Skip to main content
Fig. 1 | Microbiome

Fig. 1

From: A consortia of clinical E. coli strains with distinct in vitro adherent/invasive properties establish their own co-colonization niche and shape the intestinal microbiota in inflammation-susceptible mice

Fig. 1

Workflow for novel high-throughput in vivo approach to identify highly colonizing E. coli strains and structural shifts in the mucosally adherent intestinal microbiome. Germ-free mice (inflammation-susceptible Il10−/− and inflammation-resistant WT) were gavaged with various pools of barcoded clinical E. coli strains followed by fecal microbial transplants (FMT) from pooled WT C57Bl/6 mice. We reference each experimental group by their cohort ID and treatment designation (i.e., WT + 7E.coli + FMT1). Tissue and stool were harvested from each cohort. DNA was extracted from harvested samples and used as template to PCR amplify the barcode region identifying each E. coli strain and V4 region of 16S rRNA gene for microbiome analysis. Illumina sequencing of barcoded regions revealed highly colonizing E. coli strains at the mucosally adherent niche for further pangenomic analysis to pinpoint candidate colonization factors

Back to article page

Microbiome

ISSN: 2049-2618

Contact us