- Anaplasma and Borrelia burgdorferi sensu lato were also tested by PCR in the ticks in contact with the corresponding mice (number of positive ticks in PCR/total tested) and in the mouse skin (+ positive in PCR; - negative in PCR). NA not tested (control mice without ticks); H2O-Ext 10 tubes of molecular grade water extracted, amplified, and sequenced at the same time as the samples; H2O-PCR 12 tubes of molecular grade water amplified and sequenced at the same time as the extracted DNA of the samples