Fig. 4

Virus metagenome sequencing of zebrafish intestinal content. A Alpha diversity (Shannon index) of zebrafish intestinal viruses. B The PCoA plot of zebrafish intestinal viruses. C Relative abundance of viruses at the species level (top 10). D The relative abundance of KT319643 (UNVERIFIED: Listeria phage WIL-3). E The relative abundance of AF191073 (stealth virus 1 clone 3B43). F The relative abundance of KM209255 (Dickeya phage phiDP10.3 clone pD10). G Viruses with significant differences between the 15 + and 15 − group. H Viruses with significant differences between the 150 + and 150 − group. The green arrow represents downregulation, and the red arrow represents upregulation. DESeq2 was used for analysis, and viruses with FDR (false discovery rate) < 0.05 and fold change (FC) ≥ 2 were selected as significant differentially expressed viruses. Data are expressed as means ± SD. *p < 0.05 indicate significant differences between the exposure groups and the control group