Fig. 2
RNA-seq data for mouse small intestine samples. a Study design for the FMT experiment. Firstly, 3-week-old ICR male mice were treated with ddH2O as the vehicle control or AOS [10 or 100 mg/kg body weight (BW)] for 3 weeks. Then the mice were maintained in regular condition for another 2 days (with dosing). Then the mice were humanely euthanized to collect small intestine luminal contents (gut microbiota). The luminal contents from each group were pooled and homogenized, diluted 1:1 in 20% sterile glycerol (saline), and frozen. Before inoculation, small intestinal content samples were diluted in sterile saline to a working concentration of 0.05 g/ml and filtered through a 70-μm cell strainer (FMT). Secondly, 3-week-old ICR male mice were injected a single dose of busulfan [40 mg/kg body weight (BW)] [23]. The following day, the mice were dosed with saline as the control or FMT via oral gavage (0.1 ml/mouse/day). Recipient mice received oral FMT inoculations once daily for 1 week. The mice were then regularly maintained for another week (5 weeks of age) and then humanely euthanized to collect samples for different analyses. b Immunofluorescence staining of Vil1 for small intestine samples. c Heatmap summary of the differentially expressed genes in the three comparisons: Sa vs. Con-FMT; Sa vs. A10-FMT; Sa vs. A100-FMT. The scale bar shows the gene expression in each group. The clusters show the groups of genes in a similar gene family. d KEGG enrichment of up-regulated genes in Sa vs. Con-FMT. e KEGG enrichment of up-regulated genes in Sa vs. A10-FMT. f KEGG enrichment of up-regulated genes in Sa vs. A100-FMT