Fig. 2

Antimicrobial peptide expression is reduced in hyperoxia-exposed intestinal organoids. A Small intestinal spheroid organoids derived from neonatal C57BL/6 J mice were exposed to either hyperoxia or normoxia for 24 h (n = 6 wells/treatment group). B Representative images of organoids before and after exposure, with insets at 40 × magnification. The percentage of organoids with healthy-appearing epithelium declined in hyperoxia-exposed organoids. Data are shown as mean ± SEM, with significance testing by a two-tailed t-test. The scale bar represents 1000 mm. C Representative immunohistochemistry after exposure to normoxia or hyperoxia. Nuclei in blue, actively proliferating cells in green, and lysozyme-positive cells in red. Arrows identify lysozyme-positive Paneth cells. The scale bar represents 25 mm. D Principal components analysis showing differential clustering of normoxia and hyperoxia exposed ileal genes. PC, principal component. E Heatmap showing genes regulated by hyperoxia exposure. F Heatmap of antimicrobial peptide expression is decreased in hyperoxia-exposure organoids. G Ingenuity pathway analysis showing regulated pathways in hyperoxia or normoxia. H Bubble plot showing up and down-regulated pathways from hyperoxia exposure. The schematic in (A) was generated using BioRender