Fig. 3

Breed-specific differences involved in lipid digestion and absorption in porcine small intestinal enterocytes. A Differentially expressed genes in small intestinal epithelial cells between LW and LT piglets. The outer ring represented 1 to 18 porcine autosomal and X and Y chromosomes. The middle ring indicated the differentially expressed genes involved in lipid metabolism; red symbols represented upregulated genes in LT pigs, and green represented downregulated genes. The scatter plot in the inner ring represented all genes presented in porcine enterocytes; the size of the solid circles represented for log (Q value, 2). B Expression patterns of identified genes in fat digestion and absorption enrichment in small intestinal enterocytes between LW and LT piglets. C Lipid metabolites identified in porcine serum. PC, phosphatidylcholine; PE, phosphatidylethanolamine; PS, phosphatidylserine; PI, phosphatidylinositols; PG, phosphatidylglycerols; PA, phosphatidic acids; SM, sphingomyelines; Cer, ceramides; GlcCer, glucosyl ceramides; FA, free fatty acids; DAG, diacylglycerol; TAG, triacylglycerol; GM3, monosialodihexosyl ganglioside; GlcADG, glucuronosyl diacylglycerol; ACar, acylcarnitine; DGTS, diacylglycerol trimethyllhomoserine. D PCA analysis of the identified metabolites in porcine serum between LT and LW piglets. E Volcano plot showing the differential lipid metabolites in porcine serum between LT and LW piglets. The red circles represented increased metabolites in LT pigs, and green represented decreased metabolites. F Violin plots showing FA, DAG, and TAG concentrations in porcine serum between LT and LW piglets