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Fig. 8 | Microbiome

Fig. 8

From: Uterine microbial communities and their potential role in the regulation of epithelium cell cycle and apoptosis in aged hens

Fig. 8

Effects of oviduct perfusion with antibiotics on uterine microbiota and microenvironment in the aged hens. a Experimental design overview. b Comparison of Shannon index of α-diversity (n = 10). c Principal coordinate analysis (PCoA) of uterine microbiota based on Bray–Curtis (n = 10). d Microbial composition at the phylum level (n = 10). e The microbial phenotype analysis based on the BugBase database (n = 10). f Effects of oviduct perfusion with antibiotics on the morphological structure of the uterus (n = 10). Scale bar = 100 μm. g Comparison of the eggshell quality and uterine microenvironment in terms of mucosal epithelial thickness, IL-1β, and MUC2 (n = 10). h Detection of cell apoptosis in uterine tissue using the TUNEL assay (n = 6). Scale bar = 50 μm. i The proportion of cell apoptosis (n = 6). j The level of B cell lymphoma-2 (Bcl-2) in the uterus mucosa (n = 10). NC, the negative control group; AB, the antibiotic group. IL-1β: Interleukin-1β; MUC2: Mucin-2

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