Fig. 1
From: Application of culturomics in fungal isolation from mangrove sediments
A workflow for enrichment cultivation and in situ incubation of mangrove sediments. a Schematic diagram of the fungal enrichment cultivation method (FECM). The process incorporates the following steps: continuous incubation (0–21 days), culture-dependent research, and culture-independent research. b Handmade fungal isolation chips (FiChip) prototype for in situ incubation. The process consists of the following steps: FiChips assembly, in situ incubation, fungal isolation, and purification. A detailed protocol is described in Additional file 3