Fig. 1

Fungi deficiency protects mice against diet-induced obesity. A–E Body weight gain (A), the relative weight of subcutaneous adipose tissue (SAT) and perirenal adipose tissue (PEAT) (%) (B), serum lipids (C), adipocyte size and area of SAT and PEAT (D), and serum glucose (E) in fluconazole (Flu)-treated mice. C57BL/6 mice (6 weeks old, male) were challenged with HFD, or fluconazole lasted for 12 weeks (n = 10–13). SAT and PEAT were staining with HE, and the adipocyte size and area were examined using Case Viewer and ImageJ software (× 400). F and G GTT and ITT and their area under the curve (AUC). C57BL/6 mice were fasted for 10–12 h and subjected to GTT test with intraperitoneal injection of glucose (1 g/kg body weight) and then for ITT test with intraperitoneal injection of insulin (0.75 U/kg body weight) 4 h after restriction. Glucose levels were measured at 15, 30, 60, 90, and 120 min (n = 8). H–J Metabolic parameters (O₂, CO₂, and energy consumption) of HFD- and fluconazole-treated mice were recorded in an International FOXBOX.™ field oxygen analysis system for 48 h, and then the alibrated data were used for calculating the fuel oxidation and respiratory exchange ratio (RER) (n = 8). Differences among the groups were compared using Student’s t-test. *p < 0.05; **p < 0.01; ***p < 0.001; ns p > 0.05