Expanding magnetic organelle biogenesis in the domain Bacteria

Abstract

Background

The discovery of membrane-enclosed, metabolically functional organelles in Bacteria has transformed our understanding of the subcellular complexity of prokaryotic cells. Biomineralization of magnetic nanoparticles within magnetosomes by magnetotactic bacteria (MTB) is a fascinating example of prokaryotic organelles. Magnetosomes, as nano-sized magnetic sensors in MTB, facilitate cell navigation along the local geomagnetic field, a behaviour referred to as magnetotaxis or microbial magnetoreception. Recent discovery of novel MTB outside the traditionally recognized taxonomic lineages suggests that MTB diversity across the domain Bacteria are considerably underestimated, which limits understanding of the taxonomic distribution and evolutionary origin of magnetosome organelle biogenesis.

Results

Here, we perform the most comprehensive metagenomic analysis available of MTB communities and reconstruct metagenome-assembled MTB genomes from diverse ecosystems. Discovery of MTB in acidic peatland soils suggests widespread MTB occurrence in waterlogged soils in addition to subaqueous sediments and water bodies. A total of 168 MTB draft genomes have been reconstructed, which represent nearly a 3-fold increase over the number currently available and more than double the known MTB species at the genome level. Phylogenomic analysis reveals that these genomes belong to 13 Bacterial phyla, six of which were previously not known to include MTB. These findings indicate a much wider taxonomic distribution of magnetosome organelle biogenesis across the domain Bacteria than previously thought. Comparative genome analysis reveals a vast diversity of magnetosome gene clusters involved in magnetosomal biogenesis in terms of gene content and synteny residing in distinct taxonomic lineages. Phylogenetic analyses of core magnetosome proteins in this largest available and taxonomically diverse dataset support an unexpectedly early evolutionary origin of magnetosome biomineralization, likely ancestral to the origin of the domain Bacteria.

Conclusions

These findings expand the taxonomic and phylogenetic diversity of MTB across the domain Bacteria and shed new light on the origin and evolution of microbial magnetoreception. Potential biogenesis of the magnetosome organelle in the close descendants of the last bacterial common ancestor has important implications for our understanding of the evolutionary history of bacterial cellular complexity and emphasizes the biological significance of the magnetosome organelle.

Video Abstract

Background

It was accepted widely that intracellular, membrane-bounded, metabolically functional organelles are present exclusively in eukaryotic cells and that they are absent from Bacteria and Archaea. This long-held view was revised after numerous recent discoveries of a diverse group of highly organized, membrane-enclosed organelles in the domains Bacteria and Archaea associated with specific cellular functions [1,2,3]. However, the taxonomic distribution, origin and evolution of prokaryotic organelles remain largely elusive. It is still unclear whether organelle biogenesis emerged early or late during the evolution of Bacteria and Archaea, posing problems for elucidating the evolutionary history of cellular complexity.

Magnetosomes within magnetotactic bacteria (MTB) are a striking example of prokaryotic organelles [4]. Magnetosomes consist of a lipid bilayer-bounded membrane in which nanosized, ferrimagnetic magnetite (Fe₃O₄) and/or greigite (Fe₃S₄) crystals are biomineralized and are usually arranged in chain-like structure(s) that maximize the magnetic dipole moment [5, 6] (Fig. 1). The most accepted major function of magnetic magnetosomal crystals is to produce tiny compass needles that facilitate MTB navigation to their preferred low-O₂ or anaerobic microenvironments in chemically stratified aquatic systems, a behaviour referred to as magnetotaxis or microbial magnetoreception [7]. Additional suggested functions of magnetosomal crystals include detoxification/elimination of toxic reactive oxygen species (ROS), iron sequestration and storage in which they act as an electrochemical battery, or as a gravity sensor [8,9,10] (Fig. 1a). Understanding the phylogenetic and genomic diversity of MTB could advance our understanding of the evolutionary origin of bacterial organelle biogenesis in general. Moreover, considering that magnetoreception occurs widely in both micro- and macro-organisms and that magnetosomal crystals are the only magnetoreceptors definitively characterized thus far, MTB also represent a valuable system for exploring the origin and early evolution of magnetoreception [11,12,13,14].

Fig. 1

The magnetosome in which magnetotactic bacteria (MTB) biomineralize magnetic crystals is a typical example of a bacterial organelle. a Membrane-bounded magnetosomes contain intracellular magnetic nanoparticles (Fe₃O₄ or Fe₃S₄), with typical ~ 20–150 nm sizes. Magnetic particles within MTB magnetosomes are typically organized into (a) chain-like structure(s) within the cell in order to optimize the cellular magnetic dipole moment. Functions of magnetosomes include magnetoreception [15, 16] and ROS detoxification [17, 18], both of which have been experimentally proven. Additional proposed functions, such as iron storage and sequestration, acting as an electrochemical battery or a gravity sensor, need further testing. b Representative electron micrographs of MTB cells collected in this study. The black arrows indicate magnetosome chains

MTB are distributed globally across a broad range of O₂-limited or anaerobic aquatic habitats, ranging from freshwater lakes to oceans and even to some extreme environments [19,20,21]. However, few studies have reported MTB in waterlogged soils [22, 23]. Magnetosomal biogenesis by MTB is recognized as a key component of the global iron cycle [24, 25], and MTB cells are potentially important in the biogeochemical cycling of carbon, phosphorus, nitrogen, and sulphur [9, 26,27,28]. Historically, magnetosomal biomineralization has been viewed as a specialized type of metabolism restricted to two Bacterial phyla: the Proteobacteria (Alphaproteobacteria, Deltaproteobacteria, and Gammaproteobacteria classes) and the Nitrospirae [29]. Initial attempts to explain this restricted yet scattered distribution of magnetosomal biogenesis based on a few taxa gave rise to two alternative hypotheses: polyphyletic origin in different taxonomic lineages [30], or extensive horizontal gene transfers (HGTs) [31, 32].

In recent years, the known extent of MTB diversity has undergone a significant expansion due to methodological advances, such as the successful cultivation of novel MTB strains [33,34,35,36], 16S rRNA gene-based characterization [37,38,39], genome mining of public repositories such as GenBank and IMG/ER [21, 40], and cultivation-independent surveys of magnetosome gene clusters (MGCs, which are physically clustered groups of genes that are together responsible for magnetosomal biogenesis) containing genomes [41, 42]. MTB have now been further identified in other Bacterial taxa, including the Betaproteobacteria, Zetaproteobacteria, “Candidatus Etaproteobacteria”, and “Candidatus Lambdaproteobacteria” classes of the Proteobacteria phylum, the candidate phylum Omnitrophica (previously known as the candidate division OP3), the candidate phylum Latescibacteria (previously known as the candidate division WS3), and the phylum Planctomycetes, according to the NCBI taxonomy. Analyses including data from these latter groups suggest that magnetosomal biogenesis among different Bacterial lineages has a monophyletic origin from a common ancestor, which occurred prior to divergence of the Nitrospirae and Proteobacteria phyla, or perhaps even earlier, in the last common ancestor of five MTB-containing Bacterial phyla: Proteobacteria, Nitrospirae, Omnitrophica, Latescibacteria, and Planctomycetes [19, 41, 43,44,45].

Discovery of novel MTB outside the traditionally recognized taxonomic lineages suggests that the diversity and distribution of MTB across the domain Bacteria are considerably underestimated. This raises important questions regarding the taxonomic distribution and evolutionary origin of magnetosome organelle biosynthesis. Here we present the most comprehensive metagenomic analysis available of MTB communities from geographically, physically and chemically diverse sites to evaluate two questions: (1) Are MTB taxonomically widespread among the phyla of the domain Bacteria? (2) Did the magnetosome organelle originate earlier than previously realized? Findings of this study provide novel insights into the evolutionary origins of prokaryotic organelles.

Results and discussion

Survey of MTB from diverse environments

We performed a light microscopy survey of MTB from wide-ranging environments across China and Australia (Fig. 2a, b), including sediments from freshwater lakes, ponds, rivers, creeks, paddy fields, and intertidal zones, and soils from acidic peatlands. MTB were observed microscopically in these habitats with a salinity range of < 0.1–37.0 ppt and a pH range of 4.3–8.6 (Supplementary Table 1). Unexpectedly, we find living MTB cells in acidic peatland soils (pH 4.3–5.7) with high water contents (> 60%) and organic matter contents (typically > 20%) [46,47,48]. MTB have been found broadly in diverse aquatic ecosystems, including some extreme environments such as hot springs [49, 50], saline-alkaline lakes [51], acidic lagoons and mine drainage systems [37, 52], and deep-sea sediments [53], but reports of MTB in soils are limited to a few studies that were published almost 30 years ago [22, 23]. Whether MTB can survive in waterlogged soil environments remains unresolved, and the taxonomic diversity of MTB in these environments has not been elucidated. Our finding represents, to the best of our knowledge, the first discovery of MTB in soils with relatively acidic pH. MTB in acidic peatland soils are represented mainly by deep-branching lineages such as the Nitrospirae and Omnitrophica phyla, which is markedly different from other environments (Fig. 3, discussed below). Our finding, together with the previous studies [22, 23], suggests potential widespread MTB occurrence in different types of waterlogged soils, in addition to subaqueous sediments and water bodies.

Fig. 2

Recovery of 168 MTB genomes from various environments. a Map of sampling locations (generated using the GeoMapApp 3.6.0, http://www.geomapapp.org/). Further site details are given in Supplementary Table 1. b A micrograph of MTB cells (cocci and rods) from Lake Dianchi, China, as observed under a light microscope (Olympus BX51, Olympus, Tokyo, Japan). The applied field (B) direction is from right to left. c Estimated completeness and contamination of MTB genomes reconstructed in this study. CheckM was used to estimate completeness and contamination. Of these genomes, 69 are high-quality (> 90% completeness and < 5% contamination), 64 are medium-quality (70–90% completeness and < 6% contamination), and 35 are partial (50–70% completeness and < 5% contamination) genomes. d Relative abundance of recovered MTB genomes that can be classified according to the GTDB taxonomy (database Release 04-RS89). Of the 168 recovered genomes, 34 were classified at the species level, 91 were classified at the genus level, 140 were classified at the family level, 160 were classified at the order level, and 168 could be classified at the class and phylum levels. Details are given in Supplementary Table 2

Fig. 3

Distribution of MTB genomes across Bacterial phyla and distinct environments. a The maximum-likelihood phylogenomic tree of MTB genomes and their close non-MTB relatives inferred from concatenated 120 bacterial single-copy marker proteins [54], which was constructed using IQ-TREE under the LG+I+G4 substitution model. The number in each clade refers to the number of MTB genomes reconstructed in this study. The complete tree is shown in Supplementary Figure 1. b Relative abundances of reconstructed MTB genomes in this study across different environments within each lineage. c Distribution of magnetosome genes (mam, mms, mad, and man) and feoB gene within MGCs across different lineages. d Distribution of acquired MTB genomes at the phylum level across different environments, including freshwater/marginal (< 1 ppt) and saline/brackish/marine (> 1 ppt) sediments, and soils from acidic peatland. Details are given in Supplementary Table 1

Expanded genomic diversity of MTB

Metagenomic DNA from magnetically enriched MTB was sequenced, and metagenome-assembled genomes were reconstructed with a single-sample assembly and binning strategy. Reconstructed genomes were checked manually for the presence of MGCs. From this analysis, we recover a total of 168 MGC-containing genomes with a quality score [54] above 50 (Supplementary Table 2). Of these putative MTB genomes, 69 (41%) are high-quality (with > 90% completeness and < 5% contamination), 64 (38%) are medium-quality (70–90 % complete with < 6% contamination), and 35 (21%) are partial (with 50–70% completeness and < 5% contamination) genomes (Fig. 2c and Supplementary Table 2). These genomes increase substantially the known genomic diversity of MTB, expanded from 59 (as of January 2020, Supplementary Table 3) to 227 MTB genomes, which can now be clustered into 164 unique species-level genomes based on a 95% average nucleotide identity (ANI) [55]. Of these species, 110 (67%) are reported here for the first time.

The taxonomy of the newly acquired MTB genomes is classified using a standardized phylogenomic curated taxonomy system Genome Taxonomy Database (GTDB) Toolkit [56, 57]. Around 80% of the recovered genomes cannot be assigned at the species level, and more than 45% cannot be assignable at the genus level (Fig. 2d), which confirms that many of these genomes represent previously unknown populations. The phylogenetic relationships of these genomes were further determined by phylogenomic analysis. The reconstructed genome tree is congruent with the GTDB taxonomy as shown in Fig. 3a and Supplementary Figure 1; therefore, the GTDB taxonomy is used for taxonomic classification of MTB throughout, unless otherwise noted. The corresponding NCBI taxonomy of each lineage is also given in Fig. 3a.

The 168 genomes belong to organisms from 13 distinct Bacterial phyla as defined in the GTDB taxonomy and 7 phyla according to the NCBI taxonomy (Fig. 3a and Supplementary Table 2). These genomes are recovered from several previously poorly characterized MTB groups, including 26 genomes from the Desulfobacterota phylum, 23 genomes from the Nitrospirota phylum, and 20 genomes from the Omnitrophota phylum (Fig. 3a). These novel genomes also expand substantially the representation of common MTB lineages, such as the Magnetococcia (57 genomes) and Alphaproteobacteria (13 genomes) classes of the Proteobacteria phylum. More importantly, we identify 16 genomes that are affiliated within 6 phyla that were not known until January 2020 to contain MTB, including the Nitrospinota, UBA10199, Bdellovibrionota, Bdellovibrionata_B, Fibrobacterota, and Riflebacteria phyla (Fig. 3a and Supplementary Figure 1). This expands greatly the number of Bacterial lineages associated with magnetosomal biogenesis and magnetic navigation.

To deepen our understanding of the environmental distribution patterns of MTB, the taxonomic diversity of novel MTB genomes was compared across environments. Genomes belonging to the phyla Proteobacteria and Desulfobacterota are found predominantly in both freshwater/marginal (< 1 ppt) and saline/brackish/marine (> 1 ppt) sediments, whereas genomes affiliated with the Nitrospirata and Omnitrophota phyla represent the dominant MTB groups in acidic peatland soils (Fig. 3d). MTB from the Proteobacteria, Desulfobacterota and Omnitrophota exist in all three environmental sample types, while those of the Desulfobacterota_A phylum and of the SAR324, Fibrobacterota and Planctomycetota phyla are observed exclusively in acidic peatland soils and brackish/saline/marine environments, respectively (Fig. 3b). For Proteobacteria classes, MTB genomes from the Magnetococcia and Alphaproteobacteria are found in wide-ranging environments, while those with Zetaproteobacteria and Gammaproteobacteria genomes occur solely in brackish/saline/marine environments.

Diverse MGCs across distinct taxonomic lineages

Genes for the metabolic pathway responsible for magnetosomal biogenesis have been found in contiguous gene clusters in MTB genomes [58], which are referred to as MGCs [21]. These gene clusters are not only the key to deciphering the mechanisms and evolutionary origin of magnetosome formation and magnetotaxis [5], but they also provide a wealth of gene resources for biosynthesis of membrane-bounded, single-domain magnetic nanoparticles with diverse properties for various applications [59]. The genomes acquired here contain diverse MGC types in terms of gene content and synteny (Fig. 4), which expands our knowledge of MGC diversity considerably. Discovery of various MGCs suggests the potential for diverse magnetosomal biogenesis and magnetotaxis across the domain Bacteria. The mam [60,61,62] genes that play essential roles in magnetosomal biogenesis are present in all MGCs identified here. Remarkably, we note that the feoB gene, which is responsible for iron transport into the cell, is also shared by most MTB lineages and is usually included in the MGCs (Figs. 3c and 4). Deletion of feoB in Magnetospirillum strains results in reduced magnetite biomineralization [63, 64], which indicates its potentially significant role in magnetosomal biomineralization. The mad [65, 66] and man [67] genes, which have been proposed to play important accessary functions in magnetosomal biomineralization, represent a much wider distribution across MTB genomes than previously thought: mad genes are present in the genomes of 11 phyla and man genes occur in the genomes of the Desulfobacterota (nTS_bin18 and nDJH15_bin4), Nitrospirota, and Nitrospinota (nPCR_bin9 and nNGH_bin12) phyla. The mms6 operon, which contains magnetosome genes mms6, mmsF, mms36, and mms48, controls the size and/or number of magnetic magnetosomal crystals in Magnetospirillum strains [61, 62, 68, 69], and these genes have previously only been found in the Proteobacteria phylum. Here we find that MGCs from genomes of the phyla Nitrospinota (nNGH_bin12) and SAR324 (nKLK_bin6 and nPCR_bin7) also contain mms genes (mms6 and/or mmsF). In general, the gene content and orientation of MGCs vary considerably across different taxonomic lineages but are generally conserved within the same lineage (Fig. 4), which is indicative of lineage specific MGC evolution without extensive inter-phylum HGTs and inter-class HGTs among the Proteobacteria phylum. Considering the potentially high metabolic cost of maintaining such complex gene clusters in MTB, the widely observed MGC distribution across different lineages suggests that magnetosomal biogenesis and magnetotaxis must confer selective advantages on these organisms.

Fig. 4

Representative magnetosome gene clusters (MGCs) from distinct MTB lineages recovered in this study. Genomes containing Fe₃S₄-type MGCs are highlighted with # and putative Fe₃S₄-type magnetosome genes in MGCs are denoted by *

Two types of magnetosomal mineral crystals have been identified to date: magnetite (Fe₃O₄) and greigite (Fe₃S₄). Some MTB biomineralize both minerals within the same cell [70,71,72]. Before this study, Fe₃O₄-type MGCs had been identified in all MTB lineages except for the Latescibacterota and Planctomycetota [21], while Fe₃S₄-type MGCs were only found in the Desulfobacterota (e.g., Candidatus Magnetoglobus multicellularis [73] and Candidatus Desulfamplus magnetomortis strain BW-1 [71]), Latescibacterota [40], and Planctomycetota [21] phyla. Here, most of the identified MGCs contain Fe₃O₄-type magnetosome genes (including the first identification of Fe₃O₄-type MGCs in the Planctomycetota phylum) (Fig. 4). Only a small fraction (nER2_bin1, nHLH_bin7, nN2-2_bin5, nS315_bin9, nS315_bin20, nS315_bin24, nTS_bin4, and nXX_bin1) harbor putative Fe₃S₄-type or both Fe₃O₄- and Fe₃S₄-type magnetosome genes, which suggests that Fe₃O₄-producing MTB are distributed more widely than Fe₃S₄-producing MTB in present-day habitats. The coexistence of Fe₃O₄- and Fe₃S₄-type MGCs in the same genomes of UBA10199 (nXX_bin1) and Omnitrophota (nS315_bin20 and nS315_bin24) phyla has never been reported previously, which not only indicates an unexpected phylogenetic diversity of Fe₃S₄-producing MTB, but, more importantly, challenges the traditional hypothesis regarding the origin of Fe₃S₄-producing MTB (discussed below). Thus, this study reveals that MTB in the domain Bacteria contain many more MGCs than anticipated, which captures a more complete picture of the genomic diversity of MTB. The diverse MGCs reconstructed here also represent a promising new gene resource for magnetic bio-nanoparticles that can be used to modify magnetosomal biogenesis pathways in MTB or even build new pathways in non-MTB [59].

Phylogenetic analyses of the core magnetosome proteins

A group of nine genes (mamABEIKMOPQ) was identified previously as the core magnetosome gene set shared by both Fe₃O₄- and Fe₃S₄-producing MTB [65]. The products of these genes are thought to have important functions in magnetosomal biomineralization and for magnetosome chain construction. To trace the evolutionary history of magnetosomal biogenesis, we first performed a comparative genome analysis of 83 representative high-quality MTB genomes (complete genomes and those with > 90% completeness and < 5% contamination) to identify core magnetosome genes that are defined such that > 90% of input genomes (i.e., ≥ 74 genomes) must contain these genes, which allows for missing or fragmented genes due to the incomplete nature of draft genomes. Six magnetosome genes (mamABIKMQ) meet these criteria. Among these genes, proteins encoded by mamBIMQ are identified to be essential for magnetosomal biogenesis in Magnetospirillum strains; deletion of these genes results in non-magnetotactic mutants [5, 60, 62]. Although mamA and mamK are not essential for magnetic mineral formation in Magnetospirillum species based on previous studies, the proteins they encoded are both involved in fine-tuning the magnetic dipole moment of the cell and thus magnetotaxis: MamA is responsible for magnetosome membrane assembly and MamK is involved in biomineral chain formation [74,75,76,77].

We then examined the phylogeny of each magnetosome protein of MamABKMQ across available MTB genomes (Fig. 5), with the exception of MamI due to its < 60 aa positions after alignment trimming. The resulting trees are congruent overall with the genome-based phylogeny in that they are consistent with monophyly of the major phyla. This provides good evidence that the current MGC distribution across major phyla is not due to extensive recent HGT events, but that it is due mainly to vertical inheritance. Coupled with the sharing of a common set of magnetosome genes, this strengthens the scenario of a single MGC emergence with later multiple independent losses during Bacterial diversification [12]. However, a few discrepancies must be noted, such as separation between the UBA10199 phylum of nDJH6_bin20 and nXX_bin1 (Fe₃O₄-type), separate branching of nPCR_bin9 from the other Nitrospinota lineages, clustering of the Fe₃O₄-type Planctomycetota and Omnitrophota phyla, and clustering of Desulfobacterota_A and Desulfobacterota phyla in most protein trees, and clustering of Nitrospinota (except nPCR_bin9) and SAR324 within the Proteobacteria in trees of MamB and MamM (Fig. 5 and Supplementary Figures 2 to 6). These discrepancies could have resulted from HGT events. Alternatively, considering that only limited MTB genomes are available for the UBA10199, SAR324, Nitrospinota, and Planctomycetota phyla, these groupings could also be an artefact of tree reconstruction [78]. Additional MTB genomes from these phyla will help to differentiate between these possibilities.

Fig. 5

Maximum-likelihood trees of core magnetosome proteins. Trees were inferred using the MamABKMQ found in available MTB genomes. Complete trees are shown in Supplementary Figures 2 to 6

Initial discovery of both Fe₃O₄- and Fe₃S₄-type magnetosomes and MGCs within the same cell in the Desulfobacterota phylum (Deltaproteobacteria class in the NCBI taxonomy) led to the proposal that genes for Fe₃S₄ biomineralization in magnetosomes originated in this lineage [19]. Our finding of the coexistence of Fe₃O₄- and Fe₃S₄-type MGCs in the genomes of UBA10199 and Omnitrophota phyla weakens this hypothesis. Fe₃S₄-type magnetosome proteins form a monophyletic group in all MamABMQ trees with only the exception of Fe₃S₄-type MamA of nXX_bin1 that is clustered with Nitrospirota Fe₃O₄-type counterparts with low bootstrap support (< 75%, Supplementary Fig. 2). The structures of Fe₃S₄-type protein trees generally represent their phylum-level taxonomic phylogenies (Fig. 5 and Supplementary Figures 2 to 6), which suggests that all Fe₃S₄-type magnetosome proteins likely shared a common ancestor and then evolved separately in each phylum. These findings suggest that Fe₃S₄-type MGCs likely arose before the common ancestor of the Fe₃S₄-MTB containing phyla of Desulfobacterota, Latescibacterota, Planctomycetota, UBA10199, and Omnitrophota. The Fe₃S₄-type protein cluster is not associated robustly with any other Fe₃O₄-producing MTB lineages in protein trees, which precludes determination of the evolutionary origin of Fe₃S₄-producing MTB. Considering that Fe₃O₄-type MGCs are the most widespread, with identification in all major MTB lineages, it is likely that the Fe₃O₄-types are the ancestral form of magnetosome biosynthesis and that Fe₃S₄-type MGCs arose from Fe₃O₄-type MGCs through gene cluster duplication and divergence [19]. However, both Fe₃O₄- and Fe₃S₄-type MGCs originating from an ancient unknown MGC type (Fe₃S₄ or other iron-containing biominerals) is also plausible [41].

Biogenesis of a magnetic organelle in the close descendants of the last bacterial common ancestor (LBCA)?

Magnetosomal biogenesis is now considered to have an ancient origin [19, 41, 45, 79]. Discovery in this study of divergent MGCs across various Bacterial phyla expands significantly the database of MTB genomes and allows development of a more comprehensive scenario for the origin of magnetosomal biogenesis. The phylogenies of core magnetosome proteins (Fig. 5) strengthens the notion of an ancient MGC origin, which then spread through a combination of vertical inheritance over geological times followed by multiple independent losses, HGT events, and gene/cluster duplications. This should have occurred from a more basal ancestor than previously thought prior to the divergence of all 14 known MTB-containing phyla (Fig. 3). Interestingly, these phyla are scattered within the Bacterial tree of life and their common ancestor can be traced to near the base of the tree (Supplementary Figure 7), which indicates parsimoniously that close descendants of LBCA or even the LBCA itself may have already contained ancestors of magnetosome genes and were, thus, capable of biomineralizing primitive magnetosomes.

Potential biogenesis of the magnetosome organelle in the close descendants of LBCA has two major implications. First, the LBCA could have already possessed a relatively complex subcellular organization and, thus, was not as “primitive” as is usually imagined. Early organisms on Earth are described typically as simple organisms that lacked complex subcellular structures; however, a conceptual model of a complex LBCA or even a complex last universal common ancestor (LUCA) has been proposed based on the presence of eukaryote-like features present in some members of the Planctomycetes [80, 81]. Our results imply the formation of a magnetosome organelle in the close descendants of LBCA, which supports the idea of relatively complex early organisms. On early Earth, the lack of a protective ozone layer resulted in higher harmful ultraviolet radiation than the present-day Earth, which would have been a major challenge for life in surface and shallow-water conditions [82]. The intrinsic enzyme-like properties of magnetosomal iron nanoparticles [17, 18] and the stability of Fe₃O₄ nanozyme under a wide range of temperatures (4 to 90 °C) and pH (1 to 12) [83] might have helped life to cope with environmental stresses on early Earth (e.g., detoxification of ultraviolet radiation (UVR) and free-iron-generated ROS [12]). In addition, magnetotaxis behaviour may have also protected early MTB from lethal UVR by allowing efficient geomagnetic-field-directed swimming from near surface and shallow-water microenvironments to deeper water or sediment [84]. Second, a potential magnetosome-forming LBCA implies that this feature may have been inherited by a taxonomically wide group of Bacterial phyla, although some phyla could have lost this trait during evolution. Thus, we argue that many MTB-containing lineages await discovery. During review of this manuscript, a new study was published that generated 38 novel MTB draft genomes from public databases [85], including five genomes belonging to the phyla of Elusimicrobia, Candidatus Hydrogenedentes, and Nitrospinae according to NCBI taxonomy. Future exploration of additional MTB affiliated within other Bacterial phyla, especially those near the base of the Bacterial tree [86, 87], will help to understand and trace the evolutionary origin of the magnetosome organelle.

Conclusions

We reconstruct 168 MTB genomes here, which expand substantially the genomic representation of MTB and indicate a much more widespread distribution of magnetosome organelle biogenesis across the domain Bacteria. Analysis of the core magnetosome proteins in the largest available taxonomic representation strengthens the notion of an ancient origin for magnetosome organelle biogenesis, which may date back to the base of the Bacteria. Genomes from this study will enable a better understanding of the biology and biomineralization of the magnetosome organelle and offer clues to assist in cultivation of uncultured MTB from different lineages.

Methods

Sample collection

A total of 53 sediment and soil samples were collected from a wide range of natural environments across China and Australia (Fig. 2a), including 13 sediment samples that have been described previously [41, 88] (for details, see Supplementary Table 1). Each sample was examined for the presence of living MTB by light microscopy using the hanging-drop method [89]. MTB cells from sediments and peatland soils were enriched magnetically using a “MTB trap” and enriched MTB cells were then subjected to metagenomic analyses, the detailed procedures for which are described elsewhere [45, 90]. The morphologies of enriched cells were examined using a JEM-2100 HR transmission electron microscope (JEOL, Japan) at 200 kV.

Metagenome assembly, population genome binning, and comparative genomic analyses

Metagenomic DNA from each location was sequenced on Illumina HiSeq 2000, 2500, or 4000 platforms. Sequencing data were processed through a single-sample assembly and binning strategy using a MetaWRAP pipeline [91]. The individual metagenomic datasets were assembled separately de novo using metaSPAdes (version 3.13.0) [92] with default parameters. Assembled scaffolds ≥ 2000 bp were binned separately using MetaBAT2 (version 2.12.1) [93], MaxBin2 (version 2.2.4) [94], and CONCOCT [95]. Results of three binning methods for each sample were refined using MetaWRAP’s Bin_refinement and Reassemble_bins [91]. Genome completeness and contamination were estimated with CheckM [96] using the ‘lineage_wf’ workflow. Only genomes with an estimated completeness > 50% and contamination < 10% and an estimated quality of > 50 (defined as completeness − 5 × contamination) [54] were retained. Statistics for each genome were obtained using QUAST (version 4.2) [97], including the genome length, number of scaffolds, largest scaffold, GC content, and N50. Resultant genomes were annotated using Prokka (version 1.11) [98]. Reconstructed genomes were checked manually for the presence of MGCs, followed by extensive manual verification of candidate magnetosome genes using NCBI PSI-BLAST [99]. Notably, genome sequences with > 99% ANI of previously reconstructed MTB genomes [41] from the same samples were recovered here using a different approach, which emphasizes the reproducibility of different genome-resolved metagenomic approaches. Taxonomic annotation of all acquired MTB genomes was performed using the Genome Taxonomy Database Toolkit GTDB-Tk [57] (version 0.3.2, database Release 04-RS89) with the ‘classify_wf’ function and default parameters. The genomes reconstructed here were combined with published MTB genomes (Supplementary Table 3). These genomes were dereplicated at 95% ANI for species [55] delineation using dRep [100] with ‘-sa 0.95’.

To identify the core magnetosome gene set shared by MTB in their respective genomes, only complete genomes and those with > 90% completeness and < 5% contamination are considered. These genomes were dereplicated using dRep [100] with ‘-sa 0.99’ for dereplication at 99% ANI, and finally 83 high-quality representative MTB genomes were selected. Most are draft genomes, so we define a core magnetosome protein as being present in > 90% of the input genomes (i.e., at least 74 of 83 genomes) to minimize exclusion of potential core proteins due to the incomplete nature of draft genomes. Core proteins were calculated with COGtriangles [101] using GET_HOMOLOGUES [102] and were checked manually for magnetosome proteins. MamABIKMQ protein sequences were then searched with hidden Markov models [103] (HMM) across available MTB genomes.

Phylogenetic analyses

The phylogenetic tree composed of MTB genomes and those of relatively closely related non-MTB was inferred from 120 concatenated Bacterial single-copy marker proteins [54]. Maximum-likelihood phylogeny was calculated using IQ-TREE (version 1.6.9) [104] under the LG+I+G4 substitution model with 1000 ultrafast bootstraps. The genome tree was rooted with the genome from the candidate phyla radiation (accession number LCFW00000000). For each MamABKMQ protein, sequences were aligned using MAFFT (version 7.407) [105] in ‘auto’ mode and filtered using trimAL [106] with ‘-gappyout’ option. Maximum-likelihood phylogenetic protein trees were then constructed using IQ-TREE under the TEST option for best model selection with 1000 ultrafast bootstraps. Protein trees were rooted at the midpoint. All trees were visualized using FigTree version 1.4.2 (http://tree.bio.ed.ac.uk/software/figtree/) and Interactive Tree Of Life (iTOL) v4 [107]. AnnoTree [108] was used for phylogenomic visualization of the distribution of MTB-containing phyla across the Bacterial tree of life at the taxonomic phylum level.