Fig. 3
L. reuteri alters the expression of major enzymes involved in microbial metabolism of tryptophan. Six-week-old C57BL/6 mice were orally gavaged with 200 µl of PBS with or without L. reuteri I5007 (109 CFU/mL) daily for 3 weeks, followed by a week of 3% DSS administration in drinking water to induce colitis (See Figure S1A for experimental scheme). Metatranscriptomics was performed with the colonic microbiota collected on day 28. A Major metabolic pathways and enzymes involved in microbial tryptophan metabolism. B PCoA plot of the Bray–Curtis distance showing the differences in the colonic microbiota function. C The differences in mRNA expression levels of major enzymes involved in microbial tryptophan metabolism among three groups of mice. Statistics was performed using DESeq2. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns, not significant