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Utilizing a reductionist model to study host-microbe interactions in intestinal inflammation

Microbiome volume 9, Article number: 215 (2021) Cite this article

Abstract

Background

The gut microbiome is altered in patients with inflammatory bowel disease, yet how these alterations contribute to intestinal inflammation is poorly understood. Murine models have demonstrated the importance of the microbiome in colitis since colitis fails to develop in many genetically susceptible animal models when re-derived into germ-free environments. We have previously shown that Wiskott-Aldrich syndrome protein (WASP)-deficient mice (Was−/−) develop spontaneous colitis, similar to human patients with loss-of-function mutations in WAS. Furthermore, we showed that the development of colitis in Was−/− mice is Helicobacter dependent. Here, we utilized a reductionist model coupled with multi-omics approaches to study the role of host-microbe interactions in intestinal inflammation.

Results

Was−/− mice colonized with both altered Schaedler flora (ASF) and Helicobacter developed colitis, while those colonized with either ASF or Helicobacter alone did not. In Was−/− mice, Helicobacter relative abundance was positively correlated with fecal lipocalin-2 (LCN2), a marker of intestinal inflammation. In contrast, WT mice colonized with ASF and Helicobacter were free of inflammation and strikingly, Helicobacter relative abundance was negatively correlated with LCN2. In Was−/− colons, bacteria breach the mucus layer, and the mucosal relative abundance of ASF457 Mucispirillum schaedleri was positively correlated with fecal LCN2. Meta-transcriptomic analyses revealed that ASF457 had higher expression of genes predicted to enhance fitness and immunogenicity in Was−/− compared to WT mice. In contrast, ASF519 Parabacteroides goldsteinii’s relative abundance was negatively correlated with LCN2 in Was−/− mice, and transcriptional analyses showed lower expression of genes predicted to facilitate stress adaptation by ASF519 in Was−/−compared to WT mice.

Conclusions

These studies indicate that the effect of a microbe on the immune system can be context dependent, with the same bacteria eliciting a tolerogenic response under homeostatic conditions but promoting inflammation in immune-dysregulated hosts. Furthermore, in inflamed environments, some bacteria up-regulate genes that enhance their fitness and immunogenicity, while other bacteria are less able to adapt and decrease in abundance. These findings highlight the importance of studying host-microbe interactions in different contexts and considering how the transcriptional profile and fitness of bacteria may change in different hosts when developing microbiota-based therapeutics.

Video abstract

Introduction

Inflammatory bowel diseases (IBD) are a group of heterogeneous chronic inflammatory disorders that affect the gastrointestinal tract. The etiology of IBD is complex and multifactorial, with genetic, immunological, environmental, and microbial factors all contributing. Genome-wide association studies have identified more than 240 loci associated with a risk of IBD, and many of these loci implicate pathways involved in immunological responses to bacteria [1,2,3]. The intestines, particularly the colon, are inhabited by a high density of bacteria, and many lines of evidence suggest that these microbes, known as the intestinal microbiota, play an important role in IBD. For example, the composition of the intestinal microbiota is altered, or dysbiotic, in patients with IBD compared to healthy controls, although the specific differences are highly variable among studies [4]. Furthermore, antibiotics, fecal stream diversion, and fecal microbiota transplants have all been shown to be somewhat efficacious in the treatment of IBD, although our ability to draw definitive conclusions about their efficacy has been hindered by heterogeneous study designs [5,6,7,8,9,10]. In order to improve upon microbial-based therapeutics for IBD, we need to develop a better understanding of how gut microbes initiate and modulate intestinal inflammation and how inflammation shapes the gut microbiota.

Human microbiome studies can be difficult to interpret due to variation in genetics, diet, and other environmental factors that shape microbial composition aside from the examined disease state. It is also challenging to obtain samples from patients prior to the onset of disease or longitudinally thereafter, limiting the conclusions that can be drawn from these studies. In contrast, murine models of disease lack much of this confounding complexity, and their microbiota can be user defined and longitudinally sampled. Murine studies have been instrumental in establishing the importance of the gut microbiota in initiating colitis, since colitis fails to develop in nearly all murine models of IBD when mice are re-derived in germ-free environments [11, 12]. In addition, the colitis phenotype of certain murine models is communicable by transfer of the gut microbiota [13, 14]. Murine models have also facilitated the identification of immunomodulatory molecules produced by gut bacteria such as short-chain fatty acids [15,16,17] and capsular polysaccharide A [18]. However, the design of rigorous, well-controlled microbiome studies in mice is often limited by the large number of bacterial species involved and inter-individual variation in gut microbial communities. To address this, we have developed a reductionist model using a simplified and defined microbial consortium in a genetically susceptible host.

Similar to humans, mice lacking the Wiskott-Aldrich syndrome protein (WASP) (Was−/− mice) exhibit immune cell defects leading to gut inflammation [19]. Furthermore, colitis in Was−/− mice is microbiota-dependent. WASP is a hematopoietic-specific protein, encoded on the X chromosome that regulates Arp2/3-dependent actin polymerization, and mice and humans deficient in WASP exhibit defects in both their adaptive and innate immune systems [20, 21]. WASP-deficient patients develop a primary immunodeficiency characterized by recurrent infections, eczema, and thrombocytopenia, and approximately 10% develop an IBD-like colitis [22]. Was−/− mice on the 129SvEv background housed under specific pathogen-free (SPF) conditions containing Helicobacter species reliably develop a spontaneous lymphocyte-dependent colitis [23, 24]. SPF mice contain a complex microbial community that is free of particular disease-causing microbes. When Was−/− mice are re-derived in SPF conditions free of Helicobacter, they no longer develop spontaneous intestinal inflammation. Following colonization of re-derived SPF Was−/− mice with Helicobacter bilis, they develop typhlitis and colitis, with a subset exhibiting dysplasia and even colon carcinoma [24]. In this study, we show that mono-colonization of Was−/− mice with H. bilis is not sufficient to induce intestinal inflammation, indicating that H. bilis requires the presence of other bacteria to elicit colitis in Was−/− mice. Furthermore, we replace the complex and variable SPF flora with a defined commensal community, the altered Schaedler flora (ASF) [25,26,27], to study how interactions between a pathobiont, the commensal flora, and an immune-dysregulated host result in the initiation and perpetuation of intestinal inflammation.

Results

WASP deficiency is associated with altered fecal microbial composition and outgrowth of pathobionts

We first sought to determine whether gut microbial composition in Was−/− mice differs from wild-type (WT) mice and if so, at what age their microbiota begin to diverge. Stool samples were collected longitudinally from Was−/− and WT mice raised under SPF conditions at 4, 8, 12, 16, and 20 weeks of age. Microbial profiling by 16S rRNA sequencing revealed that the microbiota of WT and Was−/− mice were similar at 4 weeks of age and began to differ by 8 weeks of age, as measured by Bray-Curtis distance (Fig. 1A). Analysis at the phylum level revealed that only the relative abundance of Deferribacteres was consistently and significantly different between genotypes after correcting for multiple taxa comparisons (Fig. S1A,B). Further analysis revealed that the genus Mucispirillum accounted for essentially 100% of the Deferribacteres reads, and the Mucispirillum relative abundance was higher in Was−/− compared to WT mice after week 8 (Fig. 1B). This finding is consistent with prior studies demonstrating outgrowth of Mucispirillum under inflammatory conditions and a role for Mucispirillum schaedleri as a pathobiont that triggers inflammation in an immune-deficient host [28, 29].

Fig. 1.
figure 1

WASP deficiency results in altered composition of the fecal microbiota. A–C Fecal microbial composition of Was−/− (n = 5) and WT (n = 3) mice raised under SPF conditions with weekly bedding exchanges was analyzed monthly between 4 and 20 weeks of age by 16S rRNA gene sequencing. A Compositional dissimilarity between mice of the same genotype compared to mice of different genotypes was assessed by Bray-Curtis distance. Relative abundances of Mucispirillum (B) and H. bilis (C) were determined for each genotype at each timepoint. D SPF Was−/− mice were re-derived in a Helicobacter species free environment (n = 14), and a subset was infected with H. bilis (n = 9). Fecal microbial composition was assessed at 7–9 months post infection. Principal coordinate analysis of Bray-Curtis distances was performed after H. bilis sequences were removed from the dataset. Statistics performed using PERMANOVA (A and D) and Student’s t-test (B and C). *p < 0.05, **p < 0.01

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Given our prior study revealing a critical role for H. bilis in colitis associated with WASP deficiency [24], we examined the relative abundance of this species and found that it was consistently higher in Was−/− compared to WT mice at 8 weeks of age and thereafter (Fig. 1C). Finally, we asked whether the presence of H. bilis leads to changes in other members of the intestinal microbial community. 16S rRNA sequencing of feces collected from Was−/− mice re-derived in a Helicobacter-free environment and a subset of those that were re-colonized with H. bilis showed significant differences in microbial composition, even after removal of sequences attributable to H. bilis (Fig. 1D). Taken together, these findings indicate that WASP deficiency is associated with changes in fecal microbial composition, including expansion of pathobionts such as H. bilis and Mucispirillum, and the presence of H. bilis drives alterations of other members of the microbial community.

Development of a reductionist model to study the role of the gut microbiota in intestinal inflammation

Next, we sought to determine whether other gut bacteria might contribute to the intestinal inflammation observed in Was−/− mice colonized with H. bilis. The complexity and variability of SPF microbiota make rigorous mechanistic studies challenging. Therefore, we sought to establish a reductionist gnotobiotic model using ASF, which is a defined microbial community comprised of 8 members [25,26,27]. Historically, ASF was developed to colonize germ-free mice with a standardized microbiota, and it has been shown to be more functionally representative of wild microbiomes compared to random consortia of similar or larger size [25, 26, 30]. Notably, ASF does contain M. schaedleri, also known as ASF457, which as described above, has been shown to be a pathobiont in certain contexts [29]. Colonization of Was−/− mice with ASF alone did not induce spontaneous colitis (Fig. 2A, B). Mono-colonization with H. bilis also did not induce inflammation in Was−/− mice, but Was−/− mice colonized with both ASF and H. bilis developed inflammation (Fig. 2A, B), similar to our prior work demonstrating colitis development in SPF Was−/− mice colonized with H. bilis [24]. Colonization of WT mice with both ASF and H. bilis was not sufficient to induce colitis (Fig. 2A, B). These findings indicate that spontaneous colitis in Was−/− mice requires the presence of both a pathobiont and other bacteria in the context of an immune-dysregulated host.

Fig. 2
figure 2

Development of intestinal inflammation requires both the pathobiont, H. bilis, as well as commensal bacteria in the context of host immune dysregulation. A, B WT and Was−/− mice were colonized with the indicated gut microbial communities. A Representative H&E-stained formalin-fixed paraffin-embedded proximal colon sections at 20 weeks after colonization. 20× magnification, scale bars = 100μm. B Quantitative histological colitis scores at 20 weeks after colonization (n = 5,4,5,5,6,3,8,8). C–E Germ-free WT/HET (n=28) and Was−/− (n = 21) mice were colonized with the ASF community and a subset (WT/HET (n = 17) and Was−/− (n = 13)) were gavaged with H. bilis. C Spleen weights were measured 20 weeks after gavage with H. bilis. Colonic lamina propria lymphocytes were isolated 20 weeks after infection with H. bilis, and percentages of IL17-A+ CD4 T cells (D) and IL-22+ ILC3s (E) were determined by flow cytometry. Statistics performed using Student’s t-test. *p < 0.05, ***p < 0.001

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The effect of H. bilis on colonic lamina propria immune cells is context dependent

To study the temporal relationship between the onset of inflammation and changes in the intestinal microbiota, we designed an experiment in which germ-free WT, Was+/− (HET), and Was−/− mice were colonized with ASF, with a cohort subsequently infected with H. bilis (Fig. S2A). To transfer the ASF community to germ-free mice, donor ASF-colonized mice obtained from Taconic Biosciences were co-housed with germ-free mice for 2 months (females), or stool-soiled bedding was transferred from ASF donor cages to recipient cages weekly for 2 months (males). 16S rRNA sequencing confirmed successful colonization of the recipient mice with ASF (Fig. S2B). ASF360 Lactobacillus intestinalis was not detected in donor or recipient fecal samples, which is consistent with prior reports [31].

As expected, Was−/− but not WT mice colonized with ASF and H. bilis developed histologic evidence of intestinal inflammation. Inflammation was observed throughout the cecum and colon (Fig. S2C). Spleen weights were also significantly increased in the Was−/− mice colonized with ASF and H. bilis (Fig. 2C). Analysis of colonic lamina propria lymphocytes revealed an increased percentage of IL-17A+ CD4 T cells among total CD4 T cells in colitic Was−/− mice that were colonized with ASF and H. bilis as compared to WT mice colonized with the same microbiota (Figs. 2D and S2D). Notably, addition of H. bilis to WT mice colonized with ASF led to a significant decrease in the percentage of IL-17A+ CD4 T cells compared to WT mice colonized with ASF alone (Fig. 2D), These data are consistent with recent studies showing that Helicobacter species induce regulatory T cells (Treg) that restrain pro-inflammatory T helper 17 (TH17) cells during homeostasis while the same bacteria promote expansion of colitogenic TH17 cells during inflammation [32, 33]. Furthermore, Helicobacter hepaticus has been shown to activate an anti-inflammatory program in macrophages [34].

Group 3 innate lymphoid cells (ILC3s) help maintain intestinal homeostasis and ameliorate inflammation via production of IL-22 [35,36,37]. Helicobacter species have been shown to negatively regulate the proliferation of ILC3s in an immune-deficient background, and consistent with this, we observed a lower percentage of ILC3s among total ILCs in the colonic lamina propria of Was−/− mice that were colonized with ASF and H. bilis as compared to WT mice colonized with the same microbiota [38] (Figs. 2E and S2E). However, H. bilis’s effect on ILC3s is context dependent, and in uninflamed WT mice, H. bilis colonization resulted in an increase in the percentage of ILC3s (Fig. 2E). These findings highlight that the effect of a given microorganism on the immune compartment can vary greatly depending on whether they are in a homeostatic or immune dysregulated environment, and specifically in the case of H. bilis, it induces a tolerogenic response during homeostasis but promotes inflammation in immune dysregulated hosts.

Fecal microbial composition is correlated with degree of inflammation

We next performed longitudinal fecal sample collection from these mice to elucidate the temporal relationship between the onset of inflammation and changes in microbial composition. Intestinal inflammation developed in Was−/− mice within a week after H. bilis gavage, as measured by fecal lipocalin-2 (LCN2) (Fig. 3A). Was−/− mice were able to control the acute inflammation by week 4 but then proceeded to develop persistent inflammation by week 6 (Fig. 3A). As expected, WT mice colonized with ASF and H. bilis (Fig. 3A) and WT or Was−/− mice colonized with only ASF (Fig. S3A) did not show evidence of significant inflammation at any time during the experiment. H. bilis was not detected by 16S rRNA sequencing in stool from mice that were only colonized with ASF (Fig. S3B). We measured overall differences in microbial composition between WT and Was−/− mice colonized with ASF and H. bilis and observed significant differences during weeks 4 and 6, which was just prior to and at the beginning of establishment of persistent inflammation in Was−/− mice (Fig. 3A, B). The differences were primarily due to increased H. bilis and decreased ASF519 Parabacteroides goldsteinii relative abundances in Was−/− mice (Fig. 3C, D). These differences were not due to cage effect, as there were multiple cages of each genotype that were separately housed, and the subset of WT/HET and Was−/− mice that were co-housed still showed differences in H. bilis relative abundance (Fig. S3C).

Fig. 3
figure 3

Correlation between the relative abundance of the pathobiont H. bilis and the degree of intestinal inflammation depends upon the host genotype. Germ-free WT/HET (n=17) and Was−/− (n = 13) mice were colonized with the ASF community and then gavaged with H. bilis. A Development of intestinal inflammation was monitored by measuring fecal LCN2 serially. Fecal microbial composition was assessed longitudinally by 16S rRNA sequencing. B Compositional dissimilarity between mice of the same genotype compared to mice of different genotypes as measured by Bray-Curtis distance. Relative abundance of H. bilis (C) and ASF519 P. goldsteinii (D) over time by genotype. Correlations between log-transformed fecal LCN2 and relative abundances of H. bilis (E) and ASF519 P. goldsteinii (F) for all timepoints. Tests for linear dependence of log-transformed LCN2 on the relative abundance of each bacterial species was done using a linear mixed-effects model, taking into account a mouse-specific random effect (E and F). Wilcoxon’s rank-sum test was used for (A), (C), and (D) and PERMANOVA for (B). *p < 0.05, **p < 0.01, ***p < 0.001

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Next, we assessed whether the fecal relative abundances of H. bilis and ASF519 P. goldsteinii correlated with the degree of inflammation using a linear mixed-effects model on data from all timepoints, taking into account a mouse-specific random effect. We found that H. bilis relative abundance was positively correlated with LCN2 in Was−/− mice (Fig. 3E), consistent with its role as a pathobiont. Even though feces from WT/HET mice had low LCN2 levels overall, we were still able to detect a highly significant negative correlation between H. bilis relative abundance and LCN2 in WT/HET mice. These data mirror the context-dependent effects of H. bilis on TH17 cells (Fig. 2D). We also observed a negative correlation between ASF519 P. goldsteinii fecal relative abundance and LCN2, indicating a possible protective role for this organism (Fig. 3F), as suggested previously [39]. Analyses of the remaining ASF members did not reveal any strongly significant correlations between their fecal relative abundances and LCN2 levels (Fig. S3D-I).

Mucosal-associated ASF457 M. schaedleri abundance is correlated with severity of inflammation

While stool sampling allows for convenient serial assessment of luminal bacteria, mucosal-associated bacteria are different in composition and are likely to be of particular relevance to intestinal inflammation given their close proximity to the host [40, 41]. Furthermore, in murine models of intestinal inflammation and human IBD, bacteria are able to penetrate the inner mucus layer, which usually separates bacteria from epithelial cells under homeostatic conditions [42]. Consistent with this, we observed that in colitic Was−/− mice colonized with ASF and H. bilis, bacteria are able to penetrate the mucus layer and contact the host epithelium (Fig. 4A). When we profiled the composition of the mucosal-associated microbiota by 16S rRNA sequencing at 20 weeks after H. bilis infection, we found that H. bilis and ASF457 M. schaedleri are present at higher relative abundance than in the stool (Fig. 4B). Both of these organisms are known to be mucus-dwelling [43, 44]. We then examined whether the mucosal relative abundances of each of the 3 most abundant bacteria in the mucosal samples correlated with fecal LCN2. We found no correlation for H. bilis, but mucosal ASF457 M. schaedleri correlated positively, and ASF519 P. goldsteinii trended towards a negative correlation with LCN2 in colitic Was−/− mice colonized with ASF and H. bilis (Fig. 4C–E). In WT/HET mice, correlations between the mucosal abundances of each of these 3 bacteria and LCN2 trended in the same direction as in Was−/− mice, but none of these correlations reached statistical significance (Fig. S4A-C). For the remaining ASF members, none of their mucosal abundances correlated with LCN2 in either genotype (Fig. S4D-H).

Fig. 4
figure 4

Colitis in WASP deficiency is associated with breach of the mucus barrier by the intestinal microbiota, and severity of colitis is correlated with the mucosal abundance of ASF457 M. schaedleri. Germ free WT/HET (n=17) and Was−/− (n = 13) mice were colonized with the ASF community and then gavaged with H. bilis. The mucosal-associated microbiota was assessed at 20 weeks post infection. A Proximal colon sections from representative Was+/− and Was−/− mice stained with a universal bacterial probe (EUB338, red), for mucus using the lectin UEA-1 (green), and with DAPI (blue). Distances between epithelial cells and bacteria were quantified for 3 randomly chosen fields of view for each of 5 WT/HET and 4 Was−/− mice. Each data point represents the average of 10 measurements taken across a field of view. Data shown as mean +/− S.E.M. Statistics done by Welch’s t-test. *** p < 0.001. B Comparison between fecal and mucosal-associated microbiota composition based on 16S rRNA sequencing. Pearson’s correlations between log-transformed fecal LCN2 and mucosal relative abundances of H. bilis (C), ASF457 M. schaedleri (D), and ASF519 P. goldsteinii (E) in Was−/− mice

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WASP deficiency is associated with changes in bacterial gene expression

Assessment of microbial composition can provide important insights into how the microbiota respond to and contribute to intestinal inflammation, but these analyses do not consider the fact that bacteria have sophisticated sensory and signal transduction machinery that allows them to alter their transcriptional state in response to environmental cues. Therefore, even identical bacteria when placed in two different environments may behave very differently, and in doing so influence their host in diverse ways. To elucidate how WASP deficiency and/or inflammation may affect bacterial gene expression, we obtained stool samples from 3 mice of each of the 4 cohorts (WT/HET or Was−/−, ASF with or without H. bilis) and performed bacterial metatranscriptomic sequencing. Based on principal component analysis of high variance GO terms, we found that the overall microbial transcriptional profile in WT/HET compared to Was−/− mice differed, even when both inflamed samples containing H. bilis and uninflamed samples without H. bilis were included (Fig. 5A). This suggests that WASP deficiency is associated with changes in microbial gene expression independent of inflammation. Among the highest variance GO terms, we found that pathways upregulated by the microbiota in Was−/− mice included those involved in nucleic acid synthesis, such as ribonucleoside monophosphate biosynthetic process, ribose phosphate diphosphokinase activity, nucleotide biosynthetic process, and pyrimidine nucleotide biosynthetic process (Fig. 5B).

Fig. 5
figure 5

Colitis in WASP deficiency results in microbial transcriptional changes associated with stress adaptation and immunogenicity. Germ-free WT/HET and Was−/− mice were colonized with the ASF community and a subset were gavaged with H. bilis. Feces from 3 mice of each genotype/microbiota combination (WT/HET or Was−/− with or without H. bilis) at 15 weeks after H. bilis gavage were subjected to bacterial metatranscriptomic sequencing. A Principal component analysis of high variance Gene Ontology (GO) terms for all samples. Statistics calculated by PERMANOVA comparing WT/HET with Was−/− samples. B Heatmap of high variance GO terms. Color scale indicates row-wise z-scores of log-transformed CPM values. BP, biological process: CC, cellular component, MF, molecular function. C–E RNA-seq volcano plots showing differential gene expression of H. bilis (C), ASF457 M. schaedleri (D), and ASF519 P. goldsteinii (E) in Was−/− (positive log2(fold change (FC)) compared to WT/HET (negative log2(FC)) mice colonized with ASF and H. bilis. Y-axis shows p-values corrected for multiple comparison. Red dots represent genes that are significantly differentially expressed as determined by the DESeq2 Wald test

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Inflammation in Was −/− mice is associated with transcriptional changes in ASF457 M. schaedleri and ASF519 P. goldsteinii that can perpetuate inflammation

We next examined gene expression within each bacterial species individually. To tease apart contributions due to genotype or presence of H. bilis versus inflammation, we sequenced samples from an early timepoint (1.5 weeks after H. bilis gavage, when inflammation was minimal based on LCN2) and a late timepoint (15 weeks after H. bilis gavage, when significant inflammation had been present for weeks). Unexpectedly, despite H. bilis playing a critical role in inflammation in Was−/− mice, no differences were identified in H. bilis gene expression in WT compared to Was−/− mice either at the early or late timepoint (Figs. 5C, S5A). In contrast, ASF457 M. schaedleri showed multiple differentially expressed genes in colitic Was−/− mice harboring ASF and H. bilis as compared to non-colitic WT mice harboring the same microbiota (Fig. 5D). These differences were not seen when comparing ASF457 M. schaedleri gene expression in the same mice prior to the onset of inflammation (except for clpB) (Fig. S5B) or in Was−/− versus WT mice colonized with ASF alone (Fig. S5D). These data suggest that the transcriptional differences were a result of the inflammatory environment rather than the host genotype or the presence of the pathobiont H. bilis. As discussed below, the genes that were upregulated by ASF457 M. schaedleri in inflamed Was−/− mice, such as flagellin, ClpB, DnaK, and molybdopterin-dependent oxidoreductase, are expected to both improve its fitness in stressful environments, such as the inflamed intestine, as well as increase its immunogenicity (Fig. 5D). This supports a bi-directional model in which the inflamed environment induces the expression of genes that allow ASF457 to thrive, and ASF457 upregulates the expression of genes that stimulate the immune system, further perpetuating inflammation.

For ASF519 P. goldsteinii, we also observed differences in gene expression in colitic Was−/− mice harboring ASF and H. bilis as compared to non-colitic WT mice harboring the same microbiota (Fig. 5E). As with ASF457 M. schaedleri, these differences were not detected when comparing the same mice prior to the onset of inflammation or Was−/− versus WT mice colonized with ASF alone, suggesting that these differences were due to the inflammatory environment (Fig. S5C,E). However, in contrast to what we observed in ASF457 M. schaedleri, ASF519 P. goldsteinii in colitic Was−/− mice had lower expression of genes that would be expected to help it adapt to stressful conditions, such as superoxide dismutase and DNA starvation/stationary phase protection protein (Fig. 5E). This suggests that ASF519 P. goldsteinii may have reduced fitness in inflamed colons. Most of the other ASF members showed less-striking differences in gene expression (Fig. S5F-J).

Discussion

Our study addresses a major area of interest within the field of IBD, which is the cause-and-effect relationship between dysbiosis and intestinal inflammation. We employed a simplified and defined microbiota within a genetically susceptible WASP-deficient host. Some of the strengths of this model include the fact that it requires more than one bacterial species, and the combination of bacteria that induces disease in Was−/− mice does not do so in WT mice, reflecting some of the complexity seen in human IBD. By using a defined microbiota in a gnotobiotic facility, we were able to minimize differences between experimental groups at the start of the study and monitor for unintentional introduction of new microbes to all or a subset of mice during the study. The limited number of bacteria also allowed us to methodically assess all members of the microbiota throughout the study. Furthermore, this murine model is relevant to human disease, as WASP deficiency in humans results in intestinal inflammation in a subset of patients [22].

H. bilis is critical for the development of intestinal inflammation in Was−/− mice, but it is not pathogenic in and of itself, since mono-colonization of Was−/− mice does not result in disease. H. bilis induced mild inflammation as measured by LCN2, a biomarker of inflammation, within a week after introduction into Was−/− mice colonized with ASF. The inflammation was temporarily controlled by week 4 but then re-developed by week 6 and persisted thereafter. In contrast, even at early timepoints, WT mice exhibited no significant inflammation after colonization with H. bilis. H. bilis colonized at similar abundances in WT and Was−/− mice until week 5, when it reached a significantly higher relative abundance in Was−/− compared to WT mice. Given that the Was−/− mice were not inflamed at this timepoint and developed chronic inflammation afterwards, these observations suggest that impaired control of H. bilis infection may contribute to the initiation of chronic intestinal inflammation in Was−/− mice. Our metatranscriptomic analysis indicates that this impaired control is not due to increased production of virulence factors or immunogenic proteins by H. bilis in Was−/− mice.

Given that mucosal-associated bacteria come in closer proximity to the host than luminal bacteria, especially during colitis, it is likely that they are more strongly influenced by the host immune system and contribute more to intestinal inflammation. Consistent with this, a study of treatment-naïve pediatric Crohn’s patients found that microbial dysbiosis was more strongly reflected in the mucosal-associated microbial community as compared to in the stool microbiota [45]. Examination of the mucosal-associated microbiota in our study was limited to the time of sacrifice due to the technical difficulty of obtaining mucosal samples from the proximal colon. In Was−/− mice colonized with ASF and H. bilis, we found that the major members of the mucosal-associated microbiota are H. bilis, ASF457 M. schaedleri, and ASF519 P. goldsteinii. Of these, H. bilis relative abundance was not correlated with LCN2 while ASF457 M. schaedleri relative abundance was positively correlated with LCN2 and ASF519 P. goldsteinii relative abundance trended towards a negative correlation with LCN2. There are several possible explanations for the lack of correlation between H. bilis mucosal relative abundance and LCN2. It is possible that H. bilis is required to trigger inflammation but is less important for maintaining inflammation. There is precedent for a transient requirement for a pathobiont, as transient colonization of TLR5-deficient mice by adherent-invasive Escherichia coli (AIEC) causes chronic inflammation that persists well beyond clearance of the AIEC [46]. In future studies, it would be interesting to develop a method to selectively deplete H. bilis after the onset of inflammation in order to determine whether it is still required in later stages of disease. Another possibility is that H. bilis only needs to be present above a certain threshold abundance in the mucus to support inflammation and more H. bilis does not result in more inflammation. Yet another possibility is that the absolute abundance of H. bilis does in fact correlate with inflammation but because we are measuring relative abundance, this increase is masked by changes in the abundance of other bacteria such as ASF457 M. schaedleri.

Our study indicates that ASF457 M. schaedleri may act as a pathobiont in Was−/− mice once inflammation has been initiated by another trigger, such as H. bilis, and it has been shown to act as a pathobiont in another model of intestinal inflammation [29]. The positive correlation between mucosal ASF457 M. schaedleri relative abundance and LCN2 may reflect a bi-directional relationship in which inflammation leads to expansion of this population due to upregulation of genes that confer improved fitness, and expansion of this population further perpetuates inflammation due to upregulation of genes that stimulate the immune system. The identities of the genes that were upregulated in ASF457 M. schaedleri in colitic Was−/− mice after but not prior to the onset of inflammation support this hypothesis. For example, flagellin levels have been reported to be elevated in several murine models of intestinal inflammation, and flagellin can activate pro-inflammatory gene expression via TLR5 and the NLRC4 inflammasome [46,47,48,49]. Flagellin is also a dominant target of the immune system in Crohn’s disease [50]. ClpB and DnaK are chaperones that work together to solubilize and refold protein aggregates that can form during stress [51, 52]. They have also been shown to be important for motility and virulence in several different bacteria, and DnaK is immunogenic [53,54,55,56,57,58]. Furthermore, levels of DnaK and ClpB are increased in the intestinal bacterial proteomes of Crohn’s patients [59]. Molybdenum-cofactor-dependent metabolic pathways have been shown to be upregulated in inflammation-associated microbiota, and mutation of a gene important for the biosynthesis of molybdenum cofactor results in impaired fitness of several bacterial species under inflammatory conditions [60]. Notably, inhibition of molybdenum cofactor activity led to the abolishment of the expansion of Enterobacteriaceae and Deferribacteraceae (of which ASF457 M. schaedleri is a member) and amelioration of disease in dextran sulfate sodium-exposed mice [61]. Thus, our study suggests that in inflammatory environments, ASF457 M. schaedleri upregulates genes that are predicted to increase its fitness, virulence, and immunogenicity, allowing it to increase in abundance and induce continued inflammation. In future studies, it will be interesting to test whether co-colonization of Was−/− mice with H. bilis and ASF457 M. schaedleri is sufficient to cause intestinal inflammation.

ASF519 P. goldsteinii relative abundance was negatively correlated with LCN2, suggesting a possible protective role for this organism. Indeed, oral treatment of obese mice on a high-fat diet with P. goldsteinii led to decreased expression of IL-1β and increased expression of IL-10 in the colon as well as reduced intestinal permeability [39]. One mechanism for mediating these beneficial effects may be through the production of short-chain fatty acids [30]. Our metatranscriptomic analysis revealed that ASF519 P. goldsteinii expressed higher levels of superoxide dismutase and DNA starvation/stationary phase protection protein, which are important for adaptation to various types of stress, in WT mice compared to colitic Was−/− mice [62,63,64]. Thus, this bacterial species may have a relative fitness disadvantage in inflamed colons. Taken together, these data suggest that ASF519 P. goldsteinii abundance may decrease in inflammatory conditions due to relatively low expression of genes important for stress response, and the loss of this protective species may in turn worsen colitis.

Conclusions

In summary, our data support a model in which H. bilis serves as an initial trigger for inflammation in WASP-deficient mice, and this inflammatory environment supports the expansion of a second pathobiont, ASF457 M. schaedleri, which perpetuates inflammation. At the same time, ASF519 P. goldsteinii is not well-adapted for survival in the inflamed colon, and the loss of this protective species further exacerbates colitis. Our study highlights several key concepts that should be taken into consideration when designing future studies. First, the answer to the question of whether dysbiosis causes inflammation or inflammation causes dysbiosis is likely that either may be true depending on the timepoint. This emphasizes the importance of longitudinal studies that begin before the onset of inflammation. Second, microbes can have context-dependent effects, as illustrated by H. bilis’s differing effects on TH17 cells, ILC3s, and LCN2 depending on host genotype and inflammatory state. Thus, labeling a bacterial taxon broadly as “good” or “pathogenic” without studying its effects in multiple settings may be misleading. Finally, bacteria adapt to their environment by changing their gene expression, which affects how they interact with their host and with other microbes. While inferring functional capacity based on 16S rRNA sequencing or measuring functional potential based on metagenomic sequencing begins to address bacterial function, metatranscriptomic, metabolomic, and proteomic analyses are critical for assessing actual function and behavior in a given setting.

These concepts also have important implications for the design of microbial-based therapeutics. When developing the optimal consortium of bacteria to administer to patients, it will be important to consider how these bacteria will adapt to the inflamed environment they will be delivered into. Indeed, there is growing evidence supporting the importance of recipient characteristics, including inflammatory state, on fecal microbiota transplantation success [10]. Optimizing the intestinal environment to support the growth of beneficial bacteria and induce the desired transcriptional program within these bacteria may be critical to improving the efficacy of microbial-based therapies. Alternatively, beneficial bacteria could be genetically engineered to better survive in the inflamed colon and constitutively express genes that promote tissue repair and amelioration of inflammation.

Methods and Materials

Mice

Was−/− mice were generated as previously described [19]. WT and Was−/− mice (both on the 129 SvEv background) were maintained under specific pathogen-free (SPF), germ-free, or gnotobiotic conditions at Boston Children’s Hospital (BCH) as indicated. Mice were fed Prolab Isopro RMH 3000. Germ-free status was monitored by both 16S sequencing and culturing on a monthly basis. WT and Was−/− mice re-derived as Helicobacter-free were housed in Helicobacter-free conditions at Massachusetts General Hospital (MGH). Mice were between 3-6 months of age at the beginning of the gnotobiotic experiments. WT and Was−/− experimental groups were balanced by age and sex. For mice receiving H. bilis, the H. bilis Missouri strain was grown on tryptic soy agar plates with 5% sheep blood (Remel Laboratories, Lenexus, KS) and incubated under microaerobic conditions at 37 °C with a gas mixture of N2, Co2, and H2 (80:10:10). H. bilis was grown to an OD600 of 2, and 200 μL were delivered by orogastric gavage into each mouse twice, 2 days apart. Fecal samples were collected longitudinally to monitor for the onset of inflammation and changes in microbial composition. At 20 weeks following H. bilis gavage, mice were euthanized, and histology, spleen weights, colonic lamina propria immune cell populations, and mucosal microbiota were assessed. H. bilis colonization was confirmed via PCR analysis of fecal pellets. All experiments were conducted after approval from the BCH Institutional Animal Care and Use Committee and the subcommittee on Research Animal Care at Massachusetts General Hospital (MGH).

Isolation of cells from colonic lamina propria

Colons were harvested and placed in ice-cold phosphate-buffered saline (PBS). Fecal content was removed from the colon, and the colon was opened longitudinally, then cut into approximately 1 cm sections. The colon pieces were incubated in Hank’s balanced salt solution (HBSS, without Ca2+ and Mg2+) containing 0.5% fetal bovine serum (FBS), 10 mM EDTA, 10 mM HEPES, and 1mM DTT at 37°C with shaking for 20 min twice to remove the epithelial cell layer. After removal of the epithelial layer, tissues were washed in PBS, minced, and digested in HBSS buffer (with Ca2+ and Mg2+) containing 20% FBS, 1.5 mM CaCl2, and collagenase VIII (200 unit/ml) for 45 min at 37°C. The cells were then filtered and washed once in cold PBS.

Flow cytometry

For T cells, cells isolated from the colonic lamina propria were stimulated with PMA (100 ng/mL) and ionomycin (500 ng/mL) for 4–6 h. Dead cells were excluded using Fixable Viability Dye eFluor 506 (eBioscience). Surface staining was performed using antibodies to CD45 (30-F11), CD3 (145-2C11), and CD4 (GK1.5). Cells were fixed and permeabilized using the Cytofix/Cytoperm Fixation/Permeabilization Solution Kit (BD Biosciences), and intracellular cytokine staining was performed for IL-17A (TC11-18H10.1).

For ILC3s, cells isolated from the colonic lamina propria were rested in RPMI with 10% FBS and penicillin/streptomycin for 1–2 h. IL-7 and IL-23 (both from BioLegend, final concentration 20 ng/mL) were added, and cells were cultured overnight. They were then stimulated with PMA (100 ng/mL) and ionomycin (500 ng/mL) for 4–6 h. Dead cells were excluded using Fixable Viability Dye eFluor 506 (eBioscience). Surface staining was performed using antibodies to CD45 (30-F11), CD19 (1D3/CD19), CD3 (145-2C11), CD11b (M1/70), CD11c (N418), NK1.1 (PK136), Ly6C/G (RB6-8C5), Thy1.2 (30H12), CD127 (A7R34). Cells were fixed and permeabilized using the Cytofix/Cytoperm Fixation/Permeabilization Solution Kit (BD Biosciences), and intracellular cytokine staining was performed for IL-22 (Poly5164).

All antibodies were purchased from BioLegend. Samples were run on a BD LSRFortessa Cell Analyzer and analyzed using Flowjo software, version 10.

Histology

Tissue from the indicated areas of the colon were harvested and flushed with PBS. Tissue was fixed in 4% paraformaldehyde and embedded in paraffin. Sections were stained for H&E, and images were graded for severity of inflammation using a score that incorporates the following 4 parameters, each graded on a 4-point scale (0 = absent, 1 = mild, 2 = moderate, 3 = severe): mononuclear inflammation, crypt hyperplasia, epithelial injury, and neutrophilic inflammation/crypt abscesses.

Gut bacteria and mucus staining

Colon sections containing fecal material were fixed in Carnoy’s buffer to preserve the mucus layer and then were paraffin-embedded. 5-μm-thick sections were cut for staining. Bacteria were stained by fluorescence in situ hybridization using a Cy3-conjugated pan-specific EUB338 probe (5′- GCT GCC TCC CGT AGG AGT -3′), which covers more than 90% of the gut bacteria. The sections were counterstained with 4′,6-diamidino-2-phenylindole (DAPI), indicating the colonic epithelium and the lectin Ulex europaeus agglutinin I (UEA-1), indicating the mucus layer. Cells were imaged on a Zeiss LSM880 confocal Microscope. Original magnification 200× or 630×.

Fecal lipocalin-2 ELISA

Stool samples were collected by holding the mice and allowing them to defecate directly into sterile tubes. Stool was stored at – 80 °C. One to two stool pellets were weighed and placed in a fresh sterile tube, and 1 mL of PBS + 0.1% Tween20 was added. Tubes were clipped to a horizontal microtube attachment on a Vortex Genie 2 and vortexed at high speed for 5 min. 750 μL of stool homogenate were transferred to a fresh tube and vortexed for an additional 15 min. The samples were then centrifuged at 12,000 rpm for 10 min at 4 °C. The supernatant was transferred to a fresh tube and stored at − 20 °C. LCN2 was measured in the supernatant using the mouse lipocalin-2/NGAL DuoSet ELISA kit (R&D Systems) and normalized to stool weight.

Fecal DNA extraction and 16S library preparation

Fecal DNA was extracted using the DNeasy PowerSoil Kit for SPF samples. Due to the large number of samples in the ASF experiment, the similar but high throughput DNeasy PowerSoil HTP 96 Kit (384) was used. DNA from samples within the same study that were directly compared were extracted using the same kit. For mucosal samples, approximately 1-cm pieces of the proximal colon were stored in TRIzol Reagent at − 80 °C. After samples were thawed, they were rinsed in PBS and added to the bead tube of the DNeasy PowerSoil Kit (Qiagen). Sixty microliters of Solution C1 (Qiagen DNeasy PowerSoil Kit) were added to each tube, and the tubes were incubated at 70 °C for 10 min. Samples underwent bead beating in a Qiagen TissueLyser II at setting 30 for 2 min. Twenty microliters of proteinase K (Sigma) were added to each tube, and tubes were incubated at 65 °C for 30 min. Samples were then processed as per the DNeasy PowerSoil Kit (Qiagen) protocol beginning after the vortexing step.

The 16S rRNA V4 region was PCR-amplified using primers adapted from the 515F and 806R primers used by the Earth Microbiome Project and modified to include Illumina paired-end adaptors (forward: CTT TCC CTA CAC GAC GCT CTT CCG ATC TGT GCC AGC MGC CGC GGT AA; reverse GGA GTT CAG ACG TGT GCT CTT CCG ATC TGG ACT ACH VGG GTW TCT AAT) [65]. Nextera XT indices (Illumina) were attached to the 16S V4 amplicons during a second PCR step. Both PCR steps were performed using 5PRIME HotMasterMix (Quantabio). Cycling conditions for the first step were: 94°C for 3 min; 20 cycles of 94 °C for 45 s (28 cycles for mucosal samples), 50 °C for 60 s, and 72 °C for 90 s; then 72 °C for 10 min. For mucosal samples, two separate 16S rRNA PCR reactions were performed for each sample, and the products from the two independent first-round PCR reactions were combined as input for the second PCR round to attach indices. Cycling conditions for the second step were 94 °C for 3 min; 5 cycles of 94°C for 45 s, 65 °C for 60 s, and 72°C for 90 s; then 72 °C for 10 min. Amplicons were purified and normalized using the SequalPrep Normalization Plate Kit (Invitrogen). Paired-end sequencing was done on the Miseq platform using the 300-cycle V2 kit for stool samples and the 300-cycle Micro kit for mucosal samples. Sequencing of SPF fecal samples yielded a mean of 313,000 (standard deviation 95,000) read pairs per sample. Sequencing of ASF fecal samples yielded a mean of 18,000 (standard deviation 4,000) read pairs per sample. Sequencing of mucosal samples yielded a mean of 72,000 (standard deviation 15,000) read pairs per sample.

Analysis of 16S sequencing

The R package, dada2 (version 1.14.1), was used to process 16S amplicon sequence data to generate an amplicon sequence variant (ASV) table [66]. Specifically, reads were filtered and trimmed using the filterAndTrim command with options trimLeft=c(19,20), truncLen=c(150,150), maxN=0, maxEE=c(2,2), truncQ=2, rm.phix=TRUE. Sequence error models for forward and reverse reads were learned from the filtered, trimmed reads using the learnErrors command. Sequence errors were then corrected using the dada function, producing denoised reads that were then merged using the mergePairs function with options minOverlap=7, maxMismatch=1. For the mucosal bacteria dataset, reads were slightly shorter and therefore minOverlap=4 was used. An ASV table was then generated using the makeSequenceTable function. Chimeras were detected and removed using the removeBimeraDenovo function using the “consensus” method.

For samples from SPF mice, taxonomic assignment was performed using the dada2 functions assignTaxonomy and addSpecies using version 16 of the Ribosomal Database Project training set [67]. For samples from mice colonized with ASF with or without H. bilis, ASVs were identified by comparison to the corresponding 16S sequences found in reference genomes using the search_pcr command of USEARCH version 10 [68]. The NCBI accession numbers of the reference genomes used are as follows: NZ_CP019645.1 (Helicobacter bilis strain AAQJH); NZ_KB822565.1 (Clostridium sp. ASF356); NZ_KB822413.1 (Lactobacillus sp. ASF360); NZ_KB822402.1 (Lactobacillus murinus ASF361); NZ_KI530573.1 (Mucispirillum schaedleri ASF457); NZ_KB822471.1 (Eubacterium plexicaudatum ASF492); NZ_KI535318.1 (Firmicutes bacterium ASF500); NZ_RHJS01000002.1 (Schaedlerella arabinosiphila ASF502); NZ_KB822571.1 (Parabacteroides sp. ASF519).

The R package phyloseq version 1.30.0 was used for further analysis [69]. All beta diversity/principal coordinates analyses were performed after rarefaction to even sequencing depth. The rarefaction depth used was the minimum read depth among the samples involved in the analysis, unless this was less than 10,000, in which case 10,000 reads per sample were used.

Fecal RNA extraction and library preparation

For each mouse, a single fecal pellet was deposited into a Qiagen PowerBead glass 0.1 mm tube. 1 mL of Invitrogen TRIzol Reagent was added to each tube. The tubes were sealed and clipped to a horizontal microtube attachment on a Vortex Genie 2 and vortexed at high speed for 20 min. The tubes were centrifuged quickly, and 200 μL of chloroform was added. The tubes were then vortexed for 1 minute, allowed to homogenate at room temperature for 3 min, and then centrifuged at 4 °C for 15 min. The upper aqueous layer was carefully transferred to a 1.5-mL Eppendorf tube. A 1:1 ratio of 70% ethanol was added to each tube. The tubes were then sealed and vortexed for 1 minute followed by centrifugation at room temperature for 1 minute. The samples were then passed through Qiagen RNeasy Mini Kit spin columns, 600 μL at a time, and the flow-through was discarded. RNA was extracted as per the Qiagen RNeasy Mini Kit protocol, including the on-column DNase digestion step. 80% ethanol was used in place of RPE for the second RPE wash step. RNA was eluted by adding 50 μL of RNAse-free water to the center of each spin column membrane and incubating at room temperature for 5 min. The tubes were centrifuged at maximum speed at room temperature for 30 s. The captured eluate was then transferred to the center of each spin column membrane, and the spin columns were centrifuged at maximum speed for 2 min. The extracted RNA was quantified using the Invitrogen Qubit RNA HS Assay Kit, and the quality of the RNA was assessed using an Agilent RNA 6000 Pico Kit.

Fifty microliters of Beckman Coulter RNAClean XP beads were added to the 50 μl of extracted RNA for each sample. Samples were washed per Beckman Coulter’s wash protocol and eluted in 20 μl. Bacterial ribosomal RNA was removed using the Invitrogen RiboMinus Transcriptome Isolation Bacteria Kit, and the ribo-depleted RNA was eluted in 10 μl. Sequencing libraries were generated using the Illumina TruSeq Stranded Total RNA Library Prep Gold kit to ensure host ribosomal RNA depletion and mitochondrial RNA depletion. TruSeq RNA indexes were used to barcode samples.

cDNA libraries were washed using Beckman Coulter AMPure XP magnetic beads. Library quality and size verification were performed using the PerkinElmer LabChip GXII instrument with the DNA 1K Reagent Kit. Library concentrations were quantified using the Quant-iT dsDNA High Sensitivity Assay Kit using a Promega GloMax plate reader. Library molarity was calculated based on library peak size and concentration. Libraries were normalized to 2 nM using the PerkinElmer Zephyr G3 NGS Workstation and pooled together using the same volume across all normalized libraries into a 1.5-ml Eppendorf DNA tube. Pooled libraries were sequenced on an Illumina NextSeq500 high-output 75-bp single-read sequencing run. Between 3.4 million and 26.7 million 75-bp raw reads per sample were generated (median 16.9 million reads).

Analysis of metatranscriptomic sequencing

Raw sequence reads were deduplicated using the dedup function of samtools [70]. Deduplicated reads served as the input to both gene-level and pathway-level analyses.

For gene-based analyses, deduplicated reads were mapped using Bowtie2 [71] to an index containing the reference genomes of Helicobacter bilis and the ASF members (see Analysis of 16S Sequencing for accession numbers). The resulting mapped reads (BAM files) were then split into separate files according to species. Reads mapped per gene were counted with the Rsubread packag e[72], using the annotations (GFF files) provided with the reference assemblies. Reads mapping to ribosomal genes (annotated with gene_biotype = rRNA) were removed. All data associated with a given bacterial species were then grouped together, yielding the equivalent of a bulk RNA dataset for each species. Further differential expression analysis was performed using DESeq2 [73]. Principal components analysis was performed after applying the DESeq2 variance stabilization transformation.

For pathway-based analyses, ribosomal reads were removed from the deduplicated reads using the bbduk tool from the bbtools suite [74] with kmer length 27. First, reads mapping to ribosomal small subunit sequences in the SILVA SSU database version 132 [75] were removed. Reads mapping to large subunit sequences in SILVA LSU database version 132 were then removed. Prior to use, these databases were modified to replace nucleotides U with T. The filtered reads were then analyzed using a beta version of HUMAnN3 [76], yielding pathway abundances normalized as copies per million (CPM). For the purposes of principal coordinates analysis, high-variance pathways (GO terms) were identified as follows. Pathways were filtered to remove those present in 3 or fewer samples. Pathway abundances were log-transformed (using a pseudocount of 0.1 CPM). It was noted that the mean/variance relationship of the resulting transformed values was strongly confounded by pathway prevalence (that is, by the number of samples in which each pathway was detected). This confounding effect was taken into account by grouping pathways according to the number of samples with each pathway detected and performing a linear regression in which the independent variable was the mean log-transformed CPM and the dependent variable was the corresponding standard deviation. The resulting residuals were then z-scored in order to normalize across all pathways. Pathways with high z-scores were considered to have high variance, taking into account overall expression and pathway prevalence. Principal component analysis (PCA) of GO term expression was performed using the log-transformed CPM values of the 30 highest variance GO terms. Clustering of samples according to genotype was assessed using PERMANOVA as implemented by the adonis function of the vegan R package [77] using Euclidean distance.

Statistical analyses

Statistical tests appropriate for each type of data are indicated in the figure legends and were performed using R (R Core Team 2021) [78] or GraphPad Prism (version 9.1.2). For box-and-whisker plots, boxes show 25th, 50th, and 75th percentiles, and whiskers include all data within 1.5 box lengths of the box. Outliers beyond the whiskers are shown as points. Correlations between log-transformed LCN2 levels and bacterial relative abundances were assessed using the Pearson correlation test in cases when one sample per mouse was used. When multiple samples per mouse were involved, these correlations were tested by fitting linear mixed-effects models using the lme4 R package [79] with random mouse effects.

Availability of data and materials

The datasets generated during and/or analyzed during the current study are available at the following NCBI BioProject accession numbers: PRJNA729789, PRJNA729739, PRJNA729746, PRJNA729753, PRJNA729783.

Abbreviations

AIEC:

adherent-invasive Escherichia coli

ASF:

Altered Schaedler flora

ASV:

Amplicon sequence variant

CPM:

Copies per million

FBS:

Fetal bovine serum

HET:

Heterozygous

IBD:

Inflammatory bowel disease

ILC3:

Group 3 innate lymphoid cells

LCN2:

Lipocalin-2

PBS:

Phosphate-buffered saline

PCA:

Principal component analysis

SPF:

Specific pathogen-free

TH17:

T helper 17

Treg :

Regulatory T cells

UEA-1:

Ulex europaeus agglutinin I

WASP:

Wiskott-Aldrich syndrome protein

WT:

Wild-type

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Acknowledgements

We would like to thank Alejandro Chavez for thoughtful discussions and critical reading of the manuscript. We would also like to thank the Harvard University Biopolymers Facility and Weill Cornell Medicine Genomics Resources Core Facility for next-generation sequencing, the Weill Cornell Medicine Microbiome Core Lab for meta-transcriptomic library preparation and sequencing, and the Beth Israel Deaconess Medical Center Histology Core Facility for processing of histology samples.

Funding

A.M.T. is the recipient of a Crohn’s and Colitis Foundation (CCF) Research Fellowship Award (527125) and Boston Children’s Hospital Fred Lovejoy House-staff Research and Education Award. J.A.G. is supported by the National Institute of Diabetes and Digestive and Kidney Diseases DK106311 and a CCF Career Development Award (352644). G.G.P. is supported by the Jill Roberts Institute at Weill Cornell Medicine. S.B.S. is supported by NIH Grants P30DK034854, the Helmsley Charitable Trust, the Wolpow Family Chair in IBD Treatment and Research, and the Translational Investigator Service at Boston Children’s Hospital.

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Authors and Affiliations

  1. Division of Gastroenterology, Hepatology and Nutrition, Boston Children’s Hospital, Boston, MA, USA

    Amy M. Tsou, Jeremy A. Goettel, Bin Bao, Amlan Biswas, Yu Hui Kang, Naresh S. Redhu, Kaiyue Peng, Jeffrey Saltzman, Ryan Kelly, Jordan Gringauz, Jared Barends, Sandra M. Frei, Rohini Emani, Bruce H. Horwitz & Scott B. Snapper

  2. Harvard Medical School, Boston, MA, USA

    Amy M. Tsou, Jeremy A. Goettel, Bin Bao, Amlan Biswas, Yu Hui Kang, Naresh S. Redhu, Sandra M. Frei, Rohini Emani, Jonathan N. Glickman, Bruce H. Horwitz & Scott B. Snapper

  3. Jill Roberts Institute for Research in Inflammatory Bowel Disease, Weill Cornell Medical College, New York, NY, USA

    Amy M. Tsou, Gregory G. Putzel & Mai Hatazaki

  4. Division of Pediatric Gastroenterology and Nutrition, Weill Cornell Medical College, New York, NY, USA

    Amy M. Tsou

  5. Division of Gastroenterology, Hepatology, and Nutrition, Department of Medicine, Vanderbilt University Medical Center, Nashville, TN, USA

    Jeremy A. Goettel

  6. Department of Gastroenterology, Children’s Hospital of Fudan University, Shanghai, China

    Kaiyue Peng & Ying Huang

  7. Division of Comparative Medicine, Massachusetts Institute of Technology, Cambridge, MA, USA

    Zeli Shen & James G. Fox

  8. Department of Pathology, Beth Israel Deaconess Medical Center, Boston, MA, USA

    Jonathan N. Glickman

  9. Division of Emergency Medicine, Boston Children’s Hospital, Boston, MA, USA

    Bruce H. Horwitz

  10. Division of Gastroenterology, Brigham and Women’s Hospital, Boston, MA, USA

    Scott B. Snapper

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  1. Amy M. Tsou
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Contributions

Conceived and designed the experiments: A.M.T., J.A.G, and S.B.S. Performed experiments: A.M.T., J.A.G., B.B., A.B., Y.H.K., N.S.R., K.P., J.S., R.K., J.G., J.B., M.H., S.M.F, R.E. Analyzed data: A.M.T, J.A.G., B.B., A.B, G.P., and J.N.G. Provided reagents: Y.H., Z.S., and J.G.F. Wrote the manuscript: A.M.T. Edited the manuscript: J.A.G., B.H.H., and S.B.S. All authors approved the final version of the manuscript.

Corresponding authors

Correspondence to Amy M. Tsou or Scott B. Snapper.

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Ethics approval and consent to participate

This study was approved by the Boston Children’s Hospital (BCH) Institutional Animal Care and Use Committee and the subcommittee on Research Animal Care at Massachusetts General Hospital (MGH). The review was conducted in accordance with the standards outlined in the National Research Council’s Guide for the Care and Use of Laboratory Animals and BCH’s Animal Welfare Assurance.

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Not applicable

Competing interests

S.B.S. is supported by grants or in-kind contributions from Pfizer, Novartis, Takeda, and Regeneron. He is on the scientific advisory boards of Pfizer, Janssen, IFM Therapeutics, Merck, Lycera, Inc., Celgene, Lilly, Pandion Therapeutics, and Applied Molecular Transport. He has consulted for Amgen, Hoffman La-Roche.

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Supplementary Information

Additional file 1: Fig. S1.

WASP deficiency results in altered composition of the fecal microbiota. Fecal microbial composition of Was-/- (n=5) and WT (n=3) mice raised under SPF conditions with weekly bedding exchanges was analyzed monthly between 4 and 20 weeks of age by 16S rRNA gene sequencing. (A) Microbial relative abundances at the phylum level. (B) Relative abundance of the phylum Deferribacteres at each timepoint by genotype. Statistics performed using the DESeq2 R package and adjusted for multiple taxa comparisons. * p < 0.05, ** p < 0.01, *** p < 0.001. Fig. S2. Establishing a reductionist model to study the role of the microbiota in the development of intestinal inflammation. (A) Experimental design. (B) Fecal microbial composition of donor mice harboring the ASF consortium and recipient ex-germ-free mice after 2 months of co-housing (females) or bedding exchanges (males) with donors. Composition assessed based on 16S rRNA sequencing. (C) Quantitative histological colitis scores 20 weeks after gavage with H. bilis in the cecum, proximal colon, and distal colon. (D) Gating strategy for IL-17A+ CD4 T cells. (E) Gating strategy for IL-22+ ILC3s. Lin includes CD3, CD19, CD11b, CD11c, NK1.1, Ly6C, Ly6G. Fig. S3. Correlations between intestinal inflammation and fecal microbial composition. (A-B) Germ free WT/HET (n=11) and Was-/- (n=8) mice colonized with the ASF community but not gavaged with H. bilis served as a control group. Fecal LCN2 (A) and absence of H. bilis (B) were monitored serially. (C) In mice that received H. bilis, H. bilis relative abundance is shown based on whether the mouse was in a cage that contained both genotypes (co-housed) or only one genotype (not co-housed). (D-I) Correlations between log-transformed fecal LCN2 and relative abundances of the indicated ASF members in mice of the indicated genotype colonized with ASF and H. bilis for all timepoints. Tests for linear dependence of log-transformed LCN2 on the relative abundance of each bacterial species was done using a linear mixed effects model, taking into account a mouse-specific random effect. Wilcoxon rank sum test was used for (A) and (C). * p < 0.05, *** p < 0.001. Fig. S4. Correlations between intestinal inflammation and mucosal microbial composition. (A-H) Germ free WT/HET (n=17) and Was-/- (n=13) mice were colonized with the ASF community and then gavaged with H. bilis. The mucosal-associated microbiota was assessed at 20 weeks post infection. Pearson correlations between log-transformed fecal LCN2 and mucosal relative abundances of the indicated ASF members are shown within mice of the indicated genotypes. Fig. S5. Metatranscriptomic analysis of ASF members in inflamed and uninflamed states. Germ-free WT/HET and Was-/- mice were colonized with the ASF community and a subset were gavaged with H. bilis. Feces from 3 mice of each genotype/microbiota combination (WT/HET or Was-/- with or without H. bilis) at 1.5 weeks and 15 weeks after H. bilis gavage were subjected to bacterial metatranscriptomic sequencing. (A-J) Volcano plots of corrected p-values vs log2(FC) for genes expressed by the indicated bacteria at 15 weeks after H. bilis gavage, except for (A-C), which are from 1.5 weeks after H. bilis infection. Plots compare gene expression of the specified bacteria between host genotypes within the ASF-only group or the ASF + H. bilis group as indicated. Dots on the right side of each plot represent genes that are more highly expressed in Was-/-, and dots on the left side indicate genes that are more highly expressed in WT/HET. Red dots represent genes that are significantly differentially expressed as determined by the DESeq2 Wald test.

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Tsou, A.M., Goettel, J.A., Bao, B. et al. Utilizing a reductionist model to study host-microbe interactions in intestinal inflammation. Microbiome 9, 215 (2021). https://doi.org/10.1186/s40168-021-01161-3

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Microbiome

ISSN: 2049-2618

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