Fig. 4

Beta diversity and species composition of lung and gut specimens in LPS-induced ALI mice. A Bray–Curtis dissimilarity index was calculated to reflect the replicability of lung and gut specimens. ns indicated not significant, *indicated P < 0.05, and ***indicated P < 0.001. B Venn diagram analysis at the OTU level. C PCoA and D PLS-DA at phylum level. E Results of the top 20 relative abundant phyla [ranked by Lung(i)] in lung and gut specimens. The phyla in negative were generated from control groups, including empty EP tubes, the lavage solution PBS, DNA extraction buffer, and amplification buffer [n = 3 in control groups, n = 4 or 7 in BALF specimens, n = 11 in Lung(i), n = 10 in Lung(P), and n = 10 in feces]