Fig. 3

Impacts on the mouse skin microbiome persist 10 days after the removal of the ticks. A Alpha diversity (cluster levels observed, Shannon, Simpson, and inverse Simpson indexes) of the skin microbiome of control mice without a cup (red dots), control mice with a cup (purple dots), mice bitten 3 days by female ticks (yellow dots), and mice bitten 5 days by nymphs (blue dot). Bitten mice were kept 10 days after the removal of the ticks, before being euthanized. B, C Barplots of relative proportions in the skin biopsies of the phyla (B) and top 20 genera (C). D–G LEfSe (linear discriminant analysis effect size) analysis summarizing the taxa significantly modified (p<0.05 with Mann-Whitney nonparametric test) between skin of control mice without cup and control mice with a cup (D), control mice with a cup and mice bitten by female ticks (E), control mice with a cup and mice bitten by nymphs (F), and mice bitten by female ticks and mice bitten by nymphs (G)