Fig. 4
Effect of OttaBac® on microglia and adult neurogenesis in the dentate gyrus (DG) of hippocampus. a Histogram indicating the comparable total number of IBA-1+ cells in the dentate gyrus of hippocampus, among the groups. b Histogram illustrating the increased area soma of the MSPLA compared to the nMSPLA mice and the beneficial effect of OB. c–f Diagrams showing the modification of the microglia morphology towards an activated phenotype (priming) in the MSPLA group and the therapeutic effect exerted by OB on the MSOB group in terms of the following: ending radius (c), intersecting radii (d), sum and max intersection (e, f). g Histogram showing the decrease of nestin-GFP+ neural stem cells observed in the MSPLA group, when compared to their respective nMSPLA counterpart group, and the OB-dependent increase of NSCs in the MSOB group compared to MSPLA group. h Histogram showing the decrease of DCX+ immature neuronal cells (neuroblasts) observed in the MSPLA group, when compared to their respective nMSPLA counterpart group, and the OB-dependent increase of DCX+ immature neuronal cells in the MSOB group compared to MSPLA group. i Representative pictures describing the decrease of nestin-GFP+ neural stem cells (green) observed in the MSPLA group and the OB-dependent increase of NSCs in the MSOB. j Representative pictures describing the decrease of DCX+ neuroblasts (red) and the OB-dependent increase of DCX+ immature neuronal cells in the MSOB group. k Histogram showing the OB-dependent enhanced number of KI67+ cells detected in the nMSOB and MSOB groups, in comparison to their respective counterpart nMSPLA and MSPLA groups. l and m Graphs showing the OB-dependent increased proliferation of GFP+ neural stem cells observed in nMSOB and MSOB group, in comparison to their counterpart nMSPLA and MSPLA groups, respectively, and the enhanced number of proliferative DCX+ immature neuronal cells in the nMSOB and MSOB groups, in comparison to their counterpart nMSPLA and MSPLA groups, respectively. Number of animals/group = 5. Statistical significance: *p < 0.05, **p < 0.01. Two-way ANOVA analysis by Bonferroni post hoc tests. Scale bar: 100 μm