaW/X is misleading  as the database developed in this study unequivocally shows that the vast amount of genomes exhibits a single copy of this gene group.
The established databases present a comprehensive update of previous cutC  and cntA  gene sequence collections adding a multitude of new gene sequences. They will be regularly updated to serve as a resource for other researchers as well. Our gene-targeted assays encompass key genes of the two major TMA-synthesis pathways and target a broad range of distinct taxa. Primer coverage is thorough, and amplification as well as quantification of numerous distinct target sequences at similar levels was confirmed. Nevertheless, some sequences comprised in our databases are not expected to be amplified, as the cntA forward primer does not match a few Betaproteobacteria, and in the case of cutC, a few sequences observed in Streptococcus show ≥2 mismatches with the reverse primer. However, for both genes, metagenomic analysis suggested that sequences not targeted by our assays do not play an important role in the colonic TMA-producing communities as not a single read was related to any of those reference sequences. It should be noted that for amplification of functional genes, high degeneracy of primers is needed in order to ensure detection of the entire diversity. Since this can result in unspecific binding (in particular seen here for cntA), we suggest to quality control for every sample (see Additional file 4) in order to draw accurate conclusions on target abundance.
Target genes were detected in all samples suggesting that TMA producers constitute members of the core gut community, though at very low abundances. In the HMP dataset, 25% of samples were negative for cutC/D and cntA/B genes, which is most probably due to a lack of sequencing depth. The high diversities of possible TMA producers observed in fecal samples, in particular of cutC gene sequences, demonstrate that individual communities are characterized by vast functional redundancies where several taxa potentially contribute to the TMA pool. Sequences associated with three distinct phyla were detected, but the majority were associated with those observed in members of the Clostridium XIVa (and a specific Eubacterium). Although we detected competition patterns between related taxa, in particular between clusters a and b where either taxon dominated in specific samples (Fig. 2), co-occurrence of various phylogenetically close TMA-producing genes was observed in most samples. Functional predictions on respective reference genomes might explain those observations as they indicate broad, only partly overlapping ecological niches of individual strains. More specifically, our analyses indicated that Clostridium XIVa strains are characterized by overproportional rich carbohydrate-active enzyme (CAZyme) repertoires within the Lachnospiraceae  where, next to dietary matter, host-derived mucin might serve as a major nutrient source. Global feature analysis based on FIGfams suggested large functional differences between members of Clostridium XIVa. However, the bulk of obtained cutC sequences was distantly related to known references of this genus, and predictions presented here can, hence, only partially define functional characteristics of the TMA-producing community.
The observation of highly diverse genes potentially encoding TMA-forming enzymes has direct medical implications as it demonstrates the complexity of the TMA-synthesizing source and demands precision treatment where whole consortia rather than single bacterial taxa need to be targeted in order to restrict production of this health hazardous compound. Our analyses identified distinct groups of TMA-producing gene pools that are each dominated by a few sequence types, similar to enterotypes of global intestinal communities , which might guide the development of type-specific intervention strategies. It should be mentioned that major health-promoting butyrate producers previously classified as belonging to Clostridium XIVa such as Eubacterium rectale and Roseburia represent members of other genera that does not comprise any known CutC-encoding strains.
TMA formation via cntA represents a distinctive branch of TMA synthesis where, next to the enzyme’s distinct substrate spectrum compared with the choline TMA-lyase, ecophysiologic features of its key player, E. coli, are substantially different to those of the major cutC-exhibiting taxa. In particular, its facultative anaerobic nature, the lack of amino acid auxotrophies, and a restricted CAZyme repertoire (data not shown) demonstrate that this taxon thrives in distinct niches/environments compared with bacteria containing cutC. Thus, specific conditions that select against the latter bacteria might be compensated by an increase of taxa encoding CntA . In fact, results of the two pathways did not correlate, which is highlighted by two samples derived from the HMP dataset that display exceptionally high amounts of cntA, but only minute cutC abundances (Fig. 4). This emphasizes the need to target the entire TMA-production potential of gut communities including all major pathways and taxa involved in order to obtain adequate functional assessments.
Samples used in this study derived from volunteers that differed substantially in various characteristics such as age, BMI, and health status (Additional file 5), which allowed insights into the TMA-producing community from a diverse population, but hindered revealing specific host factors that govern its proliferation. Furthermore, we only targeted the TMA-producing potential by quantifying respective gene abundances, and no investigations on gene expression/TMA production were performed limiting insights into actual functional activity. Reanalysis of the dataset provided by Karlsson and colleagues  indicated elevated abundances of potential TMA producers in atherosclerosis patients, though the methodology used, i.e., metagenomics, provided only limited insights and together with a low sample size it did not allow for extraction of statistically significant results (Additional file 8). Our developed assays are applicable in high throughput providing the basis for comprehensive studies investigating the link between gene abundance/expression, substrate availability, and TMA/TMAO serum concentrations in defined population groups, such as patients suffering from atherosclerosis. Next to characterizing the TMA-producing communities of risk groups, studies investigating specific factors governing the TMA-producing community are needed. In particular, diet is of interest as a correlation between dietary habits and production of TMAO was previously established. For instance, individuals on an omnivorous diet produced elevated TMAO levels in comparison to vegans or vegetarians following ingestion of l-carnitine . Furthermore, patients suffering from trimethylaminuria, where TMA accumulates in various body fluids, are often treated by adapting to a vegan diet in order to limit the intake of dietary animal products that promote TMA production such as egg yolk and various meats . The predicted profound amino acid auxotrophies of major players of the cutC-containing community (Additional file 10) suggest a link between the host diet, proliferation of TMA-producing strains, and concurrent TMA synthesis. Specifically, a nutrition that is rich in animal-derived products delivers both essential amino acids required for growth and high concentrations of the main TMA precursors choline and carnitine. In this context, it should be noted that it is unclear whether carnitine or choline itself are relevant selection factors for TMA-producing bacteria. Most bacteria, also those belonging to Clostridium XIVa as well as main cntA-containing taxa are able to utilize a multitude of energy sources in vivo where TMA precursors contribute, most probably, only minutely to the entire substrate pool, which is supported by the large CAZyme repertoires predicted for major TMA-producing taxa (see above).
This study provides a diagnostic framework comprising comprehensive databases and gene-targeted assays that allowed, for the first time, the quantification and detailed characterization of the TMA-producing potential in human fecal samples. All subjects contained target genes where a multitude of potential TMA-producing taxa were revealed in most samples that, overall, clustered into distinct types characterized by highly abundant key players. Results of the developed assays together with presented functional predictions of major taxa involved provide crucial information for uncovering factors limiting growth of TMA producers in the gut and may assist the design of subject-specific therapies that prevent TMA synthesis and the development of disease.
Constructing the databases
Databases for the key genes cutC and cntA were built using a multiparametric approach similar to that previously described for butyrate producers . A selection of protein reference sequences from literature [6, 7] (GenBank accession no.: cutC, ACL49259, ABB40076, EDU36695, EEU12078, EFJ62362, EEW38822, EEI47333; cntA, EFF74816, EGJ58898, EEW98385, EHL80614, EFK52065, EEW40126, EHL93080, EGQ26304) were aligned in Clustal Omega  and used to construct hidden Markov models (HMM) on full-length proteins via hmmbuild (default mode, HMMER 3.1b1, hmmer.org). Model seed sequences were realigned to the model using hmmalign (default mode) and manually checked in Jalview (jalview.org, v. 2.8.1) before rebuilding models based on the obtained alignments; this cycle was repeated three times to ensure construction of robust models. Subsequently, all 67,134 genomes provided by PATRIC (from https://www.patricbrc.org; April 2016) were screened (hmmsearch (--tblout)) for respective protein sequences. The top 3000 and 10,000 protein sequences (based on HMM score) from cutC and cntA searches, respectively, were collapsed (function derep ) and aligned to the HMM models (hmmalign in default mode). Partial gene sequences (<80% coverage to respective HMM models) were omitted, and conserved amino acid residues that were previously proposed as signature sites for the individual genes [6, 7] were determined manually for all sequences from the alignment files in Jalview. To infer phylogenetic distances to each top sequence of the two genes (based on HMM score from hmmsearch results), phylogenetic trees were constructed from the alignments (after conversion to fasta format using function to-fasta ) with the program FastTree (v. 2.1.8)  using the JTT+CAT model; pairwise distances between branch tips were determined using cophenetic.phylo function in R (v. 3.1.2) (package: ape, v. 3.4). Finally, genomes were screened for cutD and cntB (hmmsearch) using HMMs that were built (hmmbuild, hmmalign cycles as described above) from protein sequences that derived from the same genomes that contain the cutC/cntA seed sequences used above (to obtain seed sequences, reference sequences of cutD (ACL49260) and cntB (EEX03957) were BLASTED (blastp, v. 2.2.28+) against respective genomes; all seed sequences were in direct synteny (based on locus tag) with respective partner genes cutC and cntA; no cntB for Sporosarcina newyorkensis was found). Sequences were sorted based on HMM similarity scores, and low cutoffs at the end of obvious HMM score drops (HMM score of 51 and 26% of the top-scoring cutD and cntB sequences, respectively) were set to define putative cutD/cntB genes. Subsequently, their synteny with respective partner genes cutC and cntA was determined where genes separated by ≤10 genes (based on locus tag) were considered as syntenous. Due to the high consistency of all parameters investigated for cutC, HMM similarity cutoffs were chosen as the sole selection criterion resulting in 1114 candidate genes (454 unique proteins). For cntA, phylogeny was applied as an additional filter to the HMM score and 43 sequences (34 unique proteins; including S. newyorkensis) that displayed HMM similarities above the set cutoff were omitted yielding 6738 genomes that exhibit 6772 candidate genes (491 unique proteins). For taxonomic assignments, 16S rRNA gene sequences were collected from all genomes using a HMM model  and the longest sequence from each genome was subjected to the RDP classifier (--format = fixrank) applying a confidence interval of 80% . For a few genomes that displayed only short sequences (<900 bp), the NCBI taxonomy was used as provided by PATRIC; for a few Clostridium XIVa-type strains, only short 16S gene sequences were obtained and RDP taxonomy was manually added. All genomes of candidates with respective taxonomy are listed in Additional file 2.
Sample collection and DNA extraction
Human fecal samples from 50 volunteers were analyzed. No specific exclusion criteria based on health status of the volunteers were set. Their metadata is presented in Additional file 5. Many subjects were diagnosed with hypertension (28%), but none were known to suffer from atherosclerosis or severe cardiovascular disease. Samples were collected in a disposable kidney dish, and aliquots were transferred to 15-mL collection tubes (RNAlater solution added within a few hours after sampling (Sigma-Aldrich, Munich, Germany)) and stored at −80 °C. DNA was extracted using the FastDNA™ SPIN Kit for Soil (MP Biomedicals, Heidelberg, Germany) according to the manufacturer’s instruction and purified using the QIAquick PCR Purification Kit (Quiagen, Hilden, Germany). Nanodrop analysis (260/280 and 260/230 ratios) was performed as a crude quality control before analysis, and DNA concentrations were quantified with the 2030 Multilabel Plate Reader VICTOR X3 (Perkin Elmer, Waltham, MA, USA) using the Quant-iT Picogreen dsDNA assay kit (Invitrogen, Carlsbad, CA, USA).
Development of gene-targeted assays
Primers targeting cutC and cntA as well as the V1-2 region of the 16S rRNA gene (with modified forward primer) are shown
Forward primer (5′➔3′)
Reverse primer (5′➔3′)
In order to characterize the TMA-producing potential of microbial communities via sequencing, a three-step approach for library preparation was developed (Additional file 11) based on the dual index Illumina (San Diego, CA, USA) MiSeq sequencing procedure using the TrueSeq protocol. The genes cutC and cntA were amplified using the primers and amplification conditions as described above for qPCR (applying only 20 cycles). Products were purified (PureLink PCR Purification Kit; Invitrogen, Darmstadt, Germany) to remove primer dimers and 1 μL of the purified product served as template in a second PCR (seven cycles) using identical amplification conditions as in step one but applying target primers with a short overhang (Additional file 11). The PCR products were purified again (PureLink PCR Purification Kit; Invitrogen, Darmstadt, Germany) and subjected to a third amplification step that added the two indices and Illumina adapters to amplicons using PrimeSTAR HS DNA Polymerase (Takara, Otsu, Shigu, Japan) according to the manufacturer’s instructions. Thermocycling was performed as follows: 3 min at 95 °C, 10 s at 98 °C, 10 s at 55 °C, 45 s at 72 °C (×30), and 2 min at 72 °C. This step was specifically performed at high cycle numbers amplifying targets to adequate concentrations for sequencing as the deployed primers were non-degenerate minimizing possible amplification-derived bias. Amplicons were separated by gel electrophoresis, and the appropriate size was excised (cutC ~480, cntA ~460) and extracted with the QIAquick Gel Extraction Kit (Qiagen, Hilden, Germany). Obtained products were quantified by the Quant-iT Picogreen dsDNA assay kit as described above and pooled in equimolar ratios before sequencing on Illumina MiSeq (2 × 250 paired ends).
Bioinformatic procedures for amplicon analysis
After quality filtering (Read Q25) and merging of raw paired-end reads , primers were trimmed off and sequences were subjected to FrameBot (v. 1.2, in default mode with option stat -t hist) analysis where all amplicons were aligned to reference sequences and frame-shift corrected as required . The reference sets for each gene contained all unique sequences considered as cutC and cntA genes, respectively, as well as the 300 top-scoring unique protein sequences below the set HMM thresholds representing distinct sequences (see the “Constructing the databases” and “Results” sections; the 34 sequences derived from Bacilli located above the threshold though not considered as true cntA were included as well) as previously performed for butyrate producers . This allowed a clear differentiation of amplicons that showed low similarity to reference sequences into those probably encoding enzymes for TMA production and others that are considered not to encode this function, which were not included in follow-up analyses. Sequences that displayed a stop codon were omitted. On average, 98.2% (cutC) and 92.5% (cntA) of all merged sequences passed these initial steps resulting in a mean of 40,915 ± 12,376 cutC sequences per sample (from 23,696 to 71,952) and 42,079 ± 7746 cntA sequences (from 28,471 to 52,687) derived from 50 and 12 samples, respectively. For cntA, sample 3 was omitted as >99% of obtained sequences (from 13,095) failed FrameBot analysis (this sample was also on the borderline of detection in qPCR and produced only a faint band during PCR amplification). For subsequent complete-linkage clustering (default mode ), the translated protein sequences were aligned to the HMM models and obtained alignments served as references to align the corresponding nucleotide sequences (function align-nucl-to-prot ), which were then subjected to clustering (at 90% nucleotide identity). All reference sequences from databases were trimmed to the amplicon region and included in this procedure. Only clusters that exhibit ≥5 sequence counts were considered, and the data was rarified to equal depth using the function rarefy_even_depth (rngseed = TRUE) from the R phyloseq package (v. 1.10.0)  before follow-up analyses. To retrieve the nucleotide identity of amplicons to references, sequences were BLASTED (blastn, v. 2.2.28+) against our databases and their identity was recorded. Heatmaps were created in R (v. 3.1.2) using the package gplots (v. 2.17.0) on logarithmic, relative abundance data (log(x + 1)). Neighbor-joining trees were constructed in MEGA (Molecular Evolutionar Genetics Analysis; version 6.0) applying 500 bootstraps using p-distance corrections and pairwise deletion of gaps and missing data. Co-occurrence analysis was performed as previously described  with data derived from the complete-linkage clustering result. Only significant correlations (p < 0.01) displaying a ρ ≥ 0.5 and a false-discovery correction q-value <0.01 between clusters that were detected in at least 50% of samples were considered for analysis. Non-metric multidimensional scaling analysis was performed in R (package: vegan, v. 2.3-4); cluster abundances were manually superimposed.
Quality filtered sequence reads that were derived from 154 stool samples provided by the Human Microbiome Project (HMP ; data available at http://hmpdacc.org) were subjected to BLAST (blastall, -p blastn, v. 2.2.26) searches against the developed databases containing cutC/D and cntA/B genes. As for the amplicon analysis above, the reference set of each gene additionally included sequences that were below the set HMM threshold (500 top-scoring unique nucleotide sequences; for cutD and cntB, the threshold was defined by the lowest scoring gene that was syntenous with its partner gene; sequences derived from Bacilli located above the threshold though not considered as true cntA were included as well). Top hits (based on e-value) that covered ≥70 bp and displayed ≥70% identity to a reference were considered for analysis where both genes of each pathway had to be detected for a positive result. Median abundances of both genes were used to calculate pathway abundances expressed relative to the abundance of the housekeeping gene rplB (gene-length corrected), which had been extracted from all genomes based on a HMM model provided by RDP . Only samples displaying both genes of a pathway were considered for analysis. Prior to taxonomic analysis of reads, the cutC/D reference genes were subjected to complete-linkage cluster analysis (90% identity on the nucleotide level  in default mode) as previously described , whereas sequences of cntA/B were taxonomically binned on the genus level. Median abundances of both genes of a pathway were used to calculate relative gene abundances associated with individual taxa. Furthermore, a recently developed gene-targeted metagenomic assembly tool that was specifically optimized for low target concentrations was used to screen samples (in default mode with a k-mer size of 45, a minimum contig size of 150 aa, and normalization of results to rplB abundance) . As for the BLAST analysis, only samples containing both genes of a pathway were considered for analysis.
16S rRNA gene sequence profiling of samples
In order to compare results obtained with the gene-targeted approach with taxonomic data, the V1-2 region of the 16S rRNA gene was amplified from all samples. To ensure adequate amplification, including taxa that were previously underrepresented using conventional primers that target the V1-2 region such as Bifidobacteria , a modified forward primer was used (Table 1). One microliter of template DNA from extract was directly amplified using primers containing the overhangs (Additional file 11). Amplification was performed with the PrimeSTAR HS DNA Polymerase (Takara, Otsu, Shigu, Japan) according to the manufacturer’s instructions. Samples were denaturated for 3 min at 95 °C following 15 cycles of denaturation at 98 °C for 10 s, annealing at 58 °C for 10 s, and extension at 72 °C for 45 s. One microliter served as the template in a second 10-cycle PCR step adding the two indices and Illumina adapters to amplicons using amplification conditions as described above. Amplified products were purified, normalized, and pooled using the SequalPrep Normalization Plate and subjected to 250-bp paired-end Illumina MiSeq sequencing.
Raw reads were merged, filtered for a length of ≥250 bp, and subjected to the RDP classifier (--format = fixrank) . Data was analyzed at the genus level. In order to obtain more detailed insights into the diversity of Clostridium XIVa-associated taxa, all sequences matching this taxon at a confidence interval ≥80% were additionally aligned to a reference set containing 16S rRNA gene sequences from all genomes classified as Clostridium XIVa (see above) using the RDP aligner (pairwise-knn)  and the top-scoring reference of each sequence was recorded.
Genomics of Clostridium XIVa genomes
For global functional comparisons between strains, FIGfams (provided by PATRIC) were extracted from respective genomes; the cluster analysis based on binary Bray-Curtis dissimilarity calculations and construction of the associated dendrogram was performed in R (package: vegan, v. 2.3-4). Prediction of carbohydrate-active enzymes (CAZymes) was performed using family-specific HMMs provided by dbCAN  according to the developer’s instructions with default parameters. Amino acid auxotrophies were extracted from the phenotype analysis of corresponding entries on the Integrated Microbial Genomes database . For all analyses, the genome of Eubacterium sp. AB3007 (1392487.3) was included.
We thank Qiong Wang for her support on the use of Xander, Jiarong Guo for providing the HMM for the 16S rRNA gene, Diego Chavez Moreno for input on genomic analyses, and Iris Plumeier and Silke Kahl for technical assistance. Thanks to Robert Geffers for helping to establish the procedures for library preparation and Illumina sequencing.
This study was supported by iMed, the Helmholtz Association’s Initiative on Personalized Medicine.
Availability of data and materials
Databases for cutC and cntA sequences are available at http://18.104.22.168/TMA/ and will be regularly updated. Raw sequences were submitted to the European Nucleotide Archive (ENA) under accession number PRJEB20259.
MV, SR, BH, and DP conceived and designed the experiment. SR and MV performed the experiments. MV and SR handled the data analysis. MV, SR, and DP wrote the paper. SR, BH, DP, and MV handled the article revision. All authors read and approved the final manuscript.
The authors declare that they have no competing interests.
Consent for publication
Ethics approval and consent to participate
Procedures of the study have been approved by the local ethics committee of the Hannover Medical School (vote no. 2087-2013), and all participants signed a written informed consent form.
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