Fig. 6

Activity alterations of Faecalibacterium prausnitzii during FMT. A Gene expression profiles of F. prausnitzii in health and disease (eggNOG genes). B The gene expression changes of acetyl-CoA pathway for butyrate biosynthesis in Faecalibacterium prausnitzii. In A, the circos plot is based on all expressed genes assigned to F. prausnitzii which were then grouped to eggNOG genes according to the ortholog annotation. Rings show (from outside to inside) the following: #2 all eggNOG genes; #3 butyrate synthesis genes (green arrow), genes involved in tryptophan metabolism (red arrow), the other genes that are involved in secondary bile acids metabolism, vitamins B1 (thiamine), B3 (nicotinate), B5 (pantothenate), B6 (pyridoxine), B7 (biotin), B9 (folate), and B12 (cobalamin) biosynthesis, are marked with bars in distinct colors; #4 log2 fold change of gene expression between health (donors) and pouchitis (patients before treatment); #5 expression level of genes in pouchitis (patients before treatment); and #6 expression level of genes in health (donors). For B, only patient’s samples before FMT, 1 week, and the last timepoint after each FMT were included. The colored box with gene names in the pathway shows the log2FC between donors and pouchitis. Red indicates the strongest upregulation in donor in comparison to pouchitis. The barplot illustrates the expression level of each gene during the treatment process. The height and color of the bar indicate the relative expression level (number of reads from that gene normalized by number of total reads from F. prausnitzii) of the gene. The blue arrow at the top of the barplot marks the first timepoint after each FMT