Fig. 4

Summary of key metabolic processes in selected bathypelagic prokaryotic communities amended with A thiosulfate and B thiosulfate + DOM. Red font indicates lower transcript abundance in the amended communities as compared to unamended communities. Metabolic pathways drawn in brown belong to the sulfur utilization pathways. Blue plotted pathways are part of the reductive TCA (tricarboxylic acid) cycle. Purple is used to depict biochemical reactions from the Calvin Benson cycle. Green is used to represent reactions involved in the anaplerotic carbon fixation. Gene abbreviations: ACK Acetate kinase, ACL ATP citrate lyase, ActP Acetate permease, Apr Adenylylsulfate reductase, CFA Cyclopropane fatty acid, CL Cardiolipin, CS Citrate synthase, CysK Cysteine synthase, DSR Dissimilatory-type sulfite reductase, FAS Fatty acid biosynthesis, FRD Fumarate reductase, GBE Glycogen branching enzyme, GSS Glutathione synthetase, IM Inner membrane, LamB Maltoporin, OGOR 2-Oxoglutarate:ferredoxin oxidoreductase, OM Outer membrane, OMP Outer membrane protein, oTCA cycle Oxidative tricarboxylic acid cycle, PA Phosphatidic acid, PE Phosphatidylethanolamine, PEPC Phosphoenolpyruvate carboxylase, PG Phosphatidylglycerol, PS Phosphatidylserine, PTA Phosphate acetyltransferase, PTS system, glucose-specific IIBC component, rTCA Reductive tricarboxylic acid cycle, SAT Sulfate adenylyltransferase, Sox Sulfur oxidation protein, SQR Succinate-ubiquinone oxidoreductase, SulP Sulfate transporter