Fig. 1

Shotgun sequencing provides higher accuracy than ITS sequencing in mycobiome profiling at the species level. a Workflow of the mapping depth approach to recover ITS copy numbers. b The distribution of CN-MD (y axis) of the ITS across the strains of the 7 analyzed species (x axis) (n = 260). c The intraspecific CN-MD of S. cerevisiae (x axis) for the 32 strains analyzed (y axis) (n = 32). d In silico comparison at species level between expected abundance (“Expected”) and observed abundance by using the shotgun method (“Shotgun observed”) and the ITS method (“ITS observed”)