Fig. 4

Bacterial communities in murine whole lung tissue show decreased variation among biological replicates compared to those in BAL fluid. Variation among lung bacterial communities of healthy mice from the same shipment was quantified using the Bray-Curtis dissimilarity index. For comparison, Bray-Curtis dissimilarity was also calculated for empty wells as a representative negative control with high variation, cecal communities as a representative body site with low variation, and tongue as a representative seed community for the lower respiratory tract. Median, IQR, and all unique pairwise comparisons (individual data points) are shown. Pairwise significance was determined by pairwise Wilcoxon test and corrected for multiple comparisons using the Benjamini-Hochberg method. Significance key: ns p > 0.05; *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001; ****p ≤ 0.0001