- Open Access
Bradymonabacteria, a novel bacterial predator group with versatile survival strategies in saline environments
Microbiome volume 8, Article number: 126 (2020)
Bacterial predation is an important selective force in microbial community structure and dynamics. However, only a limited number of predatory bacteria have been reported, and their predatory strategies and evolutionary adaptations remain elusive. We recently isolated a novel group of bacterial predators, Bradymonabacteria, representative of the novel order Bradymonadales in δ-Proteobacteria. Compared with those of other bacterial predators (e.g., Myxococcales and Bdellovibrionales), the predatory and living strategies of Bradymonadales are still largely unknown.
Based on individual coculture of Bradymonabacteria with 281 prey bacteria, Bradymonabacteria preyed on diverse bacteria but had a high preference for Bacteroidetes. Genomic analysis of 13 recently sequenced Bradymonabacteria indicated that these bacteria had conspicuous metabolic deficiencies, but they could synthesize many polymers, such as polyphosphate and polyhydroxyalkanoates. Dual transcriptome analysis of cocultures of Bradymonabacteria and prey suggested a potential contact-dependent predation mechanism. Comparative genomic analysis with 24 other bacterial predators indicated that Bradymonabacteria had different predatory and living strategies. Furthermore, we identified Bradymonadales from 1552 publicly available 16S rRNA amplicon sequencing samples, indicating that Bradymonadales was widely distributed and highly abundant in saline environments. Phylogenetic analysis showed that there may be six subgroups in this order; each subgroup occupied a different habitat.
Bradymonabacteria have unique living strategies that are transitional between the “obligate” and the so-called facultative predators. Thus, we propose a framework to categorize the current bacterial predators into 3 groups: (i) obligate predators (completely prey-dependent), (ii) facultative predators (facultatively prey-dependent), and (iii) opportunistic predators (prey-independent). Our findings provide an ecological and evolutionary framework for Bradymonadales and highlight their potential ecological roles in saline environments.
Bacterial predators have been proposed as an indispensable selective force in bacterial communities [1,2,3,4]. Predation by bacteria can release nutrients  and affect biogeochemical cycling. In contrast to phages, bacterial predators do not need to be present in high concentrations to drive significant bacterial mortality in the environment [6, 7]. In addition, bacterial predators have higher prey-killing efficiency in low-nutrient medium than phages . However, these studies have mostly been based on Bdellovibrio and like organisms (BALOs), and little is known of the ecological roles of other bacterial predators.
Predatory bacteria are classified into two categories, obligate or facultative predators, based on their prey-independent or prey-dependent living strategies . Obligate predators include several genera collectively known as BALOs . These predatory bacteria can attack their prey by penetrating the cell wall , dwelling in the periplasm, and then killing their host . Therefore, their lifestyle depends on the presence of their prey in the natural environment, and BALOs lose viability within several hours if the prey is not available [8, 13, 14]. Facultative predators also include several genera , such as Myxococcus, Lysobacter, and Herpetosiphon . These predators kill their prey by secreting antimicrobial substances into the surrounding environment [9, 16]. In general, the so-called facultative predators have been considered to be those that can be maintained as pure bacterial cultures and be free living without their prey in natural environments. However, due to the lack of additional types of predators, no assessment could be made with respect to how dependent they were on their prey. Furthermore, whether there is a transitional type between obligate and so-called facultative predators is unclear.
Bradymonabacteria are representative of the novel order Bradymonadales, which are phylogenetically located in the δ-Proteobacteria . The first type species of Bradymonadales, Bradymonas sediminis FA350T, was isolated in 2015 . To date, 9 strains within the Bradymonadales have been isolated and found to belong to 7 candidate novel species; these Bradymonabacteria are bacterial predators . Interestingly, the phylum Proteobacteria contains three orders of predatory bacteria. Among them, Myxococcales and Bradymonadales belong to δ-Proteobacteria, while Bdellovibrionales were classified as Oligoflexia in 2017 . Myxococcales and Bdellovibrionales are so-called facultative and obligate predators, respectively. Additionally, they have different distribution patterns in the environment. Myxococcales are mainly found in soil and sediment niches [20, 21], while Bdellovibrionales are aquatic. However, how Bradymonadales adapt to predatory lifestyles and whether they have specific living strategies or ecological importance remain largely unknown.
Here, we analyzed the predation range of Bradymonadales on diverse bacteria and their predatory morphological and physiological characteristics. By using comparative genomic analysis of Bradymonadales and other predatory bacteria, we revealed the genetic and metabolic potential of this group. To assess the diversity and frequency of occurrence of the various ribotypes of known predators (Bradymonadales, Myxococcales, and Bdellovibrionales) on a global scale, we surveyed published 16S rRNA gene amplicon datasets from a number of ecosystems representing a broad range of geographic locations, climatic zones, and salinities. Our study provides an ecological and evolutionary framework for Bradymonadales and highlights their potential ecological roles in predation.
Bradymonabacteria are efficient predators of diverse prey bacteria
In total, 9 strains of bacteria in the novel order Bradymonadales were isolated using the enrichment culture method . Among these strains, eight strains were isolated from costal sediment sampled in Weihai, China, while strain YN101 was isolated from a Gaodao saltern (36° 54′ N, 122° 14′ E) in Weihai, China. Strains FA350T [17, 18] and B210T  are the two type strains for different genera of Bradymonadales. Both these type strains were used to investigate the predator-prey range of Bradymonabacteria. A total of 281 isolated bacteria were cocultured with Bradymonabacteria FA350T [17, 18] or B210T  as lawns in individual Petri dishes (Fig. 1a, Table S1). Zones of predation were measured (Fig. 1b), and the results showed that the Bradymonabacteria preyed on diverse bacteria but showed a strong preference for Bacteroidetes (90% of tested bacteria could be preyed on) and Proteobacteria (71% of tested bacteria could be preyed on) (Fig. 1c). Predation on bacteria in the orders Flavobacteriales, Caulobacterales, Propionibacteriales, and Pseudomonadales was broadly distributed, with a mean predation percentage greater than 90%, while predation of Micrococcales and Enterobacteriales was less efficient.
Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) analyses were performed to understand the mechanism of predation of strain FA350T on the subcellular level. Lysis of the prey cells (Fig. 2a) was detected near strain FA350T in both the TEM and SEM analyses (Fig. 2). Strain FA350T was found to have pili (Fig. 2b, g) and outer membrane vesicle (OMV)-like structures (Fig. 2d, e, f, h). In addition, FA350T cells contained intracellular particles with low electron density (Fig. 2b, c, d, f), which were shown to contain polyhydroxyalkanoates (PHAs) by Nile blue A staining. FA350T cells also contained several electron-dense (black) spots (Fig. 2b, c, d, f), which indicated the presence of intracellular polyphosphate granules . Both of these particle types significantly accumulated during predation (Fig. 2).
Bradymonabacteria are polyauxotrophs
To explore the metabolic capabilities and predation mechanism of this novel group, we analyzed 13 genomes of Bradymonadales (9 high-quality genomes sequenced from cultured strains and 4 reconstructed from published studies ). The genome size of Bradymonabacteria ranged from 5.0 to 8.0 Mb (Fig. S1a). Average nucleotide identity (ANI) analysis of the 9 cultured strains of Bradymonadales revealed 7 different species  (Fig. S1b). Other general features of the genomes are described in the Supplementary Materials (Supplementary Materials Results and Fig. S1a).
Almost all strains (except FA350T) possessed a minimal pentose phosphate pathway, which lacked key steps for the synthesis of ribose 5-phosphate (Fig. 3, Table S2) . Most of the bradymonabacterial genomes lacked key enzymes for pyrimidine synthesis, such as aspartate carbamoyltransferase, which catalyzes the first step in the pyrimidine biosynthetic pathway. All genomes lacked the complete purine de novo pathway; they were missing the phosphoribosylaminoimidazole carboxylase catalytic subunit or even the whole pathway.
In addition to this auxotrophy in the synthesis of pentose and nucleotides, all the genomes lacked complete pathways for the synthesis of many amino acids, such as serine, methionine, valine, leucine, isoleucine, histidine, tryptophan, tyrosine, and phenylalanine (Fig. 3). For example, all the genomes encoded a potential D-3-phosphoglycerate dehydrogenase for the conversion of glycerate-3P into 3-phosphonooxypyruvate for amino-acid synthesis (Fig. 3). However, in all members of Bradymonabacteria, this pathway appeared to be blocked at the subsequent step because of the absence of phosphoserine aminotransferase, although Bradymonabacteria could continue with subsequent pathways to complete the biosynthesis of cysteine and glycine. Additionally, many cofactors and vitamins that promote bacterial growth , such as biotin, thiamin, ubiquinone, VB12, and VB6, could not be synthesized by the de novo pathway in almost all the genomes. Notably, all the genomes had an incomplete pathway for type II fatty acid biosynthesis, lacking the key enzymes 3-oxoacyl-[acyl-carrier-protein] synthase I/II (FabB/F) and enoyl-[acyl-carrier-protein] reductase (FabI/L).
Dual transcriptome analysis of the potential predation mechanism of Bradymonabacteria
To further determine the genes involved in predation, we performed dual transcriptome analysis of Bradymonas sediminis FA350T with and without preying on Algoriphagus marinus am2 (Fig. S2). As with obligate predators, one way that Bradymonabacteria kill their prey bacteria is likely by using contact-dependent mechanisms. Here, the bradymonabacterial genomes possessed complete type IV pili (T4P) (Fig. 3), and the attached areas showed more type IV pili than the unattached areas (SEM, Fig. 2g, h). The dual-transcriptome analysis showed that genes encoding the type IV pili twitching motility protein PilT (DN745_17255) were significantly upregulated during predation (Fig. S3), suggesting that these genes may be involved in predation. Bradymonabacteria also had T4b pilins showing homology to those in Bdellovibrio bacteriovorus HD100, in which T4b pilins are necessary for predation [28, 29] (Fig. S4), so T4b pilins may also participate in regulating predation in Bradymonabacteria. In addition, this group of bacteria had type II and type III secretion systems (the YscRSTUV proteins that form a membrane-embedded complex known as the “export apparatus” ). The dual-transcriptome analysis also supported the prediction that genes encoding the type III secretion system inner-membrane protein complex (DN745_01900, DN745_10315, DN745_17280, DN745_03325, and DN745_00480) were significantly upregulated during predation (Fig. S3), implying that these genes may also be involved in predation.
Another way that Bradymonabacteria kill their prey bacteria is likely by secreting antimicrobial substances into the surrounding environment. As in most facultative bacterial predators, a few potential antimicrobial clusters for secondary metabolite synthesis, such as Lasso peptide , were identified in almost all genomes of Bradymonabacteria (Fig. 3). Genes involved in outer membrane vesicle (OMV) biosynthesis were also detected in most genomes, such as ompA (cell envelope biogenesis protein), envC (Murein hydrolase activator), and tolR (envelope stability) . Vesicle membrane-related genes (DN745_03865, DN745_02930, and DN745_07125) were significantly upregulated during predation (Table S4, Fig. S3). However, the fermentation supernatant of Bradymonabacteria showed no antibacterial activity.
Bradymonabacteria are novel predators different from the so-called obligate or facultative predators
Comparative genomic analysis with other bacterial predators was performed to explore whether Bradymonabacteria have a unique living strategy. Two-way cluster analysis showed that bradymonabacterial genomes contained features different from those of either obligate or facultative predators, which were phylogenetically located in a different branch (Fig. 4). The specific multiple metabolic deficiencies of Bradymonabacteria had some similarities to those of most obligate predators. For example, both Bradymonabacteria and obligate predators possessed a minimal pentose phosphate pathway, lacked key enzymes for pyrimidine synthesis, and lacked complete pathways for the synthesis of many amino acids, cofactors, and vitamins (Fig. 4). However, Bradymonabacteria with multiple auxotrophies could grow on common media (such as marine agar medium), though at a low growth rate , unlike obligate predators.
Unlike most obligate predators, the polyphosphate accumulation pathway, containing a pair of genes (polyphosphate kinase and exopolyphosphatase) associated with both polyphosphate formation and degradation , was present in most Bradymonabacteria (Fig. 4). Polyphosphate accumulation was also detected in FA350T cells during predation (Fig. 2). In contrast to most of the other predator genomes, potential PHA synthesis from β-oxidation of fatty acids  was observed in most bradymonabacterial genomes (Fig. 3). In the present study, TEM analysis showed that strain FA350T could significantly accumulate PHAs during predation observed in co-culture with pure culture (Fig. 2). Despite their incomplete fatty acid biosynthetic pathway, all Bradymonabacteria had a high copy number of long-chain fatty acid transporters (fadL) compared to those of other predators, allowing them to gather fatty acids from the environment (Fig. 4). In addition, genes associated with alkane synthesis, which is important for maintaining cell membrane integrity and adapting to cold environments , were present in most genomes of Bradymonabacteria (Figs. 3 and 4). Thus, we proposed that Bradymonabacteria could be categorized as novel predators different from so-called obligate or facultative predators (Table 1).
Bradymonadales are mainly distributed in saline environments with high diversity
To evaluate the global prevalence of the Bradymonadales order, we surveyed recently published 16S rRNA gene amplicon studies that provided high taxonomic resolution along with relative sequence abundances. The 16S rRNA gene amplicons from 1552 samples were grouped into eight types of environments (Fig. 5a and Table S5). A total of 811 samples were from inland environments, while others were from marine environments, with each biotope showing a somewhat different microbial community (Fig. 5b). Based on the alpha diversity analysis, marine sediment and soil biotopes harbored more OTUs compared with other biotopes (Fig. S5). Bradymonabacteria was detected in 348 of 741 marine samples (relative abundance > 0.01%) but only 20 of 544 soil samples (Fig. 5a). All samples were sorted into an ordination diagram based on the similarity of communities (Fig. 5b). Saline biotopes were clearly separated from nonsaline biotopes (Fig. S6), suggesting that salinity was a significant factor in shaping microbial communities. For each biotope, the relative abundance of Bradymonadales in the saline environments (i.e., seawater and saline lake sediment) was significantly higher than that in the nonsaline environments (i.e., nonsaline soil and nonsaline water) (P ≤ 0.0001, Fig. 5c). The distribution analysis was consistent with the genomic feature analysis (Fig. 2), in which several genes encoding sodium symporters and Na+/H+ antiporters were found in the genomes, suggesting a beneficial effect of salinity on Bradymonabacteria.
In addition, we compared the relative abundance of Bradymonadales with those of two orders of well-known predatory bacteria, Bdellovibrionales and Myxococcales [12, 37, 38]. We found that Myxococcales and Bdellovibrionales were also globally distributed (Fig. S7); however, Myxococcales were more commonly distributed in soil and sediment environments, while Bdellovibrionales were more likely to be found in freshwater and seawater (Fig. S7). The total relative abundances of Bradymonadales, Bdellovibrionales, and Myxococcales ranged from 0.7 to 6.4% of the total prokaryotic microbes in all 1552 samples (Fig. S8a). The mean relative abundance of Bradymonadales (0.5%) was similar to that of Bdellovibrionales (0.6%) when both were detected in environmental samples (Fig. S8b). In contrast, Bradymonadales was one of the most abundant known predatory bacteria in saline lake sediment and saline lake water (Fig. S8c).
To further determine how salinity affected the relative abundance of Bradymonadales, we used the Gaodao multipond salterns as a model and applied 16S rRNA gene amplicon, fluorescence in situ hybridization (FISH) (Tables S6 and S7), and real-time PCR analyses (Figs. S8d and S9). The results showed that Bradymonadales appeared in all the tested multipond saltern datasets, accounting for an average of 0.74% of all bacterial sequences and more than 1.0% relative abundance within the range of 80 and 265 g/L salinity (Fig. S8d), significantly higher than those of Bdellovibrionales and Myxococcales. Bradymonadales may exhibit different correlations with prey at different abundances, a possibility that will require further study. In addition, fluorescence in situ hybridization (FISH) (Tables S6 and S7) and real-time PCR experiments were performed, and the results showed a relative cell abundance of Bradymonadales of up to 0.6% and a gene copy numbers ratio as high as 1.96% in sediments with salinity 80 g/L (Fig. S9). These findings support those of the global analysis (Fig. S8c) and suggest that Bradymonadales may be a dominant bacterial predator in some specific saline environments.
To explore the diversity and distinct evolution of bradymonabacterial subgroups in different biotopes, we performed a phylogenetic analysis of nearly full-length 16S rRNA gene sequences of diverse origin by maximum likelihood inference (Table S6). A total of 187 OTUs were detected and found to form six sequence clusters (Fig. 6a). Almost 87.2% of the representative sequences originated from saline biotopes (such as seawater, marine sediments, salterns, corals, and saline lakes). Since bradymonabacterial subgroups may be selectively distributed in local biotopes, we investigated the relative abundance of each subgroup throughout the 127 representative samples in which the relative abundance of Bradymonadales was above 1% of total 16S rRNA gene reads (Fig. 6b). Five of the 6 bradymonabacterial subgroups showed significantly higher abundance in saline environments. Cluster-2 and cluster-6 were mainly observed in seawater biotopes, whereas cluster-3 was mainly observed in marine sediment and saline lake sediment (Fig. 6b), consistent with the environments of the cultured strains. Cluster-1 and cluster-4 were both detected in marine sediment and seawater biotopes. Cluster-5 lineages tended to occur in both freshwater and seawater biotopes (Fig. 6b).
In all ecosystems, predation is an important interaction among living organisms. Bacterial predators are proposed to play an important role in controlling and shaping bacterial populations in diverse environments [3, 39]. However, despite their ecological importance, only a few examples of predatory bacteria have been studied in depth. Recently, many predatory bacteria from various phyla have been isolated from different environments; however, most of their predatory lifestyle strategies and adaptations remain unclear. This study systematically analyzed the predatory lifestyle adaptations, global distribution, and diversity of Bradymonadales; highlighted the ecological role of Bradymonadales; and provided a framework for the categorization of the known predatory bacteria.
In our study, based on comparative genomic and physiological analyses, Bradymonabacteria were identified as a novel group of bacterial predators with versatile survival strategies that are transitional types between “obligate” predators and the so-called facultative predators (Table 1). Similar to the obligate predators, Bradymonabacteria have multiple metabolic deficiencies. Their incomplete pathways might be important for prey-dependent growth, as the precursor compounds could be acquired from predation. In addition, the loss of genes in the fatty acid biosynthetic pathway was notable, because fatty acids are integral components of the cellular membrane, and their synthesis is considered to be a housekeeping function of cells . Thus, these organisms may incorporate exogenous fatty acids from prey bacteria into their membrane phospholipids using their high copy number of long-chain fatty acid transport proteins  (Fig. 3). Unlike the currently called “facultative” predators, the multiple gene loss observed in Bradymonabacteria may render them more dependent on prey for their lost metabolic functions and could also provide a selective advantage by conserving predators’ limited resources . However, the sequenced genomes of Bradymonabacteria were surprisingly large (5.0 to 8.0 Mb, Fig. S1a), suggesting that Bradymonabacteria are far from obligate parasites, with seemingly none of the reductive evolution that results from a parasitic lifestyle in bacteria such as Mycobacterium leprae . The large size of their genomes may be indicative of the vast range of genes required for Bradymonabacteria to both effectively tolerate the absence of prey and carry out predation.
In contrast to most predators, Bradymonabacteria can synthesize many nutrient polymers, such as polyphosphate, PHA, and alkane molecules. Exopolyphosphatase catalyzes the hydrolysis of terminal phosphate residues from polyphosphate chains, accompanying the production of ATP and thus playing a role in the production of energy . Bradymonadales cells may accumulate polyphosphate in the phosphate-rich zone, using it as an energy source . Meanwhile, PHA granules are synthesized as sinks of excess carbon and are used as carbon and energy reserves in starvation conditions . Under nutrient starvation, maintenance energy and free amino acids can be provided by endogenous substrates such as PHAs and polyphosphate [47, 48]; this ability may be an important feature for the survival of Bradymonabacteria during intervals without predation. This feature is interesting among bacterial predators, as it is commonly found in animal predation. For example, the bear can store fat in its body to ensure that it will survive the long winter. In addition, Bradymonabacteria may also synthesize alkanes to maintain cell membrane integrity  and complement its poor fatty acid synthesis ability. Thus, these multiple auxotrophies and ability to synthesize nutrient polymers confer on Bradymonabacteria a versatile survival strategy for natural environments, which contrasts with that of the currently known “obligate” or “facultative” predators.
As bacterial predators, Bradymonabacteria have developed a wide range of mechanisms to attack their prey. Although genes involved in OMV-like biosynthesis were detected in most genomes, the fermentation supernatant of Bradymonabacteria showed no antibacterial activity, suggesting that bradymonabacterial predation depends on the cell contact, which is different from that of the currently known facultative predators. Contact-dependent predation mechanisms allow predators to attach to the prey and then carry out predation. This prey-dependent process has a relatively low energy cost and could prevent secretory virulence factors from being diluted by the surrounding environment . Bradymonabacteria also has T4P, which could pull adherent bacteria into close association with other bacteria . T4P could also transport bound substrates such as DNA  into the periplasm and export exoproteins across the outer membrane . Contact-dependent type III secretion systems have also been found in Bradymonabacteria and are reported to be capable of moving virulence factors across bacterial outer membranes and directly across the host cell membrane into the cytoplasm of a host cell . However, no reports have indicated that the type III secretion system is involved in direct combat between bacteria. Whether type III secretory complexes could penetrate the bacterial cell wall is unknown. Further gene knockout experiments and systematic TEM analysis should be performed to identify whether and how the type III secretion system works during predation.
Our biogeographic analysis suggested that Bradymonabacteria are mainly distributed in saline environments, and some other studies have also detected Bradymonadales in hypersaline soda lake sediments , suggesting that saline environments could be enriched in these bacteria. Our genome analysis also showed that Bradymonadales had many genes encoding sodium symporters and Na+/H+ antiporters to maintain osmotic pressure in saline environments. These findings supported the global analysis (Figs. 5 and S8c), suggesting that Bradymonadales might be a dominant bacterial predator in some specific saline environments compared with Bdellovibrionales and Myxococcales. The analysis of the complex intragroup phylogeny of the 6 subgroups of Bradymonabacteria revealed that distinct evolutionary bradymonabacterial subgroups had arisen in different biotopes, suggesting the occurrence of adaptive evolution specific to each habitat. Patterns related to salinity status also suggest that most Bradymonadales are halophiles .
Bradymonabacteria had a very high predation efficiency on bacteria within the phylum Bacteroidetes. Members of the phylum Bacteroidetes are one the most abundant groups of bacteria in the ocean . Thus, a high predation efficiency on Bacteroidetes may indicate that Bradymonabacteria has important roles in regulating Bacteroidetes communities in oceans. Furthermore, Bradymonabacteria had a high predation efficiency on Flavobacteria and Proteobacteria, some of which are commensal bacteria in fish , suggesting that Bradymonabacteria may be involved in fish microbiome dysbiosis. In addition, Bradymonadales were detected in coral samples, as Bradymonabacteria have a wide range of prey, including the coral pathogen Vibrio harveyi, suggesting that Bradymonabacteria may protect coral hosts by consuming potential pathogens . The exact ecological roles of this group in different environments should be determined in further studies.
The unique metabolic pathways of Bradymonabacteria, which include conspicuous metabolic deficiencies similar to those of obligate predators but with a more effective starvation stress response mechanism, provide these bacteria with transitional survival models between “obligate” and so-called facultative predators. We suggest that Bradymonabacteria, as facultative prey-dependent predators, can be renamed as facultative predators. In addition, the currently used “facultative” predators term can be replaced by opportunistic predators, which are prey-independent predators. Thus, we propose a framework to categorize the current bacterial predators into 3 groups: (i) obligate predators (completely prey-dependent), such as most of the BALOs; (ii) facultative predators (facultatively prey-dependent), such as the Bradymonabacteria cultured in the present study; and (iii) opportunistic predators (prey-independent), such as Myxobacteria and Lysobacter sp. (Table 1). This categorization replaces the currently known “facultative” predators with opportunistic predators and will be helpful for further study of the different ecological importance of each type of bacterial predator. The evolution of bacterial predation in these three groups of predators should also be studied in the future to better understand the significance of predation to biological evolution.
Our study highlights the ecological role of Bradymonadales in saline environments. Given their substantial sequence and cell frequencies in the saline environment and their storage of nutrients as polymers in cells during predation, Bradymonadales may have an alternative way of regulating global nutrient cycling. To better understand the impact of bradymonabacterial predation on regulating biogeochemical cycling, predation mutants and microcosms need to be developed in further studies.
To explore the predation of Bradymonabacteria, we used Bradymonas sediminis FA350T and Lujinxingia litoralis B210T as representative strains. All candidate prey strains were obtained from our laboratory. Cells were centrifuged, washed, and concentrated in seawater to a final OD600 of 3.0 for predator strains and 6.0 for candidate prey strains. Drops of 5.0 μl of the predator strain suspensions were deposited on the surfaces of agar plates and allowed to dry. Next, 20.0 μl drops of each different candidate prey strain suspension were placed near the predator spot. The plates were incubated at 33 °C, and images were taken after 48 h with a digital camera. To detect PHA accumulation, the granules were stained with the Nile red component of Nile blue A.
Genome sequencing and comparative genome analyses
To explore the potential metabolic capacity of bacterial predators, we sequenced 3 complete genomes and 6 draft genomes of all currently known Bradymonabacteria isolate strains. The genomic DNA of all strains was extracted with a DNA extraction kit (TaKaRa Bio) according to the manufacturer’s instructions. For strains FA350T, V1718, and YN101, complete genome sequencing was performed by Nanjing CocoBio Co., China, using the Illumina HiSeq platform accompanied with the SMRT platform to build Illumina PE and Pacbio libraries. The single-molecule sequencing data assembly was accomplished using SOAPdenovo v2.04 and Celera Assembler v8.0, and the results were rectified through BLAST searches in the BlastR database. The de novo assembly of the scaffolds was performed using Celera Assembler 8.0, which were then overlapped and trimmed using GapCloser v1.12 (SOAPdenovo-related software) . Draft genome sequencing of the other 6 strains was performed by Shanghai Personal Biotechnology Co., Ltd. (Shanghai, China) using Solexa paired-end sequencing technology . A library with an average fragment length of 400 bp was constructed, and the final genomes were assembled using SOAPdenovo version 2.04 . We also retrieved 37 predator genomes from NCBI (including 4 metagenome-assembled genomes). tRNA and gene prediction were performed using tRNAscan and prodigal, respectively. The genome-based metabolic potential of the bacterial predators was predicted by BlastKOALA (https://www.kegg.jp/blastkoala/). The average nucleic acid identities among the 9 cultured Bradymonabacteria strains were calculated using pyani (https://github.com/widdowquinn/pyani), and the percentage of conserved proteins (POCP) in each strain was calculated as described previously by Qin et al. .
Electron microscopy analyses
We selected Algoriphagus marinus am2, which is smaller than the predator Bradymonas sediminis FA350T, as prey. Bradymonas sediminis FA350T and Algoriphagus marinus am2 were cultured separately to the exponential growth phase, adjusted to the same OD value, mixed together, and cocultured on marine agar medium at 33 °C for 68 h.
For TEM analysis, mixed culture samples were supported on carbon/formvar-coated copper grids. The grids were inverted over a drop of 1% uranyl acetate. Thin sections were prepared with the predator-prey cocultures at 68 h incubation. The samples were mixed with 0.5 ml of 2% glutaraldehyde in 0.1 M sodium cacodylate buffer, centrifuged and resuspended in 1 ml of the same solution for 3 h. The cells were washed in cacodylate buffer, fixed with 1% osmium tetroxide, and encased in agar. The agar-encased cells were then fixed in 2% uranyl acetate, dehydrated through an ethanol series, and embedded in Epon resin. Thin sections were cut and stained with uranyl acetate and lead citrate. Specimens were examined with a JEM-1200EX electron microscope operated at 80 kV.
For SEM analysis, mixed culture samples were washed 3 times with PBS and fixed for 1 h in 2.5% glutaraldehyde in sodium cacodylate buffer (0.1 M, pH 7.2). To dehydrate the bacteria, the EM grids underwent a series of washes in increasing concentrations of ethanol (25, 50, 75, and 96%) and placed in a vacuum overnight. The samples were coated with gold and observed using a Nova NanoSEM 450.
Dual transcriptomic analyses
To determine the gene expression profiles of the type strain FA350T, Algoriphagus marius am2T was used as prey due to its cell morphology being different from B. sediminis FA350T. Moreover, B. sediminis FA350T predated A. marius am2T well, and the two species have a distant evolutionary relationship that was helpful for further gene mapping analysis. A pure culture of FA350T and a coculture of FA350T with the prey A. marinus am2 were cultured on marine agar medium at 33 °C for 0 h, 68 h, and 120 h, respectively. Mixed-culture cells sedimented by centrifugation and washed, and the resulting pellets stored at − 80 °C prior to RNA extraction. Each time point was collected in triplicate (n = 3) for further transcriptomic analysis. RNA was extracted using an miRNEasy mini kit (Qiagen 217004), rRNA was removed using a Ribo-Zero Magnetic kit (Bacteria) from Epicentre (MRZB12424), and cDNA library construction was performed with a TruSeq Stranded mRNA library preparation kit from Illumina (RS-122-2101) . Sequencing was carried out on a HiSeq sequencer at Novogene Co., Ltd. (Beijing, China).
Transcriptome mapping and differential expression analysis
For transcriptomic analysis of mixed culture samples (dual transcriptomic analysis), total RNA sequences were mapped to the complete genome of FA350T using the method reported by Westermann et al. . Before downstream processing, trimmomatic was used to clean the reads to remove adaptor sequences and leading and trailing bases with quality thresholds below 20, perform sliding window trimming (with parameters 4 : 15), and remove reads less than 36 bp in length. After cleaning, the remaining paired reads were mapped to the respective genomes to calculate expression values. The reference genomes used were B. sediminis FA350T (accession: CP030032.1) and A. marinus am2 (accession: MSPQ00000000.1). The output fragments per kilobase of transcript per million mapped reads (FPKM) were calculated for further analysis. Significantly upregulated and downregulated genes were defined using a false discovery rate of less than 0.001, a P value of < 0.05, and a minimum 1 log2(fold)-change of gene expression.
Phylogenetic analysis of bradymonabacterial type IV pili
An unrooted, maximum likelihood phylogeny shows relationships between the type IVa, type IVb, and type IVc pili and the archaellum (archaeal flagellum) and the T2SS and T4SS extension ATPases. The protein amino-acid sequences were aligned with mafft and used to estimate a maximum likelihood phylogeny with RAxML under the JTT substitution model with gamma-distributed rate variation. The protein amino-acid sequences of Bradymonas were annotated by RAST (Rapid Annotation using Subsystem Technology) . The other protein amino acid sequences were obtained from other research supplementary materials .
Biogeographic distribution database construction
All the 16S rRNA gene sequences analyzed in this paper were downloaded from the European Nucleotide Archive (https://www.ebi.ac.uk/ena, ENA) during or before December 2018. As a result, we collected 1552 samples from 102 projects or studies: 25 from nonsaline lake sediments (NSLS), 275 from marine sediments (MS), 499 from nonsaline soil (NSS), 45 from saline soil (SS), 216 from nonsaline water (NSW), 19 from saline lake sediments (SLS), 466 from seawater (SW), and 7 from saline lake water (SLW) (Table S5).
Microbial community composition
The raw 16S rRNA gene reads were filtered with UCHIME. Quality filtering, chimera detection, dereplication, clustering into OTUs, and assigning taxonomic information were performed using VSEARCH . The SILVA database Ref_SSU release 132 was used as a reference taxonomic database (https://www.arb-silva.de/). Alpha diversity indices (Shannon, Simpson, Good’s coverage and Ace) detailing the microbial community composition within each sample were calculated using scikit-bio (http://scikit-bio.org/) in Python, and alpha diversity indices (Chao1) were calculated using the package fossil (https://www.rdocumentation.org/packages/fossil) in R. For estimating community dissimilarities, the Bray–Curtis dissimilarity was calculated between 1551 samples (total samples: 1552) by vegan in R based on the relative abundance of order taxonomy level.
Both RAxML  and FastTree  were employed to construct the Bradymonabacteria phylogenetic tree. Given both the topology of the phylogenetic tree and its good coverage of all Bradymonabacteria lineages, we established the phylogenetic tree using 187 representative Bradymonabacteria 16S rRNA gene sequences, which were all longer than 1200 bp (at 98.5% cutoff). These sequences were aligned using mafft. The Bradymonabacteria subgroup designations were confirmed when one subgroup with > 10 representative sequences was monophyletic by two phylogenetic trees constructed by different programs using the maximum likelihood approach . The environmental type (i.e., saline and nonsaline) of each Bradymonabacteria sequence in the tree was collected from GenBank. A genome-based phylogeny of bacterial predators and 9 cultured Bradymonabacteria strains was constructed using core genes , and trees were constructed using RAxML . All phylogenetic trees were drawn using ggtree  in R.
Quantitative real-time PCR
The environmental DNA samples extracted in the previous step were used for qPCR experiments in order to detect the abundance of bacteria and Bradymonadales in each sample. The primer pair composed of 341F (5′-CCTACGGGAGGCAGCAG-3′) and 534R (5′-ATTACCGCGGCTGCTGGCA-3′) was used for quantification of bacteria . A Bradymonadales-specific primer set composed of qBRA1295F (5′-CTCAGTWCGGATYGYAGTCTG-3′) and qBRA1420R (5′-GTCACYGACTTCTGGAGCAARYG-3′), which was designed in the present study and generated an amplicon of 148 bases, was used for quantification of Bradymonadales. The specificity and coverage test of the primers were described in the Supplementary Materials (Supplementary Methods and Results, Fig. S10 and S11, Tables S8 and S9). Reactions for each sample were carried out in an ABI StepOnePlus thermal cycler under the following conditions: an initial denaturation step at 95 °C for 10 min and then 40 cycles of 15 s at 95 °C and 30 s at 60 °C. The reaction was performed in a total volume of 20 μl, composed of 10 μl 2X Universal SYBR Green Fast qPCR Mix (ABclonal), 0.4 μl of each primer (10 μM), 1 μl of sample, and 8.2 μl of MiliQ water. The Plasmid DNA Standard was constructed by introducing the 16S rDNA gene amplified from Bradymonas sediminis FA350T into the pMD18-T Vector (TaKaRa) following the manufacturer’s instructions. The plasmid was isolated and purified using a MiniBEST Plasmid Purification Kit (TaKaRa). DNA copy number was determined by the concentration and relative molecular weight of the Plasmid DNA. For each QPCR assay, the plasmid aliquot was serially diluted to produce concentrations ranging from 109 to 103 DNA copies/μl to generate calibration curves. Each sample was measured in triplicate, and negative controls (no template NTC) were included.
Availability of data and materials
The genomes of cultured bradymonabacteral isolates have been deposited in the NCBI database under GenBank accession numbers CP042467.1 (Bradymonadales strain V1718), CP042468.1 (Bradymonadales strain YN101), VOPX00000000.1 (Bradymonadales strain TMQ1), VOSL00000000.1 (Bradymonadales strain TMQ2), QRGZ00000000.1 (Bradymonadales strain TMQ3), VOSM00000000.1 (Bradymonadales strain TMQ4), CP030032.1 (Bradymonas sediminis FA350T), QHKO00000000.1 (Lujinxingia litorali B210T), and SADD00000000.1 (Lujinxingia sediminis SEH01T). The genomes of uncultured Bradymonabacteria have been deposited in the NCBI database under GenBank accession numbers PWKZ00000000.1 (Bradymonadales bin CSSed10_215), PWTN00000000.1 (Bradymonadales bin CSSed11_191), PXAJ00000000.1 (Bradymonadales bin T3Sed10_204), and PWZZ00000000.1 (Bradymonadales bin T3Sed10_190). The 16S rRNA gene data sets of Gaodao salterns have been deposited in the Sequence Read Archive under accession number SRP217756 for all the samples. The transcriptome sequences for predation of FA350T have been deposited in the NCBI database under accession numbers PRJNA559243 and PRJNA559253. All Bradymonabacteral isolates have been deposited at the Shandong Infrastructure of Marine Microbial Resources hosted by the Laboratory of Marine Microbiology at Shandong University (http://www.sdum.wh.sdu.edu.cn/search.html?itemId=14). All Bradymonabacteral isolates are available upon request. The bash, R, and python scripts for this study are available on the GitHub: https://github.com/2015qyliang/BradymonabacteriaAnalysis.
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This work was supported by the National Natural Science Foundation of China (31770002 and 41876166), National Science and Technology Fundamental Resources Investigation Program of China (2019FY100700)
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Additional file 1: Figure S1.
The General features of bacterial predators. Figure S2. General gene expression profiles of Bradymonas sediminis FA350 during mix-culturing with prey Algoriphagus marines am2. Figure S3. Gene expression profiles of Bradymonas sediminis FA350 during mix-culturing with prey Algoriphagus marines am2. Figure S4. Phylogenetic analysis of secretion system machinery reveals a distinct tad type IVb subtype of type IV pili. Figure S5. The alpha diversity in 1,552 samples. Figure S6. Principal component analysis of different samples associated with saline status. Figure S7. Global distribution of Myxococcales and Bdellovibrionales in eight different biotopes from 1,552 samples. Figure S8. The relative abundance of Bradymonadales, Myxococcales, and Bdellovibrionale. Figure S9. Cell abundance and relative abundance of gene copy number of Bradymonadales from solar saltern sediments. Figure S10. Specificity test of the Quantitative real-time PCR primers. Figure S11. Quantitative real-time PCR amplification detection and the standard curve.
Additional file 2: Table S1.
Detailed information of organisms on the phylogenetic tree
Additional file 3: Table S2.
Detailed genes information for figure 3.
Additional file 4: Table S3.
Detailed genes information for figure 4.
Additional file 5: Table S4.
Detailed key genes expression changed during predation.
Additional file 6: Table S5.
Detailed information of 16S rRNA genes amplicon samples used in this study.
Additional file 7: Table S6.
Detailed information of the representative 16S rRNA gene sequences.
Additional file 8: Table S7.
Probes designed and optimized in this study.
Additional file 9: Table S8.
Specificity and coverage of primers qBRA1295F and qBRA1420R using the SILVA database SSU r138 Ref NR.
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Mu, DS., Wang, S., Liang, QY. et al. Bradymonabacteria, a novel bacterial predator group with versatile survival strategies in saline environments. Microbiome 8, 126 (2020). https://doi.org/10.1186/s40168-020-00902-0
- Bacterial predator
- Metabolic deficiencies
- Comparative genomic analysis
- Biogeographic analysis