Fig. 1

Construction of the single-cell landscape of porcine jejunum, colon, liver and muscle tissues. A tSNE plots showing the distribution of the main tissue-resident clusters in the tested tissues. B t-SNE analysis of 60,514 single cells from tested samples, with 20 major cell types labeled in different colors. IMF, intramuscular fat; SMMCs, smooth muscle cells; MuSCs, Muscle stem cells; DC, dendritic cells; FAP, fibroadipogenic precursors/myofibroblast. C Dot plots showing the expression of representative marker genes; dot color reflects average gene expression, and dot size represents percent of cells expressing the gene. D Heatmap representing the top 5 most variably expressed genes (row) between the given cell subpopulations (column); colors and numbers correspond to the cell types shown in B. E Proportions of the 20 major cell types in the tested tissues; JE, jejunum; RE, colon; LI, liver; MU, longissimus dorsi muscle