Fig. 7
a Relative abundance of pathway-specific mRNA and transcripts of key pathway genes in relation to total mRNA that could be mapped onto the Methanosarcina MAGs. A list of the full gene names is shown in Table S14. Mapping onto the MAGs was conducted for each mRNA dataset retrieved in triplicate from the same incubation time point (days 21, 28, and 35). The small dots indicate the mapping efficiency of each mRNA replicate dataset, while the large dot represents the mapping efficiency averaged across all three mRNA replicate datasets of a given sampling time point. The complete set of PFDR values obtained in DESeq2 analysis for all sampling time points is shown in Table S19. b Relative transcript dynamics of acetoclastic and methylotrophic methanogen pathways inferred from the competitive transcript mapping across the mix of Methanosarcina Group I to IV reference genomes. The relative mapping efficiencies were calculated based on TPM values. Data are means ± SE (n = 3). The mapping statistics are shown in Table S20