Fig. 9

Mechanistic analysis of the protective effect of LCA. GZMB (A), GZMA (E), and PRF (G) expression in CD3⁺CD8⁺ T cells was measured by flow cytometry on day 8 after PEDV infection in piglets, and the proportions of GZMB (B), GZMA (C), and PRF (D) cells were statistically analyzed (n=3). F Cell proliferation assay of CD8⁺ cells treated with 10/20 μM LCA and anti-CD3/28 for 12/24/48 h using CFSE labeling (n=3). H Cell proliferation ratio of CD8⁺ cells at 12/24/48 h (n=3). I SLA-I expression was analyzed by flow cytometry after 10/20 μM LCA treatment of IPEC-J2 cells for 12 h and was statistically analyzed (N) (n=3). O IPEC-J2 cells were treated with 10/20 μM LCA for 12 h before cell lysis and subsequent ELISA for SLA-I expression (n=3). M The expression of SLA-I was analyzed by flow cytometry in the presence and absence of SBI-115 and Gly-β-MCA in IPEC-J2 cells treated with 20 μM LCA for 12 h, and was statistically analyzed (Q) (n=3). P SLA-I expression was analyzed by flow cytometry in the presence and absence of CCDC and GW4064 in IPEC-J2 cells treated with 20 μM LCA for 12 h, and was statistically analyzed (R) (n=3). The results are presented as the means ± SD, and statistical significance was calculated by one-way ANOVA. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001