Chemical and pathogen-induced inflammation disrupt the murine intestinal microbiome
© The Author(s). 2017
Received: 21 December 2016
Accepted: 11 April 2017
Published: 27 April 2017
Salmonella is one of the most significant food-borne pathogens to affect humans and agriculture. While it is well documented that Salmonella infection triggers host inflammation, the impacts on the gut environment are largely unknown. A CBA/J mouse model was used to evaluate intestinal responses to Salmonella-induced inflammation. In parallel, we evaluated chemically induced inflammation by dextran sodium sulfate (DSS) and a non-inflammation control. We profiled gut microbial diversity by sequencing 16S ribosomal ribonucleic acid (rRNA) genes from fecal and cecal samples. These data were correlated to the inflammation marker lipocalin-2 and short-chain fatty acid concentrations.
We demonstrated that inflammation, chemically or biologically induced, restructures the chemical and microbial environment of the gut over a 16-day period. We observed that the ten mice within the Salmonella treatment group had a variable Salmonella relative abundance, with three high responding mice dominated by >46% Salmonella at later time points and the remaining seven mice denoted as low responders. These low- and high-responding Salmonella groups, along with the chemical DSS treatment, established an inflammation gradient with chemical and low levels of Salmonella having at least 3 log-fold lower lipocalin-2 concentration than the high-responding Salmonella mice. Total short-chain fatty acid and individual butyrate concentrations each negatively correlated with inflammation levels. Microbial communities were also structured along this inflammation gradient. Low levels of inflammation, regardless of chemical or biological induction, enriched for Akkermansia spp. in the Verrucomicrobiaceae and members of the Bacteroidetes family S24-7. Relative to the control or low inflammation groups, high levels of Salmonella drastically decreased the overall microbial diversity, specifically driven by the reduction of Alistipes and Lachnospiraceae in the Bacteroidetes and Firmicutes phyla, respectively. Conversely, members of the Enterobacteriaceae and Lactobacillus were positively correlated to high levels of Salmonella-induced inflammation.
Our results show that enteropathogenic infection and intestinal inflammation are interrelated factors modulating gut homeostasis. These findings may prove informative with regard to prophylactic or therapeutic strategies to prevent disruption of microbial communities, or promote their restoration.
KeywordsSalmonella Short-chain fatty acids Inflammation Beta diversity LEfSe CBA/J Lipocalin-2
The bacterial species Salmonella enterica includes over 2500 serovars . One of the most common causes of human gastroenteritis is serovar Typhimurium . This serovar has long served as a model organism for studies of pathogenesis in murine models. Upon ingestion, Salmonella injects effector proteins into the host intestinal epithelial cells . These effectors trigger the uptake of Salmonella into the host cells and initiate inflammation that disrupts the microbiota. This disruption presumably reduces competition for nutrients, and it also causes an oxidative burst that leads to the accumulation of tetrathionate, nitrate, and oxygen, all of which are used as respiratory electron acceptors by Salmonella [4–10]. This respiratory metabolism confers a growth advantage to Salmonella over the fermentative commensal bacteria, allowing this pathogen to rapidly proliferate within the intestinal microbial community.
One major issue with mouse models is that mice are highly resistant to Salmonella-induced inflammation, which is thought to be due to the mouse gut microbiota rather than the host itself. Either the use of germ-free mice or the disruption of the microbiota with antibiotics allows Salmonella to induce inflammation in most murine models [11–14]. Unfortunately, the germ-free or antibiotic-treated models are not conducive with regard to understanding how Salmonella alters the response of the commensal microbial community. Alternatively, it was recently discovered that the CBA/J murine model allows persistent colonization of the gastrointestinal tract by Salmonella, which eventually leads to inflammation approximately ten days post-infection [15, 16]. In this report, we use CBA/J mice to study the disruption of the healthy microbiota by Salmonella. Additionally, to begin to separate the microbiome response to inflammation alone rather than inflammation and the pathogen, we compare the pathogen-induced inflammation to chemically induced inflammation caused by dextran sodium sulfate (DSS). DSS increases the permeability of the mucosal barrier, allowing commensal microbiota to contact the epithelium and trigger an inflammatory response [17, 18].
Salmonella-mediated disruption of the commensal microbiota has been previously studied; however, the microbiota was characterized at a broad taxonomic level or using an antibiotic-treated mouse model [5, 7, 19, 20]. Here, we characterize the otherwise undisturbed fecal and cecal communities before and after disruption by Salmonella or DSS and focus on operational taxonomic unit (OTU) level responses. We measure changes in microbial community diversity and membership, as well as changes in the chemical environment and compare these responses to non-inflamed control mice. Results from this study provide an in-depth insight into Salmonella impacts on the gut environment. These findings may reveal new therapeutic strategies for prebiotics or probiotics for maintaining or restoring the microbiota in response to Salmonella perturbation [21–28]. Furthermore, examining responses of the commensal microbiota and chemical environment to inflammation has broader ramifications to other gastrointestinal diseases, including ulcerative colitis, Crohn’s disease, and colon cancer [29–31].
Experimental design and 16S rRNA gene sequencing
Inflammation type (chemical or biological) structures gut microbial communities
Within the Salmonella treatment group, we saw a variable response, as two distinct clusters were observed on the NMDS, a finding confirmed by beta-dispersion analyses. The Salmonella treatment had the greatest within treatment variability compared to the other treatment groups (mean distance to centroid: Control = 0.306, DSS = 0.316, Salmonella = 0.470). Despite using identical inoculation concentrations and procedures, Salmonella relative abundance ranged from <1 to 71% in the late fecal and cecal samples. At the end of the experiment, three of the mice had much higher Salmonella relative abundance than the other seven mice, with this high-responder group defined as having a minimum Salmonella relative abundance of 49 and 46% in the late time point fecal and cecal samples, respectively. This differential Salmonella response was also visually verified upon autopsy, as ceca in the high responders were pus-filled, unlike the other Salmonella-treated mice (Additional file 3: Figure S1). Alternatively, the low responders had a maximum of 7 and 0.5% in the late time point fecal and cecal samples, respectively. This range in Salmonella relative abundance was represented in the NMDS clustering, with high responders (red) clustering distinctly from low responders (orange) and control (blue) (multi-response permutation procedures (mrpp), p value <0.05). While the latter two groups were statistically different, low responders were more similar to the control than high responders. Interestingly, this clustering was maintained when the Salmonella OTU was removed from the analyses (Additional file 4: Figure S2; see the “Methods” section), demonstrating that the differences observed between treatments were not attributed solely to pathogen increase, but rather the overall impacts of the pathogen on the surrounding microbial community.
Our data clearly show that mice given the same Salmonella dose and treatment have variable susceptibility to Salmonella colonization. To confirm that we did not underestimate Salmonella relative abundance in the low-responder group due to our sampling schedule, we subsequently sequenced all fecal time points between the pre-treatment and late samples (Additional file 5: Figure S3). Salmonella relative abundance in the low-responder group was not uniform over time. Across all time points in the low responders, the maximum Salmonella relative abundance for each mouse ranged from 0.3 to 16%. This analysis showed that Salmonella relative abundance was higher at earlier time points, compared to the final time points we reported initially, but more importantly confirmed the low-responder group never reached our designated high-responder Salmonella relative abundance (>46%). Our findings confirm that we did not miss the sampling window of elevated Salmonella response in the low-responder mice. For subsequent analyses, we divided the Salmonella infected samples into “low-responder” and “high-responder” treatment groups.
Inflammation and metabolites are correlated with treatment groups
Microbially produced short-chain fatty acids (SCFA) maintain the gut barrier, participate in host signaling, and contribute to host energy , so we were interested in the impacts of inflammation on cecal SCFA concentrations. The cumulative concentrations of acetate, butyrate, and propionate (here reported as total SCFA) in the ceca were not significantly different between the control, DSS, or low-responder groups, but were significantly decreased in the high-responder group (Fig. 4a, Additional file 6). Individual SCFA had a treatment specific response. Acetate, butyrate, and propionate were all below detection in the high-responder group, while relative to the control, the DSS and low responders had significantly decreased butyrate and increased propionate concentrations, while acetate increased significantly only in the DSS group. Across all samples, the amount of inflammation negatively correlated to the total concentration of short-chain fatty acids and to butyrate concentrations. At a more global level, changes in the chemical environment (e.g., inflammation and SCFA concentrations) corresponded to changes in cecal microbial community structure (Fig. 4b) (envfit, p < 0.001).
Identifying key microbial determinants for each treatment group
To be consistent with prior reports examining the impacts of Salmonella colonization on the gut microbiota [5–7, 19, 20, 35], we examined our data to see if groupings observed on the NMDS (Fig. 2) were consistent with changes in membership at the class level. Control fecal microbial communities at day 14 were dominated by Firmicutes and Bacteroidetes phyla, especially within the Clostridia (62 ± 9%) and Bacteroidia (34 ± 8%) classes (Additional file 8: Figure S4). Despite differences in SCFA and inflammation levels, Clostridia and Bacteroidia relative abundance did not change significantly in the low-responders or DSS groups relative to the control. Notably, the relative contribution of these two classes was significantly decreased when Salmonella exceeded 46% relative abundance (e.g., high-responder group only), but was not correlated to Salmonella relative abundance across the experiment. Relative to the other treatments, microbial communities in the high-responder group were enriched in the Gammaproteobacteria (57%, driven largely by Salmonella) and the Bacilli classes (Additional file 8: Figure S4).
Our findings showed that differences in inflammation type (chemical or biological) and inflammation amount (DSS and low responder compared to high responder) corresponded to altered microbial membership. In the chemical but not the biological inflamed guts, LEfSe identified six genera unique to the DSS communities including two genera within the Lachnospiraceae (Coprococcus and an uncultured genus) and two uncultured genera within the Ruminococcaceae. At day 15, Coprococcus had a significantly higher mean relative abundance in the DSS treatment group (2.2 ± 0.4%), compared to all other groups (<0.05%). These findings suggest taxa are finely tuned to tolerate the low levels of inflammation induced by the chemical DSS treatment and thus could be responsible for increased acetate production observed only in this treatment relative to the control.
The low-responder group had higher levels of inflammation relative to the control and DSS groups (Fig. 4). In the low responder communities, LEfSe identified eight genera including members of Akkermansia, Roseburia, and a formerly uncultivated Bacteroidetes family S24-7 (Candidatus Homeothermaceae or Muribaculaceae) [38, 39]. Interestingly, several of these taxa were also enriched by chemical inflammation (DSS), but were not detected in the control or high responder groups. For instance, Akkermansia mean relative abundance was elevated in DSS (3%) and low responders (9%) but not detected in high responders or control (<0.01%). The same response was observed for S24-7 (Fig. 5). This result suggests that these taxa respond positively to low levels of inflammation but are decreased when Salmonella relative abundance or inflammation is high. These taxa that co-occur across both treatments may also be responsible for increased propionate observed in both the DSS and low-responder groups,
The Salmonella high responders group had the most elevated levels of inflammation (Figs. 4a and 5a). Besides Salmonella (LDA = 5.3), LEfSe identified five significant Enterobacteriaceae genera within the Gammaproteobacteria (e.g., Enterobacter, Citrobacter, Enterococcus, Escherichia/Shigella) that distinguished the high-responder microbial communities from all other groups (Fig. 5). LEfSe also identified Lactobacillus as discriminant taxa in the high responders, and this genus is the primary driver of the Bacilli class response enriched in all Salmonella-treated mice, accounting for on average 9 and 26% in the low- and high-responder treatment groups, respectively. This response suggests that Lactobacillus may co-enrich with the presence of Salmonella and may not be responding to the high levels of inflammation.
Principal findings of the study
Here, we investigated the impacts of Salmonella expansion in the mouse gastrointestinal tract on the commensal microbial community and the chemical environment. To our knowledge, this is the first study to directly compare and contrast DSS-induced chemical inflammation with Salmonella-induced inflammation. Our principle findings are the following: (1) the extent of Salmonella colonization in the mouse gastrointestinal tract is variable; (2) gut microbial community membership is congruent with Salmonella relative abundance and inflammation level; (3) commensal Alistipes and Lachnospiraceae taxa decrease along an inflammation gradient; (4) low levels of inflammation (regardless of source) increase the relative abundance of Akkermansia and S24-7; and (5) Lactobacilli and members of the Enterobacteriaceae are co-enriched with Salmonella.
Mice have differential Salmonella susceptibility
Here, we report infected mice have a large range in Salmonella colonization relative abundance (<1 to 71%), with three of the ten mice classified as high (>46%) responders. This variation was shared between cecal and fecal samples, demonstrating it was not an artifact of gastrointestinal tract sampling location. We did verify that timing had a negligible effect on Salmonella relative abundance, as some of the low responders had increased abundance at earlier time points (0.25–16%) relative to the last time points we sampled (0.25–7%). Importantly, however, none of the low-responder mice ever had sufficient Salmonella relative abundance to be considered a member of the high-responder group; thus, the groupings likely reflect the unique chemical and biological environments caused by different amounts pathogen induced inflammation.
While we recognize our high responder sample size is limited (n = 3), similar to our findings, others have hinted at this variation (and also recovered high responders) in Salmonella relative abundance in infected mice. Our findings expand upon prior studies in several ways [5–7, 19, 20, 35]. First, most prior studies used a “streptomycin mouse model,” where antibiotics are given prior to Salmonella inoculation, thereby confounding whether the observed Salmonella relative abundance variation was due to an altered initial microbial community or Salmonella colonization effectiveness. In contrast, the CBA/J mouse model does not require antibiotic treatment prior to inoculation; thus, our Salmonella treated mice had highly similar initial microbial community membership and structure (Fig. 2; Bray-Curtis similarity >93% for pre-treatment communities), despite clear differences in the terminal communities.
Second, the variability in Salmonella relative abundance may not have been emphasized previously due to differences in data reporting, as studies often report the average Salmonella relative abundance in the terminal sample for the entire treatment. For instance, reporting our fecal data in that fashion would result in an average Salmonella relative abundance of 19%, absolutely consistent with prior reports of ~25%, but obscuring the true variation. Analysis of published data sets with the same mouse model (n = 5), but using the high-responder criterion established here (>46%), revealed a high responder rate of 33% , which was nearly identical to the 30% observed here (n = 10). Here, we are the first to quantify Salmonella infection efficiency and report differential chemical and microbial responses caused by variability. Future quantification of pathogen relative abundance can better inform investigations on the host and microbiome mechanisms underlying Salmonella resistance, a feature also commonly reported in humans [40–42].
Inflammation gradient correlates with SCFA and microbial community profile
Hosts rely on their gut microbiota to produce SCFA for energy, with acetate, propionate, and butyrate being the most abundant. Furthermore, SCFA are often implicated as regulators for intestinal inflammation. Many human and animal studies show decreased SCFA concentration with intestinal inflammation including instances of enteropathogenic infections, colitis, and irritable bowel syndrome [43–45]. Here, we also show the SCFA types (acetate, butyrate, propionate), and overall total concentrations are altered by changes in inflammation amount and source (biological and chemical). These changes in SCFA corresponded to differences in microbial membership across treatment groups.
The chemical environment of the gut was reflected in the microbial community, as the inflammation gradient and SCFA concentrations were significantly related to microbial community sample clustering. The most obvious changes in SCFA occurred in the highly inflamed Salmonella gut, where SCFA were significantly depleted relative to the non-inflamed control. Consistent with prior reports in germ-free mice , decreased SCFA levels may feedback to further exacerbate inflammation, contributing to the 3 log-fold higher lipocalin-2 levels detected in our high responder Salmonella samples.
We consider several possibilities for the drastically decreased SCFA in the high-responder group. First, it is possible that SCFA are not produced due to significant remodeling of the microbial community (decreased richness and membership of commensal taxa). Baumler and colleagues first reported that the relative abundance of commensal taxa (especially members of the class Clostridia) and butyrate decreased with Salmonella infection , and this decreasing butyrate , as well as reactive oxygen species from inflammation, provided alternative electron acceptors like oxygen, nitrate, and tetrathionate that allow for Salmonella expansion in the gut . Here, we extend the findings to include decreased acetate and propionate co-occurring with Salmonella expansion and Clostridia reduction in the high-responder group. Furthermore, our low responder and DSS groups shed new light on the links between Salmonella and butyrate. For example, in the DSS and low-responder groups, butyrate decreased significantly from the control, while there was no significant change in Clostridia class level relative abundance, showing that this response is due to inflammation (DSS or Salmonella induced) and not just the pathogen presence. A second explanation for the decreased SCFA in the high responders is that these mice may have consumed less food, yielding less SCFA. A third explanation for below detectable SCFA only in the high responder group is that SCFA may be rapidly consumed by Salmonella and other respiratory Enterobacteriaceae taxa stimulated by presence of alternative electron acceptors produced during high inflammation levels [9, 10, 43]. Our findings demonstrate the need for time series metatranscriptomic studies that account for butyrate production and consumption activities in light of inflammation and pathogen expansion.
Microorganisms depleted by inflammation
Given the class Clostridia contains over 12 families with considerable OTU richness (457,466 OTUs), and broad metabolic diversity [46–48], we examine the relationship between Salmonella and Clostridia at more resolved taxonomic levels (genus, OTU). For instance, within the Clostridia class, different Lachnospiraceae OTUs were enriched in each treatment (high, low, and DSS), with one OTU even increasing in response to Salmonella (New.ReferenceOTU56). Consistent with the differential response of certain members of the Lachnospiraceae across treatments, the genomic potential of the known isolates is also diverse. Of the 69 isolate Lachnospiraceae genomes, 82 and 97% encode the capacity for butyrate and acetate production, respectively. Together, these findings demonstrate that moving forward, OTU level changes should be considered, as members of the class Clostridia are present in all treatments, but strains are niche differentiated enabling unique biological and chemical treatment responses.
In addition to response of specific Clostridial members, our results show that a single dominant Alistipes OTU, a member of the Bacteroidia, decreased significantly in all defined groups relative to the control at day 15 (Fig. 5). This OTU is a defining feature of the non-inflamed microbial community, and given the inclusion of a DSS chemical inflammation treatment, we can clearly show that this response is not necessarily to Salmonella but rather inflammation. Alistipes depletion in inflamed gut environments has been demonstrated in humans with liver disease and in murine models for colitis [49–51]. We show a correlation between decreasing Alistipes relative abundance and decreasing butyrate concentrations, findings that may be attributed to end products of Alistipes metabolism. We mined publically available Alistipes isolate genomes and found that 14 out 14 genomes contain the capacity for butyrate production via butyrate kinase (see the “Methods” section). It is also possible that Alistipes produce butyrate from amino acids, as metagenomic studies have shown that members of the Alistipes have the capacity to ferment lysine to produce butyrate [52, 53]. We also mined publically available Alistipes genomes for this capacity and found that 14% of Alistipes genomes have the entire pathway to produce butyrate from lysine (see the “Methods” section for pathway). Furthermore, isolate studies show succinate as a significant end product of Alistipes metabolism which may stimulate butyrate production by other commensal microorganisms in the gut through the succinate pathway [54, 55]. While our Alistipes OTU is <95% similar to 16S sequences in isolate genomes, our findings taken together with Alistipes genomic evidence and Alistipes isolate studies suggest that future Salmonella work should be expanded beyond Clostridia to examine the contribution of Alistipes to maintaining host homeostasis.
Known mucin-degrading microorganisms are enriched by low-level inflammation
Our experimental design allowed us to investigate the response to inflammation amount regardless of the causative agent (e.g., chemical, pathogen), with low inflammation levels represented by the DSS and low-responder groups. It has been documented that inflammation upregulates a dose-dependent host response, such that lower levels stimulate mucin production while high levels inhibit mucin production [56, 57]. Mucin is the major protective component in the gastrointestinal epithelium, providing a barrier between human epithelial cells and invading pathogens. Akkermansia, a member of the Verrucomicrobia, is known to degrade mucin as its sole carbon and nitrogen source . Consistent with its role as a mucin degrader, Akkermansia is increased from controls only during treatments with low levels of inflammation, when mucin may be produced. In addition, recent genomic evidence also suggested members of the family S24-7 have similar mucin degradation capacity to Akkermansia, perhaps explaining the co-occurrence pattern of these two taxa in our low inflammation treatments . Our findings suggest it is the amount (low not high) of inflammation not the source that dictates Akkermansia and S24-7 relative abundance, findings which may explain the lack of congruence between inflammation and Akkermansia in the literature today [58–62].
Another interesting finding from our study is that propionate concentrations increased in both the low-inflammation treatments. Given the enrichment of Akkermansia and S24-7 in both these low-inflammation treatments, we mined publically available genomes for propionate production. Of the three known pathways for propionate production , the pathway that proceeds through succinate via methylmalonyl-CoA decarboxylase (E.C. 22.214.171.124) is the most prevalent in these genomes. Both Akkermansia genomes and the recently published genomes within the family S24-7 encode the capacity for propionate production . This increased propionate production may be a positive feedback on inflammation, as recent reports have suggested propionate stabilizes inflammation in the gut [64, 65]. Our findings provide insight into possible microbial probiotics that may enhance propionate stabilization through addition or stimulation of key taxa found here, including Akkermansia and members of S24-7.
Bacterial taxa that benefit from Salmonella-triggered inflammation
We show that specific members of the Proteobacteria are exclusively enriched when Salmonella relative abundances exceed 46% (high responders, n = 3). Co-enrichment of members of the family Enterobacteriaceae with Salmonella infection has also been reported in several other mouse models [5, 7, 10, 20, 66]. Additionally, a study conducted on feces from human patients with Salmonella gastroenteritis reported an increase in Enterobacteriaceae including Citrobacter, which was one of the most abundant members in our study . These findings suggest that high amounts of Salmonella and concurrent high inflammation levels induce specific microbial community changes different from low inflammation levels, which favors the expansion of closely related organisms [7, 66].
Our findings also show enrichment of Lactobacillus within the Salmonella (low and high) treatment. Lactobacillus is generally used as a probiotic for enteric infections, as some of these organisms secrete compounds that inhibit Salmonella virulence factors and motility . To further investigate if Lactobacillus increased with Salmonella in other studies, we mined 16S rRNA reads that were publically available from studies with the same mouse model as ours . Interestingly, we found enrichment of two Lactobacillus OTUs from the colon of Salmonella-infected mice. Similarly, in humans with enteric infections including Salmonella, Lactobacillus increased in infected patients [34, 35]. While the Lactobacillus OTUs are not shared across these studies, this finding suggests the relationship between Salmonella and Lactobacillus may be significant. One possibility is that the Lactobacillus members enriched during Salmonella infection are functionally distinct from the strains used as probiotics. Alternatively, the enrichment of Lactobacillus may be what eventually helps to eliminate Salmonella, consistent with the efficacy of probiotics [68, 69].
Enhancing our understanding of how intestinal microbial communities change in response to inflammation is critical to managing a multitude of diseases including enteric infection, Crohn’s disease, irritable bowel syndrome, and colon cancer. Here, we report microbial and chemical changes in the host gut environment in response to DSS- and Salmonella-induced inflammation, in order to distinguish between changes caused by enteropathogenic takeover and those caused by intestinal inflammation. While several studies have also shown that Salmonella-induced inflammation causes an enrichment of Enterobacteriaceae and depletion of Clostridia [5, 7, 9, 35], these identifications were at a broad taxonomic level. The effects of enteropathogenic expansion and the consequences of host inflammation on the intestinal microbiota are only beginning to be elucidated. Further metagenomic studies with strain-resolved information and paired transcript data are required to understand how the key taxa presented here are enhanced or diminished in response to the unique chemical environment created by increased Salmonella biomass.
Strains and media
S. enterica serovar Typhimurium strain 14028 (S. Typhiumurium 14028) was grown in Luria-Bertani (LB) broth in a roller drum at 37 °C overnight. For inoculation, the overnight culture was washed and resuspended in water.
Animals and experimental design
Female, age-matched (6 to 10 weeks old) CBA/J mice were purchased from Taconic Farms, Inc. Animals were housed in groups of five by treatment (Control = 1 cage of 5, DSS = 1 cage of 5, and Salmonella = 2 cages of 5) and were fed ad libitum Harlan mouse chow (mean 16% protein, 5% fat, 3.5% crude fiber).
Mice in the control group (n = 5) did not receive any treatment throughout the experiment. Concurrent with controls, experimental inflammation treatments, DSS (dextran sulfate sodium) (abiotic inflammation) and Salmonella (biotic inflammation), were run for 16 days. Mice in the DSS group (n = 5) received 4% DSS in drinking water the entire duration of the experiment. We selected DSS as a control to our pathogen-induced inflammation, as it is commonly used to initiate an inflammatory response  and has been shown that DSS is not a substrate that supports growth of intestinal microflora . Mice in the Salmonella group (n = 10) were orally inoculated with 109 CFU of a washed overnight culture of S. typhimurium 14028 on day 0 with no subsequent treatment. This animal experiment was performed using protocols approved by The Ohio State University Institutional Animal Care and Use Committee (IACUC; OSU 2009A0035).
Fecal samples were collected from all mice for three consecutive days prior to treatment on day 0 and prior to sacrifice on day 16. Fecal pellets were collected from each mouse on autoclaved aluminum foil. Fecal pellets were immediately transferred to pre-labeled microcentrifuge tubes, flash frozen in liquid nitrogen, and stored at -80 °C until further processing. Cecal samples were harvested from all mice on day 16 post-treatment, flash frozen in liquid nitrogen, and then stored at −80 °C until further processing.
DNA extraction and sequencing
Total nucleic acids were extracted using the PowerSoil DNA Isolation kit (MoBio), eluted in 100 μl of elution buffer provided, and stored at −20 °C until sequencing. DNA was submitted for sequencing at Argonne National Lab at the Next Generation Sequencing facility using Illumina MiSeq with 2 × 251 bp paired end reads following established HMP protocols . Briefly, universal primers 515F and 806R were used for PCR amplification of the V4 hypervariable region of 16S rRNA gene using 35 cycles. The 515F primer contained a unique sequence tag to barcode each sample. Both primers contained sequencer adapter regions.
16S rRNA data processing
Data processing was performed using QIIME 1.9.0, with specific processing steps as follows [72, 73]. Briefly, raw fastq data were demultiplexed and quality filtered to a Phred score of 20. OTUs were chosen in a two-step process. First, sequences were clustered into OTUs using UCLUST followed by de novo OTU picking. OTUs were checked for chimeras using RDP gold database and assigned taxonomy using the 97_SILVA_111 rep set . Sequences were used for comparison of the relative abundance of OTUs in at least five samples. A total of 6078 OTUs were found when using at least one sample and did not alter beta diversity of samples, as the NMDS had all of the same attributes (stress, ANOSIM, mrpp, betadispersion). Raw reads were deposited on NCBI under bioproject PRJNA348350 (pending). The final OTU table and a fasta file for above methods are included (Additional files 1 and 2). Raw reads from Chavez et al. were downloaded from NCBI and processed as above.
We inferred metabolic capacity of key taxa identified in our 16S rRNA analysis by using publically available genomes. Specified genes (e.g., methylmalonyl-CoA decarboxylase, butyrate kinase) were queried to each genome using BLASTp . Genomes were accessed via NCBI and JGI-IMG (analysis performed with data from December 2016) [75, 76]. Alistipes genomes were mined for the lysine pathway: lysine-2,3-aminomutase (EC 126.96.36.199), lysine-5,6-amino mutase (alpha and beta, EC 188.8.131.52), 3,5-diaminohexanoate dehydrogenase (EC 184.108.40.206), 3-keto-5-aminohexanoate cleavage enzyme (EC 220.127.116.11), 3-aminobutyryl-CoA ammonia-lyase (EC 18.104.22.168), butyryl-CoA dehydrogenase (EC 22.214.171.124), and butyryl-CoA:acetoacetate CoA-transferase beta subunit (alpha and beta, EC 126.96.36.199).
Alpha diversity of microbial communities was calculated using the diversity function, with richness and Shannon’s diversity (H’) used as the indices [77, 78]. To analyze beta diversity among samples, analysis of Bray-Curtis dissimilarities was calculated using the relative abundance of OTUs and was visualized using non-parametric multidimensional scaling (NMDS) with R (ggplot package). The goodness of fit for the data was determined by the stress of the non-parametric fit [78, 79]. Significance of community composition differences among classified sample groups was determined by analysis of similarities (ANOSIM) and mrpp) [77, 79]. For analysis of beta diversity without the Salmonella OTU (Additional file 4: Figure S2), the dominant Salmonella OTU was removed from Additional file 1 and relative abundance was recalculated for each OTU within each sample by normalizing to the remaining sample relative abundance. An NMDS was generated as described above with this new OTU table. Beta dispersion was calculated using the betadisp command with vegan package in R, while significance of dispersion was tested via anova. For all R commands regarding diversity measures, see Additional file 9. All above analyses were done using the vegan package in R.
Day 15 fecal samples were analyzed using linear discriminant analysis effect size (LEfSe) . Linear discriminant effect size (LEfSe) analysis was performed at the genus level to find features (genera) differentially represented between defined groups. DSS, low-responder, and high-responder groups were compared individually to the control, and all groups were compared together. LEfSe combines the standard tests for statistical significance (Kruskal-Wallis test and pairwise Wilcoxon test) with linear discriminate analysis . It ranks features by effect size, which puts features that explain most of the biological difference at top. LEfSe analysis was performed at the α value of 0.05 for the Kruskal-Wallis test and the threshold of 2 on the logarithmic LDA score for discriminative features. A heat map of discriminant features was generated using heatmap function in the statistics package in R. Side panels of key taxa were generated in R. All R scripts used to generate Fig. 4 are included as an Additional file 9.
Weighted correlation network analysis (WGCNA) was used to generate OTUs that were positively and negatively correlated to the Salmonella OTU among day 15 samples in the Salmonella treatment group (high and low responders) using the WGCNA package in R . A Kruskal–Wallis test was used to compare the relative abundance of distinct taxonomic units and chemical metadata between treatment groups. Significance claimed in the text refers to a p value less than 0.05, unless noted otherwise. We used false discovery rate (FDR) adjusted p values to control for multiple comparison false positives, data included in Additional file 10 .
Inflammation marker quantification
Lipocalin-2 levels were measured in fecal sample supernatants using the Duoset murine Lcn-2 ELISA kit (R&D Systems, Minneapolis, MN). This method has been shown to detect low-grade inflammation and severe colitis, and compared to histopathology, lipocalin is more sensitive for detecting low-grade inflammation [32, 33]. Briefly, frozen fecal samples were reconstituted in PBS containing 0.1% Tween 20 (100 mg/ml) and vortexed for 20 min. This homogenous fecal suspension was then centrifuged for 10 min at 12,000 rpm and 4 °C.
Short-chain fatty acid quantification
To quantify short-chain fatty acids (SCFA), scraped cecal contents from five mice in the control group, five in the DSS group, and ten in the Salmonella infected group were collected and stored at −80 °C. After being lyophilized, cecal content dry weights were recorded and contents were ground on ice. Although lyophilization may reduce the total SCFA content, we selected this method to ensure equal loading of dry weight. Additionally, we conservatively report the relative SCFA amounts in one treatment group versus another rather than absolute values. Cecal contents with 3.8 to 8 mg dry weight for different samples were each transferred into a new 1.5-ml centrifuge tube, followed by the addition of 500 μl chilled methanol (Fisher Scientific) and 300 μl water (Fisher Scientific) spiked with 1.6 nmol [13C]-F-Asn. After being vortexed and centrifuged at 14,800 g for 1 h, the supernatant was aliquoted into four new 1.5-ml centrifuge tubes, frozen and lyophilized. An aliquot of each sample was re-suspended in 100 μl water, followed by filtration with a 0.2-μm PTFE filter (Thermo Scientific). A chemical derivatization was performed based on a protocol from Han et al. Twenty microliters of the solution was sequentially mixed with 10 μl of 10 mM 3-nitrophenylhydrazine (3NPH) (Sigma-Aldrich) and 10 μl of 6 mM N-(3-dimethylaminopropyl)-N’-ethylcarbodiimide (EDC) hydrochloride (Thermo Scientific) with 0.3% pyridine (Sigma-Aldrich). The reaction system was incubated at 40 °C for 2 h and cooled on ice for 1 min before dilution with 60 μl water. The solution was further diluted ten times with water before being injected for liquid chromatography-mass spectrometry analysis. A nanoACQUITY Ultra Performance Liquid Chromatography (UPLC) system (Waters, Milford, MA, USA) with a UPLC HSS T3 column (Waters, 75 μm × 100 mm, 1.8 μm) was coupled to a triple quadrupole mass spectrometer (Waters Xevo TQ-S) for SCFA analysis. Buffer A, 0.1% formic acid (Thermo Scientific) in water with 10% acetonitrile (Fisher Scientific), and buffer B, 0.1% formic acid in acetonitrile (Fisher Scientific), were used as mobile phases for gradient separation, which started with 100% A for 1 min at a flow rate of 0.8 μl/min and then followed by gradient: 1–5 min, 100–50% A; 5–7 min, 50–0% A; 7–10 min, 0% A; 10–12 min, 0–100% A; 12–30 min, 100% A. The mass spectrometer was operated in positive ion nano-electrospray ionization mode (nano-ESI+) with a capillary voltage of 3 kV, source temperature 70 °C, cone voltage 2 V and source offset 2 V. The gas flow rate for the collision cell was 0.15 ml/min. Transitions m/z 196 → 137, m/z 210 → 137, and m/z 224 → 137 with collision energy 20 eV were used for quantification of acetate, propionate, and butyrate, respectively, in multiple-reaction-monitoring mode. Another transition for each acid (m/z 196 → 138, m/z 210 → 138, m/z 224 → 138, respectively) was used for validation of quantification of acetate, propionate, and butyrate, respectively, and gave values within 14% of the first set of transitions. Standard curves were made by spiking derivatized acetic acid (Fisher Scientific), propionic acid (Acros Organics), and butyric acid (Acros Organics) into 1000-time diluted pooled cecal content extraction solutions from the high responder group and running the same LC-MS/MS analysis. Skyline-daily (v 3.5, MacCoss Lab, Department of Genome Sciences, University of Washington, Seattle, WA, USA) was used for calculating the peak area of transitions .
M.A.B., A.S.D., J.W., B.A.O., V.H.W., B.M.A., and K.C.W. are partially supported by funding from the NIH NIAID (RO1AI116119). The authors thank Jordan Angle for guidance using WGCNA computational methods. Sequencing was done at Argonne National Lab, and authors would like to thank Sarah Owens specifically. In addition, we would like to thank Jimmy John for making the preparation of this manuscript possible.
This work was supported by NIH NIAID (RO1AI116119) awarded to V.H.W., B.M.A., and K.C.W.
Availability of data and materials
The sequence data supporting the results of this article are available in the National Center of Biotechnology Information (NCBI) under Bioproject number PRJNA348350. All sequencing outputs including the OTU tables and fasta files are included in the additional information.
VHW, BMA, and KCW designed the study. MAB, ASD, JFG, and BMA performed mouse experiments and collected fecal and cecal samples. JW and VHW quantified short-chain fatty acids. ASD and BAO quantified lipocalin-2 using ELISA. MAB and BAO performed sample processing and DNA extractions. MAB, LMS, RAW, and KCW performed microbial community analyses. MAB, BMA, and KCW drafted the manuscript. Constructive edits of the manuscript were provided by LMS and RAD. All authors reviewed the results and approved the manuscript.
The authors declare that they have no competing interests.
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- Guibourdenche M, Roggentin P, Mikoleit M, Fields PI, Bockemühl J, Grimont PA, Weill F-X. Supplement 2003–2007 (No. 47) to the white-Kauffmann-Le minor scheme. Res Microbiol. 2010;161(1):26–9.View ArticlePubMedGoogle Scholar
- Sánchez-Vargas FM, Abu-El-Haija MA, Gómez-Duarte OG. Salmonella infections: an update on epidemiology, management, and prevention. Travel Med Infect Dis. 2011;9(6):263–77.View ArticlePubMedGoogle Scholar
- LaRock DL, Chaudhary A, Miller SI. Salmonellae interactions with host processes. Nat Rev Microbiol. 2015;13(4):191–205.View ArticlePubMedPubMed CentralGoogle Scholar
- Faber F, Tran L, Byndloss MX, Lopez CA, Velazquez EM, Kerrinnes T, Nuccio S-P, Wangdi T, Fiehn O, Tsolis RM. Host-mediated sugar oxidation promotes post-antibiotic pathogen expansion. Nature. 2016;534(7609):697-9.Google Scholar
- Rivera-Chávez F, Zhang LF, Faber F, Lopez CA, Byndloss MX, Olsan EE, Xu G, Velazquez EM, Lebrilla CB, Winter SE. Depletion of butyrate-producing clostridia from the gut microbiota drives an aerobic luminal expansion of Salmonella. Cell Host Microbe. 2016;19(4):443–54.View ArticlePubMedPubMed CentralGoogle Scholar
- Sekirov I, Tam NM, Jogova M, Robertson ML, Li Y, Lupp C, Finlay BB. Antibiotic-induced perturbations of the intestinal microbiota alter host susceptibility to enteric infection. Infect Immun. 2008;76(10):4726–36.View ArticlePubMedPubMed CentralGoogle Scholar
- Stecher B, Robbiani R, Walker AW, Westendorf AM, Barthel M, Kremer M, Chaffron S, Macpherson AJ, Buer J, Parkhill J. Salmonella enterica serovar typhimurium exploits inflammation to compete with the intestinal microbiota. PLoS Biol. 2007;5(10):e244.View ArticlePubMed CentralGoogle Scholar
- Thiennimitr P, Winter SE, Winter MG, Xavier MN, Tolstikov V, Huseby DL, Sterzenbach T, Tsolis RM, Roth JR, Bäumler AJ. Intestinal inflammation allows Salmonella to use ethanolamine to compete with the microbiota. Proc Natl Acad Sci. 2011;108(42):17480–5.View ArticlePubMedPubMed CentralGoogle Scholar
- Winter SE, Thiennimitr P, Winter MG, Butler BP, Huseby DL, Crawford RW, Russell JM, Bevins CL, Adams LG, Tsolis RM. Gut inflammation provides a respiratory electron acceptor for Salmonella. Nature. 2010;467(7314):426–9.View ArticlePubMedPubMed CentralGoogle Scholar
- Winter SE, Winter MG, Xavier MN, Thiennimitr P, Poon V, Keestra AM, Laughlin RC, Gomez G, Wu J, Lawhon SD. Host-derived nitrate boosts growth of E coli in the inflamed gut. Science. 2013;339(6120):708–11.View ArticlePubMedPubMed CentralGoogle Scholar
- Hapfelmeier S, Hardt W-D. A mouse model for S. typhimurium-induced enterocolitis. Trends Microbiol. 2005;13(10):497–503.View ArticlePubMedGoogle Scholar
- Barthel M, Hapfelmeier S, Quintanilla-Martínez L, Kremer M, Rohde M, Hogardt M, Pfeffer K, Rüssmann H, Hardt W-D. Pretreatment of mice with streptomycin provides a Salmonella enterica serovar Typhimurium colitis model that allows analysis of both pathogen and host. Infect Immun. 2003;71(5):2839–58.View ArticlePubMedPubMed CentralGoogle Scholar
- Coburn B, Li Y, Owen D, Vallance BA, Finlay BB. Salmonella enterica serovar Typhimurium pathogenicity island 2 is necessary for complete virulence in a mouse model of infectious enterocolitis. Infect Immun. 2005;73(6):3219–27.View ArticlePubMedPubMed CentralGoogle Scholar
- Stecher B, Macpherson AJ, Hapfelmeier S, Kremer M, Stallmach T, Hardt W-D. Comparison of Salmonella enterica serovar Typhimurium colitis in germfree mice and mice pretreated with streptomycin. Infect Immun. 2005;73(6):3228–41.View ArticlePubMedPubMed CentralGoogle Scholar
- Rivera-Chávez F, Winter SE, Lopez CA, Xavier MN, Winter MG, Nuccio S-P, Russell JM, Laughlin RC, Lawhon SD, Sterzenbach T. Salmonella uses energy taxis to benefit from intestinal inflammation. PLoS Pathog. 2013;9(4):e1003267.View ArticlePubMedPubMed CentralGoogle Scholar
- Lopez CA, Winter SE, Rivera-Chávez F, Xavier MN, Poon V, Nuccio S-P, Tsolis RM, Bäumler AJ. Phage-mediated acquisition of a type III secreted effector protein boosts growth of salmonella by nitrate respiration. MBio. 2012;3(3):e00143–00112.View ArticlePubMedPubMed CentralGoogle Scholar
- Chassaing B, Aitken JD, Malleshappa M, Vijay-Kumar M. Dextran sulfate sodium (DSS)-induced colitis in mice. Curr Protoc Immunol. 2014:15.25. 11-15.25. 14.Google Scholar
- Wirtz S, Neufert C, Weigmann B, Neurath MF. Chemically induced mouse models of intestinal inflammation. Nat Protoc. 2007;2(3):541–6.View ArticlePubMedGoogle Scholar
- Kaiser BLD, Li J, Sanford JA, Kim Y-M, Kronewitter SR, Jones MB, Peterson CT, Peterson SN, Frank BC, Purvine SO. A multi-omic view of host-pathogen-commensal interplay in Salmonella-mediated intestinal infection. PLoS One. 2013;8(6):e67155.View ArticleGoogle Scholar
- Barman M, Unold D, Shifley K, Amir E, Hung K, Bos N, Salzman N. Enteric salmonellosis disrupts the microbial ecology of the murine gastrointestinal tract. Infect Immun. 2008;76(3):907–15.View ArticlePubMedGoogle Scholar
- Deriu E, Liu JZ, Pezeshki M, Edwards RA, Ochoa RJ, Contreras H, Libby SJ, Fang FC, Raffatellu M. Probiotic bacteria reduce salmonella typhimurium intestinal colonization by competing for iron. Cell Host Microbe. 2013;14(1):26–37.View ArticlePubMedPubMed CentralGoogle Scholar
- Behnsen J, Deriu E, Sassone-Corsi M, Raffatellu M. Probiotics: properties, examples, and specific applications. Cold Spring Harb Perspect Med. 2013;3(3):a010074.View ArticlePubMedPubMed CentralGoogle Scholar
- Brugiroux S, Beutler M, Pfann C, Garzetti D, Ruscheweyh HJ, Ring D, Diehl M, Herp S, Lotscher Y, Hussain S, et al. Genome-guided design of a defined mouse microbiota that confers colonization resistance against Salmonella enterica serovar Typhimurium. Nat Microbiol. 2016;2:16215.View ArticlePubMedGoogle Scholar
- Tellez G, Pixley C, Wolfenden R, Layton S, Hargis B. Probiotics/direct fed microbials for Salmonella control in poultry. Food Res Int. 2012;45(2):628–33.View ArticleGoogle Scholar
- Higgins S, Higgins J, Wolfenden A, Henderson S, Torres-Rodriguez A, Tellez G, Hargis B. Evaluation of a Lactobacillus-based probiotic culture for the reduction of Salmonella Enteritidis in neonatal broiler chicks. Poult Sci. 2008;87(1):27–31.View ArticlePubMedGoogle Scholar
- Spees AM, Lopez CA, Kingsbury DD, Winter SE, Bäumler AJ. Colonization resistance: battle of the bugs or Menage a Trois with the host? PLoS Pathog. 2013;9(11):e1003730.View ArticlePubMedPubMed CentralGoogle Scholar
- Maltby R, Leatham-Jensen MP, Gibson T, Cohen PS, Conway T. Nutritional basis for colonization resistance by human commensal Escherichia coli strains HS and Nissle 1917 against E. coli O157: H7 in the mouse intestine. PLoS One. 2013;8(1):e53957.View ArticlePubMedPubMed CentralGoogle Scholar
- Sabag-Daigle A, Blunk HM, Gonzalez JF, Steidley BL, Boyaka PN, Ahmer BM. The use of attenuated but metabolically competent Salmonella as a probiotic to prevent or treat Salmonella infection. Infect Immun. 2016:IAI. 00250-00216.Google Scholar
- Sartor RB. Microbial influences in inflammatory bowel diseases. Gastroenterology. 2008;134(2):577–94.View ArticlePubMedGoogle Scholar
- Sobhani I, Tap J, Roudot-Thoraval F, Roperch JP, Letulle S, Langella P, Corthier G, Van Nhieu JT, Furet JP. Microbial dysbiosis in colorectal cancer (CRC) patients. PLoS One. 2011;6(1):11932–6203.View ArticleGoogle Scholar
- Kaakoush NO, Day AS, Huinao KD, Leach ST, Lemberg DA, Dowd SE, Mitchell HM. Microbial dysbiosis in pediatric patients with Crohn’s disease. J Clin Microbiol. 2012;50(10):3258–66.View ArticlePubMedPubMed CentralGoogle Scholar
- Chassaing B, Srinivasan G, Delgado MA, Young AN, Gewirtz AT, Vijay-Kumar M. Fecal lipocalin 2, a sensitive and broadly dynamic non-invasive biomarker for intestinal inflammation. PLoS One. 2012;7(9):41932–6203.View ArticleGoogle Scholar
- Raffatellu M, George MD, Akiyama Y, Hornsby MJ, Nuccio S-P, Paixao TA, Butler BP, Chu H, Santos RL, Berger T. Lipocalin-2 resistance confers an advantage to Salmonella enterica serotype Typhimurium for growth and survival in the inflamed intestine. Cell Host Microbe. 2009;5(5):476–86.View ArticlePubMedPubMed CentralGoogle Scholar
- Ríos-Covián D, Ruas-Madiedo P, Margolles A, Gueimonde M, de los Reyes-Gavilán CG, Salazar N. Intestinal short chain fatty acids and their link with diet and human health. Front Microbiol. 2016;7:185.View ArticlePubMedPubMed CentralGoogle Scholar
- Singh P, Teal TK, Marsh TL, Tiedje JM, Mosci R, Jernigan K, Zell A, Newton DW, Salimnia H, Lephart P. Intestinal microbial communities associated with acute enteric infections and disease recovery. Microbiome. 2015, 3(1):1%@ 2049-2618.Google Scholar
- Segata N, Izard J, Waldron L, Gevers D, Miropolsky L, Garrett WS, Huttenhower C. Metagenomic biomarker discovery and explanation. Genome Biol. 2011;12(6):1474–760.View ArticleGoogle Scholar
- Giloteaux L, Goodrich JK, Walters WA, Levine SM, Ley RE, Hanson MR. Reduced diversity and altered composition of the gut microbiome in individuals with myalgic encephalomyelitis/chronic fatigue syndrome. Microbiome. 2016;4(1):30.View ArticlePubMedPubMed CentralGoogle Scholar
- Ormerod KL, Wood DLA, Lachner N, Gellatly SL, Daly JN, Parsons JD, Dal’Molin CGO, Palfreyman RW, Nielsen LK, Cooper MA. Genomic characterization of the uncultured Bacteroidales family S24-7 inhabiting the guts of homeothermic animals. Microbiome. 2016;4(1):2049–618.View ArticleGoogle Scholar
- Lagkouvardos I, Pukall R, Abt B, Foesel BU, Meier-Kolthoff JP, Kumar N, Bresciani A, Martínez I, Just S, Ziegler C. The Mouse Intestinal Bacterial Collection (miBC) provides host-specific insight into cultured diversity and functional potential of the gut microbiota. Nat Microbiol. 2016;1:12058–5276.Google Scholar
- O’Mahony L, O’Callaghan L, McCarthy J, Shilling D, Scully P, Sibartie S, Kavanagh E, Kirwan WO, Redmond HP, Collins JK. Differential cytokine response from dendritic cells to commensal and pathogenic bacteria in different lymphoid compartments in humans. Am J Physiol Gastrointest Liver Physiol. 2006;290(4):G839–45.View ArticlePubMedGoogle Scholar
- Petri WA, Miller M, Binder HJ, Levine MM, Dillingham R, Guerrant RL. Enteric infections, diarrhea, and their impact on function and development. J Clin Invest. 2008;118(4):1277–90.View ArticlePubMedPubMed CentralGoogle Scholar
- Riley LW, Cohen ML, Seals JE, Blaser MJ, Birkness KA, Hargrett NT, Martin SM, Feldman RA. Importance of host factors in human salmonellosis caused by multiresistant strains of Salmonella. Asian J Infect Dis. 1984;149(6):878–83.View ArticleGoogle Scholar
- Wong JMW, De Souza R, Kendall CWC, Emam A, Jenkins DJA. Colonic health: fermentation and short chain fatty acids. J Clin Gastroenterol. 2006;40(3):235–43.View ArticlePubMedGoogle Scholar
- Machiels K, Joossens M, Sabino J, De Preter V, Arijs I, Eeckhaut V, Ballet V, Claes K, Van Immerseel F, Verbeke K. A decrease of the butyrate-producing species Roseburia hominis and Faecalibacterium prausnitzii defines dysbiosis in patients with ulcerative colitis. Gut. 2013:gutjnl-2013-304833.Google Scholar
- Maslowski KM, Vieira AT, Ng A, Kranich J, Sierro F, Yu D, Schilter HC, Rolph MS, Mackay F, Artis D. Regulation of inflammatory responses by gut microbiota and chemoattractant receptor GPR43. Nature. 2009;461(7268):1282–6.View ArticlePubMedPubMed CentralGoogle Scholar
- Wrighton KC, Thomas BC, Sharon I, Miller CS, Castelle CJ, VerBerkmoes NC, Wilkins MJ, Hettich RL, Lipton MS, Williams KH, et al. Fermentation, hydrogen, and sulfur metabolism in multiple uncultivated bacterial phyla. Science. 2012;337(6102):1661–5.View ArticlePubMedGoogle Scholar
- Tracy BP, Jones SW, Fast AG, Indurthi DC, Papoutsakis ET. Clostridia: the importance of their exceptional substrate and metabolite diversity for biofuel and biorefinery applications. Curr Opin Biotechnol. 2012;23(3):364–81.View ArticlePubMedGoogle Scholar
- Paredes CJ, Alsaker KV, Papoutsakis ET. A comparative genomic view of clostridial sporulation and physiology. Nat Rev Microbiol. 2005;3(12):969–78.View ArticlePubMedGoogle Scholar
- Jiang W, Wu N, Wang X, Chi Y, Zhang Y, Qiu X, Hu Y, Li J, Liu Y. Dysbiosis gut microbiota associated with inflammation and impaired mucosal immune function in intestine of humans with non-alcoholic fatty liver disease. Sci Rep. 2015;5:8096.View ArticlePubMedPubMed CentralGoogle Scholar
- Willing BP, Dicksved J, Halfvarson J, Andersson AF, Lucio M, Zheng Z, Järnerot G, Tysk C, Jansson JK, Engstrand L. A pyrosequencing study in twins shows that gastrointestinal microbial profiles vary with inflammatory bowel disease phenotypes. Gastroenterology. 2010;139(6):1844–54.View ArticlePubMedGoogle Scholar
- Berry D, Schwab C, Milinovich G, Reichert J, Mahfoudh KB, Decker T, Engel M, Hai B, Hainzl E, Heider S. Phylotype-level 16S rRNA analysis reveals new bacterial indicators of health state in acute murine colitis. ISME J. 2012;6(11):2091–106.View ArticlePubMedPubMed CentralGoogle Scholar
- Vital M, Gao J, Rizzo M, Harrison T, Tiedje JM. Diet is a major factor governing the fecal butyrate-producing community structure across Mammalia, Aves and Reptilia. ISME J. 2015;9(4):832–43.View ArticlePubMedGoogle Scholar
- Vital M, Howe AC, Tiedje JM. Revealing the bacterial butyrate synthesis pathways by analyzing (meta) genomic data. MBio. 2014;5(2):e00889–00814.View ArticlePubMedPubMed CentralGoogle Scholar
- Nagai F, Morotomi M, Watanabe Y, Sakon H, Tanaka R. Alistipes indistinctus sp. nov. and Odoribacter laneus sp. nov., common members of the human intestinal microbiota isolated from faeces. Int J Syst Evol Microbiol. 2010;60(6):1296–302.View ArticlePubMedGoogle Scholar
- Shkoporov AN, Chaplin AV, Khokhlova EV, Shcherbakova VA, Motuzova OV, Bozhenko VK, Kafarskaia LI, Efimov BA. Alistipes inops sp. nov. and Coprobacter secundus sp. nov., isolated from human faeces. Int J Syst Evol Microbiol. 2015;65(12):4580–8.View ArticlePubMedGoogle Scholar
- Liévin-Le Moal V, Servin AL. Anti-infective activities of lactobacillus strains in the human intestinal microbiota: from probiotics to gastrointestinal anti-infectious biotherapeutic agents. Clin Microbiol Rev. 2014;27(2):167–99.View ArticlePubMedPubMed CentralGoogle Scholar
- Borchers MT, Carty MP, Leikauf GD. Regulation of human airway mucins by acrolein and inflammatory mediators. Am J Physiol Lung Cell Mol Physiol. 1999;276(4):L549–55.Google Scholar
- van Passel MWJ, Kant R, Zoetendal EG, Plugge CM, Derrien M, Malfatti SA, Chain PSG, Woyke T, Palva A, de Vos WM. The genome of Akkermansia muciniphila, a dedicated intestinal mucin degrader, and its use in exploring intestinal metagenomes. PLoS One. 2011;6(3):e16876.View ArticlePubMedPubMed CentralGoogle Scholar
- Ganesh BP, Klopfleisch R, Loh G, Blaut M. Commensal Akkermansia muciniphila exacerbates gut inflammation in Salmonella Typhimurium-infected gnotobiotic mice. PLoS One. 2013;8(9):e74963.View ArticlePubMedPubMed CentralGoogle Scholar
- Bassett SA, Young W, Barnett MPG, Cookson AL, McNabb WC, Roy NC. Changes in composition of caecal microbiota associated with increased colon inflammation in interleukin-10 gene-deficient mice inoculated with Enterococcus species. Nutrients. 2015;7(3):1798–816.View ArticlePubMedPubMed CentralGoogle Scholar
- Swidsinski A, Dörffel Y, Loening-Baucke V, Theissig F, Rückert JC, Ismail M, Rau WA, Gaschler D, Weizenegger M, Kühn S. Acute appendicitis is characterized by local invasion with Fusobacterium nucleatum/necrophorum. Gut. 2009: 2009.191320.Google Scholar
- Schneeberger M, Everard A, Gómez-Valadés AG, Matamoros S, Ramírez S, Delzenne NM, Gomis R, Claret M, Cani PD. Akkermansia muciniphila inversely correlates with the onset of inflammation, altered adipose tissue metabolism and metabolic disorders during obesity in mice. Sci Rep. 2015;5:16643.View ArticlePubMedPubMed CentralGoogle Scholar
- Reichardt N, Duncan SH, Young P, Belenguer A, McWilliam Leitch C, Scott KP, Flint HJ, Louis P. Phylogenetic distribution of three pathways for propionate production within the human gut microbiota. ISME J. 2014;8(6):1323–35.View ArticlePubMedPubMed CentralGoogle Scholar
- Hosseini E, Grootaert C, Verstraete W, Van de Wiele T. Propionate as a health-promoting microbial metabolite in the human gut. Nutr Rev. 2011;69(5):245–58.View ArticlePubMedGoogle Scholar
- Vinolo MAR, Rodrigues HG, Nachbar RT, Curi R. Regulation of inflammation by short chain fatty acids. Nutrients. 2011;3(10):858–76.View ArticlePubMedPubMed CentralGoogle Scholar
- Stecher B, Chaffron S, Käppeli R, Hapfelmeier S, Freedrich S, Weber TC, Kirundi J, Suar M, McCoy KD, von Mering C. Like will to like: abundances of closely related species can predict susceptibility to intestinal colonization by pathogenic and commensal bacteria. PLoS Pathog. 2010;6(1):e1000711.View ArticlePubMedPubMed CentralGoogle Scholar
- Liévin-Le Moal V, Servin AL. The front line of enteric host defense against unwelcome intrusion of harmful microorganisms: mucins, antimicrobial peptides, and microbiota. Clin Microbiol Rev. 2006;19(2):315–37.View ArticlePubMedPubMed CentralGoogle Scholar
- Galley J, Mackos A, Parry N, Ahmer B, Bailey M. 17. Probiotic Lactobacillus abrogates stressor-induced increases in colonic inflammation and shifts to commensal microbe abundance during pathogen challenge. Brain Behav Immun. 2014;40:e5–6.View ArticleGoogle Scholar
- Endt K, Stecher B, Chaffron S, Slack E, Tchitchek N, Benecke A, Van Maele L, Sirard J-C, Mueller AJ, Heikenwalder M. The microbiota mediates pathogen clearance from the gut lumen after non-typhoidal Salmonella diarrhea. PLoS Pathog. 2010;6(9):e1001097.View ArticlePubMedPubMed CentralGoogle Scholar
- Kitajima S, Morimoto M, Sagara E. A model for dextran sodium sulfate (DSS)-induced mouse colitis: bacterial degradation of DSS does not occur after incubation with mouse cecal contents. Exp Anim. 2002;51(2):203–6.View ArticlePubMedGoogle Scholar
- Caporaso JG, Lauber CL, Walters WA, Berg-Lyons D, Lozupone CA, Turnbaugh PJ, Fierer N, Knight R. Global patterns of 16S rRNA diversity at a depth of millions of sequences per sample. Proc Natl Acad Sci. 2011;108(Supplement 1):4516–22.View ArticlePubMedGoogle Scholar
- Solden LM, Hoyt DW, Collins WB, Plank JE, Daly RA, Hildebrand E, Beavers TJ, Wolfe R, Nicora CD, Purvine SO. New roles in hemicellulosic sugar fermentation for the uncultivated Bacteroidetes family BS11. ISME J. 2017;11(3):691–703.View ArticlePubMedGoogle Scholar
- Caporaso JG, Kuczynski J, Stombaugh J, Bittinger K, Bushman FD, Costello EK, Fierer N, Pena AG, Goodrich JK, Gordon JI. QIIME allows analysis of high-throughput community sequencing data. Nat Methods. 2010;7(5):335–6.View ArticlePubMedPubMed CentralGoogle Scholar
- Quast C, Pruesse E, Yilmaz P, Gerken J, Schweer T, Yarza P, Peplies J, Glöckner FO. The SILVA ribosomal RNA gene database project: improved data processing and web-based tools. Nucleic Acids Res. 2013;41(D1):D590–6.View ArticlePubMedGoogle Scholar
- Altschul SF, Madden TL, Schäffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 1997;25(17):3389–402.View ArticlePubMedPubMed CentralGoogle Scholar
- Markowitz VM, Chen IMA, Palaniappan K, Chu K, Szeto E, Pillay M, Ratner A, Huang J, Woyke T, Huntemann M. IMG 4 version of the integrated microbial genomes comparative analysis system. Nucleic Acids Res. 2013:560-7.Google Scholar
- Oksanen J, Kindt R, Legendre P, O’Hara B, Stevens MHH, Oksanen MJ, Suggests M. The vegan package. Community Ecol Package. 2007;10:631-7.Google Scholar
- Clarke KR. Non-parametric multivariate analyses of changes in community structure. Aust J Ecol. 1993;18(1):117–43.View ArticleGoogle Scholar
- McCune B, Grace J, Urban DL. MRPP (multi-response permutation procedures) in analysis of ecological communities. Oregon: MjM Software Design; 2002.Google Scholar
- Langfelder P, Horvath S. WGCNA: an R package for weighted correlation network analysis. BMC Bioinformatics. 2008;9(1):1.View ArticleGoogle Scholar
- Benjamini Y, Hochberg Y. Controlling the false discovery rate: a practical and powerful approach to multiple testing. J R Stat Soc B Methodol. 1995;(57):289-300.Google Scholar
- MacLean B, Tomazela DM, Shulman N, Chambers M, Finney GL, Frewen B, Kern R, Tabb DL, Liebler DC, MacCoss MJ. Skyline: an open source document editor for creating and analyzing targeted proteomics experiments. Bioinformatics. 2010;26(7):966–8.View ArticlePubMedPubMed CentralGoogle Scholar