The microbiota in bronchoalveolar lavage from young children with chronic lung disease includes taxa present in both the oropharynx and nasopharynx

Table 4 Results of SIMPER analysis comparing the microbiota in children with PBB and CSLD

Site	OTU	BLASTn (% identity)
Lower airway data	Otu00003	Moraxella catarrhalis (98)
	Otu00002	Haemophilus influenzae (99)
	Otu00004	Prevotella sp. (98)
	Otu00001	Mitis Group Streptococci (99)
	Otu00011	Neisseria lactamica (99)
	Otu00010	Neisseria sp. (99)
	Otu00005	Haemophilus haemolyticus (99)
	Otu00006	Porphyromonas sp. (99)
	Otu00009	Prevotella melaninogenica (100)
	Otu00030	Leptotrichia sp.(88)*
Upper Airway Data	Otu00003	Moraxella catarrhalis (98)
	Otu00002	Haemophilus influenzae (99)
	Otu00001	Mitis Group Streptococci (99)
	Otu00004	Prevotella sp. (98)
	Otu000012	Corynebacterium propinquum (100)
	Otu00005	Haemophilus haemolyticus (99)
	Otu00008	Staphylococcus aureus (100)
	Otu00006	Porphyromonas sp. (99)
	Otu000010	Neisseria sp. (99)
	Otu000017	Moraxella lincolnii (98)

SIMPER analysis was performed to determine the OTUs that contributed most to the observed significant dissimilarity in the microbiota between diagnostic groups. Results are shown for the 10 OTUs in the lower and upper airway data that contributed most to the differences between children with CSLD and PBB. In comparison of children with CSLD or PBB, these OTUs contributed 30 and 26 % of the observed variation in the lower and upper airway microbiota, respectively. Comparisons with control children were not done as too few control children (n = 5) had paired upper and lower airway data to allow robust assessment of explanatory variables. OTUs are listed in a decreasing order of variation explained. BLASTn identities were selected based on 100 % coverage and >98 % identity score (% identity). OTU00030 was identified by BLASTn as Leptotrichia (*), but with only 88 % identity

ISSN: 2049-2618