Fig. 6

FMT-dd regulates bile acid synthesis via the hepatic FXR-SHP axis under normal diet and via the intestine FXR-FGF19 axis under high-fat diet. a The composition of serum primary and secondary BAs in mice. b Fecal BA profile of mice. c, d Liver and intestinal FXR immunofluorescence images. e, f The mean fluorescence intensity of the liver and intestinal FXR. g The intestinal FXR protein expression level. h The protein expression level of FXR, CYP8B1, CYP7A1, and FGF19 in the liver. i The mRNA expression of CYP7A1, CYP8, FGFR4, FXR, and SHP in the liver. j Intestinal mRNA expression of ASBT, FGF19, and FXR. k Heatmap of correlation between the abundance of microbiota significantly regulated by FMT-dd and the expression of FXR, SHP. All bar plots are presented as mean ± standard deviation. All box and whiskers plots showed the box (min to max), the median value (in the transverse line), and the whiskers (go down to the smallest value and up to the largest). All data were evaluated by the unpaired T-test in the GraphPad software. Source data are provided as a Source data file. *p < 0.05, **p < 0.01, ***p < 0.001. Fecal microbiota transplantation from dyslipidemic donors (FMT-dd); Fecal microbiota transplantation from heathy donors (FMT-hd); normal diet (ND); high-fat diet (HD); bile acids (BAs); α-Muricholic Acid (α-MCA);β-Muricholic Acid (β-MCA);cholic acid (CA); chenodeoxycholic acid (CDCA); glycocholic acid (GCA); taurochenodeoxycholic acid (TCDCA); deoxycholic acid (DCA); glycochenodeoxycholic acid (GDCA); hyodeoxycholic acid (HDCA); lithocholic acid (LCA); taurochenodeoxycholic acid (TUDCA); ursodeoxycholic acid (UDCA); farnesoid X receptor (FXR); small heterodimer partner (SHP); cholesterol 7α-hydroxylase (CYP7A1); sterol 12α-hydroxylase (CYP8B1); fibroblast growth factor 19 (FGF19); apical sodium-bile acid transporter (ASBT); FGF receptor 4 (FGFR4); sodium-taurocholate co-transporting polypeptide(NTCP)