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Table 1 Bacterial strains and plasmids used in this study

From: Colonocyte-derived lactate promotes E. coli fitness in the context of inflammation-associated gut microbiota dysbiosis

Strain or plasmid identifier

Description

Source

E. coli strains

 DH5α λpir

F−endA1 hsdR17 (r−m+) supE44 thi-1 recA1 gyrA relA1 Δ(lacZYA-argF)U189 φ80lacZΔM15 λpir

[35]

 S17-1 λpir

zxx::RP4 2-(Tetr::Mu) (Kanr::Tn7) λpir

[36]

 MP1

E. coli wild-type strain

[37]

 ST32

E. coli MP1 ΔlldD

This study

 ST44

E. coli MP1 ΔykgEFG

This study

 ST45

E. coli MP1 Δdld

This study

 Nissle 1917

E. coli Nissle 1917

[38]

 MW304

E. coli Nissle 1917 ΔlldD

This study

S. Tm strains

 IR715

Nalidixic acid-resistant derivative of Salmonella enterica serovar Typhimurium ATCC14028

[39]

 AJB715

S. Tm IR715 ΔphoN::KanR

[40]

 CG6

S. Tm IR715 ΔlldD

[41]

Plasmids

 pGP706

ori(R6K) mobRP4 sacRB Kanr

[34]

 pSW172

ori(R101) repA101ts Ampr

[23]

 pST3

Upstream and downstream regions of E. coli MP1 lldD in pGP706

This study

 pST4

Upstream and downstream regions of E. coli MP1 ykgEFG in pGP706

This study

 pST6

Upstream and downstream regions of E. coli MP1 dld in pGP706

This study

 pWSK29

ori(pSC101) lacZα CarbR

[42]

 pWSK129

ori(pSC101) lacZα KanR

[42]