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Fig. 3 | Microbiome

Fig. 3

From: Uterine microbial communities and their potential role in the regulation of epithelium cell cycle and apoptosis in aged hens

Fig. 3

Changes in the microenvironment of the uterus in aged hens. a The morphological structure of young and aged uterus. Scale bar = 100 μm. b–i Comparison of the microenvironment in terms of mucosal epithelial thickness, IL-1β, immunoglobulin, MUC2, lysozyme, ovotransferrin, and pH (n = 10). j The number of bacterial DNA copies is based on the total DNA (n = 10). k The number of bacterial DNA copies based on the liquid egg (n = 10). l Quantitative analysis of apoptotic cells using the TUNEL assay (n = 8) and the level of B cell lymphoma-2 (Bcl-2, n = 10) in the uterus mucosa. Scale bar = 50 μm. m Peak plots for flow cytometry analysis in the uterus of hens (n = 10). YU, young uterus; AU, aged uterus; MUC2: Mucin-2; IL-1β: Interleukin-1β; IgG: Immunoglobulin G; IgM: Immunoglobulin M. YE, young liquid whole egg; AE, aged liquid whole egg

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