Table 1: Number of reads reported from sequencing a multiplexed viral pathogen reagent
Replicate 1 | Replicate 2 | |||||
|---|---|---|---|---|---|---|
Virus species (most frequent strain) | all 3'505'318 reads | 2'000'000 reads | 150'000 reads | all 2'676'604 reads | 2'000'000 reads | 150'000 reads |
25 target viruses | ||||||
Human astrovirus (Human astrovirus 1) | 27'622 | 15’783.2 | 1’184.1 | 10'781 | 8’040.6 | 596.2 |
Enterovirus B (Human coxsackievirus B4) | 27'252 | 15’539.0 | 1’161.3 | 30'409 | 22’707.3 | 1’704.4 |
Human herpesvirus 1 | 67 | 39.0 | 3.5 | 106 | 81.4 | 5.5 |
Human herpesvirus 2 | 2 | 1.3 | 0.4 | 9 | 7.0 | 0.4 |
Human herpesvirus 3 (Human herpesvirus 3 strain Dumas) | 34 | 17.4 | 1.4 | 105 | 84.2 | 6.4 |
Human herpesvirus 4 | 166 | 98.1 | 8.2 | 149 | 104.7 | 8.7 |
Human herpesvirus 5 | 8'327 | 4’743.1 | 352.1 | 9'398 | 7’028.5 | 524.4 |
Human mastadenovirus C (Human adenovirus 2) | 23 | 11.5 | 0.8 | 404 | 299.1 | 19.6 |
Human mastadenovirus F (Human adenovirus 41) | 2 | 2.0 | 0.0 | 19 | 13.9 | 1.7 |
Human metapneumovirus | 0 | 0.0 | 0.0 | 0 | 0.0 | 0.0 |
Human parainfluenza virus 1 | 21'387 | 12’170.8 | 921.2 | 12'897 | 9’601.2 | 718.6 |
Human parainfluenza virus 2 | 2'879 | 1651.5 | 119.6 | 44 | 33.1 | 1.6 |
Human parainfluenza virus 3 | 0 | 0.0 | 0.0 | 0 | 0.0 | 0.0 |
Human parainfluenza virus 4 (Human parainfluenzavirus 4b) | 21'858 | 12’478.6 | 924.3 | 13'456 | 10’089.8 | 760.2 |
Human respiratory syncytial virus | 0 | 0.0 | 0.0 | 298 | 229.4 | 15.5 |
Human Rhinovirus A (Human rhinovirus A39) | 0 | 0.0 | 0.0 | 2'983 | 2’238.4 | 158.6 |
Influenzavirus A H1N1 | 0 | 0.0 | 0.0 | 0 | 0.0 | 0.0 |
Parechovirus A (Human parechovirus 3) | 1'492'756 | 851’560.6 | 63862.7 | 756'598 | 565’228.5 | 42’434.5 |
Rotavirus A | 6 | 2.8 | 0.3 | 12 | 8.5 | 0.8 |
Sapporovirus (Sapovirus Hu/GI.2/BR-DF01/BRA/2009 and Hu/G1/BE-HPI01/DE/2012) | 1'019 | 575.5 | 45.2 | 82 | 62.8 | 5.1 |
Human coronavirus 229E | 0 | 0.0 | 0.0 | 0 | 0.0 | 0.0 |
Norovirus GI | 0 | 0.0 | 0.0 | 0 | 0.0 | 0.0 |
Norovirus GII | 0 | 0.0 | 0.0 | 0 | 0.0 | 0.0 |
Influenza virus B | 0 | 0.0 | 0.0 | 0 | 0.0 | 0.0 |
Influenza virus A H3N2 | 0 | 0.0 | 0.0 | 0 | 0.0 | 0.0 |
Non-target viruses | ||||||
Bovine viral diarrhea virus 1 | 1'446 | 820.6 | 63.6 | 2'526 | 1’891.8 | 140.3 |
Bovine viral diarrhea virus 2 | 1 | 0.4 | 0.4 | 0 | 0.0 | 0.0 |
Primate bocaparvovirus 1 | 0 | 0.0 | 0.0 | 0 | 0.0 | 0.0 |
Primate bocaparvovirus 2 | 208 | 118.2 | 7.5 | 117 | 91.6 | 6.8 |
Human enterovirus (Enterovirus CA55-1988) | 3'199 | 1’834.7 | 140.6 | 495 | 380.6 | 28.7 |
Aichi virus | 0 | 0.0 | 0.0 | 0 | 0.0 | 0.0 |
Ungulate bocaparvovirus 1 | 0 | 0.0 | 0.0 | 0 | 0.0 | 0.0 |
Porcine/other circovirus | 0 | 0.0 | 0.0 | 0 | 0.0 | 0.0 |
Total virus reads | 1'608'254 | 917'448.3 | 68'797.2 | 840'888 | 628'222.4 | 47'138.0 |
Number of detected target viruses | 15 | 14.4 | 11.7 | 17 | 17.0 | 15.7 |