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Table 1 Primers targeting cutC and cntA as well as the V1-2 region of the 16S rRNA gene (with modified forward primer) are shown

From: Uncovering the trimethylamine-producing bacteria of the human gut microbiota

  Forward primer (5′➔3′) Reverse primer (5′➔3′)
cutC TTYGCIGGITAYCARCCNTT TGNGGYTCIACRCAICCCAT
cntA TAYCAYGCITGGRCITTYAARCT RCAGTGRTARCAYTCSAKRTAGTTRTCRAC
16S AGRGTTHGATYMTGGCTCAG TGCTGCCTCCCGTAGGAGT
  1. Bold letters highlight the degenerate bases within sequences (total combinations—cutC_F, 32; cutC_R, 16; cntA_F, 32; cntA_R, 512; 16S_F, 32; 16S_R, 1). Expected sizes of amplified products (excluding primers) are 275 bp (cutC) and 249 bp (cntA) based on references ACL49259 and EFK52065, respectively