Fig. 3

Phylogenetic tree of representative ASVs of patescibacterial genera detected in the global WWTP sample set. The tree includes representative ASVs of genera which were detected in at least one sample with more than 0.1% relative abundance by at least one V4 targeted primer pair version. The nine main patescibacterial classes are depicted by differently colored circles on the tree. The bar plot shows the log fold change (LFC) of read abundance of original vs. modified primer sets where the modified primer set detected significantly higher abundances compared to the original primers (adjusted p value < 0.05 calculated by the Wald test). The color of the outermost band represents the detection of each genus by only the original (light blue), only the modified (light pink), or both primers (grey)