Fig. 2

FMT does not increase HGT rates following treatment, when compared with a placebo. a The percentage of HTGCs was calculated from the number of distinct recipient gene clusters involved in HGT for each FMT (n = 39) or placebo (n = 44) recipient relative to the number of distinct gene clusters on high-quality MAGs for each respective participant, at week 6. The percentage of HTGCs was compared between groups (Wilcoxon test). Each point represents a participant’s sample. Boxes represent the interquartile range (IQR) split by the median, with whiskers expanding up to 1.5 × the IQR. b The number of transfer events for each HTGC was plotted separately for the FMT-specific and placebo-specific HTGCs, for males and females. A bin width of 1 was used. HTGCs with greater than 20 transfer events were grouped under ‘20 + ’. c Counts of FMT-specific (red), shared (purple), and placebo-specific (blue) HTGCs in females and males. HGT, horizontal gene transfer; FMT, faecal microbiota transplantation; HTGC, horizontally transferred gene cluster; MAG, metagenome assembled genome; ns, not-significant (p < 0.05)