Fig. 4

wMel forms a negative interaction hub within the Ae. Aegypti microbiome network. A Microbiome interaction network analysis using Wisconsin normalized read counts resulted in a network of 71 species and 159 edges. Red edges represent mutual exclusion interactions (significant negative Pearson’s correlation coefficient) and green edges represent co-occurrence interactions (significant positive Pearson’s correlation coefficient). Core microbiome species are highlighted in purple. wMel was involved exclusively in negative pairwise interactions with 35 species. B Density plot displaying the strength of interactions edges with wMel (orange) and the remaining edges in the network not involving wMel (grey). C Density plot displaying the mean read count for species included in the network. Species with mutually exclusive interactions with wMel are shown in orange and have a significantly lower average read count (t = 2.43; p-value = 0.02) than all other species in the network (grey)