Long-term effect of epigenetic modification in plant–microbe interactions: modification of DNA methylation induced by plant growth-promoting bacteria mediates promotion process
Microbiome volume 10, Article number: 36 (2022)
Soil microbiomes are considered a cornerstone of the next green revolution, and plant growth-promoting bacteria (PGPB) are critical for microbiome engineering. However, taking plant-beneficial microorganisms from discovery to agricultural application remains challenging, as the mechanisms underlying the interactions between beneficial strains and plants in native soils are still largely unknown. Increasing numbers of studies have indicated that strains introduced to manipulate microbiomes are usually eliminated in soils, while others have reported that application of PGPB as inocula significantly improves plant growth. This contradiction suggests the need for a deeper understanding of the mechanisms underlying microbe-induced growth promotion.
We showed PGPB-induced long-term plant growth promotion after elimination of the PGPB inoculum in soils and explored the three-way interactions among the exogenous inoculum, indigenous microbiome, and plant, which were key elements of the plant growth-promoting process. We found the rhizosphere microbiome assembly was mainly driven by plant development and root recruitments greatly attenuated the influence of inocula on the rhizosphere microbiome. Neither changes in the rhizosphere microbiome nor colonization of inocula in roots was necessary for plant growth promotion. In roots, modification of DNA methylation in response to inoculation affects gene expression related to PGPB-induced growth promotion, and disruptions of the inoculation-induced DNA methylation patterns greatly weakened the plant growth promotion. Together, our results showed PGPB-induced DNA methylation modifications in roots mediated the promotion process and these modifications remained functional after elimination of the inoculum from the microbiome.
This study suggests a new mechanism in which PGPB affect DNA methylation in roots to promote plant growth, which provides important insights into microbiome–plant interactions and offers new strategies for plant microbiome engineering beyond the perspective of maintaining inoculum persistence in soils.
In recent decades, host-associated microbial consortia have been one of the most profound discoveries with overwhelming importance for hosts, such as microbes growing in the gut or rhizosphere [1,2,3,4]. Soil microbiomes have been discussed as a cornerstone of the next green revolution, as plant-beneficial rhizobacteria may not only reduce dependence on environmentally unfriendly pesticides and agrochemicals, but also increase crop productivity, enabling more sustainable agriculture [5,6,7,8]. Among soil microbes, plant growth-promoting bacteria (PGPB) are key to engineering microbiomes for application in agriculture, including improving biocontrol and plant growth . Although the mechanism underlying PGPB-mediated plant growth promotion has not been completely documented, many studies have shown that PGPB can directly facilitate nutrient acquisition or modulate phytohormone levels in plants or indirectly affect plant growth by suppressing various pathogens and enhancing the immune system or improving resistance to environmental stresses .
Reports of PGPB have been increasing exponentially, providing PGPB inocula for manipulating microbiomes in natural fields. However, it is usually difficult for these inocula to survive and persist in exogenous niches due to competition from indigenous microbes and/or local soil conditions, both of which might inhibit or even eliminate the inocula in the indigenous microbial consortium . Indeed, in many studies, the inocula failed to flourish and their abundances decreased significantly after inoculation in exogenous soils [10,11,12,13,14]. Although maintaining persistence and/or sufficient abundance of inoculum for plant growth-promoting activity is not guaranteed, many studies have reported that application of PGPB as inocula significantly improved growth of different plants . The paradox of continued growth promotion after the reduction or elimination of inocula in soils raises the question of what is maintained to promote plant growth and suggests the need for a deeper understanding of the mechanisms underlying PGPB-mediated plant growth promotion.
As the three-way interactions among the exogenous inoculum, indigenous microbiome, and plant are key elements of the onset and persistence of the plant growth-promoting process, we hypothesized that the following two types of variation might function continually in this process: changes in the rhizosphere microbiome and/or epigenetic changes in plants induced by the inoculum. Introduction of bacteria into soil modifies the bacterial community composition and structure, at least in the initial phase [12, 15,16,17]. Therefore, the altered bacterial community, including many uncultured and unknown strains, might be involved in the growth-promoting process. In plants, epigenetic modifications play an important role in regulating gene expression and coping with biotic and abiotic stresses during growth and development [18,19,20,21,22,23,24,25]. Some stress-induced epigenetic modifications may be stable and heritable, becoming epigenetic memories in the process of plant adaptation to stresses [26, 27]. To date, however, few studies have demonstrated the dynamic epigenetic modifications in plants responding to growth-promoting activities [28, 29]; thus, the role of epigenetic modification in PGPB-mediated plant growth promotion remains unknown.
DNA methylation, a common epigenetic regulation, mainly occurs at stable and easily accessible cytosine residues. Changes in DNA methylation in response to stimuli can be durable, and even potentially be passed on to subsequent generations [30, 31]. In plants, cytosine methylation occurs in three distinct sequence contexts: cytosine-guanine (CG), cytosine-H-guanine (CHG), and cytosine-H-H (CHH), where H indicates adenine (A), cytosine (C), or thymine (T). Mechanisms involved in DNA methylation maintenance are well documented [32,33,34,35]. Cytosine modifications in the CG context are maintained by methyltransferase 1, those in the CHG context are maintained by chromomethylase 2 (CMT2) or 3, and those in the CHH context are maintained by domains rearranged methylase 2 or CMT2. DNA demethylation is catalyzed by a family of DNA glycosylases, including repressor of silencing 1, demeter, and demeter-like 2 and 3. Despite the enormous progress made in understanding the molecular mechanisms regulating DNA methylation in response to environmental stimuli, most studies have used model plants, particularly Arabidopsis, and thus how DNA methylation mediates the stress responses of native plants in their natural habitats is largely unknown .
Pokeweed (Phytolacca americana L.), a native perennial herb in North America, is able to flourish in many habitats and has become a common invasive alien in China. P. americana is a Mn/Cd hyperaccumulator which has great potential for bioremediation of heavy metal (HM)-contaminated soils. To facilitate the use of a HM hyperaccumulator in bioremediation, the strategy of microbe-assisted phytoremediation has been suggested . Here, we isolated two PGPB, Bacillus sp. PGP5 (Supplementary Materials) and Arthrobacter sp. PGP41 , to better implement PGPB-assisted phytoremediation of HM-contaminated environments by P. americana. Our previous study showed genetic variation is not likely to be responsible for the wide ecological distribution of P. americana while phenotypic plasticity plays a major role in its responding to different environments . As phenotypic plasticity can be mediated through epigenetic regulation [38, 39], epigenetic states may be altered when P. americana is exposed to external stimuli, such as stimulus from PGPB, thereby ultimately affecting plant growth. In this study, we investigated the influence of PGPB inocula on rhizosphere microbiome assembly during plant development, the taxonomic and functional differences between inoculated and non-inoculated rhizosphere microbiomes, and the role of DNA methylation in PGPB-mediated plant growth promotion. Inoculation with either strain significantly increased the growth of P. americana. The successional dynamics of the rhizosphere microbiome after inoculation were analyzed at both the taxonomic and functional levels by amplicon or metagenomic sequencing. Meanwhile, changes in gene expression and DNA methylation in P. americana in response to PGP5 and PGP41 inoculation were assessed by transcriptome sequencing and whole-genome bisulfite sequencing (WGBS, a whole-genome single-base resolution analysis of DNA methylation), respectively (Fig. S1). The plants were treated with zebularine (Zeb), a DNA methylation inhibitor, to further investigate the role of DNA methylation in the growth-promoting process; meanwhile, sterilized soil was used to investigate the role of the rhizosphere microbiome (Fig. S1). A model of a two-step process of interactions between the microbiome and plant was proposed; the model comprehensively documents the dynamic regulation processes of both the rhizosphere microbiome and plant. Our results provide the first evidence for the mechanism through which PGPB affect DNA methylation to promote plant growth as well as important insights into microbiome engineering, beyond the perspective of maintaining inoculum persistence in soils.
Rhizosphere microbiome assembly is mainly driven by plant development
To explore the effects of PGPB and plant development on microbiome assembly, we investigated changes in the rhizosphere microbiome over a period of 30 days after inoculation and plant cultivation treatments. High abundances of both inocula (strains PGP5 and PGP41) were detected on day 3, followed by a rapid decrease to the same level in control soils, indicating that the inocula failed to thrive in soils (Fig. 1A, B). Measures of α-diversity revealed variation in diversity over time during plant development (Fig. 1C–E). The original bacterial community (day 0) had the highest α-diversity, while the day 3 community had the lowest. From day 3 to day 30, the α-diversity of all communities increased with time. No significant change was detected between samples treated only with plant cultivation treatments (CK samples) and samples treated with a combination of plant cultivation treatments and inoculation with strain PGP5 (PGP5 samples) or strain PGP41 (PGP41 samples). The variation in α-diversity during the late phase (15–30 days) was more moderate compared to that in the early phase (0–7 days), indicating that the rhizosphere microbiome gradually became stable. Similar results were also revealed by β-diversity analyses (Fig. 1F–H). In principal coordinate analyses (PCoA) of Bray–Curtis distances of all soil samples, day 0 samples clustered together, far from the samples with plant residence (CK, PGP5, and PGP41 at different times) on the first coordinate axis (Fig. 1G). For soil samples with plant residence, the late phase samples shifted far from the early phase samples across plant developmental stages on the second coordinate axis, with overlaps on the edge between early and late phases (Fig. 1G). No separation of soil samples with different treatments was detected, indicating consistent trends of plant development-dependent shifts of the rhizosphere microbiome among treatments. In addition, the Bray–Curtis distance between rhizosphere microbiomes collected at the end (day 30) and those collected at each time point decreased with increasing plant residence time for all treatments (Fig. 1F). Furthermore, pairwise correlation analyses revealed that the rhizosphere microbiome varied dramatically at day 3, then gradually stabilized after 15 days of root development regardless of treatments, with or without inoculation (Fig. 1H). We further identified operational taxonomic units (OTUs) correlated with root residence time using a random forests model, and 13 OTUs were detected (Fig. 1I). Although the relative abundances were low in the early phase, most of the OTUs remained at high levels during the late phase (Fig. 1J). The pattern of higher levels in the late phase compared to the early phase was also detected in the samples with inoculation treatments (Fig. 1J). Together, these results indicate that the rhizosphere compartments might have a recruitment effect on the rhizosphere microbiome, which stabilized the rhizosphere microbiome after forming intimate relationships between roots and specific bacteria.
Variations in the rhizosphere microbiome induced by inocula are limited to the early phase
Although root residence and development were the main factors influencing the rhizosphere microbiome, inoculation-induced changes in the rhizosphere microbiome were detected in the early phase. In total, 58 genera with significantly different abundances were detected in PGP5-Day 3 and PGP41-Day 3 compared to CK-Day 3 (Fig. 2A, B). Hierarchical clustering based on relative abundances of these genera showed three clusters (Fig. S2). For PGP41-specific abundant genera, all day 0 samples clustered together, PGP41-Day 3 formed the second cluster, and the remaining samples grouped in a third cluster (Fig. S2). A similar clustering pattern was found for PGP5-specific genera, except that PGP5-Day 3, 7, and 15 formed the second cluster (Fig. S2). These results suggest that the differentially abundant genera in PGP41-Day 3 or PGP5-Day 3 mainly functioned in the early phase (if at all) and then were restored to CK levels in the late phase. Besides, profound differences were detected between early and late phases in co-occurrence network analyses (Fig. 2D–G and Fig. S3). In general, early phase networks were much more complex, connected, and close than late phase networks, reflected by the higher total links, average degree, and average clustering coefficient, as well as the lower average path lengths and harmonic geodesic distances (Fig. S3). In addition, there were marked differences in the networks between CK and PGP5 or PGP41 in the early phase but not in the late phase, as confirmed by the node-level features (Fig. 2F, G). Furthermore, inoculation with PGP5 or PGP41 remarkably decreased the negative correlations in the early phase, from 19.7% (CK) to 13.6% (PGP5) and 10.5% (PGP41) (Fig. 2D, E and Fig. S3). No marked difference in negative correlations was detected in the late phase, with 26.4%, 28.6%, and 27.2% for CK, PGP5, and PGP41, respectively. The increased negative correlations in the late phase compared to the early phase might result from the recruitment of specific bacteria by roots, which exclude non-related bacteria. Taken together, the results indicate the persistent effects of recruitment by roots on rhizosphere bacteria while inoculations mainly influence the rhizosphere microbiome in the early phase at the taxonomic level.
Metagenomic analyses were performed to gain insights into the functional differences between rhizosphere microbiomes. Day 3 and day 30 samples were selected to represent the early and late phases, respectively. In principal component analysis (PCA) of the functional profiles, day 3 samples were separated from the day 30 samples on the first coordinate axis, and PGP5 and PGP41 samples were separated from CK at day 3 on the second coordinate axis (Fig. 3A). However, at day 30, no separation was detected among PGP5 and PGP41 samples and CK (Fig. 3A). All genes annotated with KEGG categories showed higher variation (measured as a coefficient of variation across all samples) in day 3 samples than in day 30 samples (Fig. 3B). Significant variation was observed when only carbohydrate metabolic categories or amino acid metabolic categories were included in the analyses (Fig. 3B). For day 3 samples, totals of 55 and 77 COGs of proteins with significantly different abundances were identified in PGP5–CK and PGP41–CK comparisons, respectively (Fig. 3C–E). However, these COGs showed similar abundances between samples at day 30 (Fig. 3D, E). Furthermore, we detected no COG or KEGG categories with significantly different abundances in PGP5–CK or PGP41–CK comparisons at day 30. Together, these results indicate that functional-level variation in the rhizosphere microbiome induced by inoculation treatments was limited to the early phase.
Microbiome-induced changes in root gene expression are selectively maintained into the late phase
We used RNA sequencing (RNA-seq) to establish differential patterns of gene expression between plants grown in inoculated and non-inoculated soils in both the early and late phases. PCA showed that the two phases have widely different gene expression patterns (Fig. S4), in agreement with the results based on the rhizosphere microbiome. At day 3, totals of 20,968 and 11,825 differentially expressed genes (DEGs) were detected in the PGP5–CK and PGP41–CK comparisons, respectively (Fig. 4A), indicating a thorough change in gene expression elicited by inoculation and/or the altered rhizosphere microbiome in the early phase. Far fewer DEGs were detected at day 30, with 2825 and 1843 in the PGP5–CK and PGP41–CK comparisons, respectively. Interestingly, 37.4% and 40.6% of the DEGs at day 30 were also differently expressed at day 3 for PGP5 and PGP41, respectively (Fig. 4A). Gene Ontology (GO) enrichment analyses of DEGs showed that most of the enriched terms (64% and 76% for PGP5 and PGP41, respectively) in the late phase were also enriched in the early phase (Tables S1 and S2). These results show that the DEGs in the late phase might result from the induction of inoculation in the early phase, as no significant differences were detected between rhizosphere microbiomes at day 30. Besides, GO enrichment analyses revealed that the DEGs were involved in “hydrogen peroxide metabolic process,” “carbohydrate metabolic process,” and “cell wall organization,” which are related to the interactions between plants and microbes [40,41,42,43]. The faster and stronger response of roots in the early phase could be caused by priming, which defines the post-challenge primed state, leading to increased resistance .
The weighted gene coexpression network analysis (WGCNA) was used to determine modules of DEGs with highly correlated expression patterns and associations of root gene expression with rhizosphere microbiomes. A total of 16 coexpression modules were identified (Fig. 4B). We further investigated the relationships between the module eigengenes and traits of the rhizosphere microbiome represented by the three main principal components in PCoA of the microbial community shown in Fig. 1G. Eight modules (P < 0.05) were significantly associated with PCoA2 or PCoA3, and another five modules showed weak associations (P < 0.06) with only three modules without correlation with the three main principal components (Fig. 4B). The results indicate that the expression of DEGs in roots was highly related to the rhizosphere microbiome, particularly turquoise modules (Fig. 4C, R = 0.81, P < 0.001). Combined with results showing most DEGs and their significantly enriched functions in the late phase were also detected in the early phase, these results indicate that microbiome-induced changes in root gene expression were selectively maintained into the late phase.
Modification of DNA methylation in response to inoculation affects gene expression in roots
The genes involved in maintaining DNA methylation showed significantly different expression patterns between inoculated and non-inoculated roots in early phases but not in the late phase (Fig. S5). To test whether inoculation affected DNA methylation in roots, WGBS profiling of DNA methylation was performed. In total, 14–19 million methylated cytosines (mCs) in each sample were identified. Of these, 7–9 million mCs were in the CG context, 5–6 million were in the CHG context, and 3–4 million were in the CHH context (Fig. S6). Many more differentially methylated regions (DMRs) were identified in the CHH context compared to those in CG (9–20 fold) and CHG (2–6 fold) contexts (Fig. 5A); therefore, we only focused on DMRs in the CHH context. At day 3, we identified 1010 and 1002 DMRs in PGP5–CK and PGP41–CK comparisons, respectively, 163 (16.1%) and 108 (10.8%) of which were also identified at day 30 (Fig. S6). These results indicate that changes in DNA methylation in certain regions were maintained from the onset to the late phase during the plant–microbe interactions. In PGP5-Day 3, hypermethylated DMRs were dominant, while almost equal amounts of hypo- and hypermethylated DMRs were found in PGP41-Day 3 (Fig. 5B). However, at day 30, hypomethylated DMRs became dominant in both PGP5 and PGP41 samples (Fig. 5B). Correspondingly, hypermethylated (dominant type) DMRs in PGP5-Day 3 showed higher levels of differential transcript abundance compared to all genes (P < 0.01; Fig. 5C), whereas hypomethylated DMRs exhibited similar levels of differential transcript abundance relative to all genes (Fig. 5C). Significant differences were also detected for the dominant type of DMRs in PGP5-Day 30 (P < 0.05) and PGP41-Day 30 (P < 0.05), while the non-dominant type showed similar levels (Fig. 5C). For PGP41-Day 3, where no dominant DMR type was detected, both hypo- and hypermethylated DMRs showed differential transcript abundance (P < 0.05, Fig. 5C). The relationship between the dominant DMR type and transcript abundance suggests that DNA methylation modification induced by inoculation might be involved in the regulation of gene expression.
We further tested the influence of DMRs on gene expression. First, we analyzed the correlation between altered gene expression and DNA methylation levels based on overlapping DEGs and DMRs. At both day 3 and day 30, strong positive or negative relationships were detected between the fold changes in gene expression and DNA methylation (R > 0.7 and P < 0.001, Fig. 5E, F). Furthermore, GO analyses of DMRs in the late phase revealed enrichment of genes involved in the regulation of transcription, regulation of hormone levels, defense response, nucleotide binding, and the G protein-coupled receptor signaling pathway (Fig. 5D). All of these functions are related to gene expression regulation. In addition, among the DEGs in the turquoise module that was most significantly related to inoculation (Fig. 4C), 420 (7.6%) genes were identified as DMRs. Together, these data indicate that gene transcription was at least partially controlled by DNA methylation in the interaction between strains PGP5/PGP41 and P. americana.
Changes in DNA methylation are involved in inoculation-induced growth promotion of P. americana
The abundance of inoculated strains in rhizosphere soils and roots was calculated by quantifying the copy numbers of their 16S rRNA gene by quantitative PCR (qPCR) (Fig. S7). The inoculation significantly increased abundances of strain PGP5 and PGP41 in rhizosphere soils at early state (day 3 and 7), and the abundances of both inocula rapidly decreased to the same level in control soils. No significant difference was detected in the inoculated roots compared to controls (the non-inoculated roots) for either strain PGP5 or PGP41. The results indicated both inocula were present in the rhizosphere soils at early stage which were eliminated from rhizosphere soils at late stage and no colonization of inocula in roots. The conclusion was further verified by fluorescence in situ hybridization (FISH), green fluorescent protein (GFP)-tagged strain, and 16S rRNA gene amplification. The FISH signals of both inocula in rhizosphere soils were detected at day 3 but were absent at day 30 (Fig. S8 and S9), showing the elimination of inocula from rhizosphere soils. Similar results were got by the GFP-tagged strain (Fig. S10). Neither FISH signal (Fig. S11) nor GFP-tagged strain (Fig. S10) was detected in inoculated roots. Besides, the amplification was successful with PGP5- and PGP41-specific 16S rRNA gene primers from DNA of strain PGP5 and PGP41 respectively, but it was failed for root DNA (Fig. S11). These results suggested no colonization of inoculated strains in roots of P. americana. To test whether variation in the rhizosphere microbiome is necessary for plant growth promotion by PGPB, we used sterilized soils for plant development and inoculation treatments. No microbe was isolated from the sterilized soils (Fig. S13), showing the soils were sterilized completely. Inoculation of each strain significantly promoted plant growth in sterilized soils (Fig. S14 and S15). Together, these results indicate that neither changes in the rhizosphere microbiome nor colonization of inocula in roots is necessary for plant growth promotion.
To investigate the role of DNA methylation in inoculation-induced growth promotion of P. americana, we treated seedlings of P. americana with Zeb which is a DNA methylation inhibitor (Fig. 6A, B). After treatment, we inoculated both Zeb-treated and non-Zeb-treated P. americana, and the expressions of genes related to the inoculation-induced growth promotion were detected (Fig. 6C, D). Without Zeb treatment, genes involved in maintaining DNA methylation showing significantly different expression patterns between inoculated and non-inoculated roots in the early phase but not the late phase (Fig. 6C). However, with Zeb treatment, these genes showed misregulation of gene expression in Zeb-treated roots compared to non-Zeb-treated plants (Fig. 6C), showing that the Zeb treatment disrupted inoculation-induced DNA methylation patterns in the Zeb-treated plants. To further verify the effect of disruptions of Zeb-induced DNA methylation on expressions of genes related to inoculation-induced growth promotion, we analyzed expressions of 15 genes randomly selected from the overlapping DEGs and DMRs (Fig. 6D). Not surprisingly, misregulation of gene expression in Zeb-treated roots compared to non-Zeb-treated plants was detected among these genes. Together, the results suggested that Zeb treatments could disrupt the inoculation-induced gene expression patterns by altering the DNA methylation patterns. Consistently, with inoculation treatments, non-Zeb-treated P. americana (PGP5 and PGP41) showed significantly increased biomass of both roots and shoots compared to CK samples at day 30 (Fig. 6F), but not at day 3 (Fig. 6E), suggesting that inoculation induced plant growth promotion in the absence of Zeb treatment. More effects of inoculation on the growth of P. americana studied by both pot and filed experiments are described in Supplementary Materials (Fig. S16 and S17). However, at day 30, no significant difference in biomass was detected between Zeb + inoculation treatments (Zeb-PGP5 and Zeb-PGP41) and Zeb-only treatments (Zeb-CK) (Fig. 6F). Furthermore, the Zeb-treated control samples (Zeb-CK) and untreated CK samples showed no differences in biomass at day 30. These results indicate that the inoculum-induced plant growth promotion was greatly weakened by Zeb treatment. Besides, similar results for Zeb-treated and PGPB-inoculated plants in sterilized soils, i.e., both inoculants promoted plant growth while the Zeb treatment resulted in both inoculants losing the ability to induce plant growth promotion (Fig. S15). Together, these results suggested that the inoculation-induced growth promotion of P. americana is at least partially mediated by change in DNA methylation. Furthermore, we also detected the growth promotions of P. americana induced by inoculations at 60 days after inoculation (Fig. 6G), which suggested the altered DNA methylation elicited by the inoculum in the early phase has a long-term effect for inoculation-induced plant growth promotion after the inoculum was eliminated in soils.
Microbial communities may be functional drivers of their host plants, with roles including expanding plant metabolic capabilities, facilitating nutrient acquisition, and providing biotic/abiotic stress tolerance. In the past decade, to solve agriculture-associated problems, strategies based on plant microbiome engineering have been proposed [5,6,7,8]. However, taking plant-beneficial microorganisms from discovery to agricultural application remains challenging, as the mechanisms underlying the interactions between beneficial strains and plants in native soils are still largely unknown. As many studies have shown that strains introduced to manipulate microbiomes are usually eliminated in soils and do not persist at functionally meaningful abundances , many efforts have focused on improving the persistence of inocula, such as using genetically modified strains or functional consortia instead of a single functional strain [5, 45, 46]. In this study, we found that DNA methylation mediated the inoculation-induced plant growth promotion process and remained functional after elimination of the inoculum from the rhizosphere microbiome. These results not only provide important insights into the mechanisms underlying microbe–plant interactions but also offer strategies for plant microbiome engineering beyond the perspective of maintaining inoculum persistence in soils.
It is well documented that plants affect rhizosphere microbiomes [8, 9, 47]. The explosive development of high-throughput sequencing technologies and analysis tools greatly improves our ability to explore the influence of PGPB inoculation and plant development on the other microorganisms [48, 49]. We analyzed rhizosphere microbiome dynamics during the development of P. americana after inoculation and compared the influences of plant recruitment and PGPB inoculation. Our results indicate that root residence and recruitment are the main factors driving variation in the rhizosphere microbiome. Meanwhile, the inoculation-induced influences on the bacterial community were limited to the early phase. The reduced influence of inoculation on the rhizosphere microbiome might first result from the elimination of the inoculum in soils, after which the early influences were attenuated or eliminated in the late phase by recruitment from plant roots. In addition, both inocula promoted growth of P. americana in sterilized soils without colonizing in roots. These results indicate that neither variation in the rhizosphere microbiome nor root colonization by inocula is the main factor mediating growth promotion of P. americana.
Although several studies have demonstrated that DNA methylation plays a key role in the regulation of gene expression in plants responding to phytopathogenic bacteria [20, 23], few studies have focused on DNA methylation dynamics during the response to PGPB stimuli. In plant–phytopathogen interactions, hypomethylation has been reported in interactions between plants and phytopathogens [20, 23, 50]. In our study, hypermethylation in the early phase and hypomethylation in the late phase were predominant in plants inoculated with PGP5. Meanwhile, equal amounts of hypo- and hypermethylated DMRs were detected in the early phase in the plant–PGP41 interaction, with hypomethylation being predominant in the late phase. The conversion of dominant DMR types between the early and late phases revealed the dynamics of DNA methylation levels during plant–PGPB interactions. These results suggest the possibility that plants can distinguish between different types of microbes to establish different interactions based on epigenetic variation.
Methylated cytosines occur in almost the entirety of plant genomes, including gene bodies, sequences flanking gene bodies (such as upstream promoter regions and downstream untranslated regions), and transposable elements [22, 29, 51]. Although the roles of DNA methylation within gene boundaries or transposable elements in regulation of gene expression have been frequently documented, the functional roles of DNA methylation in gene bodies are still unknown . In Arabidopsis, one-third of methylated genes occur in transcribed regions, suggesting that methylated genes may account for a large proportion of gene-coding regions in plants . In addition, by mapping DNA methylation in the Arabidopsis genome, an interwoven relationship between gene transcription and DNA methylation has been proposed: transcription strongly influences gene methylation while gene methylation in turn affects transcription . Furthermore, a strong correlation between variation in transcript abundance and modification of DNA methylation in gene bodies rather than in non-genic regions has been detected . These results indicate possible functions of gene body methylation in gene expression regulation. Here, we used a non-model plant to study the epigenetic regulation of gene expression during plant–PGPB interactions in natural soils. Without an available genome, we focused only on genic methylation. Our results showed that gene expression could be influenced by DNA methylation modifications in gene bodies, and plant–PGPB interactions are involved in these modifications. In future studies, comprehensive analyses of DNA methylation in the entire genome are needed to systematically document the functional roles of DNA methylation in different regions.
Two clearly separated phases were detected during the interaction between the rhizosphere microbiome and plants. In the early phase, the rhizosphere microbiome was dramatically influenced by root residence and recruitment, while only moderate changes were detected in the late phase, suggesting that a stable root–microbiome system was formed. This result is consistent with the two-step selection process by which plants recruit rhizosphere microbes from soil: (1) root exudates improve the growth of some soil bacteria, leading to a shift of the microbial community, and (2) plant selection fine-tunes the bacterial communities thriving on the rhizoplane . However, the dynamics of plant responses to variation in the rhizosphere microbiome are lacking in the current two-step process. In this study, we complemented the process with plant response dynamics based on transcriptome and methylome analyses, which also suggested two distinct stages of root responses to the rhizosphere microbiome. Together, a model of a two-step process of interactions between the microbiome and plant mediated by DNA methylation and root recruitment was proposed (Fig. 7). Specifically, the early influence of the inoculum on the rhizosphere microbiome was attenuated in the late phase by root recruitments after elimination of the inoculum. Meanwhile, altered DNA methylation elicited by the inoculum in the early phase might regulate gene expression after the inoculum was eliminated in the late phase, showing a long-term effect. The model comprehensively documents the dynamic regulation processes of both the rhizosphere microbiome and plant.
Our study provides intriguing insights into microbe–plant interactions and highlights the importance of DNA methylation modifications in roots in response to PGPB, presenting a new mechanism that PGPB-induced DNA methylation modification in roots promotes the plant growth. In addition, these epigenetic modifications remained functional even after elimination of the inoculum from the microbiome. These results offer new strategies for microbiome manipulation to promote plant growth through the application of PGPB. In future studies, more efforts should be taken in uncovering the detailed process of PGPB-induced modifications of DNA methylation at early stage to document whether and which specialized molecules/metabolites produced by PGPB can modulate root DNA methylation, which would be very useful for the commercialization of PGPB in field applications.
Materials and methods
Plant, PGPB, and soil materials
The soil used in this study was surface soil (0–15 cm depth) collected from Nanjing (32° 09′ N, 118° 57′ E), China. To remove plant debris and stones, the soil samples were passed through a 4-mm sieve. The samples were transported to the laboratory and stored at 4°C until further use.
Two PGPB strains, Bacillus sp. PGP5 and Arthrobacter sp. PGP41, were used in this study. The strains were separately incubated in Luria-Bertani broth (LB) at 150 rpm and 30°C for 24 h. The cells were harvested, washed three times with sterile water, and resuspended in sterile 0.9% NaCl solution. The cell suspension was adjusted to a final optical density at 600 nm of 1.0 (corresponding to approximately 0.29 × 109 and 1.47 × 109 CFU/mL for strains PGP5 and PGP41, respectively).
To eliminate effects associated with genetic variations of plants on the plant–microbiome interactions, isogenic pokeweeds were used in this study. Approximately 200 seeds of P. americana were firstly harvested from one single plant near a lead and zinc ore smeltery in Jishou, Hunan Province, China (28° 17′ N, 109° 45′ E ) to eliminate effects associated with genetic variations. Then, these seeds were planted in pots and placed in a greenhouse with a 13/11 h day/night photoperiod at 25°C to eliminate maternal effects. The harvested seeds from greenhouse were used in our following experiments. The obtained P. americana seeds were soaked in concentrated sulfuric acid for 10 min, followed by a thorough water rinse, and then sown in vermiculite. After germination, the seedlings were transferred to a vessel containing 1.2 L of Hoagland nutrient solution. Seedlings of P. americana at the four-leaf stage were inoculated with the two PGPB strains. The inoculation was performed in separate pots (upper caliber, 14 cm; lower caliber, 10.5 cm; depth, 11.5 cm) with 1 kg soil in each pot. The roots were first dipped in the inoculum (OD600 = 1.0) or sterile water (control) for 5 min and then immediately transferred to pots. A 50-mL suspension of the inoculum was added to soil samples in each pot. Pots were randomly placed in a greenhouse with a 13/11 h day/night photoperiod at 25°C.
The experiment included two inoculated treatments and one non-inoculated control treatment (Fig. S1). The treatments were as follows: PGP5 (inoculation with strain PGP5), PGP41 (inoculation with strain PGP41), and CK (same volume of sterile water added to soil instead of inoculum). All treatments were performed in triplicate. At 0, 3, 7, 15, 21, and 30 days after inoculation, the plants and rhizosphere soils were sampled. Roots were collected and gently shaken to remove the loosely adhered soil, after which the rhizosphere soil samples were collected by removing the remnant soil with a fine sterile brush. Samples were frozen in liquid nitrogen and stored at –80°C until further analyses.
Sterilized soil was used to investigate the role of the rhizosphere microbiome in the growth-promoting process (Fig. S1). All the treatments were the same as mentioned above except for soils which were sterilized (121°C, 2 h). Besides, seedlings of P. americana were treated with Zeb to investigate the role of DNA methylation in the process inoculation-induced growth promotion (Fig. S1). P. americana seedlings were grown in Hoagland nutrient solution containing Zeb (3 μM) for 7 days before transfer to pots for further inoculation treatments. Control plants were treated with distilled water and cultivated under the same conditions. The inoculation treatments were the same as mentioned above. To monitor the presence of the inoculant strains in rhizosphere soils and roots, the combination of qPCR, FISH, GFP-tagged strain, and 16S rRNA gene amplification were used. The detailed methods are described in Supplementary Materials.
16S rRNA amplicon sequencing
In total, 54 sequencing libraries were constructed, and the details of sequencing library construction are described in Supplementary Materials. The Illumina raw reads were quality-filtered following previously described criteria . Chimeric sequences were identified and removed using UCHIME . The OTUs were analyzed using the UPARSE pipeline  and sequences were assigned to OTUs at a 3% dissimilarity cutoff. The Ribosomal Database Project classifier  was used for taxonomic annotation of each OTU at the 70% threshold. The α- and β-diversity were analyzed using the QIIME pipeline .
Metagenomic analyses were used to infer differences in the functional potential of the rhizosphere microbiome with and without inoculation. Soil samples were collected in the early (day 3) and late (day 30) phases for metagenomic analyses. Metagenomic shotgun sequencing was performed at Biozeron Biotechnology Co. on the Illumina HiSeq 2500 platform with 150 bp paired-end technology. Raw reads were filter-trimmed using Trimmomatic  to remove adapter sequences and low-quality reads, and the resulting clean reads were used for further analyses. Functional annotation was performed by comparing clean reads to the clusters of orthologous groups (COG) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases.
RNA sequencing and data analyses
Roots were sampled at 3 and 30 days after inoculation for RNA-seq analyses. The details of sequencing library construction are described in Supplementary Materials. After removing adapters and low-quality reads with Trimmomatic , the remaining reads were assembled de novo using Trinity  with default parameters. Annotation of unigenes was performed by BLASTx searches against the NCBI non-redundant, Swiss-Prot, KEGG, and COG protein databases. Gene expression was obtained using RSEM  and further identification of DEGs was performed on the basis of fragments per kilobase of transcript per million mapped reads (FPKM) values using Cuffdiff  with a false discovery rate (FDR) < 0.05 and an absolute of fold change ≥ 2.
Bisulfite DNA sequencing and data analyses
The details of sequencing library construction are described in Supplementary Materials. The clean reads were mapped to the reference unigenes obtained by RNA-seq using BSMAP . The uniquely mapped reads were reserved to determine the cytosine methylation using a previously described method . For DMR calling, only cytosines covered by at least four reads in a library were used. DMRs were searched using 200-bp bins with a 50-bp step size. Then, Fisher’s exact test was carried out and the P values were adjusted using the Benjamini–Hochberg method. Bins with an FDR < 0.05 and a fold change > 1.5 in the methylation level were retained for further analyses. Within selected bins, each cytosine was subjected to Fisher’s exact test and defined as a differentially methylated cytosine (DMC) if the following criteria were met: P < 0.01 and fold change ≥ 2 with absolute methylation differences of 0.4, 0.2, and 0.1 for CG, CHG, and CHH contexts, respectively. The bins containing at least seven DMCs were retained. Finally, neighboring DMRs separated by no more than 100 bp were joined together into a larger DMR.
Quantitative reverse transcription PCR (qRT-PCR)
RNA was reverse-transcribed using the PrimeScript RT reagent kit with gDNA Eraser (Takara Bio). The amplifications were carried out using TB Green Premix Ex Taq (Tli RNaseH Plus) kit (Takara Bio). qRT-PCR was performed on an ABI StepOnePlus real-time PCR system. Actin and beta-tubulin were used as endogenous controls. The relative gene expression levels were calculated using the 2–∆∆Ct method . The sequences of the primers used for qRT-PCR are listed in Table S3.
The PCoA was used to ordinate the microbial community structure by principal coordinates using a Bray–Curtis dissimilarity matrix calculated from the taxonomic abundance matrices. The PCA was used to visualize the community structure using the functional abundance matrices. The PCoA and PCA plots were generated using the vegan package  in R. The linear discriminate effect size (LEfSe) program  was used to explore the most discriminating OTUs among treatments. Alpha = 0.05 was used in the Wilcoxon rank sum test, and the log value for linear discriminant analyses was set to < 2.0. Differentially abundant COG and KEGG functional entries were determined with the DESeq2 package  with criteria of P < 0.05 and fold change ≥ 1.5. A random forests approach was used to identify marker OTUs discriminating plant developmental times using the randomForest package  in R. One thousand iterations were used to determine the list of OTUs ranked in order of feature importance. The optimal number of marker OTUs was identified using 10-fold cross-validation by the “rfcv” function with five repeats. Networks were constructed for each treatment based on OTU relative abundances in both early and late phases, yielding a total of six networks. The OTUs with more than five sequences per sample were retained for analyses. Network analyses were performed using the Molecular Ecological Network Analyses pipeline . Coexpression networks of 16,316 DEGs with FPKM values > 1 were performed using the weighted gene coexpression network analysis (WGCNA) package  in R. The R scripts are publicly available at https://github.com/XXH2021/Epigenetic-memory-in-plant-microbe-interactions.
Availability of data and materials
The raw sequencing reads are publicly available under NCBI BioProject accession no. PRJNA607406.
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This work was supported by grants from the National Key Research and Development Program of China (2016YFD0800803), the National Natural Science Foundation of China (31770404 and 41977120), the Fundamental Research Funds for the Central Universities (SKY202101 and KYZ201739), the China’s Postdoctoral Science Foundation (2016T90468), and the China Agriculture Research System (CARS-10-B24).
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Schematic representation of the experimental design. Fig. S2. Differential abundances of bacterial communities in inoculated and non-inoculated soils at Day 3 and Day 30. Fig. S3. Co-occurrence networks of PGP5 over time, as affected by inoculation. Fig. S4. Variation in transcript profiles between the early and late phase. Fig. S5. Heatmap based on relative transcript abundances of genes involved in maintaining DNA methylation. Fig. S6. Overview of DNA methylation levels and differences in DNA methylation among samples. Fig. S7. Comparisons of abundances of strain PGP5 and PGP41 between inoculated and non-inoculated rhizosphere soils and roots by qPCR. Fig. S8. Detection of strain PGP41 in rhizosphere soils by FISH. Fig. S9. Detection of strain PGP5 in rhizosphere soils by FISH. Fig. S10. Detection of strain PGP41 in rhizosphere soils and roots with a GFP-tagged strain. Fig. S11. Detection of strains PGP41 and PGP5 in roots by FISH. Fig. S12. Detection of strains PGP41 and PGP5 in roots by 16S rRNA gene amplification. Fig. S13. Analysis of the effectiveness of soil sterilization. Fig. S14. Comparison of inoculum-induced growth promotion of P. americana in sterilized soils vs. unsterilized soils. Fig. S15. Inoculation induced P. americana growth promotion in sterilized soils were disrupted by DNA methylation inhibitor. Fig. S16. Effects of inoculation of strain PGP5 or PGP6 on contents of Fe, K, P and Mg in P. americana. Fig. S17. Effects of inoculation of strain PGP5 or PGP41 on height, fresh weight of stems and leaves, number of inflorescences, and fresh weight of inflorescences of P. americana. Fig. S18. Neighbor-joining tree based on 16S rRNA gene sequences showing the position of strain PGP41 within the genus Bacillus. Fig. S19. Variation in the rhizosphere microbiome between Day 3 and Day 30 at the functional level. Table S1. GO enrichment analyses of PGP5 DEGs. Table S2. GO enrichment analyses of PGP41 DEGs. Table S3. Primers used in this study.
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Chen, C., Wang, M., Zhu, J. et al. Long-term effect of epigenetic modification in plant–microbe interactions: modification of DNA methylation induced by plant growth-promoting bacteria mediates promotion process. Microbiome 10, 36 (2022). https://doi.org/10.1186/s40168-022-01236-9
- DNA methylation
- Epigenetic modification
- Microbiome engineering
- Microbiome–plant interaction
- Plant growth-promoting bacteria
- Rhizosphere microbiome