Fig. 1

Heatmap of taxonomic composition of the gut community during FMT based on expressed genes on the genus level. Metatranscriptome reads were mapped to an improved IGC (Integrated Gene Catalogue) database to assign functional and taxonomic information (see “Methods”) and then grouped according to genera. Abundance was normalized by the total number of mapped reads per sample. Only genera with an average relative abundance > 0.1% across all samples are shown, which represented > 98% of all taxonomically assigned reads. Right side bar, mean of relative expression of all genes for this genus across all samples; left side bar, number of expressed genes from this genus across all samples. The two column annotation bars depict the patient groups (CG, DW, and JM) and the disease status. The status “before/after ABT” indicates before or after antibiotic treatment. More details can be found in “Methods”