Fig. 4

Phylogenetic analysis of viruses in both clades of H. longicornis. a The prevalence with 95% confidence interval (CI) of each virus. The virus species name shown in red are newly discovered in this study. b Phylogeny of viruses in the family Hepelivirales sp. based on amino acid sequence of RNA-dependent RNA polymerase (RdRp) gene. Tree s are colored according to the genetic clade of libraries which viral sequences collected from: The red strips indicate tick samples from clade 1, the blue strips represent ticks from clade 2, and the gray strips are reference sequences. c Phylogeny of viruses in the group of Jingmenvirus based on amino acid sequence of VP1b protein. d Phylogeny of viruses in the family Peribunyaviridae based on amino acid sequence of RdRp protein. e Phylogeny of viruses between the families Solemoviridae and Tombusviridae based on amino acid sequence of RdRp protein. f Phylogeny of viruses in the family Nodaviridae based on amino acid sequence of capsid protein. g Phylogeny of viruses in the family Phenuiviridae based on amino acid sequence of nucleoprotein. h Phylogeny of viruses in the family Totiviridae based on amino acid sequence of RdRp protein. Viruses detected in this study are marked by red solid circles for ticks in clade 1 and blue solid circles for ticks in clade 2