Fig. 9

Dysregulated gut microbiota induced complement C3 activation and microglia-mediated aberrant synaptic pruning. A LPS in serum (SPF mice). B C3 in serum (SPF mice). C LPS in serum (GF mice). D C3 in serum (GF mice). E WB. F Immunofluorescence of microglia and CR3. G The expression of C3 protein (SPF mice). F The expression of C3 protein (GF mice). I The expression of IBA-1 protein (SPF mice). J The expression of CR3 protein (SPF mice). K The expression of IBA-1 protein (GF mice). L The expression of CR3 protein (GF mice). M The protein expression level of SYN (SPF mice). N The protein expression level of SYN (GF mice). O The protein expression level of PSD95 (SPF mice). P The protein expression level of PSD95 (GF mice). Data represent the mean ± SEM (n = 6 per group for ELISA; n = 3 per group for IF and WB). ^**P < 0.01, ^***P < 0.001 versus the Control group; ^#P < 0.05, ^###P < 0.001 versus the CUMS group