Fig. 5

DVF activates the LRRC19-TRAF6-MAPK/NF-κB pathway in colitis mice. A IHC staining and quantitation of LRRC19 in the colonic mucosa of DVF- or PBS-treated mice. Scale bars, 50 µm. B Confocal fluorescent analyses of the interaction between LRRC19 and TRAF2 or TRAF6 in colitis mice. Scale bars, 50 µm. C The protein levels of P-p38, P-ERK, P-NF-κB p65, and P-IκBα of DVF- or PBS-treated mice were measured by western blotting, α-tubulin was used as loading control. Relative protein levels were quantified using the Imagelab. All data are presented as mean ± SEM. *P < 0.05, ** P < 0.01, *** P < 0.001, ns, not significant. One-way ANOVA in A, two-tailed Student’s t test in C