Open Access

Correction to: Optimization and validation of sample preparation for metagenomic sequencing of viruses in clinical samples

  • Dagmara W. Lewandowska1,
  • Osvaldo Zagordi1,
  • Fabienne-Desirée Geissberger1,
  • Verena Kufner1,
  • Stefan Schmutz1,
  • Jürg Böni1,
  • Karin J. Metzner1, 2,
  • Alexandra Trkola1 and
  • Michael Huber1Email author
Microbiome20175:137

https://doi.org/10.1186/s40168-017-0351-x

Received: 25 September 2017

Accepted: 25 September 2017

Published: 12 October 2017

The original article was published in Microbiome 2017 5:94

Correction

Following publication of the original article [1], the authors were alerted by a colleague of a column duplication in Table 1. Since the summary row was correct, though, the interpretation and the conclusion of the article were not affected
Table 1:

Number of reads reported from sequencing a multiplexed viral pathogen reagent

 

Replicate 1

Replicate 2

Virus species (most frequent strain)

all 3'505'318 reads

2'000'000 reads

150'000 reads

all 2'676'604 reads

2'000'000 reads

150'000 reads

25 target viruses

      

 Human astrovirus (Human astrovirus 1)

27'622

15’783.2

1’184.1

10'781

8’040.6

596.2

 Enterovirus B (Human coxsackievirus B4)

27'252

15’539.0

1’161.3

30'409

22’707.3

1’704.4

 Human herpesvirus 1

67

39.0

3.5

106

81.4

5.5

 Human herpesvirus 2

2

1.3

0.4

9

7.0

0.4

 Human herpesvirus 3 (Human herpesvirus 3 strain Dumas)

34

17.4

1.4

105

84.2

6.4

 Human herpesvirus 4

166

98.1

8.2

149

104.7

8.7

 Human herpesvirus 5

8'327

4’743.1

352.1

9'398

7’028.5

524.4

 Human mastadenovirus C (Human adenovirus 2)

23

11.5

0.8

404

299.1

19.6

 Human mastadenovirus F (Human adenovirus 41)

2

2.0

0.0

19

13.9

1.7

 Human metapneumovirus

0

0.0

0.0

0

0.0

0.0

 Human parainfluenza virus 1

21'387

12’170.8

921.2

12'897

9’601.2

718.6

 Human parainfluenza virus 2

2'879

1651.5

119.6

44

33.1

1.6

 Human parainfluenza virus 3

0

0.0

0.0

0

0.0

0.0

 Human parainfluenza virus 4 (Human parainfluenzavirus 4b)

21'858

12’478.6

924.3

13'456

10’089.8

760.2

 Human respiratory syncytial virus

0

0.0

0.0

298

229.4

15.5

 Human Rhinovirus A (Human rhinovirus A39)

0

0.0

0.0

2'983

2’238.4

158.6

 Influenzavirus A H1N1

0

0.0

0.0

0

0.0

0.0

 Parechovirus A (Human parechovirus 3)

1'492'756

851’560.6

63862.7

756'598

565’228.5

42’434.5

 Rotavirus A

6

2.8

0.3

12

8.5

0.8

 Sapporovirus (Sapovirus Hu/GI.2/BR-DF01/BRA/2009 and Hu/G1/BE-HPI01/DE/2012)

1'019

575.5

45.2

82

62.8

5.1

 Human coronavirus 229E

0

0.0

0.0

0

0.0

0.0

 Norovirus GI

0

0.0

0.0

0

0.0

0.0

 Norovirus GII

0

0.0

0.0

0

0.0

0.0

 Influenza virus B

0

0.0

0.0

0

0.0

0.0

 Influenza virus A H3N2

0

0.0

0.0

0

0.0

0.0

Non-target viruses

      

 Bovine viral diarrhea virus 1

1'446

820.6

63.6

2'526

1’891.8

140.3

 Bovine viral diarrhea virus 2

1

0.4

0.4

0

0.0

0.0

 Primate bocaparvovirus 1

0

0.0

0.0

0

0.0

0.0

 Primate bocaparvovirus 2

208

118.2

7.5

117

91.6

6.8

 Human enterovirus (Enterovirus CA55-1988)

3'199

1’834.7

140.6

495

380.6

28.7

 Aichi virus

0

0.0

0.0

0

0.0

0.0

 Ungulate bocaparvovirus 1

0

0.0

0.0

0

0.0

0.0

 Porcine/other circovirus

0

0.0

0.0

0

0.0

0.0

Total virus reads

1'608'254

917'448.3

68'797.2

840'888

628'222.4

47'138.0

Number of detected target viruses

15

14.4

11.7

17

17.0

15.7

For subsamples of reads the average number of virus reads and detected target viruses in 10 random samplings is shown

.
The authors have provided the correct table, and would like to acknowledge the assistance of their colleague in the following words: “We thank Maia Segura-Wang for helpful corrections.”

Declarations

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Authors’ Affiliations

(1)
Institute of Medical Virology, University of Zurich
(2)
Department of Infectious Diseases and Hospital Epidemiology, University Hospital Zurich

Reference

  1. Lewandowska DW, Zagordi O, Geissberger F-D, Kufner V, Schmutz S, Böni J, Metzner KJ, Trkola A, Huber M. Optimization and validation of sample preparation for metagenomic sequencing of viruses in clinical samples. Microbiome. 2017;5:94. doi:10.1186/s40168-017-0317-z.View ArticlePubMedPubMed CentralGoogle Scholar

Copyright

© The Author(s). 2017

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