Table 2 Primers and PCR conditions used in identifying antibiotic resistance genes
Antibiotic group and gene | Primer | Nucleotide sequence(5′–3′) | Product(bp) | Annealing (ºC) | Reference | |
|---|---|---|---|---|---|---|
Sulfonamide | sul1 | sul1-F | CGGCGTGGGCTACCTGAACG | 433 | 66 | [37] |
sul1-B | GCCGATCGCGTGAAGTTCCG | |||||
sul2 | sulII-L | CGGCATCGTCAACATAACCT | 721 | |||
sulII-R | TGTGCGGATGAAGTCAGCTC | |||||
sul3 | sul3-GKa-F | CAACGGAAGTGGGCGTTGTGGA | 244 | |||
sul3-GKa-R | GCTGCACCAATTCGCTGAACG | |||||
Quinolone | qnr(A) | QnrAm-F | AGAGGATTTCTCACGCCAGG | 580 | 59 | [38] |
QnrAm-R | TGCCAGGCACAGATCTTGAC | |||||
qnr(B) | QnrBm-F | GGMATHGAAATTCGCCACTG | 264 | 59 | ||
QnrBm-R | TTTGCYGYYCGCCAGTCGAA | |||||
qnr(s) | QnrSm-F | GCAAGTTCATTGAACAGGGT | 428 | 55 | ||
QnrSm-R | TCTAAACCGTCGAGTTCGGCG | |||||
Tetracycline | tet(A) | TetA-L | GGCGGTCTTCTTCATCATGC | 502 | 63 | [37] |
TetA-R | CGGCAGGCAGAGCAAGTAGA | |||||
tet(B) | TetBGK-F2 | CGCCCAGTGCTGTTGTTGTC | 173 | 61 | ||
TetBGK-R2 | CGCGTTGAGAAGCTGAGGTG | |||||
tet(C) | TetC-L | GCTGTAGGCATAGGCTTGGT | 888 | 58 | ||
TetC-R | GCCGGAAGCGAGAAGAATCA | |||||
Aminoglycoside | strA/strB | strA-F | ATGGTGGACCCTAAAACTCT | 893 | 52 | [37] |
strB-R | CGTCTAGGATCGAGACAAAG | |||||
aac(3)IV | aac4-L | TGCTGGTCCACAGCTCCTTC | 653 | 55 | ||
aac4-R | CGGATGCAGGAAGATCAA | |||||
aadB | aadB-L | GAGGAGTTGGACTATGGATT | 208 | 52 | ||
aadB-R | CTTCATCGGCATAGTAAAAG | |||||
aphA1 | aph(3´)-Ia F | ATGGGCTCGCGATAATGTC | 634 | 58 | [39] | |
aph(3')-Ia R | CTCACCGAGGCAGTTCCAT | |||||
aphA2 | aphA2-L | GATTGAACAAGATGGATTGC | 347 | 53 | [37] | |
aphA2-R | CCATGATGGATACTTTCTCG | |||||