Fig. 6

Distinct expression patterns of genes related to cholesterol metabolism and bile secretion in porcine liver tissues. A t-SNE analysis of 18,152 single cells from porcine liver tissues, with 13 major cell types labeled in different colors. B Dot plots showing the expression of representative marker genes in porcine livers. C Graph-based re-clustering of porcine hepatocytes revealed two subpopulations: periportal and central hepatocytes. D KEGG enrichment analysis of the periportal hepatocyte-specific genes. E Dot plots showing the expression of representative marker genes in porcine periportal and central hepatocytes. F Expression patterns of breed-specific genes involved in cholesterol metabolism and bile secretion at the digestive system categories in porcine periportal hepatocytes. G Heatmap showing the differential cholesteryl esters in porcine liver tissues between LT and LW piglets