Fig. 7

Clustering and pseudotemporal trajectories identified transcriptional dynamics of porcine muscle tissues. A t-SNE analysis of 19,229 single cells from porcine muscle tissues, with 12 major cell types labeled in different colors. B Expression of representative genes in distinct cell clusters. C The proportions of the 12 major cell types in porcine muscle tissues. D Expression of IMF-enriched genes, including APOA1, SNCA, SCRG1, CDH19, CNP, and MBP genes. E t-SNE plot showing the expression of LUM, COL1A1 and THY1 in porcine FAPs. F Pseudotime single-cell trajectory reconstructed by Monocle2 for FAP subpopulation. Pseudotime was shown in a gradient from dark to light blue. G Pseudotemporal heat map showing gene expression dynamics for significant marker genes. The genes (rows) were clustered into three modules, and the cells (columns) were ordered according to pseudotime. H The differentially expressed genes between LT and LW piglets increased along with pseudotime trajectory in porcine FAP subtypes. I The differentially expressed genes between LT and LW piglets decreased along with pseudotime trajectory in porcine FAP subtypes