Fig. 1

Total annotation. Annotation of the entire transcriptome assembly (including non-differentially expressed contigs). Bars representing Bacteria, Domain, Eukaryota, Viridiplantae and Fungi are selected as a useful overview of the diversity within the transcriptome. While bars represent data normalised to 100%, only ~ 65% of the sequenced reads were successfully mapped to the assembled transcriptome (so are overlooked here) and 34% of assembled contigs had no similarity to known sequences (so are again overlooked). Full annotation is provided in Additional file 4 and an interactive Krona of total annotation is available at: https://github.com/gonzalezem/Tripartite_Metatranscriptomics_article