Fig. 3

Effect of Δα-amy cyst infection on gut microbiota assembly. The feces samples (n = 6 per group) for 16S rRNA sequencing analysis were collected on day 10 post-infection in the experiment shown in Fig. 2. A Microbial community abundance (measured by Chao1 index). B Microbial community diversity (measured by Shannon index). C Principal coordinate analysis (PCoA) based on the weighted UniFrac distance matrix. D Relative abundances of fecal bacteria at the genus level. E Heatmap of the top 50 microbiota at the genus level in fecal samples from mice infected with ME49 or Δα-amy cysts. Colour indicates the relative microbiota abundances in the group samples; the corresponding relationship between the colour gradient and the value is shown in the gradient colour block. F Differentially abundant taxa of fecal microbiota between ME49 and Δα-amy cyst-infected mice was analyzed by LEfSe. LDA score ≥ 2